Vertical distribution, environmental occurrence, and risk assessment of organic pollutants in lacustrine sediments in southeast China.

To clarify the impact of human activities on the natural environment, as well as the current ecological risks to the environment surrounding Zhushan Bay in Taihu Lake, the characteristics of deposited organic materials, including elements and 16 polycyclic aromatic hydrocarbons (∑16PAHs), in a sediment core from Taihu Lake were determined. The nitrogen (N), carbon (C), hydrogen (H), and sulfur (S) contents ranged from 0.08 to 0.3%, 0.83 to 3.6%, 0.63 to 1.12%, and 0.02 to 0.24%, respectively. The most abundant element in the core was C followed by H, S, and N, while elemental C and the C/H ratio displayed a decreasing trend with depth. The ∑16PAH concentration was in the range of 1807.48-4674.83 ng g-1, showing a downward trend with depth, with some fluctuations. Three-ring PAHs dominated in surface sediment, while 5-ring PAHs dominated at a depth of 55-93 cm. Six-ring PAHs appeared in the 1830s and gradually increased over time before slowly decreasing from 2005 onward due to the establishment of environmental protection measures. The ratio of PAH monomers indicated that PAHs in samples from a depth of 0 to 55 cm were mainly derived from the combustion of liquid fossil fuels, while the PAHs in the deeper samples mainly originated from petroleum. The results of a principal component analysis (PCA) showed that the PAHs in the sediment core of Taihu Lake were mainly derived from the combustion of fossil fuels, such as diesel, petroleum, gasoline, and coal. The contributions of biomass combustion, liquid fossil fuel combustion, coal combustion, and unknown source were 8.99%, 52.68%, 1.65%, and 36.68%, respectively. The results of a toxicity analysis indicated that most of the PAH monomers had little impact on the ecology, and the annual increase of a small number of monomers might have toxic effects on the biological community, resulting in a serious ecological risks, that requires the imposition of control measures.

Spatial occurrence and sources of PAHs in sediments drive the ecological and health risk of Taihu Lake in China.

To study the spatial occurrence, sources, and ecological risks of 16 PAHs, surface sediments had been collected from seven major areas of Taihu Lake, China in April 2021. Results showed that the concentrations of ∑16PAHs varied between 1381.48 and 4682.16 ng g-1, and the contents of BghiP in each sample were the highest. The PAHs concentrations in the sediments near the lakeshore were much higher than those in the central area of the lake. The sedimentary ∑16PAHs were mainly composed of molecular-weight monomers and 4-ring PAHs showed superiority (35.69-45.02%). According to the ratio of PAH monomer, the sedimentary PAHs in Taihu Lake were dominantly derived from the combustion. Through the biological toxicity assessment and the BaP equivalent (BaPE), great biological risks of PAHs monomers i.e. DahA and IcdP were found. Both concentrations of ∑16PAHs and dominant 4-6-ring monomers accompanied by carcinogenic risks in many areas of Taihu Lake increased. It is necessary to strengthen monitoring and take measures to control the input of organic pollutants.

Seasonal variation of aerosol fungal community structure in reed constructed wetlands.

In recent years, the impact of biological aerosols produced by sewage treatment plants on air quality and human health has become a hot spot of concern. Airborne fungi were characterized via KC-1000 large-flow air sampler and Anderson-type six-stage sampler, at free surface flowing reed constructed wetland located in Qingdao City, Shandong Province. The high-throughput sequencing technology and fungal culture-dependent method were selected to analyze the composition and dynamic changes of the fungal community attached to the atmospheric particulate matter in the free surface flow constructed wetland. The results showed that the aerosol concentration of fungi in the constructed wetlands varied from 587 to approximately 3382 CFU m-3, with a peak at the range of 1.10 to 2.10 µm particle size, and the particles (< 4.70 µm) that easily entered the lungs accounted for 57.03 ~ 96.03%. Significant seasonal differences in fungal richness and community diversity were found. The particle size distribution of fungi in atmospheric particles was not obvious. Fungal genera in the atmospheric particulate matter were mainly driven by humidity. However, other factors, i.e., temperature, NO2, SO2, and PM10 contents, also contributed.

Occurrence characteristics and source appointment of polycyclic aromatic hydrocarbons and n-alkanes over the past 100 years in southwest China.

The extensive anthropogenic activities and their potential impacts during the Anthropocene have led to a research focus on the sedimentary record. In the present study, the occurrence and temporal variations in the fluxes and compositions of n-alkanes and polycyclic aromatic hydrocarbon (PAHs) were investigated in 210Pb-dated sediment cores from a small catchment near the outflow Tanglangchuan in the western Dianchi Lake, China. The continuing organic contamination (i.e. PAHs and n-alkanes) from inputs to outputs has been of concern. To trace the sources and driving forces, multi indicators were applied. Results showed that the total organic carbon (TOC) contents and C/N ratios varied in the range of 4.20-12.30 mg g-1 dw and 8.64-15.65, respectively, indicating algae- and terrestrial plant-derived organic matter (OM). The flux of Σn-alkanes ranged from 0.67 to 38.86 µg cm-2 a-1 with a peak in 2013. The long-chain n-alkanes (Σn-alk26-35) and short-chain n-alkanes (Σn-alk12-20) accounted for 44.02%-49.38% and 35.32%-41.49% of the Σn-alkanes, respectively. A bimodal distribution of n-alkanes was displayed in the sediments implying the sedimentary OM may be derived from a mixed source of endogenous and exogenous origin. The posterior peak (≥n-C26) compounds in the highest abundance were n-C31 or n-C33 with a significant odd-numbered C predominance, representing terrestrial plant-derived OM. Whereas n-C16 was rich in all sediment profiles reflecting crude oil or incompletely combusted fossil fuel-derived source. The indicators analysis showed an increasing trend of the contribution from terrestrial plants and wet to drought climate during 1873-2019. The sedimentary flux of ΣPAHs ranged between 11.71 and 1231.54 ng cm-2 a-1 and the percent of high-ring PAHs rose annually indicating enhanced anthropogenic activities. In the past 147 years, the results of present study highlight the influence of the agricultural and industrial economy on the catchment outlets.

Nitrogen cascade in the agriculture-food-environment system of the Yangtze Delta, 1998-2018.

The nitrogen (N) cascade in rural areas of Changshu County should be measured and evaluated due to the large increase in anthropogenic disturbances in China's Yangtze Delta. Here, we developed a village-scale N flow model using Changshu County and its towns as a case study. The model included four subsystems and was used to describe the driving forces behind the N cascade from agricultural food production and household consumption to the environment (agriculture-food-environment) system. It was found that from 1998 to 2018 the N input increased from 274.63 to 848.65 kg N ha-1. The cropland N use efficiency (NUEc) decreased by 10.35%, whereas the livestock feed N use efficiency (NUEa) increased by 51.84%. A relatively lower NUE, with a higher N input, was found in Shajiabang Town, which was attributed to hairy crab farming. Changes in dietary patterns led to the food N cost (FNC) being in the range of 4.59-7.74 kg kg-1. Over the past two decades, the N losses from the agriculture-food-environment system decreased by 45.40% from 12,436.60 t N yr-1 (1998). The contribution of the croplands, livestock-breeding, and household consumption to the N losses were 32.44%, 37.78%, and 29.78%, respectively. About 62.83% of the total N losses entered the water environment. Nitrogen emissions from the croplands accounted for 63.21% of the N losses into the atmosphere. Nitrogen oxide (NOx) emissions accounted for 38.50% of the gas emissions, followed by NH3 (28.36%) and N2O (2.81%). The total N losses decreased annually but losses to the water environment increased by 5.10% from 60.16% (1998). The contribution of food production to the total N loss displayed a decreasing trend, while that of food consumption exhibited an increasing trend. Population growth and increased volumes of domestic waste in the Changsu area were the main driving forces for the increased contribution of household food consumption. The significant decline in cropland area and increase in built-up and heavily trafficked areas indicated an overall increase in anthropogenic disturbances, stimulating the N cascade in the Yangtze Delta from 1998 to 2018.

Variation of Bacterial and Archaeal Community Structures in a Full-Scale Constructed Wetlands for Wastewater Treatment.

Microorganisms play important roles in the reduction of organic and inorganic pollutants in constructed wetlands used for the treatment of wastewater. However, the diversity and structure of microbial community in constructed wetland system remain poorly known. In this study, the Illumina MiSeq Sequencing of 16S rDNA was used to analyze the bacterial and archaeal microbial community structures of soil and water in a free surface flow constructed wetland, and the differences of bacterial communities and archaeal compositions between soil and water were compared. The results showed that the Proteobacteria were the dominant bacteria, making up 35.38%~48.66% relative abundance. Euryarchaeotic were the absolute dominant archaea in the influent sample with the relative abundance of 93.29%, while Thaumarchaeota showed dominance in the other three samples, making up 50.58%~75.70%. The relative abundances of different species showed great changes in bacteria and archaea, and the number of dominant species in bacteria was much higher than that in archaea. Compared to archaea, the community compositions of bacteria were more abundant and the changes were more significant. Meanwhile, bacteria and archaea had large differences in compositions between water and soil. The microbial richness in water was significantly higher than that in soil. Simultaneously, soil had a significant enrichment effect on some microbial flora.

[Diffusion of Microorganism and Main Pathogenic Bacteria During Municipal Treated Wastewater Discharged into Sea].

Numerous microorganisms, especially pathogens, exist in treated wastewater, which leads to possible risk to population health and marine ecological security when treated wastewater is discharged into the sea. This study selects Maidao municipal sewage treatment plant as the location. The microbial community and the dynamic distribution and seasonal variation of pathogens are analyzed by high-throughput sequencing technology. The results showed that the microbes in the discharge were distributed into 20 to 27 phyla, and the dominant phyla were Proteobacteria, Firmicutes, and Bacteroidetes, which were mainly distributed in 44 to 65 classes. The predominant pathogens are mainly composed of Gammaproteobacteria, Betaproteobacteria, and Epsilonproteobacteria in the Proteobacteria category. The proportion of most dominant phyla and classes in the sample decreased with an increase in diffusion distance. There were mainly 55 species pathogenic bacteria in the water discharging into the sea. The pathogenic genes in the discharge were mainly related to cancer, cardiovascular disease, immune system diseases, infectious diseases, metabolic diseases, and neurodegenerative diseases. When compared with the blank samples, it was found that the biomass of pathogenic bacteria decreased sharply with the diffusion by the seawater, and most of the pathogens were not detected 1000 m away from the seashore. Arcobacter spp. caused human and animal diarrhea and bacteremia, Acinetobacter spp. caused nosocomial infections, and Shewanella hafniensis posed a threat to seafood farming and fishing. The three species of pathogenic bacteria mentioned above occupied a fairly large proportion of the discharge into the sea.

[Influence of Different Patterns of Discharge on Microbial Diffusion in Municipal Treated Wastewater].

Numerous pathogens exist in treated wastewater, leading to possible population health and ecological security risks when treated waste water is reused or discharged. To investigate the influence of different patterns of discharge on microbial diffusion in the municipal treated waste water, high-throughput sequencing technology was used to analyze the bacterial community structure, dominant flora, and typical pathogens. It was found that those bacteria were distributed in 58 classes. There were 32 species with a relative abundance of more than 1%. While there were only 41 classes in the water sample taken as a direct discharge to the sea, and there were 28 species with relative abundances of more than 1%. The water sample collected as a discharge to the sea through a river displayed higher bacterial diversity than the sample collected as a direct discharge to the sea, indicating that the microorganisms in the treated waste water was more likely to diffuse in when directly discharged to the sea. The relative abundance of dominant bacteria decreased with an increase in the diffusion distance, while the relative abundance of Cyanobacteria increased as the diffusion distance increased. The relative abundance in the water sample collected as a discharge to the sea through a river was higher. The dominant bacteria in the two systems are to Proteobacteria, Bacteroidetes, and Firmicutes. However, the dominant bacteria at the class and species levels quite dissimilar in the two systems. The bacterial diversities near the outfall were much higher than that in the seawater (blank sample), and the abundance was higher, which related to sewage treatment. In addition, there was a certain amount of pathogens and potential pathogens, including Pseudoalteromonas haloplanktis and Pseudomonas anguilliseptica, which were highly pathogenic. Their relative abundances were still higher after dilution. Arcobacter spp. was related to human and animal diarrhea and bacterial and other diseases. Therefore, the detection of these types of bacteria when the treated waste water is discharged is very important.