Assuntos
Ergonomia/métodos , Doenças Musculoesqueléticas/reabilitação , Doenças Profissionais/reabilitação , Instituições de Assistência Ambulatorial , Terminais de Computador , Desenho de Equipamento , Seguimentos , Guias como Assunto , Humanos , Decoração de Interiores e Mobiliário , Minnesota/epidemiologia , Doenças Musculoesqueléticas/epidemiologia , Doenças Musculoesqueléticas/etiologia , Doenças Profissionais/epidemiologia , Doenças Profissionais/etiologia , Postura , Prevalência , Inquéritos e QuestionáriosRESUMO
We determined the complete nucleotide sequence of the coding region of the human peripherin gene (PRPH), as well as 742 bp 5' to the cap site and 584 bp 3' to the stop codon, and compared its structure and sequence to the rat and mouse genes. The overall structure of 9 exons separated by 8 introns is conserved among these three mammalian species. The nucleotide sequences of the human peripherin gene exons were 90% identical to the rat gene sequences, and the predicted human peripherin protein differed from rat peripherin at only 18 of 475 amino acid residues. Comparison of the 5' flanking regions of the human peripherin gene and rodent genes revealed extensive areas of high homology. Additional conserved segments were found in introns 1 and 2. Within the 5' region, potential regulatory sequences, including a nerve growth factor negative regulatory element, a Hox protein binding site, and a heat shock element, were identified in all peripherin genes. The positional conservation of each element suggests that they may be important in the tissue-specific, developmental-specific, and injury-specific expression of the peripherin gene.
Assuntos
Genes , Proteínas de Filamentos Intermediários/genética , Glicoproteínas de Membrana , Proteínas do Tecido Nervoso , Sequências Reguladoras de Ácido Nucleico , Sequência de Aminoácidos , Animais , Sequência de Bases , Regulação da Expressão Gênica , Humanos , Camundongos , Dados de Sequência Molecular , Periferinas , Ratos , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
Apolipoprotein J (apoJ), a glycoprotein associated with subclasses of plasma high density lipoproteins (HDL), was found to accumulate in aortic lesions in a human subject with transplantation-associated arteriosclerosis and in mice fed a high-fat atherogenic diet. Foam cells present in mouse aortic valve lesions expressed apoJ mRNA, suggesting local synthesis contributes to apoJ's localization in atherosclerotic plaque. As a prerequisite for elucidating the physiological function of apoJ by using a mouse model, cDNA clones representing the mouse homolog of apoJ were isolated, characterized, and sequenced. The nucleotide sequence predicts a 448 amino acid, 50,260 dalton protein. There was 81% nucleotide sequence similarity between mouse and human apoJ, and 75% similarity at the amino acid level. Mouse apoJ contains six potential N-glycosylation sites, a potential Arg-Ser cleavage site to generate alpha and beta subunits, a cluster of five cysteine residues in each subunit, three putative amphipathic helices, and four potential heparin-binding domains. Southern blot analysis indicates that the gene encompasses approximately 23 kb of DNA. Recombinant inbred strains were used to map apoJ to mouse chromosome 14, tightly linked to Mtv-11. All of the transcribed portions of the gene were cloned and analyzed, and all intron-exon boundaries were defined. The first of the 9 exons is untranslated. Single exons encode the signal peptide, the cysteine-rich domain in the alpha subunit, two potential amphipathic helices flanking a heparin-binding consensus sequence, and a potential amphipathic helix overlapping a heparin-binding domain, supporting their potential functional significance in apoJ. A variety of mouse tissues constitutively express a 1.9 kb apoJ mRNA, with apparently identical transcriptional start sites utilized in all tissues tested. ApoJ mRNA was most abundant in stomach, liver, brain, and testis, with intermediate levels in heart, ovary, and kidney. The high degree of similarity between mouse and human apoJ, in structure and distribution of the gene product, gene structure, and deposition in atherosclerotic plaques, suggests that the mouse is an ideal model with which to elucidate the role of apoJ in HDL metabolism and atherogenesis.
Assuntos
Arteriosclerose/genética , Glicoproteínas/genética , Chaperonas Moleculares , Sequência de Aminoácidos , Animais , Arteriosclerose/diagnóstico , Sequência de Bases , Southern Blotting , Mapeamento Cromossômico , Clusterina , Vasos Coronários/patologia , DNA Complementar/análise , Modelos Animais de Doenças , Marcadores Genéticos , Genoma , Glicoproteínas/análise , Humanos , Immunoblotting , Imuno-Histoquímica , Hibridização In Situ , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Dados de Sequência Molecular , RNA Mensageiro/análiseRESUMO
Mammalian neurons and neuron-like cultured cells express the neural intermediate filament (IF) proteins neurofilament (NF)-L, NF-M, NF-H, and peripherin. To determine whether these proteins are found within the same 10-nm filament, light and electron microscope immunocytochemistry using peripherin and NF-specific antibodies was performed on PC12 cells, nervous tissue, and isolated neural filaments from the cauda equina. Double-label immunofluorescence showed that peripherin and NF-L, -M, and -H were found in identical filamentous patterns in interphase and mitotic PC12 cells. Furthermore, expression of mutant peripherin in PC12 cells disrupted not only the peripherin network but also NF-containing filaments. Immunoelectron microscopy of PC12 cell cytoskeletons showed that peripherin and NF subunit proteins were found in the same filament. In situ, in the sciatic nerve, peripherin/NF-L or peripherin/NF-M/-H double-label immunofluorescence illustrates at least three types of nerve fibers: those containing NF only, those labeled predominantly for peripherin, and fibers in which peripherin and NF subunits were colocalized. Immunoelectron microscopy of filaments isolated from nerve roots comprising the sciatic nerve also showed the same three labeling patterns seen by light microscopy. Some neural IF appear to contain predominantly NF proteins or peripherin, but in others, both proteins are found within the same IF.
Assuntos
Proteínas de Filamentos Intermediários/análise , Filamentos Intermediários/ultraestrutura , Glicoproteínas de Membrana , Proteínas do Tecido Nervoso , Proteínas de Neurofilamentos/análise , Sequência de Aminoácidos , Animais , Anticorpos , Anticorpos Monoclonais , Encéfalo/fisiologia , Deleção Cromossômica , Códon , Imunofluorescência , Genes myc , Biblioteca Genômica , Imuno-Histoquímica , Proteínas de Filamentos Intermediários/genética , Filamentos Intermediários/fisiologia , Microscopia Imunoeletrônica , Dados de Sequência Molecular , Proteínas de Neurofilamentos/genética , Neuropeptídeos/análise , Células PC12 , Periferinas , Mapeamento por Restrição , Nervo Isquiático/citologia , TransfecçãoRESUMO
In order to determine the CNS distribution of the 57 kDa neural intermediate filament protein (NIFP), a specific antiserum was used in immunofluorescence studies on serial sections taken from each spinal cord level and at 300-microns intervals through the rat brain. The labeling pattern was recorded onto camera lucida tracings of adjacent sections stained with hematoxylin and eosin/luxol fast blue. Three major immunolocalization patterns were revealed. (1) Both large-caliber and fine-caliber axons of optic and all brain stem cranial nerves and their tracts except for the auditory portion of the VIIIth nerve. (2) An extensive array of fine-caliber fibers in the cerebellar white matter and brain stem with region-specific variation in pattern and density. Prominent among the regions with dense arrays of labeled processes were selective cerebellar afferent systems, particularly olivocerebellar fibers, visual afferents arising in the retina, and selective regions of reticular formation. In contrast to the brain stem, the telencephalon contained rare label. (3) Filamentous labeling of neuronal cytokarya in sensory ganglia and a small number of CNS nuclear groups, including all autonomic nuclei and the cholinergic pontine nuclei. On sections of selected CNS regions, distribution patterns of the 57 kDa NIFP were correlated to results obtained by in situ hybridization of a cDNA probe for the 57 kDa NIFP. The data suggest the possible existence of neuronal IF proteins specifically related to selective anatomic and/or neurotransmitter systems.
Assuntos
Sistema Nervoso Central/metabolismo , Proteínas de Filamentos Intermediários/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Animais , Axônios/metabolismo , Sistema Nervoso Central/citologia , Sistema Nervoso Central/ultraestrutura , Neurônios/metabolismo , Ratos , Distribuição TecidualRESUMO
A cDNA (199E) specific for the 57 kd neural IF protein has been isolated from a PC12 cell lambda gt11 library. Antibody eluted from the fusion protein produced by 199E recognizes the 57 kd protein on immunoblots and, in PC12 cells, labels a pattern of fibrillar structures identical to that seen with 57 kd antiserum. In situ hybridization using antisense RNA transcripts labels areas of the nervous system known to contain the 57 kd protein. 199E hybridizes with a single mRNA species of approximately 2.0 kb from PC12 cells. A 199E-reactive message can be detected as early as E10 in rat embryos. Southern analyses suggest that there is only one gene for this protein. Amino acid sequence predicted from 199E indicates that the 57 kd protein is a type III IF protein like vimentin and desmin. Thus, expression of IF structural genes in neurons is not limited to the type IV neuronal IF triplet proteins.