RESUMO
OBJECTIVES: To study the effect of γ irradiation on ß-adrenergic receptors of the lung. MATERIALS AND METHODS: Healthy Sprague-Dawley rats were used as an animal model. Cell membrane proteins of lung tissue were harvested after the whole lung received 20 Gy of 60Co γ irradiation. 125I-labeled iodopindolol (125I-IPIN) was used as a ligand of ß-adrenergic receptors. The numbers of the ß-adrenergic receptors were determined by radioligand-receptor binding assay (RBA). Data were compared with irreversible blockage using antagonist bromoacetylalprenololmenthan (BAAM). RESULTS: The post-radiation RBA assay showed that the number of ß-adrenergic receptors in lung tissue decreased at a steady rate. It decreased to 48% of the normal level at the 15th day after irradiation. At 40 days after radiation the level of ß-adrenergic receptors started to increase at a steady rate and reached to the normal level around 70 days after radiation. There were significant differences in receptor synthesis, degradation and regeneration rates between irradiation group and BAMM group. CONCLUSIONS: The whole lung irradiation could severely affect the levels of ß-adrenergic receptors. The potential clinical implications of radiation-induced changes of ß-adrenergic receptors warrant further investigation.
Assuntos
Raios gama , Pulmão/efeitos da radiação , Receptores Adrenérgicos beta/metabolismo , Receptores Adrenérgicos beta/efeitos da radiação , Antagonistas Adrenérgicos beta/farmacologia , Alprenolol/análogos & derivados , Alprenolol/farmacologia , Animais , Feminino , Cinética , Pulmão/química , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley , Receptores Adrenérgicos beta/efeitos dos fármacosRESUMO
The aim of this in vivo study was to evaluate the feasibility of
Assuntos
Processamento de Imagem Assistida por Computador/métodos , Medições Luminescentes/métodos , Imagem Óptica/métodos , Compostos de Organotecnécio/química , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Animais , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/farmacocinética , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias Experimentais/química , Neoplasias Experimentais/diagnóstico por imagem , Neoplasias Experimentais/metabolismo , Compostos de Organotecnécio/farmacocinética , Proteoglicanas/química , Proteoglicanas/farmacocinética , Reprodutibilidade dos Testes , Imagem Corporal TotalRESUMO
OBJECTIVE: To investigate the feasibility of using cartilage link protein of hyaluronic acid (HA-CLP) for defining the tumor boundary in a mouse model of lung carcinoma. METHODS: Lung carcinoma was induced in KM mice by chemical carcinogenesis. HA-CLP separated from bovine cartilage and purified by affinity chromatography was labeled with (125)I for autoradiography. Immunohistochemical analysis and Western blotting were used to examine the efficiency of HA-CLP in defining the boundaries of the lung tumors. RESULTS: With autoradiography, the clearest image of lung cancer was obtained at 2 h. With immunohistochemical method, the tumor boundary was the most clearly displayed at 2 h when the strongest signals of HA-CLP was detected; Western blotting also showed the clearest bands of HA-CLP at 2 h. CONCLUSION: HA-CLP has the immunogenicity of HABP, and can efficiently indicate lung tumor boundary in autoradiography and immunohistochemistry.