Recent reports on the sensing strategy and the On-site detection of illegal drugs.

In this review, works on the on-site detection of illegal drugs in recent years are summarised and discussed, most of which were published within the past five years. The detection methods are categorised as colourimetric, fluorescence, Raman spectrometry, ion mobility spectrometry, electrochemistry, and mass spectrometry. Also, strategies that are possibly suitable for on-site detection and the actual instrumentation to be used in the field are listed.

Humidity Enhances the Solid-Phase Catalytic Ability of a Bulk MOF-808 Metal-Organic Gel toward a Chemical Warfare Agent Simulant.

Zirconium-based metal-organic frameworks have emerged as promising materials for detoxifying chemical warfare agents (CWAs) due to their remarkable stability and porosity. However, their practical application is hindered by issues with their powder form and poor catalytic performance in solid-phase degradation. To address these challenges, herein, a granular MOF-808 metal-organic gel (G808) is prepared under optimized conditions for catalytic degradation of the simulant 2-chloroethyl ethyl sulfide (2-CEES), a sulfide blister agent, in a neat state under different humidity conditions. The detoxification performance of G808 toward 2-CEES is significantly enhanced as the content of water present increases. The half-life of 2-CEES decontaminated by G808 can be shortened to 816 s, surpassing those of many other benchmark materials. To confirm the mechanism of catalytic degradation, we used gas chromatography, gas chromatography-mass spectrometry, and theoretical calculations. The findings revealed that hydrolysis was the predominant route. Additionally, granular G808 was reusable and adaptable to high-moisture environments, making it an excellent protective material with practical potential.

Using Wool Keratin as a Structural Biomaterial and Natural Mediator to Fabricate Biocompatible and Robust Bioelectronic Platforms.

The design and fabrication of biopolymer-incorporated flexible electronics have attracted immense interest in healthcare systems, degradable implants, and electronic skin. However, the application of these soft bioelectronic devices is often hampered by their intrinsic drawbacks, such as poor stability, inferior scalability, and unsatisfactory durability. Herein, for the first time, using wool keratin (WK) as a structural biomaterial and natural mediator to fabricate soft bioelectronics is presented. Both theoretical and experimental studies reveal that the unique features of WK can endow carbon nanotubes (CNTs) with excellent water dispersibility, stability, and biocompatibility. Therefore, well-dispersed and electroconductive bio-inks can be prepared via a straightforward mixing process of WK and CNTs. The as-obtained WK/CNTs inks can be directly exploited to design versatile and high-performance bioelectronics, such as flexible circuits and electrocardiogram electrodes. More impressively, WK can also be a natural mediator to connect CNTs and polyacrylamide chains to fabricate a strain sensor with enhanced mechanical and electrical properties. With conformable and soft architectures, these WK-derived sensing units can be further assembled into an integrated glove for real-time gesture recognition and dexterous robot manipulations, suggesting the great potential of the WK/CNT composites for wearable artificial intelligence.

Using Wool Keratin Derived Metallo-Nanozymes as a Robust Antioxidant Catalyst to Scavenge Reactive Oxygen Species Generated by Smoking.

Self-assembled nanostructures based on biomolecules (e.g., proteins and amino acids) and metal ions have promising applications in mimicking the nanostructure, properties, and functions of natural enzymes. Herein, a metal ion-mediated self-assembly method for constructing catalytically active Cu-wool-keratin (CuWK) two-dimensional nanozymes is presented. Specifically, by introducing copper ions as abiological cofactors, WK can serve as a protein scaffold to design and create Cu catalytic sites. The optimized hybrids with Cu-WK coordination framework exhibit significant superoxide dismutases-like activity, catalase-like activity, and hydroxyl radical scavenging ability. These combined antioxidant activities make CuWK a robust nanozyme to effectively remove various reactive oxygen species (ROS). In this work, the as-prepared CuWK as a new additive can be integrated into a cigarette filter system to effectively remove the produced ROS from the burning of tobacco. More importantly, the CuWK nanozymes as a critical element can be further utilized to construct a recycling cigarette holder. Therefore, the present work shows that nanozymes with advanced catalytic capabilities can be constructed by self-assembly of metal ions and proteins, thus facilitating the rational design and discovery of this kind of artificial metalloenzymes.

Recent Advances in Patterning Natural Polymers: From Nanofabrication Techniques to Applications.

The development of a flexible and efficient strategy to precisely fabricate polymer patterns is increasingly significant for many research areas, especially for cell biology, pharmaceutical science, tissue engineering, soft photonics, and bioelectronics. Recent advances of patterning natural polymers using various nanofabrication techniques, including photolithography, electron-beam lithography, dip-pen nanolithography, inkjet printing, soft lithography, and nanoimprint lithography are discussed here. Integrating nanofabrication techniques with naturally derived macromolecules provides a feasible route for transforming these polymer materials into versatile and sophisticated devices while maintaining their intrinsic and excellent properties. Furthermore, the corresponding applications of these natural polymer patterns generated by the above techniques are elaborated. In the end, a summary of this promising research field is offered and an outlook for the future is given. It is expected that advances in precise spatial patterns of natural polymers would provide new avenues for various applications, such as tissue engineering, flexible electronics, biomedical diagnosis, and soft photonics.

Automatic Sensing Setup for Methamphetamine Based on the Reactional Wettability Variation Strategy.

An automatic setup for reactional wettability variation (RWV) was developed by interlinking liquid selection and transportation, object movement, and image recognition. In this way, the performance of the RWV strategy is updated to a nearly unmanned control manner with the example of methamphetamine and its aptamer. On the automatic RWV detection setup, the sensing surface acts similarly as before. The aptamer-based sensing surface resulted from the breakdown of the hydrophobic basis. The hydrophobicity is constructed on the metastable aptamer layer, which is responsive to the corresponding target. Methamphetamine interacts with its corresponding aptamer and destroys the basis of the hydrophobicity. A decrease in contact angle indicates the existence of methamphetamine. The RWV phenomenon is also affected by concentration and temperature. The development of an automatic detection ability would bring new possibilities to the surface reaction on smarter detection.

High Carbonization Temperature to Trigger Enzyme Mimicking Activities of Silk-Derived Nanosheets.

Herein, it is demonstrated that N-rich carbonized silk fibroin materials (CSFs) can serve as efficient peroxidase, and oxidase mimics. Their enzyme-like activities are highly dependent on carbonization conditions. CSFs obtained at low temperatures do not exhibit significant catalytic reactivity, while their enzyme-like catalysis performance is greatly activated after high-temperature treatment. Such a phenomenon is mainly ascribed to the increase of graphitization degree and graphitic nitrogen and the emergence of disordered graphitic structures during the formation of turbostratic carbon. In addition, inspired by the excellent photothermal conversion efficiency, and temperature-dependent catalytic behavior of CSFs, near-infrared light can be used to remotely control their enzyme-like activities. More importantly, as-prepared robust silk-derived nanosheets can be applied to photothermal-catalytic cancer therapy and sensing. It is believed that such a smart artificial enzyme system will throw up exciting new opportunities for the chemical industry and biotechnology.

Hot-Electron-Activated Peroxidase-Mimicking Activity of Ultrathin Pd Nanozymes.

Light-activated nanozymes can provide a wealth of new opportunities for the chemical industry and biotechnology. However, present remote-controlled catalytic systems are still far from satisfactory. Herein, we present an interesting example of applying ultrathin Pd nanosheets (Pd NSs) as a light-controllable peroxidase mimic. Since most of Pd atoms are exposed on their surface, Pd NSs with a thickness of 1.1 nm possess high peroxidase-like activity. More importantly, under light excitation, such intrinsic activity can be further activated by a nearly 2.4- to 3.2-fold. Such a phenomenon can be ascribed to the unique optical property of ultrathin Pd NSs, which can efficiently capture photons to generate hot electrons via surface plasmon resonance effect and thus promote the in situ decomposition of H2O2 into reactive oxygen species radicals (O*). This enhanced catalysis can also be used for real-time and highly sensitive colorimetric detection of H2O2. We expect our work can provide valuable insights into the rational design of artificial nanozymes with controllable and efficient activity in biomedical diagnostics, drug delivery, and environmental chemistry.

Wettability read-out strategy for aptamer target binding based on a recognition/hydrophobic bilayer surface.

We established a wettability read-out strategy for an aptasensor with a bilayer surface, using hydrophobicity reduction as the signal. The signal was induced by a fracture of the nanoneedle layer resulting from recognition by the supporting aptamer layer. The kinetics and influencing factors of this process were investigated with time curves.

Wool Keratin Photolithography as an Eco-Friendly Route to Fabricate Protein Microarchitectures.

Herein, we present an eco-friendly route to construct protein microarchitectures by using wool keratin (WK) as a green biophotoresist. The photoactive WK was extracted from wool with a method of alkaline hydrolysis and reacted with 2-isocyanatoethyl methacrylate (IEM). The whole process can avoid the use of surfactant and does not cause apparent changes in the structure and function of WK. With the aid of direct-write photolithography, "photoresist-like" WK can be further used for fabricating well-defined and high-performance patterns at a low micron-scale (µm) without high temperature, complicated process, long time, and high production cost.

Recent advances in nanoparticulate biomimetic catalysts for combating bacteria and biofilms.

Due to the abuse of antibiotics and the tendency of bacteria to form protective biofilms, the design and development of new efficient agents that can eliminate bacteria and biofilms are still highly desired but remain a great challenge; on the other hand, natural enzymes with unique catalytic characteristics can cause an irreversible damage to the bacteria without inducing drug-resistance in the bacteria. However, the intrinsic drawbacks, such as insufficient stability and high purification cost, of enzymes significantly limit their antimicrobial applications. Therefore, significant research efforts have been devoted towards the development of quality-equivalent or even superior enzyme substitutes with low cost and high stability. In this regard, nanomaterials with extraordinary enzyme-mimetic catalytic activities (termed as nanozymes) are considered as suitable candidates. To date, nanozymes have been proved to be promising materials for combating bacteria and biofilms under mild conditions. In this review, we have summarized the recent progress of nanozymes in this highly active field. The antibacterial mechanisms of nanozymes and the roles of their sizes, morphologies, compositions, surface modifications and microenvironment on their overall performance have been discussed. Moreover, the current challenges and prospects in this research area have been discussed. We believe that nanozymes with unique features and functions can provide a wealth of opportunities via their clinical and industrial applications.

Urea-Functionalized Poly(ionic liquid) Photonic Spheres for Visual Identification of Explosives with a Smartphone.

Current effort merging rational design of colorimetric sensor array with portable and easy-to-use hand-held readers delivers an effective and convenient method for on-site detection and discrimination of explosives. However, on the one hand, there are rare relevant reports; on the other hand, some limitations regarding direct sensing, color retention, and array extendibility still remain. Herein, urea-functionalized poly(ionic liquid) photonic spheres were employed to construct a brand-new colorimetric sensor array for directly identifying five nitroaromatic explosives with a smartphone. It is found that the strong hydrogen bonding between the urea motifs and the nitro groups offers the spheres high affinity for binding the targets, whereas the existence of other abundant intermolecular interactions in poly(ionic liquid) units renders one single sphere eligible for prominent cross-responses to a broad range of analytes. Besides, in our case, opal-like photonic crystal structures other than chemical dyes are used to fabricate a new style of colorimetric array. Such structural colors can be vivid and unchanged over a long period even in hazard environments. Importantly, through simply altering the preparation conditions of our PIL spheres, a pool of sensing elements could be added to the developed array for discrimination of extended target systems such as more explosives and even their mixtures in real-world context.

Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns.

The ability to pattern natural polymers at different scales is extremely important for many research areas, such as cell culture, regenerative medicine, bioelectronics, tissue engineering, degradable implants, and photonics. For the first time, the use of wool keratin (WK) as a structural biomaterial for fabricating precise protein microarchitectures is presented. Through straightforward biochemical processes, modified WK proteins become intrinsically photoreactive without significant changes in protein structure or function. Under light irradiation, intermolecular chemical crosslinking between WK molecules can be successfully initiated by using commercially available photoinitiators. As a result, high-performance WK patterning on the micrometer scale (µm) can be achieved through a combination of water-based photolithography techniques. By simply mixing with nanoparticles, enzymes, and other dopants, various "functional WK resists" can be generated. In addition, without the addition of any cell-adhesive ligands, these patterned protein microstructures are demonstrated as bio-friendly cellular substrates for the spatial guidance of cells on their surface. Furthermore, periodic microfabricated WK structures in complex patterns that display typical iridescent behavior can be designed and formed over macroscale areas (cm).

A nanoneedle-based reactional wettability variation sensor array for on-site detection of metal ions with a smartphone.

An enhancement of the reactional wettability variation (RWV) sensing strategy is achieved based on the wettability switch of a nanoneedle surface. The sensor unit is formed by coating hydrophobic azoimidazole compounds, as the responder compounds onto the originally hydrophilic surface of cobalt hydroxide nanoneedles. The complexation reaction between metal ions and azoimidazole ligands etches the hydrophobic coating and switches the surface wettability, making the surface hydrophilic again. This switch is revealed by a decrease in the static contact angle (CA) and an increase in the sliding angle of the surface. The reactivity is tuned by the derivatization and conformational manipulation of the azoimidazole compounds. A sensor array composed of six as-tuned sensor units is constructed to distinguish among the species and concentrations of Fe3+, Ni2+ and La3+ at a low limit of 10-6 M using the chemometric method of principal component analysis (PCA). In addition, a new on-site detection strategy is developed based on PCA of the sliding angle, which can be measured conveniently and swiftly with a smartphone app and a commercially available setup. The application of the general RWV strategy is envisioned to open new possibilities for on-site detection.

Remote activation of nanoparticulate biomimetic activity by light triggered pH-jump.

Herein, we report a facile, efficient and versatile method for the photo-regulation of pH-dependent activities of artificial enzymes by incorporating flash photolysis reagents. Under light excitation, a persistent pH shift is achieved by proton release from photosensitive 2-nitrobenzaldehyde. Following such change, the controlled activation of oxidase-like activity of nanoceria is successfully demonstrated.

Data analysis between controllable variables and the performance of CuS crackle based electrode.

In this article, we provide the data analysis between controllable variables and the performance of CuS crackle based electrode, there are four important factors which could influence the formation of cracks, the colloid concentration, drying temperature, colloid dosage and ambient humidity. We carried out and summed nineteen controlled data experiments below and other variates which could affect the performance were discussed in this article.

Highly Ordered and Multiple-Responsive Graphene Oxide/Azoimidazolium Surfactant Intercalation Hybrids: A Versatile Control Platform.

To produce graphene materials with better controllability, a new graphene oxide (GO) intercalation hybrid is fabricated with the incorporation and functionalization with the azoimidazolium (AzoIm+) surfactant. The hybrid exhibits a highly uniform lamellar structure in which a few layers of GO are stacked with AzoIm+ alternatively. Simultaneous control of the mesoscopic structures, aggregation properties, and electrochemical behavior of the hybrid is achieved by inheriting the photo, thermal, and mechanical responsiveness of azoimidazolium. Ultraviolet (UV) treatment produces a well-dispersed GO/AzoIm+ suspension aggregate and a precipitate, whereas the specific capacitance of the final hybrid decreases. The lamellar stacking becomes anisotropic by uniaxial stretching on a soft polymer. With a liquid crystal unit inserted between the layers, the d spacing of the lamella passes through transformation, disordering, and finally recovery stages, associated with the increasing and decreasing temperature. The explosive release of heat generated by the thermal reduction of GO is reduced in the GO/AzoIm+ intercalation hybrid. The release of heat is tunable by varying the relative quantity and UV treatment of AzoIm+. The physical properties of the hybrid allow the controlled preparation of ultrasmall Au nanodots between the GO layers. This represents a major step toward multiple-responsive integrated graphene applications.

Electrothermally Driven Fluorescence Switching by Liquid Crystal Elastomers Based On Dimensional Photonic Crystals.

In this article, the fabrication of an active organic-inorganic one-dimensional photonic crystal structure to offer electrothermal fluorescence switching is described. The film is obtained by spin-coating of liquid crystal elastomers (LCEs) and TiO2 nanoparticles alternatively. By utilizing the property of LCEs that can change their size and shape reversibly under external thermal stimulations, the λmax of the photonic band gap of these films is tuned by voltage through electrothermal conversion. The shifted photonic band gap further changes the matching degree between the photonic band gap of the film and the emission spectrum of organic dye mounting on the film. With rhodamine B as an example, the enhancement factor of its fluorescence emission is controlled by varying the matching degree. Thus, the fluorescence intensity is actively switched by voltage applied on the system, in a fast, adjustable, and reversible manner. The control chain of using the electrothermal stimulus to adjust fluorescence intensity via controlling the photonic band gap is proved by a scanning electron microscope (SEM) and UV-vis reflectance. This mechanism also corresponded to the results from the finite-difference time-domain (FDTD) simulation. The comprehensive usage of photonic crystals and liquid crystal elastomers opened a new possibility for active optical devices.

An efficient route to rapidly access silica materials with differently ordered mesostructures through counteranion exchange.

Control via exchange: By using a new type of imidazolium-based gemini surfactant as the template, highly ordered cubic mesoporous silica material (MCM-48) was directly synthesized in a broad range of reactant compositions and under mild conditions (see scheme). Moreover, by a simple counterion exchange strategy, hexagonal (MCM-41) and lamellar (MCM-50) mesoporous silicas are also easily and efficiently accessible.