RESUMO
Soil microplastic pollution is ubiquitous, but the vertical distribution characteristics of microplastics in different land use types are unclear. In this study, the microplastic abundance, particle size, shape, color, and polymer type in 0-20 cm, 20-40 cm, and 40-60 cm soil layers of seven land use types (woodland, grassland, maize, wheat, cotton, polytunnel, and greenhouse) were systematically investigated in Shouguang City, a typical agricultural city in China. The results showed that the average microplastic abundance from top to deep for the three soil layers of Shouguang City were 1948.1 ± 992.5, 1349.4 ± 654, and 670.1 ± 341.6 items kg-1. In the top soil layer (0-20 cm), the abundance of microplastics in facility soils was significantly higher than in other land use types. In agricultural soils, microplastics were predominantly small size (<0.5 mm), films and fragments, PE and PP. The average microplastic abundance in deep soils (40-60 cm) of the seven land use types was 349.1 ± 62.8 (woodland), 284.9 ± 113.9 (grassland), 657.1 ± 127.1 (maize), 537.8 ± 137.4 (wheat), 851.4 ± 204.2 (cotton), 878.5 ± 295.7 (polytunnel), 1132.2 ± 304.5 (greenhouse) items kg-1, respectively, accounting for 11 % to 19 % in all three soil layers. The percentage of small size and pellet microplastics increased in deep soils (40-60 cm). Correlation analysis showed that soil environmental factors (pH, EC, total phosphorus, total nitrogen, and organic carbon) influenced to different extents the distribution, fragmentation, and transport of microplastics. The results of this study contribute to a better understanding of contamination and vertical distribution of soil microplastics in agricultural and non-agricultural soils, as well as provide important data for the development of preventive and management policies.
RESUMO
A potent antitumor CD4(+) T-helper cell immune response is created by inducing tumor cells in vivo to a MHC class II(+)/Ii(- )phenotype. MHC class II and Ii molecules were induced in tumor cells in situ following tumor injection of a plasmid containing the gene for the MHC class II transactivator (CIITA). Ii protein was suppressed by the antisense effect of an Ii-reverse gene construct (Ii-RGC) in the same or another co-injected plasmid. The MHC class II(+)/Ii(- )phenotype of the tumor cells was confirmed by FACS analysis of cells transfected in vitro and by immunostaining of tumor nodules transfected by injections in vivo. Subcutaneous Renca tumors in BALB/c mice were treated by intratumoral injection with CIITA and Ii-RGC, in combination with a subtherapeutic dose of IL-2, to up-regulate the activation of T cells. Significant tumor shrinkage and decrease in rates of progression of established Renca tumors were seen in the groups injected with Ii-RGC, compared with groups in which only IL-2 plus empty plasmid controls were injected. Our method provides an effective immunotherapy warranting further development for human cancers.