Smart cost estimation: Empirical case for extra-high voltage transmission towers.

Nowadays, electricity has become an integral part of human lives. Most of our daily appliances, tools, and personal belongings are inseparable from electricity. To ensure a proper electricity distribution with an efficient transfer capability, Extra-High Voltage (EHV) transmission towers are needed. To design such a structure, it is of utmost importance to account for the cost of said tower. However, the process to estimate the cost of EHV transmission towers is both time-consuming and strenuous on human labor since a lot of consideration have to be taken. To overcome this, an imperative requirement exists for a prompt, precise, and automated tool to replace the existing manual cost estimation method. This research endeavor aims to craft a tool using support vector regression (SVR) with the capacity to prognosticate construction expenses for projects involving EHV transmission towers. The exploration of pertinent literature has enabled us to amass historical data and delineate the attributes essential for estimating costs linked to EHV transmission tower construction. The investigation delves into a comprehensive dataset spanning the past decade in Taiwan. Within this timeframe, 317 EHV transmission towers were erected between 2009 and 2019. However, 79 of these instances are excluded due to incomplete information, thereby yielding 238 viable datasets (comprising 75 % of the overall total) to underpin the development of the SVR model. By configuring the parameters to C = 0.2 and γ = 0.1, followed by 5-fold cross-validation, the resultant SVR model attains a remarkable prediction accuracy of 97.91 %, on average. As a result, the proposed SVR-based model can effectively and accurately predict the cost of constructing an EHV transmission tower project and reduce the time spent on estimation, thus contributing to the enhancement of the resilience and robustness of the transmission network system.

ADCK1 is a potential therapeutic target of osteosarcoma.

We here showed that ADCK1 (AarF domain-containing kinase 1), a mitochondrial protein, is upregulated in human osteosarcoma (OS) tissues and OS cells. In primary and established OS cells, ADCK1 shRNA or CRISPR/Cas9-induced ADCK1 knockout (KO) remarkably inhibited cell viability, proliferation and migration, and provoked apoptosis activation. Conversely, ectopic ADCK1 overexpression exerted pro-cancerous activity by promoting OS cell proliferation and migration. ADCK1 depletion disrupted mitochondrial functions in OS cells and induced mitochondrial membrane potential reduction, ATP depletion, reactive oxygen species production. Significantly, ADCK1 silencing augmented doxorubicin-induced apoptosis in primary OS cells. mTOR activation is important for ADCK1 expression in OS cells. The mTOR inhibitors, rapamycin and AZD2014, as well as mTOR shRNA, potently decreased ADCK1 expression in primary OS cells. In nude mice, the growth of subcutaneous pOS-1 xenografts was largely inhibited when bearing ADCK1 shRNA or ADCK1 KO construct. Moreover, ADCK1 KO largely inhibited pOS-1 xenograft in situ growth in proximal tibia of nude mice. ADCK1 depletion, apoptosis activation and ATP reduction were detected in pOS-1 xenografts bearing ADCK1 shRNA or ADCK1 KO construct. Together, the mitochondrial protein ADCK1 is required for OS cell growth and is a novel therapeutic target of OS.

Exploring Public Discussions Regarding COVID-19 Vaccinations on Microblogs in China: Findings from Machine Learning Algorithms.

Large-scale, widespread COVID-19 vaccination is the most effective means of cutting off the spread of the novel coronavirus and establishing an immune barrier. Due to the large population base in China, it has been a very difficult task to establish such an immune barrier. Therefore, this study aims to explore the public's discussions related to COVID-19 vaccinations on microblogs and to detect their sentiments toward COVID-19 vaccination so as to improve the vaccination rate in China. This study employed machine learning methods in the field of artificial intelligence to analyze mass data obtained from SinaWeibo. A total of 1,478,875 valid microblog texts were collected between December 2020 and June 2022, the results of which indicated that: (1) overall, negative texts (38.7%) slightly outweighed positive texts (36.1%); "Good" (63%) dominated positive texts, while "disgust" (44.6%) and "fear" (35.8%) dominated negative texts; (2) six overarching themes related to COVID-19 vaccination were identified: public trust in the Chinese government, changes in daily work and study, vaccine economy, international COVID-19 vaccination, the COVID-19 vaccine's R&D, and COVID-19 vaccination for special groups. These themes and sentiments can clarify the public's reactions to COVID-19 vaccination and help Chinese officials' response to vaccine hesitancy. Furthermore, this study seeks to make up for the lack of focus on big data in public health and epidemiology research, and to provide novel insights for future studies.

Molecular identification of Colpodella sp. of South China tiger Panthera tigris amoyensis (Hilzheimer) in the Meihua Mountains, Fujian, China.

A three-year-old male South China tiger died in the tiger enclosure of the China Tiger Park in the Meihua Mountains on December 2018 after being bitten by a tick. This tiger presented clinical symptoms like whole-body severe jaundice, hepatosplenomegaly, kidney, and lymph node hemorrhages. The Colpodella sp.-specific 18S rRNA gene was detected using nested PCR. Interestingly, the DNA isolated from the blood of the tiger was found to be 100% similar to that of the tick by NCBI BLAST analysis. However, the DNA fragments isolated from the tiger's blood were 90.1% similar to the Colpodella sp. strain human erythrocyte parasite (HEP, MH208621) and 90.4% similar to the Colpodella sp. strain Heilongjiang (HLJ, KT364261). To investigate the species of ticks and ticks-carried Colpodella parasites in this region, the species of ticks obtained from the grasses outside the tiger enclosure and the species of Colpodella carried by ticks were identified. The DNA from ticks as well as that from the tick-borne Colpodella sp. were amplified from each tick using PCR followed by amplicon sequencing. In total 402 adult ticks samples were collected, among which 22 were positive for Colpodella sp. (5.5%), and the species were further determined by morphology, DNA sequencing and phylogenetic analyses. Interestingly, one Colpodella sp. was found to have 94.2% sequence similarities to the Colpodella sp. strain HEP (MH208621). This strain was previously reported to infect a woman in Yunnan, China. In addition, three Colpodella sp. showed 87-91% sequence similarities to the Colpodella sp. strain HLJ (KT364261), which was previously reported to infect human in Heilongjiang, China. This study disclosed the possibility of zoonotic transmission of Colpodella sp. by ticks in China. Finally, it provides a basis for urgently determining and monitoring the repertoire of ticks-borne piroplasmid pathogens, with the ultimate aim of strategic control.

A study on evaluation of laparoscopic surgical approach for pediatric appendix abscess.

BACKGROUND: The main objective of this study was to investigate and compare chosen time laparoscopic surgery to traditional open surgery and to analyze whether laparoscopic surgery is safe and feasible for pediatric appendix abscess in emergency. METHODS: This retrospective study consisted of three groups of patients designated as group A, group B, group C and the preoperative and postoperative clinical characteristics were analyzed and compared. Group A comprised of 63 patients of appendix abscess which has been treated by laparoscopic surgery from January 2011 to December 2014. Group B comprised of 60 patients who had undergone pediatric appendix abscess laparotomy and group C comprised of 35 cases who had undergone time-selective laparoscopic appendix ablation surgery after receiving anti-inflammatory treatment. RESULTS: The average operation time during which all the appendix ablated successfully between group A and group B patients was not significantly different (P>0.05), meanwhile, operation time was significantly less for group C patients in comparison with group A patients (P<0.05). The incidence of postoperative complications among patients of group A was significantly lower than that of group B (P<0.05), while, these complications were similarly distributed between group A and group C (P>0.05). The duration of hospitalization among patients of group B (P<0.05) and group C (P<0.05) was significantly higher in comparison with group A patients. CONCLUSIONS: As long as preoperative and perioperative periods are appropriately dealt with, laparoscopic surgery is safe and feasible to pediatric appendix abscess in emergency.

Interleukin-24 inhibits the phenotype and tumorigenicity of cancer stem cell in osteosarcoma via downregulation Notch and Wnt/ß-catenin signaling.

Osteosarcoma frequently presents as recurrence and metastasis, even if the primary lesion was eradicated and/or radiotherapy and chemotherapy were administered. Osteosarcoma cancer stem cells (CSCs) are one of the key factors for the recurrence and metastasis of osteosarcoma. We have shown that interleukin-24 (IL-24) inhibits osteosarcoma cell proliferation, migration and invasion in vitro. In the current study, we investigated the role of IL-24 in inhibiting the growth of osteosarcoma CSCs. IL-24 inhibited proliferation and invasion and decreased the stemness of osteosarcoma CSCs in vitro. In a nude mouse xenograft model, IL-24 significantly inhibited the growth of tumors originating from osteosarcoma CSCs. Moreover, we found that IL-24 was able to inactivate both Notch and Wnt/ß-Catenin signaling, which are important for the development of the biological characteristics of CSCs. These data demonstrate that IL-24 is able to kill not only cancer cells but also CSCs in osteosarcoma, suggesting that IL-24 might eradicate osteosarcoma and enhance long-term cure rates in patients with osteosarcoma.

Quantitative assessment of the effects of biochar amendment on photosynthetic carbon assimilation and dynamics in a rice-soil system.

Biochar amendment has been proposed as a promising means to increase carbon (C) sequestration and simultaneously benefit plant productivity. However, quantifying the assimilation and dynamics of photosynthetic C in plant-soil systems under biochar addition remains elusive. This study established two experimental factors involving biochar addition and nitrogen (N) fertilization to quantitatively assess the effect of biochar on photosynthetic C fate in a rice plant-soil system. The rice plants and soil samples were collected and analyzed after 6-h pulse labeling with 13 CO2 at the tillering, jointing, heading and ripening stages. Biochar did not affect the proportions of photoassimilated carbon-13 (13 C) allocations in plant-soil systems. Nevertheless, biochar enhanced the 13 C contents in the shoot, root, and soil pools, especially when combined with N fertilization, and biochar increased the cumulative assimilated 13 C contents in the shoot, root, and soil pools by 23%, 14% and 20%, respectively, throughout the whole growth stage. Moreover, biochar addition significantly enhanced the N use efficiency (NUE) by c. 23% at the heading and ripening stages. In summary, biochar increases the content of photoassimilated C in plant-soil systems by improving plant productivity via enhancing NUE, thus resulting in a higher soil C sequestration potential.

Expressions of Fib, IL-12 in Serum of Neonatal Necrotizing Enterocolitis and Their Correlation with Clinicopathological Features.

BACKGROUND: To investigate the expressions of fibrinogen (Fib) and Interleukin-12 (IL-12) in serum of neonatal necrotizing enterocolitis (NEC), and to analyze the correlation between the two and their relationship with clinicopathological features. METHODS: Forty two children with NEC treated in Xuzhou Children's Hospital, Xuzhou Medical University Xuzhou, China from 2016-2019 were selected as an observation group and 40 children who underwent physical examination at the same period as a control group. The expression levels of Fib and IL-12 in the serum of two groups were detected by ELISA. The correlation between Fib and IL-12 in the observation group and the correlation among the expressions of Fib, IL-12, the clinicopathological features and common examination indexes of the children with NEC were investigated by Pearson correlation analysis. RESULTS: The levels of Fib and IL-12 in the serum of the children in observation group were significantly higher than those in the control group were (P<0.05). There was a significant positive correlation between the levels of Fib and IL-12 in the serum of the children in observation group (P<0.05). The expression levels of Fib, IL-12 were not significantly correlated with sex and age of NEC children, but correlated with vomiting, diarrhea, bloody stool and bradycardia in NEC children (P<0.05). Fib and IL-12 were positively correlated with erythrocyte level (P<0.05) and negatively correlated with platelet level. CONCLUSION: The expressions of Fib and IL-12 in the serum of NEC children can objectively predict the severity of NEC.

Curcumin improves necrotising microscopic colitis and cell pyroptosis by activating SIRT1/NRF2 and inhibiting the TLR4 signalling pathway in newborn rats.

This study aimed to explore comprehensively the biological function of curcumin, and its underlying mechanism, in protecting from necrotising microscopic colitis in newborn rats. A total of 20 normal healthy rats were selected, and a necrotising enterocolitis (NEC) model was established. After hypoxia and hypothermia stimulation, these rats were treated with different doses of curcumin (control group, NEC model group, NEC+20 mg/kg curcumin and NEC+50 mg/kg curcumin). Inflammation was identified using hematoxylin and eosin staining, and inflammatory factors were detected via ELISA. The mRNA and protein levels of SIRT1, NRF2, TLR4, NLRP3 and caspase-1 were determined by quantitative RT-PCR and Western blotting, respectively. Curcumin improved the inflammatory condition of NEC and inhibited the expression of inflammatory factors in NEC newborn rat intestinal tissue. Furthermore, the SIRT1/NRF2 pathway was inhibited in the intestinal tissue of NEC newborn rats, whereas curcumin treatment induced the activation of the SIRT1/NRF2 pathway and inhibited TLR4 expression in these animals. In addition, curcumin could also inhibit the expression of inflammatory factors and alleviate the LPS/ATP-induced focal death pathway in intestinal epithelial cells through the SIRT1 pathway. Curcumin can improve necrotising microscopic colitis and cell pyroptosis by attenuating NEC-induced inhibition of SIRT1/NRF2 and inhibiting the TLR4 signalling pathway in newborn rats.

Flexure Behaviors of ABS-Based Composites Containing Carbon and Kevlar Fibers by Material Extrusion 3D Printing.

Short-fiber-reinforced thermoplastics are popular for improving the mechanical properties exhibited by pristine thermoplastic materials. Due to the inherent conflict between strength and ductility, there are only a few successful cases of simultaneous enhancement of these two properties in polymer composite components. The objective of this work was to explore the feasibility of simultaneous enhancement of strength and ductility in ABS-based composites with short-carbon and Kevlar fiber reinforcement by material extrusion 3D printing (ME3DP). Microstructure characterization and measurement of thermal and mechanical properties were conducted to evaluate the fiber-reinforced ABS. The influence of printing raster orientation and build direction on the mechanical properties of material extrusion of 3D-printed composites was analyzed. Experimental results demonstrated that the reinforcement of the ABS-based composites by short-carbon and Kevlar fibers under optimized 3D-printing conditions led to balanced flexural strength and ductility. The ABS-based composites with a raster orientation of ±45° and side build direction presented the highest flexural behaviors among the samples in the current study. The main reason was attributed to the printed contour layers and the irregular zigzag paths, which could delay the initiation and propagation of microcracks.

Anti-cancer effect of oncolytic adenovirus-armed shRNA targeting MYCN gene on doxorubicin-resistant neuroblastoma cells.

Chemotherapy is one of the few effective choices for patients with neuroblastoma. However, the development of muti-drug resistance (MDR) to chemotherapy is a major obstacle to the effective treatment of advanced or recurrent neuroblastoma. The muti-drug resistance-associated protein (MRP), which encodes a transmembrane glycoprotein, is a key regulator of MDR. The expression of MRP is a close correlation with MYCN oncogene in neuroblastoma. We have recently shown ZD55-shMYCN (oncolytic virus armed with shRNA against MYCN) can down-regulate MYCN to inhibit tumor cells proliferation and induce apoptosis in neuroblastoma. Here we further report ZD55-shMYCN re-sensitized doxorubicin-resistant cells to doxorubicin (as shown by reduced proliferation, increased apoptosis, and inhibited cell migration), and reduced the in vivo growth rate of neuroblastoma xenografts by down-regulation of MRP expression. Sequential therapy with doxorubicin did not affect the replication of ZD55-shMYCN in doxorubicin-resistant neuroblastoma cells, but decreased the expression of Bcl-2, Bcl-XL, MMP-1. Thus, this synergistic effect of ZD55-shMYCN in combination with doxorubicin provides a novel therapy strategy for doxorubicin-resistant neuroblastoma, and is a promising approach for further clinical development.

Metabolic Adaptations in Pregnancy: A Review.

BACKGROUND: Pregnancy is a dynamic state involving multiple adaptations that are necessary in order to ensure a continuous supply of essential metabolites to support the growth and the development of the fetus. OBJECTIVES: This review article is aimed to discuss important adaptations in metabolism that take place during non-complicated pregnancy. MATERIALS AND METHODS: We searched the electronic database PubMed for pre-clinical as well as clinical controlled trials reporting the importance of metabolic adaptations during a non-complicated pregnancy. The preferred language was English and the most recent reports were selected to get an updated review. RESULTS: It was observed clearly in the searched literature that metabolic adaptations are a crucial part of pregnancy, as they provide the mother with sufficient energy stores to meet the demands of pregnancy. These adaptions also help in preparing the mother for lactation and also help in providing proper environment for the proper growth of fetus in the womb. Moreover, multiple biomolecules including glucose, fatty acids, ketone bodies, hormones collectively contribute toward these metabolic adaptations. CONCLUSIONS: This review article concludes that metabolic adaptations are crucial for proper fetus development.

Retrospective Clinical Study of Maxillary Sagittal Fractures: Predictors of Postoperative Outcome.

PURPOSE: Because of less attention to the sagittal component of maxillary fractures, these fractures are often misdiagnosed or the reduction is missed leading to maxillary transverse discrepancies. Therefore, the purpose of this study was to identify factors associated with good or adverse postoperative outcomes of maxillary sagittal fractures. MATERIALS AND METHODS: This study was a single-center retrospective cohort study. The sample was composed of cases of maxillary sagittal fractures treated at the Department of Oral and Maxillofacial Surgery, Craniomaxillofacial Trauma Unit of Xi'an Jiaotong University (Xi'an, China) from January 2008 through December 2013. The predictor variables were age, gender, occupation, cause of injury, injury severity, treatment timing, treatment method, and quality of fracture reduction. The outcome variable was the postoperative treatment effect index. Descriptive, bivariate, and multivariate statistics were computed. The P value was set to .05. RESULTS: The sample was composed of 40 cases. The male-to-female ratio was 4:1; the most vulnerable age group was 20 to 30 years (30%); laborers (72.5%) were more prone to injury; and the main cause of injury was motor vehicle accident (62.5%). No cases of isolated sagittal fracture were found and most (35%) occurred with other maxillary fractures, including Le Fort fractures. A statistically significant association between treatment timing and quality of fracture reduction and the postoperative treatment effect index (P < .05) was found. CONCLUSION: The results of this study suggest that better results are achieved when fractured bone is treated sooner. Anatomic repositioning of the fractured bone is the important predictor for good postoperative outcomes.

Pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway.

Pingyangmycin (also known as Bleomycin A5) is produced by Streptomyces verticillus var. pingyangensis n.sp., and has anti­tumor activities against a variety of tumor cells. The aim of the present study was to determine the molecular mechanism(s) underlying the therapeutic effects of pingyangmycin against infantile hemangiomas. Human hemangioma­derived endothelial cells (HemECs) were treated with pingyangmycin at varying concentrations (100, 200 or 300 µg/ml), and the morphological changes and apoptosis levels were assessed. The gene expression changes were determined by cDNA microarray technology. Transmission electron microscopy examination revealed that the pingyangmycin­treated HemECs exhibited typical apoptotic characteristics, including chromatin condensation and the formation of apoptotic bodies. Annexin­V staining demonstrated that pingyangmycin caused a significant and dose­dependent induction of apoptosis in the HemECs. In the pingyangmycin­treated HemECs, 4,752 genes demonstrated at least 2­fold expression changes at the mRNA level. Quantitative polymerase chain reaction confirmed that pingyangmycin significantly upregulated the expression of p53, p53­induced protein with death domain, Bax, p53 upregulated modulator of apoptosis and p53 inducible gene 3, and downregulated the expression of murine double minute 2. The data demonstrated that the pro­apoptotic activity of pingyangmycin against infantile hemangiomas involves p53 pathway activation.

Facile synthesis and versatile topological transformation of mono-cleavable symmetric starlike terpolymers.

A straightforward strategy involving a RAFT process and ring-opening polymerization was used to construct symmetric reduction-responsive amphiphilic A(2m)B(2n)C(2) (m≈n≈ 3) starlike terpolymers with precise microstructure, which could be efficiently converted into thiol-functionalized telechelic stars, degraded A(m)B(n)C miktoarm stars and comblike-linear multiblock copolymers via postmodification.