RESUMO
We extend the principles of time-resolved super-resolution source localization from diffractive microscopy to the imaging of objects through scattering media. We show that isolation and localization of the scattering (versus diffractive) point-spread function can be done by individually illuminating or individually darkening image segments. We experimentally demonstrate reconstruction of both bright and dark sources. Further, we show that self-focusing nonlinearity improves the localization accuracy for bright sources.
RESUMO
In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire volumes in the time it takes to perform a single transverse scan (four depths simultaneously at a rate of 30 Hz). In addition, this capability is further exploited to accomplish single-element detection of multiple modalities (two photon excited fluorescence and second harmonic generation) and to perform efficient image deconvolution. Finally, we demonstrate a new system that promises to significantly simplify this promising technology.