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A distraction assembly system (DAS), consisting of a distractor of fully individualized fixation plate unit and factory-engineered distraction unit, was recently developed to enhance the accuracy, stability, and workability of distraction osteogenesis (DO). We here wanted to evaluate the accuracy of DAS for mandibular DO, focusing on the consequences of the distractor design and manufacturing method. The bar-type distractor unit showed smaller discrepancies than did the cylinder-type one, both units showing far fewer discrepancies than those of a conventional distractor. And there were no significant differences in morphological accuracy between the plates produced by milling and 3D printing. These accuracies feature the promising application in clinical setting, and further work is anticipated to refine DAS for successful computer-aided DO.
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OBJECTIVE: The aim of this study was to understand the temporal and spatial distribution of canonical endochondral ossification (CEO) and non-canonical endochondral ossification (NCEO) of the normal growing rat condyle, and to evaluate their histomorphological changes following the simultaneous hypotrophy of the unilateral masticatory closing muscles with botulinum toxin (BTX). DESIGN: 46 rats at postnatal 4 weeks were used for the experiment and euthanized at postnatal 4, 8, and 16 weeks. The right masticatory muscles of rats in experimental group were injected with BTX, the left being injected with saline as a control. The samples were evaluated using 3D morphometric, histological, and immunohistochemical analysis with three-dimensional regional mapping of endochondral ossifications. RESULTS: The results showed that condylar endochondral ossification changed from CEO to NCEO at the main articulating surface during the experimental period and that the BTX-treated condyle presented a retroclined smaller condyle with an anteriorly-shifted narrower articulating surface. This articulating region showed a thinner layer of the endochondral cells, and a compact distribution of flattened cells. These were related to the load concentration, decreased cellular proliferation with thin cellular layers, reduced extracellular matrix, increased cellular differentiation toward the osteoblastic bone formation, and accelerated transition of the ossification types from CEO to NCEO. CONCLUSION: The results suggest that endochondral ossification under loading tended to show more NCEO, and that masticatory muscular hypofunction by BTX had deleterious effects on endochondral bone formation and changed the condylar growth vector, resulting in a retroclined, smaller, asymmetrical, and deformed condyle with thin cartilage.
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Côndilo Mandibular , Músculos da Mastigação , Osteogênese , Animais , Côndilo Mandibular/efeitos dos fármacos , Côndilo Mandibular/crescimento & desenvolvimento , Ratos , Osteogênese/efeitos dos fármacos , Músculos da Mastigação/efeitos dos fármacos , Ratos Wistar , Toxinas Botulínicas/farmacologia , Imuno-Histoquímica , Masculino , Toxinas Botulínicas Tipo A/farmacologiaRESUMO
We present a fully automated method of integrating intraoral scan (IOS) and dental cone-beam computerized tomography (CBCT) images into one image by complementing each image's weaknesses. Dental CBCT alone may not be able to delineate precise details of the tooth surface due to limited image resolution and various CBCT artifacts, including metal-induced artifacts. IOS is very accurate for the scanning of narrow areas, but it produces cumulative stitching errors during full-arch scanning. The proposed method is intended not only to compensate the low-quality of CBCT-derived tooth surfaces with IOS, but also to correct the cumulative stitching errors of IOS across the entire dental arch. Moreover, the integration provides both gingival structure of IOS and tooth roots of CBCT in one image. The proposed fully automated method consists of four parts; (i) individual tooth segmentation and identification module for IOS data (TSIM-IOS); (ii) individual tooth segmentation and identification module for CBCT data (TSIM-CBCT); (iii) global-to-local tooth registration between IOS and CBCT; and (iv) stitching error correction for full-arch IOS. The experimental results show that the proposed method achieved landmark and surface distance errors of 112.4µm and 301.7µm, respectively.
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Tomografia Computadorizada de Feixe Cônico Espiral , Compostos de Trimetilsilil , Humanos , Artefatos , Tomografia Computadorizada de Feixe Cônico , ImidazóisRESUMO
Identification of 3D cephalometric landmarks that serve as proxy to the shape of human skull is the fundamental step in cephalometric analysis. Since manual landmarking from 3D computed tomography (CT) images is a cumbersome task even for the trained experts, automatic 3D landmark detection system is in a great need. Recently, automatic landmarking of 2D cephalograms using deep learning (DL) has achieved great success, but 3D landmarking for more than 80 landmarks has not yet reached a satisfactory level, because of the factors hindering machine learning such as the high dimensionality of the input data and limited amount of training data due to the ethical restrictions on the use of medical data. This paper presents a semi-supervised DL method for 3D landmarking that takes advantage of anonymized landmark dataset with paired CT data being removed. The proposed method first detects a small number of easy-to-find reference landmarks, then uses them to provide a rough estimation of the all landmarks by utilizing the low dimensional representation learned by variational autoencoder (VAE). The anonymized landmark dataset is used for training the VAE. Finally, coarse-to-fine detection is applied to the small bounding box provided by rough estimation, using separate strategies suitable for the mandible and the cranium. For mandibular landmarks, patch-based 3D CNN is applied to the segmented image of the mandible (separated from the maxilla), in order to capture 3D morphological features of mandible associated with the landmarks. We detect 6 landmarks around the condyle all at once rather than one by one, because they are closely related to each other. For cranial landmarks, we again use the VAE-based latent representation for more accurate annotation. In our experiment, the proposed method achieved a mean detection error of 2.88 mm for 90 landmarks using only 15 paired training data.
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Pontos de Referência Anatômicos , Imageamento Tridimensional , Pontos de Referência Anatômicos/anatomia & histologia , Pontos de Referência Anatômicos/diagnóstico por imagem , Cefalometria/métodos , Humanos , Imageamento Tridimensional/métodos , Reprodutibilidade dos Testes , Aprendizado de Máquina Supervisionado , Tomografia Computadorizada por Raios XRESUMO
BACKGROUND: The present study introduces a reduction malarplasty using a three-dimensional (3D)-printed surgical guide and evaluates the guide's technical applicability. METHODS: Twenty malarplasties were performed for 12 subjects with zygomatic asymmetry/prominency using the current method. 3D reconstruction of the craniomaxillofacial region and fine dental occlusion was made with image data from computed tomograpy and dental scanning. A computer-assisted surgical simulation was performed for reduction malarplasty and a surgical guide was designed for later 3D printing. The manufactured surgical guide was introduced to the operation field to guide the surgery; its surgical accuracy was confirmed by comparing five corresponding points from preoperative simulation and postoperative data. RESULTS: We successfully performed the reduction malarplasty with the surgical guide. The accuracy level of surgery fell to 0.93 mm of total median difference for the corresponding zygoma points of preoperative simulations and postoperative zygoma. The anterior and upper points showed less error level (0.59 and 0.73 mm difference, respectively) than did other points. CONCLUSIONS: We developed a computer-assisted surgical technique using a surgical guide for asymmetrical/prominent zygoma which proved to be simple, practical, and accurate; it is expected to help surgeons perform reduction malarplasty with ease and accuracy.
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Procedimentos de Cirurgia Plástica , Zigoma , Humanos , Impressão Tridimensional , Procedimentos de Cirurgia Plástica/métodos , Zigoma/diagnóstico por imagem , Zigoma/cirurgiaRESUMO
INTRODUCTION: Studies on the pharyngeal airway space (PAS) changes using three-dimensional computed tomography (CT) have shed more light on patients with Class III than Class II malocclusion. This paper focuses on analyzing the long-term changes in the PAS and evaluating the postoperative association between these PAS and skeletal changes in patients with skeletal Class II malocclusion who have undergone orthognathic surgery. METHODS: The records of 21 patients with skeletal Class II malocclusion who had undergone orthognathic surgery were included. The anatomical modifications in both jaws, changes in volume, sectional area (SA), minimum sectional area (MSA), and anterior-posterior (AP) and transverse (TV) width in the airway at one month before surgery (T0), and one month (T1) and one year (T2) after surgery were analyzed using CT images. The association between the skeletal and airway changes was evaluated between T0, T1, and T2. RESULTS: After surgery, the ANS, A point, and PNS demonstrated significant posterior and superior movement. The B point and the pogonion exhibited substantial anterior and superior movement. The total and inferior oropharyngeal volumes (vol 3, vol 4) notably increased, while the nasopharyngeal volume (vol 1) decreased. The anterior-posterior movement at the ANS and PNS after surgery was significantly associated with the total volume, vol 2, vol 3, SA 1, MSA, and TV width 1, while substantial association with the total volume was found at the pogonion. CONCLUSION: Thus, an ideal treatment plan can be formulated for patients with skeletal Class II malocclusion by considering the postoperative PAS changes.
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Tomografia Computadorizada de Feixe Cônico , Má Oclusão Classe II de Angle , Procedimentos Cirúrgicos Ortognáticos , Faringe/diagnóstico por imagem , Adulto , Feminino , Humanos , Masculino , Má Oclusão Classe II de Angle/diagnóstico por imagem , Má Oclusão Classe II de Angle/cirurgiaRESUMO
The lengthy time needed for manual landmarking has delayed the widespread adoption of three-dimensional (3D) cephalometry. We here propose an automatic 3D cephalometric annotation system based on multi-stage deep reinforcement learning (DRL) and volume-rendered imaging. This system considers geometrical characteristics of landmarks and simulates the sequential decision process underlying human professional landmarking patterns. It consists mainly of constructing an appropriate two-dimensional cutaway or 3D model view, then implementing single-stage DRL with gradient-based boundary estimation or multi-stage DRL to dictate the 3D coordinates of target landmarks. This system clearly shows sufficient detection accuracy and stability for direct clinical applications, with a low level of detection error and low inter-individual variation (1.96 ± 0.78 mm). Our system, moreover, requires no additional steps of segmentation and 3D mesh-object construction for landmark detection. We believe these system features will enable fast-track cephalometric analysis and planning and expect it to achieve greater accuracy as larger CT datasets become available for training and testing.
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BACKGROUND: A new distraction osteogenesis assembly system comprising a fully customized CAD/CAM-based fixation unit and ready-made distraction unit was developed. The aim of this study was to introduce our new distraction system and to evaluate its accuracy level in a sampled mandibular distraction osteogenesis. METHODS: Our system consists of a fully customized CAD/CAM-based fixation plate unit with two plates for each moving and anchoring part, and a ready-made distraction unit with attachment slots for fixation plates. The experimental distractions were performed on 3D-printed mandibles for one control and two experimental groups (N = 10 for each group). All groups had reference bars on the chin region and teeth to measure distraction accuracy. The control group had the classical ready-made distraction system, and experimental groups 1 and 2 were fitted with our new distraction assembly using a different distractor-positioning guide design. All distracted experimental mandibles were scanned by CT imaging, then superimposed on a 3D simulation to get their discrepancy levels. RESULTS: The measured 3D distances between the reference landmarks of the surgical simulations and the experimental surgeries for the three groups were significantly different (p < 0.0001) by statistical analysis. The errors were greater in the control group (with a total average of 19.18 ± 3.73 mm in 3D distance between the simulated and actual reference points) than those in the two experimental groups (with an average of 3.68 ± 1.41 mm for group 1 and 3.07 ± 1.39 mm for group 2). The customized distraction assembly with 3D-printed bone plate units in group 1 and 2, however, did not show any significant differences between simulated and actual distances (p > 0.999). CONCLUSION: Our newly-developed distraction assembly system with CAD/CAM plate for the distraction osteogenesis of the mandible produced a greater level of accuracy than that of a conventional distraction device. The system appears to address existing shortcomings of conventional distraction devices, including inaccuracy in vector-controlled movement of the system. However, it also needs to be further developed to address the requirements and anatomical characteristics of specific regions.
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Osteogênese por Distração , Placas Ósseas , Desenho Assistido por Computador , Mandíbula/diagnóstico por imagem , Mandíbula/cirurgia , OsteogêneseRESUMO
The annotation of three-dimensional (3D) cephalometric landmarks in 3D computerized tomography (CT) has become an essential part of cephalometric analysis, which is used for diagnosis, surgical planning, and treatment evaluation. The automation of 3D landmarking with high-precision remains challenging due to the limited availability of training data and the high computational burden. This paper addresses these challenges by proposing a hierarchical deep-learning method consisting of four stages: 1) a basic landmark annotator for 3D skull pose normalization, 2) a deep-learning-based coarse-to-fine landmark annotator on the midsagittal plane, 3) a low-dimensional representation of the total number of landmarks using variational autoencoder (VAE), and 4) a local-to-global landmark annotator. The implementation of the VAE allows two-dimensional-image-based 3D morphological feature learning and similarity/dissimilarity representation learning of the concatenated vectors of cephalometric landmarks. The proposed method achieves an average 3D point-to-point error of 3.63 mm for 93 cephalometric landmarks using a small number of training CT datasets. Notably, the VAE captures variations of craniofacial structural characteristics.
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Pontos de Referência Anatômicos , Cefalometria , Imageamento Tridimensional/normas , Aprendizado de Máquina , Automação , Humanos , Reprodutibilidade dos Testes , Crânio/anatomia & histologia , Crânio/diagnóstico por imagem , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVE: The purpose of this study was to evaluate the accuracy of an optical tracking system during reference point localization, measurement, and registration of skull models for navigational maxillary orthognathic surgery. STUDY DESIGN: Accuracy was first evaluated on the basis of the position recording discrepancy at a static point and at 2 points of fixed lengths. Ten reference points were measured on a skull model at 7 different locations, and their measurements were compared with predicted positions by using 4 registration methods. Finally, positional tracking of reference points for simulated maxillary surgery was performed and compared with laser scan data. RESULTS: The average linear measurement discrepancy was 0.28 mm, and the mean measurement discrepancy with the 5 registered cranial points was 1.53 mm. The average measurement discrepancy after maxillary surgery was 1.91 mm (for impaction) and 1.56 mm (for advancement). The registration discrepancy in jitter and point registration on the y-axis was significantly greater than on the other axes. CONCLUSIONS: The optical tracking system seems clinically acceptable for precise tracking of the maxillary position during navigational orthognathic surgery, notwithstanding the chance of greater measurement error on the y-axis.
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Procedimentos Cirúrgicos Ortognáticos , Cirurgia Assistida por Computador , Imageamento Tridimensional , Maxila , Cirurgia OrtognáticaRESUMO
The purpose of this study was to investigate the relationship between masticatory muscular hypotrophy and mandibular growth in juvenile nonhuman primates (cynolmolgus monkeys, Macaca fasicularis). We hypothesized that botulinum toxin (BTX)-induced neuro-muscular junctional block and its resultant hypotrophy of masticatory muscles would produce mandibular growth disturbances in size and shape. Ten male cynomolgus monkeys were divided into three groups: group I (control; n = 3), group II (unilateral BTX; n = 4), and group III (bilateral BTX; n = 3). The unilateral or bilateral muscular hypotrophy of major masticatory closing muscles was induced by synchronous BTX application to masseter, medial pterygoid, and temporal muscle. Mandibular growth was tracked by linear, angular, area and volume measurements using three-dimensional (3D) computed tomography imaging before BTX treatment and after 3 and 6 months. After unilateral hypotrophy of masticatory muscles in group II, vertical growth deficiency was prominent on the BTX side, with compensatory overgrowth on the control side. The bilateral muscular hypotrophy in group III also showed smaller ramal height and width than that of control (group I) and control side (group II). Moreover, ramal sagittal angles (posterior tilt) increased on the BTX side of both groups II and III, but coronal angles (lateral tilt) did so on the BTX side of group II, resulting in asymmetry. The results confirmed our hypothesis that functional activity of masticatory closing muscles is closely related to mandibular growth in size and shape of juvenile nonhuman primates. In addition, the focused growth disturbances on the ramal height and posterior-lateral tilt suggested the possible role of masticatory closing muscles for ramal vertical and angular growth vector of the mandible.
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INTRODUCTION: Intraoperative mobile Cone-Beam Computed Tomography (CBCT) trends to develop for the management of complex facial fractures. It allows a real-time imaging and surgical navigation. AIM: Through the presentation of two clinical cases, we aimed at presenting the procedure of intra-operative CBCT and new applications in maxillofacial surgery. RESULTS: A young patient with extended orbito-frontal fibrous dysplasia, and a child with the recurrence of a temporomandibular joint ankylosis secondary to mastoïditis, were operated using a intra-operative imaging control. In both cases, the intraoperative CBCT increased precision and safety of the bone resection. No surgical complication was noted and a good healing was obtained. CONCLUSION: Intraoperative CBCT raises the problems of radiation exposure and increased operating time. However, it represents a useful imaging tool and a navigation system in complex situations as osseous dysplasia and surgery of the temporo-mandibular joint.
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Anquilose , Fraturas Cranianas , Cirurgia Bucal , Transtornos da Articulação Temporomandibular , Criança , Tomografia Computadorizada de Feixe Cônico , Humanos , Imageamento TridimensionalRESUMO
OBJECTIVE: The purpose of this study was to investigate changes in the dentoalveolus and occlusal plane associated with the hypotrophy of unilateral masticatory muscles following botulinum toxin (BTX) treatment in the juvenile period of rats. DESIGN: We hypothesized that the loss of functional loading of masticatory muscles and occlusal force invites compensatory dentoalveolar supraeruption, accelerating occlusal cant and skeletal asymmetry. In order to confirm this hypothesis, six-week-old male rats (N = 5) were treated with BTX simultaneously at the unilateral masseter, temporalis, and medial pterygoid muscles, with a booster injection after six weeks for the experimental group. The control group (N = 6) had saline injections on both sides at the same sites and on the same schedule. RESULTS: After 12 weeks, masseter and medial pterygoid muscles on the BTX side showed hypotrophic change. The mandibular structure was asymmetrical, with decreased size and lateral tilting. The maxillary and mandibular molars were supraerupted from the Frankfort plane or mandibular inferior border with lateral tilt. They accompanied downward occlusal plane cant resulting from the supraerupted maxillary and mandibular molars on the BTX side. The dentoalveolar structural changes included diminished alveolar bone density, narrow periodontal ligament space, and disorganized distribution of periodontal collagen fiber. CONCLUSIONS: Unilateral hypotrophy of masticatory muscles affected the growth, symmetry, and structure of the skeletal jaws and dentoalveolus. Our hypothesis about the dentoalveolar compensation, that muscular hypotrophy was closely integrated with dentoalveolar supraeruption and an inclined occlusal plane, was confirmed.
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Toxinas Botulínicas/administração & dosagem , Músculo Masseter/patologia , Músculos da Mastigação/patologia , Erupção Dentária , Animais , Força de Mordida , Clostridium botulinum , Oclusão Dentária , Masculino , Músculo Masseter/microbiologia , Músculos da Mastigação/microbiologia , RatosRESUMO
This paper presents a new approach to automatic three-dimensional (3D) cephalometric annotation for diagnosis, surgical planning, and treatment evaluation. There has long been considerable demand for automated cephalometric landmarking, since manual landmarking requires considerable time and experience as well as objectivity and scrupulous error avoidance. Due to the inherent limitation of two-dimensional (2D) cephalometry and the 3D nature of surgical simulation, there is a trend away from current 2D to 3D cephalometry. Deep learning approaches to cephalometric landmarking seem highly promising, but there exist serious difficulties in handling high dimensional 3D CT data, dimension referring to the number of voxels. To address this issue of dimensionality, this paper proposes a shadowed 2D image-based machine learning method which uses multiple shadowed 2D images with various lighting and view directions to capture 3D geometric cues. The proposed method using VGG-net was trained and tested using 2700 shadowed 2D images and corresponding manual landmarkings. Test data evaluation shows that our method achieved an average point-to-point error of 1.5 mm for the seven major landmarks.
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Pontos de Referência Anatômicos , Cefalometria/métodos , Imageamento Tridimensional/normas , Aprendizado de Máquina , Automação , Humanos , Reprodutibilidade dos TestesRESUMO
PURPOSE: The goal of this study was to understand the three-dimensional (3D) structural characteristics of hemifacial microsomia (HFM) mandible in terms of skeletal units, especially to locate the underdeveloped skeletal regions for treatment. Another goal was to compare the HFM structure of different age groups to understand growth potential relevant to treatment scheduling. MATERIALS AND METHODS: We reconstructed 3D mandibles from computed tomographic images of French and Korean patients with HFM (N = 28; group II) and normal subjects (N = 27; group I). Each mandible was classified by Pruzansky's HFM types I, II and III, and by age group (child, adolescent, and adult). The mandible was divided into skeletal units, and geometrical representation by skeletal unit line was performed, including the condylar, body, coronoid, and angular units. Their length and angulations were measured and statistically analyzed. RESULTS: The results showed that the affected condylar unit in type II HFM and the condylar/coronoid unit in type III were smaller in young age groups than were other units. The angulation between the skeletal units in type II, though not type III, tended toward normalcy with age, but not to the normal degree of angulations in group I. CONCLUSION: Our study shows the major involvement of condylar unit and minor involvement of body unit for HFM, improving with age in type II. The mandibular skeletal unit analysis seems to be a useful tool for individualized diagnosis, allowing identification of the major etiopathogenic area and treatment planning, including a simulation to set up a regimen for successful reconstruction of HFM.
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Síndrome de Goldenhar/diagnóstico por imagem , Mandíbula/diagnóstico por imagem , Adolescente , Adulto , Fatores Etários , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Imageamento Tridimensional , Masculino , Côndilo Mandibular/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adulto JovemRESUMO
The epithelial disintegration and the mesenchymal bridging are critical steps in the fusion of facial prominences during the upper lip development. These processes of epithelial-mesenchymal transition and programmed cell death are mainly influenced by Wnt signals. Axis inhibition protein2 (Axin2), a major component of the Wnt pathway, has been reported to be involved in lip development and cleft pathogenesis. We wanted to study the involvement of Axin2 in the lip development, especially during the epithelial disintegration of facial prominences. Our results show that Axin2 was expressed mainly in the epithelium of facial prominences and decreased when the prominences were about to contact each other between Hamburger-Hamilton stages 27 and 28 of chicken embryos. The epithelial integrity was destructed or kept intact by the local gain or loss of Axin2 expression, resulting in morphological changes in the facial processes and their skeletal derivatives including the maxilla, nasal, premaxilla bone, and their junctions without cleft formation. These changes were related to expression changes in nuclear ß-catenin, pGSK3ß, Slug, Smad3, E-cadherin, and p63. All these data indicate that Axin2 participates in the regulation of epithelial integrity and fusion by promoting epithelial disassociation, basement membrane breakdown, and seam loss during the fusion of facial prominences in lip development.
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Proteínas Aviárias/genética , Proteína Axina/genética , Bico/embriologia , Galinhas/crescimento & desenvolvimento , Galinhas/genética , Regulação da Expressão Gênica no Desenvolvimento , Morfogênese , Animais , Bico/metabolismo , Morte Celular , Embrião de Galinha/citologia , Embrião de Galinha/metabolismo , Galinhas/metabolismoRESUMO
This study aimed to divide the mandible into skeletal units based on three-dimensional (3D) muscular anatomy with microcomputed tomography (micro-CT) of Sprague-Dawley rat. Five normal rats were micro-CT scanned at 12 weeks of age before and after contrast enhancements for the masticatory muscles. Three-dimensional reconstruction of the mandible was performed from the initial micro-CT images, followed by segmentation of the masticatory muscles using the second enhanced micro-CT data. Bone and muscle models were superimposed based on the teeth and bony structures to evaluate muscular orientation and attachment. The mandible was divided into skeletal units using the bony structures and muscle attachments. The mandibular foramen and mental foramen were adopted as the reference points based on their anatomical and developmental significance. The skeletal units consisted of the condylar, coronoid, angular, body and symphyseal units. Further evaluation of these units in relation to development, growth, and other biology and medicine will be helpful in elucidating their biological identities.
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Cefalometria/métodos , Mandíbula/anatomia & histologia , Músculos da Mastigação/anatomia & histologia , Animais , Imageamento Tridimensional/métodos , Masculino , Modelos Anatômicos , Ratos , Ratos Sprague-Dawley , Microtomografia por Raio-XRESUMO
PURPOSE: The aim of the present study was to compare the long-term stability of bimaxillary surgery using an intraoral vertical ramus osteotomy (IVRO) with and without presurgical orthodontic treatment. MATERIALS AND METHODS: The present retrospective study included 31 consecutive patients with skeletal Class III malocclusions who had undergone bimaxillary surgery (Le Fort I osteotomy and bilateral IVRO). Patients were divided into 2 groups based on treatment type: pre-orthodontic orthognathic surgery (POGS; n = 17) and conventional surgery with presurgical orthodontic treatment (CS; n = 14). Lateral cephalograms were obtained before surgery, 1 day after surgery, 1 month after surgery, 1 year after surgery, and 2 years after surgery to evaluate skeletal and soft tissue changes between the 2 groups. Data were analyzed using χ2 tests, Mann-Whitney U tests, repeated-measures analyses of variance, and independent t tests. RESULTS: There was no significant difference in skeletal or soft tissue measurements-with the exception of the angle between the sella-and-nasion plane and the occlusal plane (SN-OP; P < .001)-between the CS and POGS groups at 2 years after IVRO. The SN-OP had increased in the CS group but decreased in the POGS group at 2 years after surgery. CONCLUSIONS: These findings suggest that POGS and CS have similar long-term stability in patients with skeletal Class III malocclusion.
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Má Oclusão Classe III de Angle/cirurgia , Osteotomia Maxilar/métodos , Procedimentos Cirúrgicos Ortognáticos/métodos , Adolescente , Cefalometria , Feminino , Humanos , Masculino , Ortodontia Corretiva , Osteotomia de Le Fort , Osteotomia Sagital do Ramo Mandibular/métodos , Estudos Retrospectivos , Resultado do Tratamento , Adulto JovemRESUMO
OBJECTIVE: We wanted to evaluate the three-dimensional (3D) mandibular growth of Sprague-Dawley rats from 4th to 16th postnatal weeks with periodic and live micro-computed tomographic scanning. DESIGN: Twenty Sprague-Dawley rats were used for micro-CT scanning from 4th to 16th postnatal weeks. After 3D reconstruction of rat mandible, we performed the linear and angular measurements and the superimposition of the 3D models to evaluate the mandibular growth of rat. RESULTS: The results showed that the growth direction of the condylar and coronoid regions was superior primarily and posterior secondarily, while the condyle had minimal lateral growth. Moreover, the angular region was growing mainly toward the posterior and lateral direction, while the body and symphysis maintained small, incremental anterior-posterior growth. CONCLUSIONS: We could evaluate the amount, rate, and direction of growth using the mandibular skeletal unit. Some reference points and measurements were more relevant in properly characterizing 3D growth of the mandible. Their growth rates were the greatest between 4th and 8th postnatal weeks, a period which seems most appropriate for studies of rat mandible growth.
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Imageamento Tridimensional , Mandíbula/crescimento & desenvolvimento , Microtomografia por Raio-X , Animais , Masculino , Mandíbula/diagnóstico por imagem , Ratos , Ratos Sprague-DawleyRESUMO
BACKGROUND: Syngnathia is a congenital craniofacial disorder characterized by bony or soft tissue fusion of upper and lower jaws. Previous studies suggested some causative signals, such as Foxc1 or Bmp4, cause the disruption of maxillomandibular identity, but their location and the interactive signals involved remain unexplored. We wanted to examine the embryonic origin of syngnathia based on the assumption that it may be located at the separation between the maxillary and mandibular processes. This region, known as the maxillomandibular junction (MMJ), is involved in segregation of cranial neural crest-derived mesenchyme into the presumptive upper and lower jaws. RESULTS: Here we investigated the role of Fgf, Bmp, and retinoid signaling during development of MMJ in chicken embryos. By changing the levels of these signals with bead implants, we induced syngnathia with microstomia on the treated side, which showed increased Barx1 and neural cell adhesion molecule (NCAM) expression. Redistribution of proliferating cells was also observed at the proximal region to maxillary and mandibular arch around MMJ. CONCLUSIONS: We propose that interactive molecular signaling by Fgfs, Bmps, and retinoids around MMJ is required for normal separation of the maxilla and mandible, as well as the proper positioning of beak commissure during early facial morphogenesis. Developmental Dynamics 246:28-40, 2017. © 2016 Wiley Periodicals, Inc.