In vitro rumen degradability of tropical legumes and their secondary metabolites depends on inoculum source.

In this study, the in vitro apparent rumen degradability of organic matter (ARDOM) and plant secondary metabolites (ARDPSM) of three tropical legumes (Mucuna pruriens, Canavalia ensiformis, and Leucaena leucocephala) were assessed. For this, 3 experiments were set up, i.e., single end-point incubations (24 h) with ruminal inoculum from either Belgian or Cuban sheep, as well as kinetic assessments (0 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, and 24 h) inoculum from Belgian sheep. L-mimosine, L-canavanine, Concanavalin A (Con A), and trypsin inhibitor (TI) were the plant secondary metabolites (PSM) targeted in this study. In all three experiments, both beans, as well as forage/bean meals of M. pruriens and C. ensiformis and their PSM, were extensively degraded during 24 h incubation, irrespective of the inoculum source (0.44 to 0.70 and 0.43 to 0.78 g/g of organic matter (OM) for ARDOM, respectively, and > 0.80 g/g for L-canavanine, > 0.76 TIU/TIU for TI, and > 0.95 g/g for Con A, for both legumes). Forage meal of L. leucocephala was considerably less degraded, with apparent ruminal degradabilities of 0.20 g/g OM and 0.35 g/g OM after 24 h incubation with Belgian or Cuban sheep inoculum, respectively. This could - at least partially - be related to L-mimosine, present in L. leucocephala, which was hardly degraded in the Belgian incubation, while a more extensive ruminal breakdown was observed under the Cuban conditions (0.05 g/g PSM vs. 0.78 g/g PSM, respectively). The negative effect of L-mimosine on OM degradability was supported in an additional in vitro experiment with straw and inoculum from Belgian sheep, as ruminal degradation of straw was 31% lower when pure L-mimosine was supplemented.

Influence of Acacia Mearnsii Fodder on Rumen Digestion and Mitigation of Greenhouse Gas Production.

In recent years, the worrying generation of GHG from ruminant production has generated widespread interest in exploring nutritional strategies focused on reducing these gases, presenting the use of bioactive compounds (tannins) as an alternative in the diet. The aim of this research was to determine the effect of the addition of different levels of Acacia mearnsii on ruminal degradation, nutrient digestibility, and mitigation of greenhouse gas production. A completely randomized design with four treatments and six repetitions was used. The treatments were: T1, T2, T3, and T4 diets with, respectively, 0%, 20%, 40%, and 60% A. mearnsii. The rumen degradation kinetic and in vitro digestibility, and the production of gas, CH4, and CO2 were evaluated. In situ rumen degradation and in vitro digestibility of DM and OM showed differences between treatments, with T1 being higher (p < 0.05) in the degradation of the soluble fraction (A), potential degradation (A + B), and effective degradation for the different passage rates in percent hour (0.02, 0.05, and 0.08), compared to the other treatments. Rumen pH did not show differences (p > 0.05) between treatments. The lowest (p < 0.05) gas, CH4, and CO2 production was observed in treatments T1 and T2 with an approximate mean of 354.5 mL gas/0.500 g fermented DM, 36.5 mL CH4/0.500 g fermented DM, and 151.5 mL CO2/0.500 g fermented DM, respectively, compared to treatments T3 and T4. Under the conditions of this study, it was concluded that it is possible to replace traditional forages with up to 20% of A. mearnsii, without observing changes in the production of greenhouse gases with respect to the control treatment (0% of A. mearnsii); however, A. mearnsii is not usable because it significantly decreases rumen degradability of DM and OM, which would considerably affect the production in animals.

Parenteral supplementation of Cu, Zn and Mn enhances boars' sperm quality.

INTRODUCTION: The trace elements concentrations of blood might play a key role in the trace element concentration of seminal plasma, as well as in the improvement of semen volume and sperm morphology in boars. OBJECTIVE: To assess the effect of supplementation of Cu, Zn and Mn on their concentrations in blood serum and seminal plasma and sperm quality in boars. METHODS: Eighteen boars of the Duroc Jersey breed were selected and their blood and semen (54 ejaculates before supplementation) were extracted. Coinciding with the third sampling and after of blood taken, the boars were supplemented subcutaneously with 37.5 mg of Cu, 75 mg of Zn and 37.5 mg of Mn; 40 days after the supplementation the fourth sampling was taken. Cu and Zn concentrations in blood serum and seminal plasma were determined by atomic absorption spectrophotometry, while the sperm pathologies were determined by microscopy and the concentrations of Cu and Zn in blood serum and seminal plasma, and semen pathologies were compared using the t-Student test for paired samples. A simple linear correlation was made between the minerals concentration in seminal plasma with the percentage of spermatozoa with abnormal forms. RESULTS: Although the concentrations of Zn in blood serum did not show differences between sampling periods (P < 0.05), they were significantly higher (P < 0.01) in the seminal plasma after supplementation as compared to its levels before supplementation. In addition, the concentrations of Cu and Zn in seminal plasma were increased (P < 0.01), and the total spermatic pathologies were reduced; especially those of the head, neck and intermediate part of the tail (P < 0.001). Besides, macrocephaly, double head and broken acrosome were the most common pathologies (P < 0.05). Moreover, parenteral supplementation of Cu, Zn and Mn was a protective factor to the presentation of ejaculates with abnormal sperm percentages higher than 10% (χ2 = 6.1544; P = 0.0131). The prevalence of abnormal shapes of boars' sperm before supplementation was 0.40; after supplementation the answer was 0.05 and the prevalence ratio was 0.13 with a confidence interval of 95% from 0.01 to 0.94. Moreover, Zn concentrations in blood serum were not correlated with those of the seminal plasma (P > 0.05, r = - 0.0353); however, the concentrations of Cu in both fluids were correlated (P < 0.05, r = 0.2254). In addition, the Zn values in the seminal plasma and the percentage of abnormal spermatozoa had a negative and highly significant correlation (P < 0.0001, r = - 0.5628). However, the Cu concentrations in the semen were not significantly correlated with the abnormal sperm forms (P > 0.05, r = 0.0200). CONCLUSION: From the present study it can be concluded that in boars fed with diets that meeting their requirements in trace minerals according to NRC (2012) [1], parenteral supplementation of 37.5 mg of Cu, 75 mg of Zn and 37.5 mg of Mn increased the Zn concentrations in the seminal plasma and reduced the sperm pathologies, which resulted in an increase of the boars' sperm quality.

Performance, Rumen Microbial Community and Immune Status of Goat Kids Fed Leucaena leucocephala Post-weaning as Affected by Prenatal and Early Life Nutritional Interventions.

Leucaena leucocephala represents a local protein source in tropical ruminant diets. However, its full exploitation is impaired by mimosine, unless it is degraded by the rumen microbial community. Recently, the ruminal bacterial communities of newborns were persistently modified through prenatal or postnatal dietary interventions. Such early-life interventions might enhance adaptation of ruminants to Leucaena leucocephala, which was investigated using a 2 × 2 factorial design trial that tested both supplementation of L. leucocephala in the late pregnancy diet of goat does, and supplementation of live yeast to their newborns. The composition of ruminal bacteria, immune status, as well as organic matter digestibility (OMD) and performance of kids were studied during and after the intervention. Ten pregnant goats were divided into two groups: the D+ and D- groups, which either received or did not receive 30 g of L. leucocephala forage meal during the last 7 ± 0.5 weeks of gestation. Twins from each goat were divided into the K+ and K- group (supplemented with or without 0.2 g/d of live yeast from day 3 until weaning at 8 weeks). Rumen samples were collected from 4-, 8-, 14-, and 20-weeks old kids to assess the bacterial community, while immune parameters (white blood cells, immunoglobulin M and G, and chitotriosidase activity) were measured in blood and saliva sampled at 4-, 8-, and 20-weeks. We found a stimulatory effect of the prenatal exposure on the post-weaning dry matter intake of the L. leucocephala supplemented diet, resulting in a higher daily gain and final body weight at 20 weeks in the D+ vs. D- group (406 vs. 370 g DM/d, 85.4 vs. 78.6 g/d, and 15.2 vs. 13.8 kg, respectively). Moreover, Ruminococcus represented a greater proportion of the rumen bacterial community of the D+ vs. D- kids (5.1 vs. 1.6%). Differences in the immune status were relatively small and not thought to be a driving factor of differences in animal performance. Furthermore, postnatal supplementation of live yeast favored maturation of the rumen bacterial community (i.e., greater abundance of Bacteroidetes, in particular Prevotella, and reduced abundance of Firmicutes) and protozoa colonization. Concomitantly, OMD was enhanced post-weaning, suggesting effects of the early-life intervention persisted and could have affected animal performance.