Nucleocapsid-specific T cell responses associate with control of SARS-CoV-2 in the upper airways before seroconversion.

Despite intensive research since the emergence of SARS-CoV-2, it has remained unclear precisely which components of the early immune response protect against the development of severe COVID-19. Here, we perform a comprehensive immunogenetic and virologic analysis of nasopharyngeal and peripheral blood samples obtained during the acute phase of infection with SARS-CoV-2. We find that soluble and transcriptional markers of systemic inflammation peak during the first week after symptom onset and correlate directly with upper airways viral loads (UA-VLs), whereas the contemporaneous frequencies of circulating viral nucleocapsid (NC)-specific CD4+ and CD8+ T cells correlate inversely with various inflammatory markers and UA-VLs. In addition, we show that high frequencies of activated CD4+ and CD8+ T cells are present in acutely infected nasopharyngeal tissue, many of which express genes encoding various effector molecules, such as cytotoxic proteins and IFN-γ. The presence of IFNG mRNA-expressing CD4+ and CD8+ T cells in the infected epithelium is further linked with common patterns of gene expression among virus-susceptible target cells and better local control of SARS-CoV-2. Collectively, these results identify an immune correlate of protection against SARS-CoV-2, which could inform the development of more effective vaccines to combat the acute and chronic illnesses attributable to COVID-19.

Silver nanoparticles regulate Arabidopsis root growth by concentration-dependent modification of reactive oxygen species accumulation and cell division.

Silver nanoparticles (AgNPs) are widely used in industry, increasing their potential level in the environment. Plant root, the key organ absorbing water and nutrients, are directly exposed to the soil. Little is known about AgNP-mediated effects on plant root growth. Here, we show that AgNPs are absorbed by root and mostly localized in cell wall and intercellular spaces, which affect root growth in a dose-dependent manner. Increased root elongation was observed when Arabidopsis was exposed to an AgNP concentration of 50 mg L-1, while decreased elongation was observed at concentrations of equal to or more than 100 mg L-1. Similarly, there was an increase in the number of cells in the root apical meristem and also in cell-cycle related gene expression (CYCB1;1) at 50 mg L-1 AgNP, while both cell number and gene expression declined at concentrations equal to or more than 100 mg L-1. This indicates that AgNPs regulate root growth by affecting cell division. Reactive oxygen species (ROS) related genes were deferentially expressed after 50 mg L-1 AgNP treatment. Further studies showed that AgNPs induce ROS accumulation in root tips in a dose-dependent manner. KI treatment, which scavenges H2O2, partially rescued AgNP-inhibited root growth. The application 50 mg L-1 AgNPs also rescued the root length phenotype of upb1-1, a mutant with slightly higher ROS levels and longer root length. Our results revealed that ROS mediate the dose-dependent effects of AgNPs on root growth. These findings provide new insights into mechanisms underlying how AgNPs regulate root growth in Arabidopsis.