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1.
Environ Sci Ecotechnol ; 23: 100492, 2025 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-39398413

RESUMO

Electrochemical nitrate reduction to ammonia (ENRA) is gaining attention for its potential in water remediation and sustainable ammonia production, offering a greener alternative to the energy-intensive Haber-Bosch process. Current research on ENRA is dedicated to enhancing ammonia selectively and productivity with sophisticated catalysts. However, the performance of ENRA and the change of catalytic activity in more complicated solutions (i.e., nitrate-polluted groundwater) are poorly understood. Here we first explored the influence of Ca2+ and bicarbonate on ENRA using commercial cathodes. We found that the catalytic activity of used Ni or Cu foam cathodes significantly outperforms their pristine ones due to the in situ evolution of new catalytic species on used cathodes during ENRA. In contrast, the nitrate conversion performance with nonactive Ti or Sn cathode is less affected by Ca2+ or bicarbonate because of their original poor activity. In addition, the coexistence of Ca2+ and bicarbonate inhibits nitrate conversion by forming scales (CaCO3) on the in situ-formed active sites. Likewise, ENRA is prone to fast performance deterioration in treating actual groundwater over continuous flow operation due to the presence of hardness ions and possible organic substances that quickly block the active sites toward nitrate reduction. Our work suggests that more work is required to ensure the long-term stability of ENRA in treating natural nitrate-polluted water bodies and to leverage the environmental relevance of ENRA in more realistic conditions.

2.
Arch Osteoporos ; 19(1): 95, 2024 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-39377968

RESUMO

A retrospective comparative study revealed that percutaneous kyphoplasty combined with pediculoplasty (PKCPP) offers more benefits in terms of pain relief, spinal stability, and complications compared to simple percutaneous kyphoplasty. Moreover, PKCPP can augment and internally fixate the severe osteoporotic vertebral fractures. PURPOSE: Vertebral augmentation (VA) has emerged as a satisfactory and minimally invasive surgical approach for severe osteoporotic vertebral fractures (OVFs). However, treating severe OVFs with advanced collapse, burst morphology with MC injury, posterior wall retropulsion, high degree of osseous fragmentation, pediculo-somatic junction fracture, and large vacuum cleft presents significant challenges. This study aimed to evaluate the effectiveness of percutaneous kyphoplasty combined with pediculoplasty (PKCPP) in reducing refracture, preventing further collapse and bone cement displacement, reconstructing vertebral body (VB) stability, and providing internal fixation of the anterior column (AC), middle column (MC), and the bilateral pedicles. METHODS: The current study was designed as a retrospective review of clinical and radiologic parameters. From July 2018 to September 2021, ninety-six patients with severe OVFs and without neurological deficit were treated either with simple percutaneous kyphoplasty (simple PKP group, n = 54) or with percutaneous kyphoplasty combined with pediculoplasty (PKCPP group, n = 42). All patients were followed up for at least 1 year, and clinical and radiological outcomes were assessed. Surgery duration and bone cement volume were compared between the two groups, as well as analgesic dosage and hospital stay. Anterior wall height (AWH), posterior wall height (PWH), and Cobb angle (CA) were measured and analyzed before and after surgery. RESULTS: The simple PKP group had significantly shorter surgery duration and lower bone cement volume compared to the PKCPP group (P < 0.05). Conversely, the simple PKP group had significantly higher analgesic dosage and longer hospital stay than the PKCPP group (P < 0.05). Both groups showed significant improvements in AWH, PWH, and CA after surgery (P < 0.05). At the final follow-up, the PWH in the simple PKP group was significantly lower than the preoperative measurement (P < 0.05), and the difference in PWH between the two groups was statistically significant (P > 0.05). Moreover, both groups demonstrated a significant reduction in CA after surgery, with the PKCPP group showing a greater reduction compared to the simple PKP group throughout the postoperative period to the final follow-up (P < 0.05). VAS and ODI scores significantly decreased in both groups after surgery (P < 0.05), with no significant difference between the groups at the final follow-up (P > 0.05). However, the PKCPP group achieved better VAS scores than the simple PKP group at postoperative 1 day, 1 month, and 3 months (P < 0.05), and the ODI in the PKCPP group was lower than the simple PKP group at 1 month after surgery (P < 0.05). Furthermore, the overall complication rate in the PKCPP group was significantly lower than that in the simple PKP group (P < 0.05). CONCLUSION: If performed by appropriately trained surgeons, both PKP and PKCPP are safe and effective treatments for patients with severe OVFs. However, PKCPP offers additional benefits in the setting of bothersome fractures, including rapid pain relief, improved spinal stability, satisfactory restoration of vertebral body height, and better correction of kyphotic deformity. These promising results have been tested in a single center but require further confirmation in multiple centers.


Assuntos
Fixação Interna de Fraturas , Cifoplastia , Fraturas por Osteoporose , Fraturas da Coluna Vertebral , Humanos , Estudos Retrospectivos , Fraturas por Osteoporose/cirurgia , Feminino , Masculino , Fraturas da Coluna Vertebral/cirurgia , Idoso , Cifoplastia/métodos , Fixação Interna de Fraturas/métodos , Pessoa de Meia-Idade , Resultado do Tratamento , Cimentos Ósseos/uso terapêutico , Idoso de 80 Anos ou mais , Vértebras Lombares/cirurgia
3.
Molecules ; 29(19)2024 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-39407561

RESUMO

Cinnamides are common core structures that exist in a great number of pharmaceuticals and natural products. The development of efficient methods for preparing cinnamides is in great need. We report herein an efficient polyphosphoric acid (PPA)-promoted direct aldol condensation of an amide for the convenient and straightforward preparation of cinnamides. A variety of cinnamides were obtained in moderate-to-excellent yields (65-89%). This strategy features the use of equivalent amides and a short reaction time.

4.
Sci China Life Sci ; 2024 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-39231901

RESUMO

The innovation of CRISPR/Cas gene editing technology has developed rapidly in recent years. It is widely used in the fields of disease animal model construction, biological breeding, disease diagnosis and screening, gene therapy, cell localization, cell lineage tracking, synthetic biology, information storage, etc. However, developing idealized editors in various fields is still a goal for future development. This article focuses on the development and innovation of non-DSB editors BE and PE in the platform-based CRISPR system. It first explains the application of ideas for improvement such as "substitution", "combination", "adaptation", and "adjustment" in BE and PE development and then catalogues the ingenious inversions and leaps of thought reflected in the innovations made to CRISPR technology. It will then elaborate on the efforts currently being made to develop small editors to solve the problem of AAV overload and summarize the current application status of editors for in vivo gene modification using AAV as a delivery system. Finally, it summarizes the inspiration brought by CRISPR/Cas innovation and assesses future prospects for development of an idealized editor.

5.
Fish Shellfish Immunol ; 154: 109904, 2024 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-39276813

RESUMO

Golden pompano (Trachinotus ovatus), a marine farmed fish, is economically valuable in China. Lysophosphatidic acid phosphatase type 6 (ACP6) is a type of histidine acid phosphatase and plays an important role in regulating host inflammatory responses and anti-cancer effects in mammals. However, its function in teleost remains unknown. The present study aimed to investigate ACP6 function in golden pompano. ACP6 from golden pompano was identified, cloned, and named TroACP6. The open reading frame of TroACP6 was 1275 bp in length, encoding 424 amino acids. The TroACP6 protein shared high sequence identity (43.32%-90.57 %) with the ACP6 of other species. It contained a histidine phosphatase domain with the active site motif "RHGART" and the catalytic dipeptide HD (histidine and aspartate). Meanwhile, TroACP6 mRNA was widely distributed in the various tissues of healthy golden pompano, with the maximum expression in the head kidney. The function of TroACP6 was analyzed both in vitro and in vivo, and the results revealed that the purified recombinant TroACP6 protein exhibited optimum phosphatase activity at pH 6.0 and 50 °C in vitro. Meanwhile, upon Edwardsiella tarda challenge, TroACP6 expression in tissues increased significantly in vivo. In addition, TroACP6 overexpression enhanced the respiratory burst activity and superoxide dismutase activity of head kidney macrophages in vivo. Furthermore, the overexpression and knockdown of TroACP6 in vivo had a significant effect on bacterial infection. In summary, the study findings indicate that TroACP6 in golden pompano is involved in host defense against bacterial infection.

6.
Nanomedicine (Lond) ; 19(24): 1977-1993, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39225145

RESUMO

Aim: To evaluate the anti-pancreatic cancer effect of novel Tubeimoside I multifunctional liposomes combined with gemcitabine.Methods: Liposomes were prepared through the thin film hydration method, with evaluations conducted on parameters including encapsulation efficiency (EE%), particle size, polydispersity index (PDI), zeta potential (ZP), storage stability, and release over a 7-day period. The cellular uptake rate, therapeutic efficacy in vitro and in vivo and the role of immune microenvironment modulation were evaluated.Results: The novel Tubeimoside I multifunctional liposomal exhibited good stability, significant anti-cancer activity, and immune microenvironment remodeling effects. Furthermore, it showed a safety profile.Conclusion: This study underscores the potential of Novel Tubeimoside I multifunctional liposomal as a promising treatment option for pancreatic cancer.


[Box: see text].


Assuntos
Desoxicitidina , Sistemas de Liberação de Medicamentos , Gencitabina , Lipossomos , Neoplasias Pancreáticas , Desoxicitidina/análogos & derivados , Desoxicitidina/farmacologia , Desoxicitidina/administração & dosagem , Desoxicitidina/química , Desoxicitidina/uso terapêutico , Neoplasias Pancreáticas/tratamento farmacológico , Lipossomos/química , Humanos , Animais , Linhagem Celular Tumoral , Sistemas de Liberação de Medicamentos/métodos , Triterpenos/química , Triterpenos/farmacologia , Triterpenos/administração & dosagem , Saponinas/química , Saponinas/farmacologia , Saponinas/administração & dosagem , Tamanho da Partícula , Camundongos , Liberação Controlada de Fármacos , Antineoplásicos/farmacologia , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Antineoplásicos/uso terapêutico
7.
J Hazard Mater ; 478: 135505, 2024 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-39146587

RESUMO

Struvite recovery shows significant potential for simultaneously recovering nitrogen (N) and phosphorus (P) from swine wastewater but is challenged by the occurrence and transformation of antibiotic residuals. Electrochemically mediated struvite precipitation with sacrificial magnesium anode (EMSP-Mg) is promising due to its automation and chemical-free merits. However, the fate of antibiotics remains underexplored. We investigated the behavior of sulfadiazine (SD), an antibiotic frequently detected but less studied than others within the EMSP-Mg system. Significantly less SD (≤ 5%) was co-precipitated with recovered struvite in EMSP-Mg than conventional chemical struvite precipitation (CSP) processes (15.0 to 50.0%). The reduced SD accumulation in struvite recovered via EMSP was associated with increased pH and electric potential differences, which likely enhanced the electrostatic repulsion between SD and struvite. In contrast, the typical strategies used in enhancing P removal in the EMSP-Mg system, including increasing the Mg/P ratio or the Mg-release rates, have shown negligible effects on SD adsorption. Furthermore, typical coexisting ions (Ca2+, Cl-, and HCO3-) inhibited SD adsorption onto recovered products. These results provide new insights into the interactions between antibiotics and struvite within the EMSP-Mg system, enhancing our understanding of antibiotic migration pathways and aiding the development of novel EMSP processes for cleaner struvite recovery.


Assuntos
Eletrodos , Magnésio , Estruvita , Águas Residuárias , Poluentes Químicos da Água , Estruvita/química , Animais , Águas Residuárias/química , Magnésio/química , Suínos , Poluentes Químicos da Água/química , Fósforo/química , Sulfadiazina/química , Antibacterianos/química , Compostos de Magnésio/química , Precipitação Química , Eliminação de Resíduos Líquidos/métodos
8.
Molecules ; 29(16)2024 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-39202795

RESUMO

Significant interest has emerged for the application of Pd-In2O3 catalysts as high-performance catalysts for CO2 hydrogenation to CH3OH. However, precise active site control in these catalysts and understanding their reaction mechanisms remain major challenges. In this investigation, a series of Pd-InOx catalysts were synthesized, revealing three distinct types of active sites: In-O, Pd-O(H)-In, and Pd2In3. Lower Pd loadings exhibited Pd-O(H)-In sites, while higher loadings resulted in Pd2In3 intermetallic compounds. These variations impacted catalytic performance, with Pd-O(H)-In catalysts showing heightened activity at lower temperatures due to the enhanced CO2 adsorption and H2 activation, and Pd2In3 catalysts performing better at elevated temperatures due to the further enhanced H2 activation. In situ DRIFTS studies revealed an alteration in key intermediates from *HCOO over In-O bonds to *COOH over Pd-O(H)-In and Pd2In3 sites, leading to a shift in the main reaction pathway transition and product distribution. Our findings underscore the importance of active site engineering for optimizing catalytic performance and offer valuable insights for the rational design of efficient CO2 conversion catalysts.

9.
Molecules ; 29(16)2024 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-39202989

RESUMO

Phosphogypsum is a solid waste with great environmental stockpile pressure produced by the wet production of phosphoric acid. Although there are various methods to purify and utilize phosphogypsum, the means for environmentally friendly, low energy consumption, and high value-added utilization still need to be further explored. Here, CaSO4·2H2O crystal was directly purified and regulated from phosphogypsum by using the anti-solvent method. The antisolvent can be adsorbed in the c-axis direction of the crystal and further inhibit the growth rate in this direction, resulting in a change in the morphology of the crystal. By adjusting the polarity and chain length of the anti-solvent, the morphology of CaSO4·2H2O crystal can change from butterfly-like flake crystals to hexagonal prism-like crystals. When n-propanol with long chain was used as the anti-solvent, the morphology of the CaSO4·2H2O crystal showed a hexagonal prism with a specific surface area of 19.98 m2/g and a Cu2+ loading efficiency of 52.67%. The encouraging results open up new possibilities for the application of phosphogypsum.

10.
iScience ; 27(8): 110458, 2024 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-39108717

RESUMO

Acute myeloid leukemia (AML) is highly heterogeneous, necessitating personalized prognosis prediction and treatment strategies. Many of the current patient classifications are based on molecular features. Here, we classified the primary AML patients by predicted death risk curves and investigated the survival-directly-related molecular features. We developed a deep learning model to predict 5-year continuous-time survival probabilities for each patient and converted them to death risk curves. This method captured disease progression dynamics with high temporal resolution and identified seven patient groups with distinct risk peak timing. Based on clusters of death risk curves, we identified two robust AML prognostic biomarkers and discovered a subgroup within the European LeukemiaNet (ELN) 2017 Favorable category with an extremely poor prognosis. Additionally, we developed a web tool, De novo AML Prognostic Prediction (DAPP), for individualized prognosis prediction and expression perturbation simulation. This study utilized deep learning-based continuous-time risk modeling coupled with clustering-predicted risk distributions, facilitating dissecting time-specific molecular features of disease progression.

11.
Sci Adv ; 10(32): eado1739, 2024 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-39121223

RESUMO

During lagging strand chromatin replication, multiple Okazaki fragments (OFs) require processing and nucleosome assembly, but the mechanisms linking these processes remain unclear. Here, using transmission electron microscopy and rapid degradation of DNA ligase Cdc9, we observed flap structures accumulated on lagging strands, controlled by both Pol δ's strand displacement activity and Fen1's nuclease digestion. The distance between neighboring flap structures exhibits a regular pattern, indicative of matured OF length. While fen1Δ or enhanced strand displacement activities by polymerase δ (Pol δ; pol3exo-) minimally affect inter-flap distance, mutants affecting replication-coupled nucleosome assembly, such as cac1Δ and mcm2-3A, do significantly alter it. Deletion of Pol32, a subunit of DNA Pol δ, significantly increases this distance. Mechanistically, Pol32 binds to histone H3-H4 and is critical for nucleosome assembly on the lagging strand. Together, we propose that Pol32 establishes a connection between nucleosome assembly and the processing of OFs on lagging strands.


Assuntos
DNA Polimerase III , DNA , Histonas , Nucleossomos , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae , Nucleossomos/metabolismo , Histonas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , DNA Polimerase III/metabolismo , DNA Polimerase III/genética , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/genética , DNA/metabolismo , Replicação do DNA , Ligação Proteica , DNA Polimerase Dirigida por DNA
12.
Int J Mol Sci ; 25(14)2024 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-39062956

RESUMO

Exosomes are small vesicles containing proteins, nucleic acids, and biological lipids, which are responsible for intercellular communication. Studies have shown that exosomes can be utilized as effective drug delivery vehicles to accurately deliver therapeutic substances to target tissues, enhancing therapeutic effects and reducing side effects. Mesenchymal stem cells (MSCs) are a class of stem cells widely used for tissue engineering, regenerative medicine, and immunotherapy. Exosomes derived from MSCs have special immunomodulatory functions, low immunogenicity, the ability to penetrate tumor tissues, and high yield, which are expected to be engineered into efficient drug delivery systems. Despite the promising promise of MSC-derived exosomes, exploring their optimal preparation methods, drug-loading modalities, and therapeutic potential remains challenging. Therefore, this article reviews the related characteristics, preparation methods, application, and potential risks of MSC-derived exosomes as drug delivery systems in order to find potential therapeutic breakthroughs.


Assuntos
Sistemas de Liberação de Medicamentos , Exossomos , Células-Tronco Mesenquimais , Exossomos/metabolismo , Humanos , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/citologia , Sistemas de Liberação de Medicamentos/métodos , Animais , Portadores de Fármacos/química , Neoplasias/terapia
13.
J Hazard Mater ; 476: 135132, 2024 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-39002483

RESUMO

The widespread presence of microplastics (MPs) in aquatic environments has become a significant concern, with freshwater sediments acting as terminal sinks, rapidly picking up these emerging anthropogenic particles. However, the accumulation, transport, degradation and biochemical impacts of MPs in freshwater sediments remain unresolved issues compared to other environmental compartments. Therefore, this paper systematically revealed the spatial distribution and characterization information of MPs in freshwater (rivers, lakes, and estuaries) sediments, in which small-size (<1 mm), fibers, transparent, polyethylene (PE), and polypropylene (PP) predominate, and the average abundance of MPs in river sediments displayed significant heterogeneity compared to other matrices. Next, the transport kinetics and drivers of MPs in sediments are summarized, MPs transport is controlled by the particle diversity and surrounding environmental variability, leading to different migration behaviors and transport efficiencies. Also emphasized the spatio-temporal evolution of MPs degradation processes and biodegradation mechanisms in sediments, different microorganisms can depolymerize high molecular weight polymers into low molecular weight biodegradation by-products via secreting hydrolytic enzymes or redox enzymes. Finally, discussed the ecological impacts of MPs on microbial-nutrient coupling in sediments, MPs can interfere with the ecological balance of microbially mediated nutrient cycling by altering community networks and structures, enzyme activities, and nutrient-related functional gene expressions. This work aims to elucidate the plasticity characteristics, fate processes, and potential ecological impact mechanisms of MPs in freshwater sediments, facilitating a better understanding of environmental risks of MPs in freshwater sediments.


Assuntos
Água Doce , Sedimentos Geológicos , Microplásticos , Poluentes Químicos da Água , Microplásticos/toxicidade , Sedimentos Geológicos/química , Poluentes Químicos da Água/análise , Água Doce/química , Monitoramento Ambiental , Biodegradação Ambiental
14.
J Hazard Mater ; 477: 135240, 2024 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-39079302

RESUMO

Despite extensive substitution of biodegradable plastics (BPs) for conventional plastics (CPs), research on their environmental ecological consequences as microplastics (MPs) is scarce. This study aimed to fill this gap by investigating the impacts of six prototypical MPs (categorized into BMPs and CMPs) on plant growth, cadmium (Cd) translocation, and bacterial communities in contaminated sediments. Results showed both BMPs and CMPs hindered plant development; yet interestingly, BMPs provoked more pronounced physiological and biochemical changes alongside increased oxidative stress due to reactive oxygen species accumulation. Notably, most MP types promoted the absorption of Cd by plant roots potentially via a "dilution effect". BMPs also induced larger shifts in soil microbial metabolic functions compared to CMPs. Ramlibacter was identified as a key biomarker distinguishing BMPs from CMPs, with link to multiple N metabolic pathways and N assimilation. This study offers novel insights into intricate biochemical mechanisms and environmental chemistry behaviors underpinning MP-Cd interactions within the plant-microbe-sediment system, emphasizing BMPs' higher potential ecological risks based on their significant effects on plant health and microbial ecology. This work contributes to enhancing the comprehensive understanding of their ecological implications and potential threats to environmental security.


Assuntos
Cádmio , Sedimentos Geológicos , Microplásticos , Microbiologia do Solo , Poluentes do Solo , Cádmio/metabolismo , Cádmio/toxicidade , Microplásticos/toxicidade , Microplásticos/metabolismo , Sedimentos Geológicos/microbiologia , Sedimentos Geológicos/química , Poluentes do Solo/metabolismo , Biodegradação Ambiental , Raízes de Plantas/metabolismo , Raízes de Plantas/microbiologia , Plásticos Biodegradáveis/metabolismo , Plantas/metabolismo , Desenvolvimento Vegetal/efeitos dos fármacos
15.
Food Microbiol ; 123: 104588, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39038893

RESUMO

Aspergillus flavus infects important crops and produces carcinogenic aflatoxins, posing a serious threat to food safety and human health. Biochemical analysis and RNA-seq were performed to investigate the effects and mechanisms of piperitone on A. flavus growth and aflatoxin B1 biosynthesis. Piperitone significantly inhibited the growth of A. flavus, AFB1 production, and its pathogenicity on peanuts and corn flour. Differentially expressed genes (DEGs) associated with the synthesis of chitin, glucan, and ergosterol were markedly down-regulated, and the ergosterol content was reduced, resulting in a disruption in the integrity of the cell wall and cell membrane. Moreover, antioxidant genes were down-regulated, the correspondingly activities of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase were reduced, and levels of superoxide anion and hydrogen peroxide were increased, leading to a burst of reactive oxygen species (ROS). Accompanied by ROS accumulation, DNA fragmentation and cell autophagy were observed, and 16 aflatoxin cluster genes were down-regulated. Overall, piperitone disrupts the integrity of the cell wall and cell membrane, triggers the accumulation of ROS, causes DNA fragmentation and cell autophagy, ultimately leading to defective growth and impaired AFB1 biosynthesis.


Assuntos
Aflatoxina B1 , Antifúngicos , Aspergillus flavus , Espécies Reativas de Oxigênio , Zea mays , Aspergillus flavus/efeitos dos fármacos , Aspergillus flavus/genética , Aspergillus flavus/crescimento & desenvolvimento , Aspergillus flavus/metabolismo , Zea mays/microbiologia , Antifúngicos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Arachis/microbiologia , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo
16.
J Mater Chem B ; 12(33): 8142-8152, 2024 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-39058226

RESUMO

Medical adhesives have been used for wound closure with many advantages over sutures, but the wet environment in the human body poses a big challenge for its application. The currently used dry double-sided tape (DST) can remove the water barrier by water absorption, but its over-swelling makes it difficult to achieve long-term adhesion. In this study, a dry double-sided tape post-treated with tannic acid (DST-TA) was developed. A double network adhesive composed of polyacrylic acid and gelatin was first prepared by free radical photocrosslinking, and was post-treated in acidic (pH = 2) tannic acid solution. Tannic acid was immobilized in the DST through the catecholyl group, which could form hydrogen bonds with the DST, or react with the amino group on the gelatin by oxidizing to quinone. In vivo and in vitro studies demonstrated that DST-TA had significantly higher swelling resistance and tensile strength than DST. The introduced catecholyl group could reduce over-swelling of the DST, and improve short-term and long-term adhesion in a wet environment. We also demonstrated that the DST-TA had good hemocompatibility, biodegradability, and no cytotoxicity, offering a potential option for long-term medical adhesive in a wet environment.


Assuntos
Gelatina , Polifenóis , Animais , Humanos , Resinas Acrílicas/química , Resinas Acrílicas/farmacologia , Gelatina/química , Teste de Materiais , Polifenóis/química , Polifenóis/farmacologia , Propriedades de Superfície , Resistência à Tração , Adesivos Teciduais/química , Adesivos Teciduais/farmacologia
17.
Toxins (Basel) ; 16(7)2024 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-39057925

RESUMO

Aspergillus flavus and its carcinogenic secondary metabolites, aflatoxins, not only cause serious losses in the agricultural economy, but also endanger human health. Rhein, a compound extracted from the Chinese herbal medicine Rheum palmatum L. (Dahuang), exhibits good anti-inflammatory, anti-tumor, and anti-oxidative effects. However, its effect and underlying mechanisms against Aspergillus flavus have not yet been fully illustrated. In this study, we characterized the inhibition effect of rhein on A. flavus mycelial growth, sporulation, and aflatoxin B1 (AFB1) biosynthesis and the potential mechanism using RNA-seq analysis. The results indicate that A. flavus mycelial growth and AFB1 biosynthesis were significantly inhibited by 50 µM rhein, with a 43.83% reduction in colony diameter and 87.2% reduction in AFB1 production. The RNA-seq findings demonstrated that the differentially expressed genes primarily participated in processes such as spore formation and development, the maintenance of cell wall and membrane integrity, management of oxidative stress, the regulation of the citric acid cycle, and the biosynthesis of aflatoxin. Biochemical verification experiments further confirmed that 50 µM rhein effectively disrupted cell wall and membrane integrity and caused mitochondrial dysfunction through disrupting energy metabolism pathways, leading to decreased ATP synthesis and ROS accumulation, resulting in impaired aflatoxin biosynthesis. In addition, a pathogenicity test showed that 50 µM rhein inhibited A. flavus spore growth in peanut and maize seeds by 34.1% and 90.4%, while AFB1 biosynthesis was inhibited by 60.52% and 99.43%, respectively. In conclusion, this research expands the knowledge regarding the antifungal activity of rhein and provides a new strategy to mitigate A. flavus contamination.


Assuntos
Aflatoxina B1 , Antraquinonas , Aspergillus flavus , Espécies Reativas de Oxigênio , Aspergillus flavus/efeitos dos fármacos , Aspergillus flavus/metabolismo , Aspergillus flavus/crescimento & desenvolvimento , Antraquinonas/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Aflatoxina B1/biossíntese , Aflatoxina B1/toxicidade , Metabolismo Energético/efeitos dos fármacos , Esporos Fúngicos/efeitos dos fármacos , Esporos Fúngicos/crescimento & desenvolvimento , Micélio/efeitos dos fármacos , Micélio/crescimento & desenvolvimento , Antifúngicos/farmacologia
18.
BMC Musculoskelet Disord ; 25(1): 597, 2024 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-39075431

RESUMO

OBJECTIVE: To investigate the biomechanical effects of percutaneous vertebroplasty combined with cement pedicle plasty (PVCPP) on the unstable osteoporotic vertebral fractures (OVFs) through finite element (FE) analysis. The study compares the biomechanical stability of finite element models between percutaneous vertebroplasty (PVP) and percutaneous vertebroplasty combined with cement pedicle plasty. METHODS: Two patients with unstable OVFs underwent computed tomography (CT) examination at the thoracolumbar vertebral body levels, respectively. The CT images were reconstructed into three-dimensional finite element models to simulate stress conditions across six dimensions and to evaluate the vertebral von Mises stress before and after bone cement reinforcement. RESULTS: The study found that stress distribution differed between groups mainly at the pedicle base. In the surgical vertebral bodies, the maximum stress in the PVP group decreased during flexion and left bending, while it increased in other states. In the PVCPP group, all maximum stresses decreased. In the inferior vertebral bodies, the maximum stress in the PVP group generally increased, while it decreased in the PVCPP group. In the superior vertebral bodies, postoperatively, the maximum stress in the PVP group generally increased, while it almost remained unchanged in the PVCPP group. PVP group had higher cement stress and displacement. CONCLUSION: PVCPP is an effective treatment method for patients with unstable OVFs. It can quickly relieve pain and enhance the stability of the three columns, thereby reducing the risk of some complications.


Assuntos
Cimentos Ósseos , Análise de Elementos Finitos , Vértebras Lombares , Fraturas por Osteoporose , Fraturas da Coluna Vertebral , Vertebroplastia , Humanos , Vertebroplastia/métodos , Fenômenos Biomecânicos/fisiologia , Fraturas da Coluna Vertebral/cirurgia , Fraturas da Coluna Vertebral/diagnóstico por imagem , Fraturas da Coluna Vertebral/fisiopatologia , Feminino , Vértebras Lombares/cirurgia , Vértebras Lombares/diagnóstico por imagem , Idoso , Fraturas por Osteoporose/cirurgia , Fraturas por Osteoporose/diagnóstico por imagem , Fraturas por Osteoporose/fisiopatologia , Vértebras Torácicas/cirurgia , Vértebras Torácicas/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Masculino , Estresse Mecânico , Idoso de 80 Anos ou mais
19.
Orthop Surg ; 16(8): 1963-1973, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38961670

RESUMO

OBJECTIVE: Currently, there is no established guideline on whether to opt for percutaneous endoscopic lumbar discectomy (PELD) or traditional transforaminal lumbar interbody fusion (TLIF) surgery based on specific types of lumbar disc herniation (LDH). Based on the Michigan State University (MSU) classification system, this study conducted a medium- to long-term follow-up analysis of two surgical methods over 5 years for the first time, aiming to provide empirical evidence to assist in making more informed decisions before surgery for LDH treatment. METHODS: This was a retrospective study that included 273 patients with single-level LDH who underwent PELD or TLIF treatment at our hospital between January 1, 2016, and December 31, 2018. Detailed metrics included preoperative and postoperative visual analogue scale (VAS) scores and Oswestry disability index (ODI) at 1-day, 1-week, 1-year, and 5-year follow-ups. Complications, recurrences, and 5-year postoperative modified MacNab criteria scores were also recorded. Statistical methods included independent sample t-tests, repeated measures analysis of variance (ANOVA), and χ2 tests. RESULTS: Classified into seven groups according to the MSU classification, it was found that there was an improvement in the VAS and ODI scores at four postoperative follow-ups (p < 0.001). PELD showed better results than TLIF in reducing pain and improving the ODI scores in the classifications of 3B, 2B, and 2C (p < 0.05). TLIF demonstrated consistent superiority over PELD in 2A, 2AB, 3A, and 3AB classifications (p < 0.05). The total recurrence rate in the PELD group (11.05%) within 5 years after surgery was higher (p < 0.05) than that in the TLIF group (3.96%). These were mainly concentrated in the 2A, 2AB, 3A, and 3AB types. Moreover, the rate of excellent and good outcomes in the PELD was higher than in the TLIF but no significant difference (χ2 = 1.0568, p = 0.5895). CONCLUSION: This study suggests that PELD and TLIF may relieve LDH, but have advantages under different MSU classifications. The MSU classification has specific guiding significance and could aid in the surgical selection of PELD or TLIF to achieve optimal treatment outcomes for patients with lumbar disc herniation.


Assuntos
Discotomia Percutânea , Endoscopia , Deslocamento do Disco Intervertebral , Vértebras Lombares , Fusão Vertebral , Humanos , Estudos Retrospectivos , Deslocamento do Disco Intervertebral/cirurgia , Deslocamento do Disco Intervertebral/classificação , Discotomia Percutânea/métodos , Masculino , Feminino , Vértebras Lombares/cirurgia , Pessoa de Meia-Idade , Fusão Vertebral/métodos , Adulto , Endoscopia/métodos , Medição da Dor , Avaliação da Deficiência , Idoso
20.
Complement Ther Med ; 84: 103068, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39004289

RESUMO

BACKGROUND: The utilization of Tuina as a therapeutic intervention for the management of chronic pain has experienced a gradually increase in its popularity, and the purpose of this bibliometric analysis is to offer a comprehensive understanding of the current state and frontier trends, as well as to provide recommendations for future research directions. METHODS: Publications on Tuina for chronic pain published between 2004 and 2023 were retrieved from the Web of Science Core Collection (WoSCC). Microsoft Excel, CiteSpace, VOSViewer, and the R package "bibliometrix" were used to quantitatively analyse the annual publication volume, countries/regions, journals, institutions, cited references, authors, and keywords. RESULTS: A total of 287 publications were retrieved. The number of annual publications on the use of Tuina for treating chronic pain has gradually increased. Most publications were published in China and the United States. Notably, the most productive institution and author were identified as Shanghai University of Traditional Chinese Medicine and Min Fang, respectively. Medicine ranked first as the most influential affiliate and most productive journal. These publications came from 1650 authors, among whom Edzard Ernst had the most co-citations. Keyword analysis revealed that the new research frontier was low back pain. CONCLUSION: The utilization of Tuina for the treatment of chronic pain has been gaining increasing recognition. Acupuncture, randomised controlled trials, systematic reviews, etc. were the main research subjects. Furthermore, low back pain is the new research frontier. This study provides an in-depth perspective on Tuina for chronic pain, which provides valuable reference material for clinicians with insights of therapeutic strategy, educators with valuable topics, and researchers with new research directions.


Assuntos
Bibliometria , Dor Crônica , Manejo da Dor , Humanos , Dor Crônica/terapia , Manejo da Dor/métodos , Medicina Tradicional Chinesa/métodos
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