Solvation suppression of ion recombination in gas discharge afterglow.

An effect which suppresses recombination in ion plasmas is considered both theoretically and experimentally. Experimental results are presented for the ion recombination rate in fluorine plasma, which are obtained from data for the gas discharge afterglow. To interpret them, a suppression factor is considered: ion solvation in weakly ionized plasma. It is shown that the recombination process has a two-stage character with the formation of intermediate metastable ion pairs. The pairs consist of negative and positive ion-molecular clusters. A theoretical explanation is given for the slowing down of the ion recombination with the increase of the Coulomb coupling compared to the ion recombination rate calculated in the ideal plasma approximation. The approximate similarity of the recombination rate of the ion temperature and concentration and reasons for the slight deviation from the similarity are elucidated.

Mechanisms of inhibitory effects of polycyclic aromatic hydrocarbons in photosynthetic primary processes in pea leaves and thylakoid preparations.

Inhibitory effects of polycyclic aromatic hydrocarbons (PAHs) on plants were studied in pea leaves in order to elucidate the mechanisms of action of PAHs such as naphthalene (Naph) and phenanthrene (Phen) on activity of photosystem II (PSII). The changes in different Chl fluorescence parameters were calculated on the basis of Chl fluorescence induction curves. H2 O2 content was measured in leaf homogenates with the luminol-dependent chemiluminescence method. We demonstrated that following PAH treatment, total energy dissipation (DI0 /ABS) and amount of QB -non-reducing complexes of PSII significantly increased. Non-photochemical quenching (NPQ) also increased, when weak oxidative stress after PAH application developed. In leaves, a two-step increase in H2 O2 was found with time of incubation in the presence of PAHs, which may be associated with damage to the lipid bilayer of the plasma membrane and then violation of lipid bilayer membranes of cell organelles. A hypothesis for the mode of action of PAHs is provided that involves the role of ROS, membrane permeability and associated functional changes in PSII.

Effect of naphthalene on photosystem 2 photochemical activity of pea plants.

The effect of a typical polyaromatic hydrocarbon, naphthalene (Naph), on photosystem 2 (PS-2) photochemical activity in thylakoid membrane preparations and 20-day-old pea leaves was studied. Samples were incubated in water in the presence of Naph (0.078, 0.21, and 0.78 mM) for 0.5-24 h under white light illumination (15 µmol photons·m(-2)·s(-1)). The PS-2 activity was determined by studying fast and delayed chlorophyll (Chl) a fluorescence. Incubation of samples in water solutions at Naph concentrations of 0.21 and 0.78 mM led to a decrease in the maximum PS-2 quantum efficiency (Fv/Fm), noticeable changes in the polyphasic induction kinetics of fluorescence (OJIP), and a decrease in the amplitudes of the fast and slow components of delayed fluorescence of Chl a. The rate of release of electrolytes from leaves that were preliminarily incubated with Naph (0.21 mM) was also increased. Significant decrease in the fluorescence parameters in thylakoid membrane preparations was observed at Naph concentration of 0.03 mM and 12-min exposure of the samples. Chlorophyll (a and b) and carotenoid content (mg per gram wet mass) was insignificantly changed. The quantum yields of electron transfer from QA to QB (φET2o) and also to the PS-1 acceptors (φRE1o) were reduced. These results are explained by the increase in the number of QB-non-reducing centers of PS-2, which increased with increasing Naph concentration and exposure time of leaves in Naph solution. The suppression of PS-2 activity was partly abolished in the presence of the electron donor sodium ascorbate. Based on these results, it is suggested that Naph distorts cell membrane intactness and acts mainly on the PS-2 acceptor and to a lesser degree on the PS-2 donor side.

Solid-density plasma nanochannel generated by a fast single ion in condensed matter.

A plasma model of relaxation of a medium in heavy-ion tracks in condensed matter is proposed. The model is based on three assumptions: the Maxwell distribution of plasma electrons, localization of plasma inside the track nanochannel, and constant values of the plasma electron density and temperature during the x-ray irradiation. The model of multiple ionization of target atoms by a fast projectile ion is used to determine the initial conditions. An analysis of the results of the calculations performed makes it possible to define when the atomic relaxation model is a very rough approximation and the plasma relaxation model must be used. It is demonstrated that the plasma relaxation model adequately describes the x-ray spectra observed upon interaction of a fast ion with condensed target. The comparison with the experimental data justifies the reliability of the plasma relaxation model. Preassumptions of plasma relaxation model are validated by the molecular-dynamics simulation. An x-ray spectral method based on the plasma relaxation model is proposed for diagnostics of the plasma in fast ion tracks. The results obtained can be useful in examining the initial stage of defect formation in solids under irradiation with single fast heavy ions.