Rapid and sensitive detection of etomidate based on functionalized copper nanoclusters fluorescent probe.

Etomidate as a non-barbiturate sedative, has central inhibitory effect and addiction and has been listed as a controlled drug in some countries due to the abusing trend nowadays. Therefore, rapid and sensitive detection of etomidate is of great significance. In this work, a novel fluorescent sensing probe (CuNCs@MIPs) based on copper nanoclusters (CuNCs) and molecular imprinted polymers (MIPs) has been firstly reported. CuNCs was environment-friendly synthesized using poly(vinylpyrrolidone) as a template and ascorbic acid as a reducing agent. After functionalized with molecular imprinting technique, the CuNCs@MIPs probe has special binding cavities on surface to target etomidate, causing the fluorescence intensity rapidly decrease, which confirmed it has excellent sensitivity, selectivity and stability. Under optimal conditions, the fluorescent sensing probe presented high precision linear relationship for etomidate in range of 10-500 ng/ml with detection limit of 10 ng/ml, and the whole detection process was completed within 10 min. This sensing method has also been applied to real samples detection, still demonstrated excellent feasibility in electronic cigarette liquids and urine. More importantly, compared with previous methods, this fluorescent sensing method has advantages such as rapid, simple and easy to operate. Collectively, the proposed CuNCs@MIPs sensing probe has good fluorescence characteristics and simple synthesis strategy, showed a great potential in etomidate detection and application.

A tailored ratiometric fluorescent sensor based on CdTe and MgF2 quantum dots for trace N-ethylpentylone detection.

The development of low-cost and highly sensitive ratiometric fluorescence sensor, CdTe@MIPs/MgF2, for N-Ethylpentylone (NEP) detection in wastewater samples is described. In this system, CdTe@MIPs (λex = 370, λem = 570) are employed as the receptor and response unit for NEP, with MgF2 (λex = 370, λem = 470) as the reference signal to enhance stability. Under optimal conditions, the sensor shows fluorescent quenching response at 570 nm to NEP in linear range of 2-200 nM, with LOD of 0.6 nM. The sensor also demonstrates significant selectivity for NEP over other analogues and interferents, making it ideal for practical applications in wastewater analysis. This approach is potentially more cost-effective and sensitive than conventional mass spectrometry in detecting abused substances in sewage. Additionally, the MgF2 fluorescent nano-material was first-ever developed and investigated, which may be significant in future research.

Success rate of current human-derived gastric cancer organoids establishment and influencing factors: A systematic review and meta-analysis.

BACKGROUND: Human-derived gastric cancer organoids (GCOs) are widely used in gastric cancer research; however, the culture success rate is generally low. AIM: To explore the potential influencing factors, and the literature on successful culture rates of GCOs was reviewed using meta-analysis. METHODS: PubMed, Web of Science, and EMBASE were searched for studies. Two trained researchers selected the studies and extracted data. STATA 17.0 software was used for meta-analysis of the incidence of each outcome event. The adjusted Methodological Index for Non-Randomized Studies scale was used to assess the quality of the included studies. Funnel plots and Egger's test were used to detect publication bias. Subgroup analyses were conducted for sex, tissue source, histological classification, and the pathological tumor-node-metastasis (pTNM) cancer staging system. RESULTS: Eight studies with a pooled success rate of 66.6% were included. GCOs derived from women and men had success rates of 67% and 46.7%, respectively. GCOs from surgery or biopsy/endoscopic submucosal dissection showed success rates of 70.9% and 53.7%, respectively. GCOs of poorly-differentiated, moderately-differentiated and signet-ring cell cancer showed success rates of 64.6%, 31%, and 32.7%, respectively. GCOs with pTNM stages I-II and III-IV showed success rates of 38.3% and 65.2%, respectively. Y-27632 and non-Y-27632 use showed success rates of 58.2% and 70%, respectively. GCOs generated with collagenase were more successful than those constructed with Liberase TH and TrypLE (72.1% vs 71%, respectively). EDTA digestion showed a 50% lower success rate than other methods (P = 0.04). CONCLUSION: GCO establishment rate is low and varies by sex, tissue source, histological type, and pTNM stage. Omitting Y-27632, and using Liberase TH, TrypLE, or collagenase yields greater success than EDTA.

Bibliometrics analysis based on the Web of Science: Current trends and perspective of gastric organoid during 2010-2023.

BACKGROUND: Three-dimensional organoid culture systems have been established as a robust tool for elucidating mechanisms and performing drug efficacy testing. The use of gastric organoid models holds significant promise for advancing personalized medicine research. However, a comprehensive bibliometric review of this bur-geoning field has not yet been published. AIM: To analyze and understand the development, impact, and direction of gastric organoid research using bibliometric methods using data from the Web of Science Core Collection (WoSCC) database. METHODS: This analysis encompassed literature pertaining to gastric organoids published between 2010 and 2023, as indexed in the WoSCC. CiteSpace and VOSviewer were used to depict network maps illustrating collaborations among authors, institutions and keywords related to gastric organoid. Citation, co-citation, and burst analysis methodologies were applied to assess the impact and progress of research. RESULTS: A total of 656 relevant studies were evaluated. The majority of research was published in gastroenterology-focused journals. Globally, Yana Zavros, Hans Clevers, James M Wells, Sina Bartfeld, and Chen Zheng were the 5 most productive authors, while Hans Clevers, Huch Meritxell, Johan H van Es, Marc Van de Wetering, and Sato Toshiro were the foremost influential scientists in this area. Institutions from the University Medical Center Utrecht, Netherlands Institute for Developmental Biology (Utrecht), and University of Cincinnati (Cincinnati, OH, United States) made the most significant contributions. Currently, gastric organoids are used mainly in studies investigating gastric cancer (GC), Helicobacter pylori-infective gastritis, with a focus on the mechanisms of GC, and drug screening tests. CONCLUSION: Key focus areas of research using gastric organoids include unraveling disease mechanisms and enhancing drug screening techniques. Major contributions from renowned academic institutions highlight this field's dynamic growth.

Ultra-sensitive fluorescent detection of strychnine based on carbon dot self-assembled gold nanocage sensing probe.

The ultra-sensitive detection of strychnine is crucial to provide powerful evidence in strychnine poisoning cases. In this study, a novel fluorescent carbon dots (CDs) self-assembled gold nanocage (AuNCs) composite is synthesized for the ultra-sensitive detection of strychnine using molecularly imprinted polymer sensing technology (MIPs-CDs@AuNCs). With strong loading and delivery capability of AuNCs, the CDs could be loaded into AuNCs, where the anisotropy of CDs could significantly decrease and the fluorescence of the MIPs-CDs@AuNCs probe gained lower relative standard deviation (RSD). Moreover, the fluorescence response of MIPs-CDs@AuNCs to target strychnine was observed to be more significant than MIPs-CDs without gold nanocages. Under optimal conditions, the developed MIPs-CDs@AuNCs fluorescence strategy showed good linear relationship at the concentration of strychnine from 3 ng mL-1 to 200 ng mL-1 with the limit of detection as low as 1 ng mL-1. Besides, real blood samples were analyzed without complex pre-preparation procedure to investigate the performance of the proposed molecularly imprinted fluorescence probe, and satisfactory results were obtained with absolute deviations between -1.16 ng mL-1 and 1.28 ng mL-1, which exhibited a great potential for the detection of strychnine in health care work.

Trace detection of methcathinone in sewage using targeted extraction based on magnetic molecularly imprinted polymers coupled with liquid chromatography-tandem mass spectrometry.

Methcathinone, a new psychoactive substance (NPS), poses a serious threat to public health. Therefore, there is an urgent need to develop a reliable, selective, sensitive and simple analytical technique for monitoring trace amounts of this target NPS in complex matrices. For this purpose, magnetic molecularly imprinted polymers (MMIPs) based on MIPs combined with nano-sized magnetic Fe3O4 were developed for the specific enrichment of methcathinone in wastewater. The binding properties and selectivity of MMIPs toward methcathinone were evaluated and compared with non-imprinted polymer (MNIPs). For sensitive and selective extraction and determination of the target methcathinone, magnetic solid-phase extraction (MSPE) based on MMIPs was combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Under optimized conditions, the proposed method was successfully used for the detection of methcathinone in wastewater, which provided a low limit of detection of 0.3 ng L-1 and a limit of quantification of 1.0 ng L-1 with relative standard deviations of less than 6.89% for intra- and inter-day analyses. Good linearity in the range of 1-2000 ng L-1 with a coefficient of determination (R2) greater than 0.98 was observed. Moreover, a certified reference material of water sample was successfully analyzed with satisfactory results and the recoveries of spike experiments ranged from 96.35-116.7%.

New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA.

(1) Background: Salt stress is an abiotic factor that limits maize yield and quality. A highly salt-tolerance inbred AS5 and a salt-sensitive inbred NX420 collected from Ningxia Province, China, were used to identify new genes for modulating salt resistance in maize. (2) Methods: To understand the different molecular bases of salt tolerance in AS5 and NX420, we performed BSA-seq using an F2 population for two extreme bulks derived from the cross between AS5 and NX420. Transcriptomic analysis was also conducted for AS5 and NX420 at the seedling stage after treatment with 150 mM of NaCl for 14 days. (3) Results: AS5 had a higher biomass and lower Na+ content than NX420 in the seedling stage after treatment with 150 mM NaCl for 14 days. One hundred and six candidate regions for salt tolerance were mapped on all of the chromosomes through BSA-seq using F2 in an extreme population. Based on the polymorphisms identified between both parents, we detected 77 genes. A large number of differentially expressed genes (DEGs) at the seedling stage under salt stress between these two inbred lines were detected using transcriptome sequencing. GO analysis indicated that 925 and 686 genes were significantly enriched in the integral component of the membrane of AS5 and NX420, respectively. Among these results, two and four DEGs were identified as overlapping in these two inbred lines using BSA-seq and transcriptomic analysis, respectively. Two genes (Zm00001d053925 and Zm00001d037181) were detected in both AS5 and NX420; the transcription level of Zm00001d053925 was induced to be significantly higher in AS5 than in NX420 (41.99 times versus 6.06 times after 150 mM of NaCl treatment for 48 h), while the expression of Zm00001d037181 showed no significant difference upon salt treatment in both lines. The functional annotation of the new candidate genes showed that it was an unknown function protein. (4) Conclusions: Zm00001d053925 is a new functional gene responding to salt stress in the seedling stage, which provides an important genetic resource for salt-tolerant maize breeding.

Major depressive disorder is correlated with the mitochondrial ND1 T3394C mutation in two Han Chinese families: Two case reports.

BACKGROUND: Major depressive disorder (MDD) is the most frequent reason of disabled people in the world, as reported by the World Health Organization. However, the diagnosis of MDD is mainly based on clinical symptoms. CASE SUMMARY: The clinical, genetic, and molecular characteristics of two Chinese families with MDD are described in this study. There were variable ages of onset and severity in depression among the families. Both Chinese families had a very low pre-valence of MDD. The mitochondrial genomes of these pedigrees were sequenced and indicated a homoplasmic T3394C (Y30H) mutation, with the polymorphism located at a highly conserved tyrosine at position 30 of ND1. The analysis also revealed unique sets of mitochondrial DNA (mtDNA) polymorphisms orig-inating from haplogroups M9a3 and M9a. CONCLUSION: This finding of the T3394C mutation in two unrelated depressed patients provides strong evidence that this mutation may have a part in the etiology of MDD. However, In these two Chinese families having the T3394C mutation, no functional mtDNA mutation was observed. Therefore, T3394C mutations are related with MDD, and the phenotypic manifestation of these mutations may be affected by changes in nuclear genes or environmental factors.

Facile fabrication of robust bilayer film loaded with chitosan active microspheres for potential multifunctional food packing.

The utilization of microcarriers is an effective technique to protect and slow down the release of active ingredients, while the combination of microcarriers and film materials is an important way to expand the application scenario of active ingredients. The aim of this study was to develop a simple and facile strategy for designing a multifunctional bilayer bioactive film that combines stable mechanical properties, sustained-release characteristics for active ingredients with good antioxidant and antibacterial properties. The EGCG-loaded chitosan active microspheres were prepared by sol-gel method, and then the carboxymethyl cellulose solution containing the active microspheres was assembled onto the carboxymethyl chitosan gel substrate based on intermolecular hydrogen bonding to construct a film with a stable bilayer structure. The results indicated that the bilayer film had dense microstructure and excellent mechanical strength (37.05 MPa), and exhibited UV-blocking properties and excellent gas barrier performance. Meanwhile, the loading of active ingredients (EGCG) in the microspheres enabled the bilayer film to exhibit excellent antioxidant and antibacterial properties, and the controlled release of EGCG by the film was sustainable and showed pH responsiveness. The results of this work provide a new perspective for the design and development of bio-based active packaging film with tunable functional characteristics.

[Effect of Diurnal Temperature Range on the Number of Residents Hospitalized Due to Stroke in Lanzhou].

Objective To analyze the relationship between diurnal temperature range (DTR) and the hospitalization of stroke in Lanzhou,so as to provide a scientific basis for probing into the mechanism of temperature changes in inducing stroke and formulating comprehensive prevention and control measures for stroke by relevant departments.Methods The information of the patients hospitalized due to stroke in Lanzhou during January 2014 to December 2019 and the air pollutants (PM10,SO2,and NO2) and meteorological data in the same period were collected for statistical analysis.Spearman rank correlation analysis was performed to analyze the correlations between air pollutants and meteorological factors.The distributed lag nonlinear model was adopted to fit the relationship between DTR and the number of stroke inpatients,and three-dimensional diagrams and the correlation diagrams of DTR against stroke risk were established.The stratified analysis was performed according to gender and age (< 65 years and ≥65 years).Results From 2014 to 2019,a total of 92 812 stroke patients were hospitalized in Lanzhou,with a male-to-female ratio of 1.35:1.There was a nonlinear relationship between DTR and the number of stroke inpatients in Lanzhou,which presented a lag effect.The low DTR at 4.5 ℃ had the largest RR value of 1.25 (95%CI=1.16-1.35) for stroke inpatients at a cumulative lag of 18 d.The effect of high DTR (18.5 ℃) on the hospitalization of stroke patients peaked at a cumulative lag of 21 d,with an RR value of 1.09 (95%CI=1.01-1.18).The stratified analysis results suggested that low levels of DTR had greater effects on the hospitalization of male stroke patients and stroke patients <65 years.Conclusions Short-term exposure to different levels of DTR had an impact on the number of stroke inpatients,and low levels of DTR had a slightly greater impact on stroke inpatients than high levels of DTR.Importance should be attached to the protection of males and people aged <65 years at low levels of DTR.

Rhein inhibits endometriosis by targeting microRNA-135.

Endometriosis is a serious human gynecological disorder of women of reproductive age. The present study was designed to explore the therapeutic implications of rhein in the management of endometriosis. The results showed that rhein significantly (p<0.05) inhibited the proliferation of endometrial stroma cells in a dose-dependent fashion. Besides, the rhein treated endometrial stroma cells showed significantly (p<0.05) lower migration and invasion, in vitro. Transwell and wound healing assays showed that rhein also suppressed the migration and invasion of the endometrial stroma cells. Rhein was shown to target miR-135 at the molecular level to exert its anti-proliferative effects against the human endometrial stroma cells. Conversely, overexpression of miR-135 could nullify the anti-proliferative effects of rhein. Taken together, the findings of the present study highlight the therapeutic utility of rhein against human endometriosis. However, more in vivo studies are required.

Sensitive detection and primary metabolism analysis of flualprazolam in blood.

Flualprazolam, a new benzodiazepine psychoactive substance recently made available online, and outside the controlled substance list, is often used by criminals for rape and robbery. In this paper, flualprazolam was successfully identified by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) and nuclear magnetic resonance (NMR). Moreover, LC-Q-TOF-MS analysis method was proposed for the determination of flualprazolam in whole blood using the rabbit perfusion model. After metabolism analysis, a monohydroxylated metabolite 3-hydroxy-flualprazolam was found in the primary mass spectrum of metabolites. Meanwhile, the time effect curve of the flualprazolam in rabbit's blood was explored and the detection window was about 36 h. Moreover, the sensitivity of the established LC-Q-TOF-MS method was investigated with the limit of detection of 0.03 ng/mL. The successful analysis of an actual forensic case with this established method suggests that it might provide a reference method for drug detection or supervision in law enforcement agencies and identification institutions.

Highly sensitive fluorescent aptasensor based on MoS2 nanosheets for one-step determination of methamphetamine.

In this work, a simple and sensitive fluorescence aptasensor based on MoS2 nanosheets (MoS2-Ns) combined with a fluorophore-labeled aptamer (aptamer-FAM) for MTA determination in one step has been described. The aptamer-FAM can be spontaneously absorbed by the surface of MoS2-Ns to form an aptamer-FAM/MoS2-Ns' sensing platform, resulting in quenching of the fluorescence of aptamer-FAM largely. However, after introducing the target MTA, the fluorescence will be restored depending on the levels of MTA added. Such an above reaction platform possesses a linear correlation of between 5 and 2400 nM, with a detection limit of 2.3 nM (S/N = 3). Moreover, the cross reactivity to ketamine, morphine and cocaine was only slightly significant. Simultaneously, the assay was also successfully applied to recognize MTA in spiked human blood and urine, as well as in the real forensic identification samples obtained from a forensic case about a MTA abuser.

Recyclable Magnetic Fluorescence Sensor Based on Fe3O4 and Carbon Dots for Detection and Purification of Methcathinone in Sewage.

Sensitive, rapid, and low-cost detection of drug traces in sewage is very important for drug monitoring and control. In this study, a dual functional and recyclable magnetic fluorescent molecularly imprinted polymers (MFMIPs) sensor with high sensitivity for rapid detection and purification of methcathinone in sewage was developed. MFMIPs was prepared via molecular imprinting and conjugation with carbon dots as a fluorescent reporter on Fe3O4 (Fe3O4-MIPs@CDs). With strong recognition and adsorption toward methcathinone by the specific cavities on the surface of MFMIPs, the fluorescence of the sensor could dramatically be quenched once anchored with methcathinone. Under optimal conditions, the MFMIPs sensor presented high sensitivity with a linear range of 0.5-100 nM and a detection limit of 0.2 nM, which would be used to monitor drug prevalence and consumption within a certain region. This sensor was applied to the assay of methcathinone in sewage samples collected from Yuebeiyuan, Yanghu, and Xujiahu sewage pumping stations of Yuelu District. The calculated concentrations of methcathinone were 4.80, 15.33, and 8.59 nM in sewage samples, which were in good agreement with data tested by LC-MS/MS. For another function, MFMIPs exhibited purification toward methcathinone and the adsorption capacity was about 0.27 mg/g in a real sewage sample. Moreover, the sensor could be recycled and reused at least five times with the aid of an external magnetic field. Collectively, with good analytical performance and excellent recognition and selectivity to methcathinone, the proposed sensing system based on the magnetic core and molecularly imprinted polymers would open a door to establish highly sensitive and effective sensing systems for sewage analysis and purification.

Rapid detection of 17ß-estradiol based on shaddock peel derived fluorescent aptasensor for forensic examination.

17ß-estradiol (E2) detection technique had been shown to a potent method for identification of female blood in forensic practice since it was abundant in the healthy female body. Herein, we developed a fluorescent aptasensor based on carbon quatum dots (CQDs) derived from shaddock peel green synthesis for rapid detection of E2 as a useful auxiliary tool of forensic examination. The CQDs conjugated to the aptamer achieved fluorometric detection of E2 in blood and the blood of healthy female from 12 to 60 years old could be sensitive detected with the limit of detection of 0.025 ng/ml, and the analytical process could be completed within 10 min. The aptasensor was also used to assay E2 in forensic samples including blood and blood stain. In all instances, the results were positive when mixed samples involving female sample. This fluorescent aptasensor was proved to be a green, rapid and sensitive detection method of E2, and it exhibited great potential in discrimination of female samples in forensic practice.

Clinical application of repetitive transcranial magnetic stimulation for post-traumatic stress disorder: A literature review.

The efficacy of traditional treatment for post-traumatic stress disorder (PTSD) is still unsatisfactory. Repetitive transcranial magnetic stimulation (rTMS) has been widely used in the treatment of various types of mental disorders, including PTSD. Although rTMS has been demonstrated to be effective in many cases, there are still arguments regarding its mechanism and protocol. This review aims to summarize the origin, development, principle, and future direction of rTMS and introduce this neuro-stimulation therapy to relevant clinicians.

[Microbial Community Structure of Activated Sludge and its Response to Environmental Factors].

By mining and analyzing the published 16S rRNA amplification data of activated-sludge from 32 sewage and waste-water treatment facilities, at home and abroad, this study examines the microbial structure of sludge and its response to inflow water quality, temperature, and treatment type. Activated sludge generally shows high species diversity and community richness. Many activated sludge samples contain different microbial community structures. In these samples, the dominant bacteria included Thauera, Nitrospira, Comamonas, Dechloromonas, Rhodoferax, Aquihabitans, and Acidovorax. Temperature was negatively correlated with several key denitrifying microorganisms, such as Nitrospira, Aquihabitans, Terrimonas, and Dechloromona. When temperatures were lower than 15℃, the corresponding removal rates of TN and NH4+-N in the 32 sewage treatment plants only reached 49.67% and 63.19%, respectively. With higher BOD5/COD values, these sewage treatment systems exhibited improved biodegradability performances. With higher relative abundances of advantageous functional bacteria, such as Zoogloea, Arcobacter, Acidovorax, and Acinetobacter, pollutant-removal rates would increase accordingly. As for domestic-sewage treatments, the performance of the A2O process is superior to that of the OD, CAS, and CMAS processes. Additionally, the relative abundances of multiple functional dominant bacteria, including Comamonas, Rhodoferax, Nitrospira, and Novosphingobium, were significantly higher in sludge treated with the A2O process than in sludge treated with the other three processes.

HCV core antigen plays an important role in the fight against HCV as an alternative to HCV-RNA detection.

OBJECTIVE: To discuss the clinical significance of HCV-cAg testing in the diagnosis, activity determination, and monitoring of therapeutic effectiveness of HCV infection and its advantages compared with HCV-RNA and anti-HCV antibodies detection. METHODS: By summarizing the published literature, the advantages and significance of HCV core antigen detection were sought. RESULTS: The expression of HCV-cAg is highly consistent with that of HCV-RNA, but compared with HCV-RNA, detection of HCV-cAg is easy to operate, time saving, and low cost. HCV-cAg can be detected within 12~15 days after infection, and the window period can be shortened by5~7 weeks. HCV-cAg is a serological indicator of virus replication, which can distinguish previous infection of HCV or current infection. HCV-cAg detection is more suitable for immunocompromised, hemodialysis, organ transplant patients. HCV-cAg also can be used to monitor antiviral efficacy and predict sustained virological response (SVR). CONCLUSION: HCV core antigen has similar clinical sensitivity to NAT and can be used as a substitute for HCV-RNA in the diagnosis of virus infection. Combined detection of HCV-cAg and antibody serology can help doctors detect HCV infection earlier, accurately diagnose different stages of HCV infection, and evaluate the therapeutic effect of antiviral drugs, which are beneficial in the prevention and treatment of hepatitis C.

The psychological impact of COVID-19 on the families of first-line rescuers.

Under the COVID-19 epidemic, the families of front-line rescue workers are under unusual pressure. We aim to understand the extent of their psychological distress in this epidemic and whether they have received sufficient support. Thus targeted to provide support for them and indirectly reduce the concerns of the rescue workers. From February 27 to March 1, 2020, we used the scales of Perceived Stress Scale, 10-items Connor-Davidson Resilience Scale, Generalized Anxiety Disorder-7, Patient Health Questionnaire-2, and primary care-posttraumatic stress disorder (PTSD) to evaluate the mental health level of the family members of front-line rescue workers online. Six hundred and seventy one family members voluntarily filled out the questionnaire after reading the purpose of the study. A total of 671 family members of front-line rescue workers participated in the survey, including 194 husbands/wives, 52 parents, 49 children, 76 brothers and sisters, and 300 other relationships. Among them, 55% of the family members reported sleep problems, 49.0% of the family members had mild, and above anxiety symptoms, 12.2% of the family members reported clinically significant depression symptoms, and 10.4% of the family members may have PTSD, 8.3% of family members had thoughts of self-injury or suicide. The feeling of stress was positively correlated with anxiety and depression, but there was no significant correlation between psychological elasticity and various symptoms. Family members who are more worried about the safety, physical condition and living security of front-line rescue workers are more likely to report symptoms such as sleep disorders, anxiety, and depression. Family members' first-line rescue has a significant impact on their daily life, raising children and supporting the elderly, which is also related to the occurrence of mental and psychological symptoms. During the period of front-line support, they received support from the units or organizations of the first-line workers, mainly including telephone greetings and daily necessities. The top three concerns about the information of the epidemic were the time of the end of the epidemic, the time of the return of the family and the progress of COVID-19's treatment. After the end of the epidemic, what they most want to do is to pay more attention to and accompany their families, family gatherings, and do more exercise. The psychological impact of this epidemic on the families of front-line rescue workers is mainly sleep problems and anxiety. The psychological intervention of family members should mainly start from the relief of stress and increase of material and spiritual support.

Psychometric property study of the posttraumatic stress disorder checklist for DSM-5 (PCL-5) in Chinese healthcare workers during the outbreak of corona virus disease 2019.

BACKGROUND: Previous studies about the reliability and validity of the updated PCL version for the fifth edition of the Diagnostic and Statistical Manual for Mental Disorders (PCL-5) have only been evaluated in certain samples of the population, which lacks in the sample of Healthcare Workers. Our study focused on the factor structure, reliability and validity of the PCL-5 among Chinese Healthcare Workers during the Outbreak of Corona Virus Disease 2019. METHODS: We conducted an online survey of frontline healthcare workers using the PCL-5 for PTSD. Total of 212 frontline healthcare providers were included in this study. RESULTS: The findings showed that PCL-5 is a reliable instrument in our sample. The total and subscale scores showed good internal consistency. The convergent and discriminant validity of the PCL-5 were also well demonstrated. Our result showed a better fit with the seven-factor hybrid model compared with other models and supported that the PCL-5 Chinese version can be used as a reliable screening tool to conduct psychological screening for Chinese healthcare workers. LIMITATION: We could not examine other aspects of reliability and validity like test-retest reliability or criterion validity. We didn't use the gold-standard structured interview for PTSD in our study. Besides, most of our samples were young people who had access to the internet. Not all professional levels and seniorities were presented because our sample had a lower mean income and educational level. CONCLUSION: Our study shows that the Chinese PCL-5 has good validity and reliability in frontline healthcare workers during the outbreak.