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1.
PeerJ Comput Sci ; 10: e2193, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39145247

RESUMO

The combination of memory forensics and deep learning for malware detection has achieved certain progress, but most existing methods convert process dump to images for classification, which is still based on process byte feature classification. After the malware is loaded into memory, the original byte features will change. Compared with byte features, function call features can represent the behaviors of malware more robustly. Therefore, this article proposes the ProcGCN model, a deep learning model based on DGCNN (Deep Graph Convolutional Neural Network), to detect malicious processes in memory images. First, the process dump is extracted from the whole system memory image; then, the Function Call Graph (FCG) of the process is extracted, and feature vectors for the function node in the FCG are generated based on the word bag model; finally, the FCG is input to the ProcGCN model for classification and detection. Using a public dataset for experiments, the ProcGCN model achieved an accuracy of 98.44% and an F1 score of 0.9828. It shows a better result than the existing deep learning methods based on static features, and its detection speed is faster, which demonstrates the effectiveness of the method based on function call features and graph representation learning in memory forensics.

2.
Artigo em Inglês | MEDLINE | ID: mdl-39172366

RESUMO

A basic function of human visual perception is the ability to recognize and locate objects in the environment. It has been shown that two-dimensional (2D) location can reliably bias judgments on object identity (spatial congruency bias; Golomb et al., 2014), suggesting that 2D location information is automatically bound with object features to induce such a bias. Although the binding problem of feature and location has been vigorously studied under various 2D settings, it remains unclear how depth location can be bound with object features in a three-dimensional (3D) setting. Here we conducted five experiments in various 3D contexts using the congruency bias paradigm, and found that changes of object's depth location could influence perceptual judgments on object features differently depending on whether its relative depth order with respect to others changed or not. Experiments 1 and 2 showed that the judgments on an object's color could be affected by changes in its ordinal depth, but not by changes in its absolute metric depth. Experiment 3 showed that the bias was asymmetric-changes in an object's color did bias the judgments on metric-depth location, but not if its depth order had changed. Experiments 4 and 5 investigated whether these findings could be generalized to a peripersonal near space and a large-scale far space, respectively, using more ecological virtual environments. Our findings suggest that ordinal depth plays a special role in feature-location binding: an object may be automatically bound with its relative depth relation, but not with its absolute metric-depth location. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

3.
BMC Musculoskelet Disord ; 25(1): 685, 2024 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-39217321

RESUMO

BACKGROUND: Simultaneous bilateral total hip arthroplasty (SI-THA) results in more blood loss and a greater need for postoperative allogeneic blood transfusion (ABT). Previous studies have reported that multimodal patient blood management (PBM) strategies were associated with a smaller effect of intraoperative cell salvage (ICS) in unilateral total hip arthroplasty. However, there are few studies on the role of ICS in SI-THA. This study aims to explore the effect of ICS with multimodal PBM strategies on SI-THA and to identify risk factors associated with ABT. METHODS: This retrospective matched cohort study included 72 patients in the ICS group and 72 patients in the control group who were matched according to age, sex, and year of hospitalization. Demographic data, hematological indicators, blood loss, and ABT were compared between the two groups. Logistic regression analysis was performed to identify independent risk factors for postoperative ABT. Postoperative outcomes were also recorded. RESULTS: In the cohort of 144 patients, 27 patients (37.5%) in the ICS group while 45 patients (62.5%) in the control group received postoperative ABT after SI-THA. Compared with the control group, the ICS group showed significant differences in terms of blood loss, postoperative hemoglobin and hematocrit. The transfused volume of allogeneic red blood cells per ABT patient was also lower in the ICS group. Multivariate logistic regression analysis indicated that sex, the utilization of ICS, and preoperative hematocrit level were identified as independent factors associated with postoperative ABT. The utilization of ICS significantly shortened off-bed time and length of hospital stay, but had no effect on early pain and functional outcomes. CONCLUSION: The utilization of ICS can significantly affect postoperative ABT in SI-THA patients with multimodal PBM strategies. Sex, the utilization of ICS and preoperative hematocrit level were identified as independent factors associated with postoperative ABT. The utilization of ICS promoted weight-bearing functional exercises, but had no effect on early outcomes.


Assuntos
Artroplastia de Quadril , Recuperação de Sangue Operatório , Humanos , Masculino , Artroplastia de Quadril/efeitos adversos , Artroplastia de Quadril/métodos , Feminino , Estudos Retrospectivos , Pessoa de Meia-Idade , Idoso , Recuperação de Sangue Operatório/métodos , Perda Sanguínea Cirúrgica/prevenção & controle , Perda Sanguínea Cirúrgica/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Fatores de Risco , Transfusão de Sangue/estatística & dados numéricos , Resultado do Tratamento , Transfusão de Sangue Autóloga/métodos
4.
BMC Musculoskelet Disord ; 25(1): 370, 2024 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-38730370

RESUMO

BACKGROUND: In this study, we present the unique case of a patient with knee osteoarthritis (OA) of the medial compartment and posterior cruciate ligament (PCL) deficiency who underwent simultaneous medial unicompartmental knee arthroplasty (UKA) and PCL reconstruction. CASE PRESENTATION: A 49-year-old male patient presented with a 1-year history of pain and instability in the left knee. The patient had previously experienced a trauma-related injury to the PCL of the left knee that was left untreated. Imaging and physical examination confirmed the presence of left medial knee OA along with PCL rupture. To address these issues, the patient underwent UKA combined with PCL reconstruction. The patient's Lysholm score was 47 before surgery and 81 three months after surgery, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score was 29 before surgery and 18 three months after surgery, and the International Knee Documentation Committee (IKDC) subjective score was 56.3 before surgery and 74.7 three months after surgery. Six months after surgery, the patient's gait returned to normal, and he was able to jog. CONCLUSION: This case report presents the first instance of UKA combined with PCL reconstruction and introduces a novel treatment approach for patients suffering from medial knee OA and ligament injury.


Assuntos
Artroplastia do Joelho , Osteoartrite do Joelho , Reconstrução do Ligamento Cruzado Posterior , Ligamento Cruzado Posterior , Humanos , Masculino , Pessoa de Meia-Idade , Artroplastia do Joelho/métodos , Osteoartrite do Joelho/cirurgia , Reconstrução do Ligamento Cruzado Posterior/métodos , Ligamento Cruzado Posterior/cirurgia , Ligamento Cruzado Posterior/lesões , Resultado do Tratamento , Articulação do Joelho/cirurgia , Articulação do Joelho/diagnóstico por imagem
5.
Org Biomol Chem ; 22(17): 3439-3443, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38591416

RESUMO

This study reports a metal- and light-free decarboxylative C-H alkylation of heteroarenes at room temperature. The reaction generates various primary, secondary, and tertiary alkyl radicals and functionalizes seven different privileged scaffolds widely present in bioactive molecules. During this process, one equivalent of hypervalent iodine(III) carboxylates (HICs) plays dual roles as an alkyl radical precursor and an oxidant.

6.
Orthop Surg ; 16(4): 873-881, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38384178

RESUMO

OBJECTIVES: Despite the use of multimodal analgesia, patients undergoing knee arthroplasty still encounter residual moderate pain. The addition of betamethasone to local anesthetic has been shown to improve postoperative pain. However, it remains uncertain whether the positive effects of perineural or intravenous administration of betamethasone on analgesia outcomes lead to better early mobility and postoperative recovery. METHODS: Between June 2022 and February 2023, a total of 159 patients who were undergoing knee arthroplasty were included in this study. These patients were allocated randomly into three groups: (i) the NS group, received ropivacaine 0.375% and intravenous 3mL 0.9% normal saline; (ii) the PNB group, received ropivacaine 0.375% plus perineural betamethasone (12mg) 3mL and intravenous 3mL 0.9% normal saline; and (iii) the IVB group, received ropivacaine 0.375% and intravenous betamethasone (12mg) 3mL. RESULTS: Both perineural and intravenous administration of betamethasone led to improved median (IQR) numeric rating scale (NRS) scores on the 6-meter walk test, with a score of 1.0 (1.0-2.0) for both groups, compared with 2.0 (1.0-2.0) for the NS group (p = 0.003). Compared to the NS group, both the PNB and IVB groups showed significant reductions in NRS scores at 24 and 36 h after surgery, along with a significant increase in ROM at 24, 36, and 48 h post-operation. Additionally, it exhibited lower levels of cytokine IL-1ß and TNF-α in fluid samples, as well as lower level of HS-CRP in blood samples in the PNB and IVB groups compared to the NS group. CONCLUSION: The administration of perineural and intravenous betamethasone demonstrated an enhanced analgesic effect following knee arthroplasty. Furthermore, it was associated with reduced levels of IL-1ß, TNF-α, and HS-CRP, as well as enhanced knee ROM, which is conducive to early ambulation and postoperative rehabilitation after knee arthroplasty.


Assuntos
Artroplastia do Joelho , Betametasona , Nervo Femoral , Bloqueio Nervoso , Ropivacaina , Humanos , Administração Intravenosa , Amidas/efeitos adversos , Anestésicos Locais/administração & dosagem , Artroplastia do Joelho/efeitos adversos , Proteína C-Reativa/efeitos dos fármacos , Proteína C-Reativa/metabolismo , Método Duplo-Cego , Nervo Femoral/efeitos dos fármacos , Dor Pós-Operatória/tratamento farmacológico , Dor Pós-Operatória/prevenção & controle , Ropivacaina/administração & dosagem , Solução Salina/farmacologia , Solução Salina/uso terapêutico , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/efeitos dos fármacos , Betametasona/administração & dosagem , Interleucina-1beta/sangue , Interleucina-1beta/efeitos dos fármacos
7.
Orthop Surg ; 15(6): 1645-1653, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37092381

RESUMO

OBJECTIVE: Bone cement releases a large amount of heat as it polymerizes. Skin burns caused by discarded bone cement are not well understood during arthroplasty. It is important to study the correlates and mechanisms of scalding and to accurately evaluate the severity of burns to guide treatment decisions. METHODS: Standardized burns were created in eight anesthetized rabbits using different thicknesses of bone cement. Bone cement was uniformly stirred to make thicknesses of 1 mm, 4 mm, 8 mm, 12 mm, 16 mm, and 20 mm and a 20 × 40 mm cuboid. Bone cement samples were then placed on the back of a rabbit, and the temperature changes were recorded with an industrial digital thermometer. One hour later, the appearance of scalded skin was observed, and the rabbits were euthanized. The scalded parts were cut to make pathological sections and stained with HE, and the differences in the depth of the scalded skin caused by different thicknesses of bone cement were observed under a light microscope. RESULTS: Damage caused by 1 mm-, 4 mm-, 8 mm-, 12 mm-, 16 mm-, and 20 mm-thick bone cement samples mainly involved the epidermis, the papillary dermis, the reticular dermis layer, and the full thickness of the skin and the subcutaneous tissue. The maximum temperature of 1 mm, 4 mm, 8 mm, and 12 mm bone cementation had a statistically significant difference (p < 0.001), while there was no significant difference between 12 mm, 16 mm, and 20 mm samples (p = 0.856). The time to severe scalding with bone cement at temperatures above 70°C was significantly different between different thicknesses (p < 0.001). CONCLUSION: The heat released by different thicknesses of bone cement leads to different maximum temperatures and the duration of severe burns, resulting in different degrees of skin burns. Attention should be paid to discarded bone cement to prevent this potential complication in knee arthroplasty.


Assuntos
Artroplastia do Joelho , Queimaduras , Animais , Coelhos , Cimentos Ósseos , Pele , Temperatura Alta , Queimaduras/etiologia , Queimaduras/patologia
8.
J Asian Nat Prod Res ; 25(4): 369-378, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35763370

RESUMO

Erysolin and its two metabolites which were found in blood, ERY-GSH and ERY-NAC, were synthesized by alkylation, amination, isothiocyanation and oxidation reactions from 1-bromo-4-chlorobutane and sodium methyl mercaptide. The reaction temperature, time, feed ratios and purification method were also optimized. The synthesis method was simple, green, safe and low-cost. Erysolin, ERY-GSH and ERY-NAC showed good antitumor activities against MCF-7, HeLa, HepG2, A549 and SW480 cells, which suggested that the antitumor mechanism of erysolin can also be clarified from its metabolites in addition to itself.


Assuntos
Antineoplásicos , Tiocianatos , Humanos , Tiocianatos/farmacologia , Células HeLa , Sulfonas/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Antineoplásicos/farmacologia , Proliferação de Células
9.
J Pharm Anal ; 12(2): 270-277, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35582404

RESUMO

A fast, reliable, and cost-effective liquid chromatography-tandem mass spectrometry method was established to determine the effects of the traditional Chinese medicine employed to treat coronavirus disease 2019, namely, Lianhua Qingwen granules, Huoxiang Zhengqi capsules, Jinhua Qinggan granules, Shufeng Jiedu capsules, and Angong Niuhuang pills, on the pharmacokinetics of lopinavir/ritonavir in rats. Blood samples were prepared using the protein precipitation method and atazanavir was selected as the internal standard (IS). Separation was performed on an Agilent ZORBAX eclipse plus C18 (2.1 mm × 50 mm, 1.8 µm) column using acetonitrile and water containing 0.1% formic acid as the mobile phase for gradient elution. The flow rate was 0.4 mL/min and the injection volume was 2 µL. Agilent Jet Stream electrospray ionization was used for mass spectrometry detection under positive ion multiple reaction monitoring mode at a transition of m/z 629.3→447.3 for lopinavir, m/z 721.3→296.1 for ritonavir, and m/z 705.4→168.1 for the IS. The method showed good linearity in the concentration range of 25-2500 ng/mL (r=0.9981) for lopinavir and 5-500 ng/mL (r=0.9984) for ritonavir. The intra-day and inter-day precision and accuracy were both within ±15%. Items, such as dilution reliability and residual effect, were also within the acceptable limits. The method was used to determine the effects of five types of traditional Chinese medicines on the pharmacokinetics of lopinavir/ritonavir in rats. The pharmacokinetic results showed that the half-life of ritonavir in the groups administered Lianhua Qingwen granules and Huoxiang Zhengqi capsules combined with lopinavir/ritonavir was prolonged by approximately 1.5- to 2-fold relative to that in the control group. Similarly, the pharmacokinetic parameters of lopinavir were altered. Overall, the results of this study offer important theoretical parameters for the effective clinical use of five types of traditional Chinese medicines combined with lopinavir/ritonavir to reduce the occurrence of clinical adverse reactions.

10.
Cytotechnology ; 74(2): 217-229, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35464170

RESUMO

PBX1 expression has been found to be significantly reduced in nigrostriatal neurons of PD patients, but the effect of PBX1 on ROS and apoptosis in nigrostriatal dopamine neurons is not yet known. This paper aimed to explore whether PBX1 could be involved in the development of PD. The construction of the in vitro PD model was followed by the determination of PBX1 expression. Then, PBX1 was overexpressed to observe the changes in the cell viability, TH expression, oxidative stress and apoptosis of the model. The mitochondrial membrane potential analysis and detection of PINK1/parkin expression were also conducted. To observe whether FOXA1 was involved in the mechanism, its expression was measured, and its association with PBX1 was determined. Subsequently, FOXA1 was silenced to observe whether PBX1 did effects on dopaminergic neuron via FOXA1. PBX1 attenuates 6-OHDA-induced dopaminergic neuronal cell injury and oxidative stress, and apoptosis. Its overexpression ameliorates mitochondrial dysfunction in dopaminergic neurons and upregulates the expression of PINK1/parkin. PBX1 could combine with FOXA1 and affects 6-OHDA-induced dopaminergic neuronal damage and regulates PINK1/PARKIN expression via FOXA1. To conclude, PBX1 attenuates 6-OHDA-induced oxidative stress and apoptosis in dopaminergic neurons, and affected PINK1/PARKIN expression via FOXA1, which indicates the great potential of the PBX1 in prevention against PD.

11.
BMJ Open ; 12(4): e056025, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-35428633

RESUMO

INTRODUCTION: Mechanical thrombectomy (MT) had been regarded as the first-line therapy for acute ischaemic stroke patients. The sex differences in post-MT treatment outcomes were analysed by randomised controlled trial (RCT) studies with inconsistent conclusions. We suggest the results from the real-world data may differ from RCT containing studies. Therefore, the sex difference in non-clinical trial populations needs to be clarified. METHODS AND ANALYSIS: In order to obtain relative studies comprehensively, we will search the main document databases, consisting of Web of Science, Medline in Ovid, Embase in Ovid and Cochrane Library, and trials registers, including Clinical Trails register. The clinical outcomes of real-world studies published between January 2015 and March 2022 will be included. The assessment methods of bias risk will be performed according to study type. The inclusion of studies, evaluation of risk and publication bias, data extraction will be implemented by two reviewers, respectively. The primary outcomes include successful recanalisation and 90-day favourable outcome. Secondary outcomes include vascular complication, hospital-related complications, death during hospital stay and follow-up, and intracerebral haemorrhage. The risk bias of observational studies will be evaluated by Newcastle-Ottawa Scale. I2 statistic will be used to perform the assessment of study heterogeneity. ETHICS AND DISSEMINATION: With no need of ethics approval in this review, results in this review ground on public data. The results of the study will be eventually presented at international conferences or in a related journal. PROSPERO REGISTRATION NUMBER: CRD42021242597.


Assuntos
AVC Isquêmico , Acidente Vascular Cerebral , Humanos , AVC Isquêmico/cirurgia , Metanálise como Assunto , Caracteres Sexuais , Acidente Vascular Cerebral/tratamento farmacológico , Acidente Vascular Cerebral/cirurgia , Revisões Sistemáticas como Assunto , Trombectomia
12.
Biomed Res Int ; 2022: 8759060, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35211626

RESUMO

3D printing, also known as additive manufacturing, is a technology that uses a variety of adhesive materials such as powdered metal or plastic to construct objects based on digital models. Recently, 3D printing technology has been combined with digital medicine, materials science, cytology, and other multidisciplinary fields, especially in the field of orthopedic built-in objects. The development of advanced 3D printing materials continues to meet the needs of clinical precision medicine and customize the most suitable prosthesis for everyone to improve service life and satisfaction. This article introduces the development of 3D printing technology and different types of materials. We also discuss the shortcomings of 3D printing technology and the current challenges, including the poor bionics of 3D printing products, lack of ideal bioinks, product safety, and lack of market supervision. We also prospect the future development trends of 3D printing.


Assuntos
Ortopedia , Impressão Tridimensional , Próteses e Implantes , Previsões , Humanos , Desenho de Prótese
14.
Artigo em Inglês | MEDLINE | ID: mdl-34444545

RESUMO

The Chinese government has made great efforts to improve the scale efficiency of land through various measures during recent years, hoping to realize the coordinated developing goal of promoting agricultural benefits and protecting the environment. Statistics show that China's land scale efficiency has steadily increased, but agricultural nonpoint source (NPS) pollution has also increased, which seems contrary to the expected outcome. Can increasing scale efficiency really curb agricultural NPS pollution? This study uses provincial-level data from China, together with a panel model and spatial econometric model, to investigate the relationship between scale efficiency and agricultural NPS pollution. It is found that the increase of scale efficiency aggravates the agricultural NPS pollution, and the conclusion still holds after considering spatial effect. The results of spatial analysis shows that the agricultural NPS pollution is spatially dependent. Further decomposition of the spatial effect shows that the scale efficiency not only intensifies the local agricultural NPS pollution, but also has a spillover effect (though not statistically significant) on agricultural NPS pollution in the surrounding areas. It is worth noting that financial policy, raising wage income and upgrading industrial structure can effectively curb agricultural NPS pollution in this region and adjacent areas, which also deserves our attention in the control of agricultural NPS pollution. In addition, it is necessary to make financial and fiscal support policies specifically for the governance of agricultural NPS pollution, adjust the distorted prices of input factors such as chemicals and pesticide, and accelerate the transformation of small-sized farmers to family farms, in order to maximize the inhibitory effect of scale efficiency on relieving agricultural NPS pollution.


Assuntos
Poluição Difusa , Agricultura , China , Eficiência , Fazendeiros , Humanos , Análise Espacial
15.
Pharm Biol ; 59(1): 912-921, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34236293

RESUMO

CONTEXT: Valeriana jatamansi Jones [syn. V. wallichii DC, (Valerianaceae)] (VJJ) is used to treat depression. OBJECTIVE: To explore the effects of total iridoids of VJJ extract (TIV) on chronic unpredictable mild stress (CUMS) in mice. MATERIALS AND METHODS: VJJ roots and rhizomes were extracted with 70% ethanol. CUMS rats were treated daily with fluoxetine (2.6 mg/kg, i.g.) or TIV (5.7, 11.4, and 22.8 mg/kg, i.g.) for 14 days. Male Kun Ming mice on normal chow and 0.5% CMC-Na solution were used as a control. Behavioural tests included the tail suspension (TST) and sucrose preference tests (SPT). Evans blue staining was used to evaluate blood-brain barrier (BBB) permeability. Western blotting was used to measure zonula occludens-1 (ZO-1) and occludin expression. 16S rRNA sequencing was used to analyse intestinal flora abundance. Tax4Fun was used to predict KEGG metabolic pathways. RESULTS: TIV treatment reduced TST time (117.35 ± 8.23 or 108.95 ± 6.76 vs. 144.45 ± 10.30 s), increased SPT (55.83 ± 7.24 or 53.12 ± 13.85 vs. 38.98 ± 5.43%), increased the abundance of phylum Firmicutes (86.99 ± 0.03 vs. 60.88 ± 0.19%) and genus Lactobacillus (75.20 ± 0.19 vs. 62.10 ± 0.13%), reduced the abundance of phylum Bacteroidetes (6.69 ± 0.06 or 11.50 ± 0.09 vs. 25.07 ± 0.20%). TIV increased carbohydrate metabolism (14.50 ± 3.00 × 10-3 or 14.60 ± 2.00 × 10-3 or 14.90 ± 2.00 × 10-3 vs.13.80 ± 4.00 × 10-3%), replication and repair functions (5.60 ± 1.00 × 10-3 or 5.60 ± 1.00 × 10-3 vs. 5.10 ± 4.00 × 10-3%), reduced the frequency of infectious disease (1.60 ± 2.00 × 10-4 or 1.90 ± 5.00 × 10-4 or 1.80 ± 3.00 × 10-4 vs. 2.20 ± 7.00 × 10-3%), BBB permeability (0.77 ± 0.30 vs. 1.81 ± 0.33 µg/g), and up-regulated the expression of ZO-1 (1.42-fold, 1.60-fold, 1.71-fold) and occludin (1.79-fold, 2.20-fold). CONCLUSIONS: TIV may modulate the intestinal flora, thereby inducing the expression of ZO-1 and occludin, protecting the BBB and exerting an antidepressant effect.


Assuntos
Antidepressivos/farmacologia , Iridoides/farmacologia , Extratos Vegetais/farmacologia , Estresse Psicológico/tratamento farmacológico , Animais , Animais não Endogâmicos , Antidepressivos/administração & dosagem , Antidepressivos/isolamento & purificação , Barreira Hematoencefálica/metabolismo , Depressão/tratamento farmacológico , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Fluoxetina/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Iridoides/administração & dosagem , Iridoides/isolamento & purificação , Masculino , Camundongos , Ocludina/genética , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Ratos , Regulação para Cima/efeitos dos fármacos , Valeriana/química , Proteína da Zônula de Oclusão-1/genética
16.
RSC Adv ; 11(51): 32459-32463, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-35495530

RESUMO

A simple-structured super gelator with self-healability and multi-stimuli responses was reported herein, which exhibited multiple visual molecular recognition abilities. Multifunctional applications such as effective lubricants, safe fuels, high-efficient propellants, dyes adsorbents, enhanced fluorescence emission and separation of aldehydes from aqueous solutions are integrated into a single organogelator, which was rarely reported.

18.
ACS Nano ; 14(11): 14831-14845, 2020 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-33084319

RESUMO

DNA alkylating agents generally kill tumor cells by covalently binding with DNA to form interstrand or intrastrand cross-links. However, in the case of cisplatin, only a few DNA adducts (<1%) are highly toxic irreparable interstrand cross-links. Furthermore, cisplatin is rapidly detoxified by high levels of intracellular thiols such as glutathione (GSH). Since the discovery of its mechanism of action, people have been looking for ways to directly and efficiently remove intracellular GSH and increase interstrand cross-links to improve drug efficacy and overcome resistance, but there has been little breakthrough. Herein, we hypothesized that the anticancer efficiency of cisplatin can be enhanced through iodo-thiol click chemistry mediated GSH depletion and increased formation of DNA interstrand cross-links via mild hyperthermia triggered by near-infrared (NIR) light. This was achieved by preparing an amphiphilic polymer with platinum(IV) (Pt(IV)) prodrugs and pendant iodine atoms (iodides). The polymer was further used to encapsulate IR780 and assembled into Pt-I-IR780 nanoparticles. Induction of mild hyperthermia (43 °C) at the tumor site by NIR light irradiation had three effects: (1) it accelerated the GSH-mediated reduction of Pt(IV) in the polymer main chain to platinum(II) (Pt(II)); (2) it boosted the iodo-thiol substitution click reaction between GSH and iodide, thereby attenuating the GSH-mediated detoxification of cisplatin; (3) it increased the proportion of highly toxic and irreparable Pt-DNA interstrand cross-links. Therefore, we find that mild hyperthermia induced via NIR irradiation can enhance the killing of cancer cells and reduce the tumor burden, thus delivering efficient chemotherapy.


Assuntos
Antineoplásicos , Cisplatino , Reagentes de Ligações Cruzadas , Adutos de DNA , Glutationa , Hipertermia Induzida , Antineoplásicos/farmacologia , Cisplatino/farmacologia , DNA/genética , Humanos
19.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1106-1107: 35-42, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30639948

RESUMO

Qiangshen tablet, an important prescription consisting of 14 kinds of Chinese herbal medicines, has been used for decades to treat kidney yang deficiency syndrome (KYDS) in China. Qiangshen tablet has been recorded in ChP (2015 edition) and possesses the effect of strengthening yang, invigorating qi and tonifying kidneys. In this research, a simple, reliable and specific method was established for simultaneous determination of stachydrine, psoralen, isopsoralen, morroniside, paeoniflorin and loganin in normal and KYDS rat plasma after intragastric administration of a Qiangshen tablet suspension by UPLC-MS/MS. Protein precipitation (PP) by acetonitrile and liquid-liquid extraction (LLE) by ethyl acetate - n-butanol (1: 1, v/v) were used for pretreatment of plasma samples. Chromatographic separation of two IS (Internal Standard) and six analytes was achieved using an ACQUITY UPLC® BEH C18 column (2.1 × 100 mm, 1.7 µm). The mobile phase consisted of 0.1% formic acid aqueous solution (solvent A) and acetonitrile (solvent B) with a gradient scheme. Multiple reaction monitoring (MRM) mode with positive and negative ion source switching was applied to perform the mass spectrometric analyses. This method has been validated with good linearity (r ≥ 0.9942) and acceptable precision and accuracy (RSD ≤ 11%, RE from -4.8% to 7.7%). The mean recovery values of the analytes and IS were all ≥68.28%, and the matrix effects ranged from 94.4% to 101.7%. The stability of the IS and analytes was measured throughout the experiment. The results showed significant differences between the pharmacokinetic traits of the analytes in the normal and KYDS groups, suggesting that pharmacokinetic procedures involving these analytes could be modified in cases of KYDS.


Assuntos
Monitoramento de Medicamentos/métodos , Medicamentos de Ervas Chinesas , Deficiência da Energia Yang/tratamento farmacológico , Animais , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/farmacocinética , Ficusina/sangue , Furocumarinas/sangue , Glucosídeos/sangue , Glicosídeos/sangue , Iridoides/sangue , Masculino , Metaboloma , Monoterpenos/sangue , Prolina/análogos & derivados , Prolina/sangue , Ratos , Ratos Sprague-Dawley , Comprimidos/farmacocinética , Espectrometria de Massas em Tandem
20.
Planta Med ; 84(17): 1292-1299, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29925100

RESUMO

Six new neo-clerodane diterpenoids (1: -6: ), scutebatas X - Z, A1-C1, along with twelve known ones (7: -18: ) were obtained via the phytochemical investigation of the aerial parts of Scutellaria barbata. Their structures were established by detailed spectroscopic analysis. The absolute configurations of 1: and 2: , as the representative members of this type, were identified based on a circular dichroic exciton chirality method. Moreover, in vitro cytotoxicity of compounds 1: -6: were evaluated against three human cancer cell lines (SGC-7901, MCF-7, and A-549) using the MTT method. Compound 6: showed cytotoxic activities against all the three cell lines with IC50 values of 17.9, 29.9, and 35.7 µM, respectively.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Citotoxinas/farmacologia , Diterpenos Clerodânicos/farmacologia , Extratos Vegetais/química , Scutellaria/química , Células A549/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Diterpenos Clerodânicos/isolamento & purificação , Relação Dose-Resposta a Droga , Humanos , Concentração Inibidora 50 , Células MCF-7/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Extratos Vegetais/farmacologia
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