ON-SITE monitoring OF BVOCS emission in Tremiti island, Italy.

A measurement campaign was conducted on San Domino Island, part of the Tremiti Islands archipelago, located in Foggia, Italy. The area is almost entirely covered by vegetation, dominated by the following main species: Juniperus turbinata, Helichrysum italicum, Myrtus communis, Rosmarinus officinalis, Pistacia lentiscus and Pinus halepensis.This study focused on the BVOCs emitted by plants and the ground, employing a simple, economical, and efficient sampling and analysis method. The main known BVOC species emitted by Mediterranean plant species as α-pinene, ß-pinene, camphene and limonene were detected. The measurements highlighted a daily complementarity between plant and soil emissions. The daily variations in BVOCs emitted by both plants and the soil are differ, ensuring an almost constant concentration throughout the day. At the same time, the composition of sea spray aerosol (SSA) was also measured. The measurement sites were selected based on botanical characterization to account for the predominant species on San Domino Island, and the sampling was conducted at human height to accurately identify the species for potential use. The combination of beneficial effects of the substances emitted by plant species and soil, along with the simultaneous presence of SSA, are factors that could enhance the effectiveness of forest therapy in a previously unexplored location.

A high-precision jujube disease spot detection based on SSD during the sorting process.

The development of automated grading equipment requires achieving high throughput and precise detection of disease spots on jujubes. However, the current algorithms are inadequate in accomplishing these objectives due to their high density, varying sizes and shapes, and limited location information regarding disease spots on jujubes. This paper proposes a method called JujubeSSD, to boost the precision of identifying disease spots in jujubes based on a single shot multi-box detector (SSD) network. In this study, a diverse dataset comprising disease spots of varied sizes and shapes, varying densities, and multiple location details on jujubes was created through artificial collection and data augmentation. The parameter information obtained from transfer learning into the backbone feature extraction network of the SSD model, which reduced the time of spot detection to 0.14 s. To enhance the learning of target detail features and improve the recognition of weak information, the traditional convolution layer was replaced with deformable convolutional networks (DCNs). Furthermore, to address the challenge of varying sizes and shapes of disease spot regions on jujubes, the path aggregation feature pyramid network (PAFPN) and balanced feature pyramid (BFP) were integrated into the SSD network. Experimental results demonstrate that the mean average precision at the IoU (intersection over union) threshold of 0.5 (mAP@0.5) of JujubeSSD reached 97.1%, representing an improvement of approximately 6.35% compared to the original algorithm. When compared to existing algorithms, such as YOLOv5 and Faster R-CNN, the improvements in mAP@0.5 were 16.84% and 8.61%, respectively. Therefore, the proposed method for detecting jujube disease spot achieves superior performance in jujube surface disease detection and meets the requirements for practical application in agricultural production.

Improved Thermal Insulation and Mechanical Strength of Styrene-Butadiene Rubber through the Combination of Filled Silica Aerogels and Modified Glass Fiber.

To improve heat dissipation capability and enhance mechanical properties, a series of silica aerogel (SA) and modified glass fiber (GF)-filled SBR composites were prepared. It was found that the addition of SA successfully reduced the thermal conductivity of SBR by 35%, owing to the heat shield of the nanoscale porous structure of SA. Moreover, the addition of modified glass fiber (MGF) yielded a significant increase in the tensile and tear strength of SBR/SA composite rubber of 37% and 15%, respectively. This enhancement was more pronounced than the improvement observed with unmodified GF, and was attributed to the improved dispersion of fillers and crosslinking density of the SBR matrix. Rheological analysis revealed that the addition of SA and MGF weakened the ω dependence. This was due to the partial relaxation of immobilized rubber chains and limited relaxation of rubber chains adsorbed on the MGF. Furthermore, the strain amplification effect of MGF was stronger than that of GF, leading to a more pronounced reinforcing effect.

Mechanisms of lead uptake and accumulation in wheat grains based on atmospheric deposition-soil sources.

Lead (Pb) accumulation in wheat grains depends on two aspects: i) Pb uptake by the roots and shoots, and ii) the translocation of organ Pb into the grain. However, the underlying mechanism of the uptake and transport of Pb in wheat remains unclear. This study explored this mechanism by establishing field leaf-cutting comparison treatments. Interestingly, as the organ with the highest Pb concentration, only 20.40 % of the root's relative contribution to grain Pb. The relative contributions of the spike, flag leaf, second leaf, and third leaf to grain Pb were 33.13 %, 23.57 %, 13.21 %, and 9.69 %, respectively, which was opposite to their Pb concentration distribution trends. According to Pb isotope analysis, it was found leaf-cutting treatments reduced the proportion of atmospheric Pb in grain, and grain Pb predominantly comes from atmospheric deposition (79.60 %). Furthermore, from the bottom to the top, the concentration of Pb in internodes decreased gradually, and the proportions of Pb originating from soil in the nodes also decreased, revealing that wheat nodes hindered the translocation of Pb from roots and leaves to the grain. Therefore, the hindering effect of nodes on the migration of soil Pb in wheat resulted in atmospheric Pb having a more convenient pathway to the grain than soil Pb, and further leading grain Pb accumulation primarily depended on the contribution of the flag leaf and spike.

Influence mechanism of awns on wheat grain Pb absorption: Awns' significant contribution to grain Pb was mainly originated from their direct absorption of atmospheric Pb at the late grain-filling stage.

The spike is the organ that contributes the most to lead (Pb) accumulation in wheat grains. However, as an important photosynthetic and transpiration tissue in spike, the role of awn in wheat grain Pb absorption remains unknown. A field experiment was conducted to investigate the influence mechanism of awn on grain Pb accumulation through two comparative treatments: with and without awns (de-awned treatment). The de-awned treatment decreased wheat yield by 4.1 %; however, it significantly lowered the grain Pb accumulation rate at the late filling stage (15 days after anthesis) and led to a 22.8 % decrease in grain Pb concentration from 0.57 to 0.44 mg·kg-1. Moreover, the relative contribution of awn-to-grain Pb accumulation gradually increased with the filling process, finally reaching 26.6 % at maturity. In addition, Pb isotope source analysis indicated that the Pb in the awn and grain mainly originated from atmospheric deposition, and the de-awned treatment decreased the proportion of grain Pb from atmospheric deposition by 8.9 %. Microstructural observations further confirmed that the contribution of awns to grain Pb accumulation mainly originated from their direct absorption of atmospheric Pb. In conclusion, awns play an important role in wheat grain Pb absorption at the late grain-filling stage; planting awnless or short-awn wheat varieties may be the simplest and effective environmental management measure to reduce the health risks of Pb in wheat in regions with serious atmospheric Pb contamination.

The newly absorbed atmospheric lead by wheat spike during filling stage is the primary reason for grain lead pollution.

Wheat spikes could directly absorb lead (Pb) from atmospheric depositions. However, the mechanism by which the spikes contribute to Pb accumulation in the grain remains unclear. To investigate this mechanism, a field experiment was conducted using three comparative spikes shading treatments: 1) exposed to atmospheric deposition and light (CK), 2) non-exposed to atmospheric deposition and light (T1), and 3) non-exposed to atmospheric deposition but light-transmitting (T2). Spikes shading treatments reduced the average rate and peak value of the accumulation of Pb in grains, which significantly decreased the grain Pb concentration by 57.44 % and 50.26 % in T1 and T2 treatments, respectively. Moreover, Pb isotopic analysis shows that the Pb in spike and grain was mainly from atmospheric deposition, and the percentage of the grain Pb originated from atmospheric Pb decreased from 85.98 % in CK to 72.87 % and 79.59 % in T1 and T2, respectively. In addition, the spikes, rather than the leaves/roots, were the largest wheat tissue source of Pb in grains, and the relative contribution of spikes to grain Pb accumulation increased to 65.57 % at the maturity stage, of which the stored Pb re-translocation of spikes and the newly absorbed Pb by spikes during the filling stage contributed 13.37 % and 52.20 % to the grain Pb, respectively. Thus, the contribution of the spike to the grain Pb was mainly from the newly absorbed Pb from the atmospheric deposition during the grain filling phase, and grain filling phase is the key stage for the absorption of Pb by the grain. In brief, the newly absorbed atmospheric Pb by wheat spike during filling stage is the primary cause of grain Pb contamination, which provided a new insight for effective control of wheat Pb pollution.

Health utility measurement for people living with HIV/AIDS under combined antiretroviral therapy: A comparison of EQ-5D-5L and SF-6D.

We compared the discriminative validity, agreement and sensitivity of EQ-5D-5L and SF-6D utility scores in people living with HIV/AIDS (PLWHIV). We conducted a cross-sectional survey among PLWHIV aged more than 18 years old in 9 municipalities in Yunnan Province, China. A convenience sample was enrolled. We administered the SF-12 and EQ-5D-5L to measure health-related quality of life. The utility index of the SF-6D was derived from the SF-12. We calculate correlation coefficients to evaluate the relationship and agreement of 2 instruments. To evaluate the homogeneity of the EQ-5D-5L and SF-6D, intraclass correlation coefficients, scatter plots, and Bland-Altman plots were computed and drawn. We also used receiver operating characteristic curves to compare the discriminative properties and sensitivity of the econometric index. A total of 1797 respondents, with a mean age of 45.6 ±â€…11.7 years, was interviewed. The distribution of EQ-5D-5L scores skewed towards full health with a skewness of -3.316. The overall correlation between EQ-5D-5L and SF-6D index scores was 0.46 (P < .001). The association of the 2 scales appeared stronger at the upper end. An intraclass correlation coefficient of 0.59 between the EQ-5D-5L and SF-6D meant a moderate correlation and indicated general agreement. The Bland-Altman plot displayed the same results as the scatter plot. The receiver operating characteristic curve showed that the AUC for the SF-6D was 0.776 (95% CI: 0.757, 0.796) and that for the EQ-5D-5L was 0.732 (95% CI: 0.712, 0.752) by the PCS-12, and it was 0.782 (95% CI: 0.763, 0.802) for the SF-6D and 0.690 (95% CI: 0.669, 0.711) for the EQ-5D-5L by the MCS-12. Our study demonstrated evidence of the performance of EQ-5D-5L and SF-6D index scores to measure health utility in people living with HIV/AIDS. There were significant differences in their performance. We preferred to apply the SF-6D to measure the health utility of PLWHIV during the combined antiretroviral therapy period. Our study has demonstrated evidence for instrument choice and preference measurements in PLWHIV under combined antiretroviral therapy.

A five-year review of prevalence and treatment outcomes of pre-extensively drug-resistant plus additional drug-resistant tuberculosis in the Henan Provincial Tuberculosis Clinical Medicine Research Centre.

OBJECTIVES: This study investigated the prevalence and significant clinical outcomes of pre-extensively drug-resistant plus additional drug-resistant tuberculosis (pre-XDR-plus) in Henan Provincial Chest Hospital between 2017 and 2021. METHODS: We analysed and summarized the drug sensitivity test (DST) results of clinical Mycobacterium tuberculosis (MTB) strains in TB patients seeking care in the Tuberculosis Clinical Medical Research Centre of Henan Province between 2017 and 2021. Medical records of pre-extensively drug-resistant plus additional drug-resistant TB patients were statistically analysed, including demographic characteristics, regimens, and outcomes. RESULTS: Of the 3689 Mycobacterium tuberculosis strains, 639 (17.32%), 353 (9.56%), and 109 (2.95%), multidrug-resistant tuberculosis (MDR-TB), pre-extensively drug-resistant tuberculosis (pre-XDR), and pre-XDR-plus, respectively. The proportion of MDR decreased from 19.1% in 2017 to 17.5% in 2021 (χ2 = 0.686, P = 0.407), the proportion of pre-XDR from 11.4% in 2017 to 9.0% in 2021 (χ2 = 2.39, P = 0.122), and pre-XDR-plus from 4.7% in 2017 to 1.8% in 2020, with the declining trend was significant (χ2 = 9.348, P = 0.002). The most commonly used anti-TB drugs were pyrazinamide (PZA, 37/46, 80.43%) and cycloserine (CS, 32/46, 69.57%), followed by linezolid (LZD, 25/46, 54.35%), protionamide (TH, 25/46, 54.35%), and para-aminosalicylic acid (PAS, 23/46, 50.00%). Patients receiving the LZD regimen were 5 times more likely to have a favourable outcome than those not receiving LZD (OR = 6.421, 95% CI 2.101-19.625, P = 0.001). Patients receiving a regimen containing CS were 4 times more likely to have a favourable outcome compared to those not taking CS (OR = 5.444, 95% CI 1.650-17.926, P = 0.005). CONCLUSIONS: Our data suggest that the population of pre-XDR-plus had significantly decreased over the past five years in the Henan Provincial Chest Hospital. The COVID-19 and flood disaster affect TB patients' selection of medical services. In addition, the pre-XDR-plus patients whose regimens contain LZD or CS were more likely to have favourable outcomes.

CAMTA1 gene affects the ischemia-reperfusion injury by regulating CCND1.

Epigenetic modulations lead to changes in gene expression, including DNA methylation, histone modifications, and noncoding RNAs. In recent years, epigenetic modifications have been related to the pathogenesis of different types of cancer, cardiovascular disease, and other diseases. Emerging evidence indicates that DNA methylation could be associated with ischemic stroke (IS) and plays a role in pathological progression, but the underlying mechanism has not yet been fully understood. In this study, we used human methylation 850K BeadChip to analyze the differences in gene methylation status in the peripheral blood samples from two groups (3 IS patients vs. 3 healthy controls). According to their bioinformatics profiling, we found 278 genes with significantly different methylation levels. Seven genes with the most significant methylation modifications were validated in two expanded groups (100 IS patients vs. 100 healthy controls). The CAMTA1 gene had significantly different methylation changes in patients compared to the controls. To understand the CAMTA1 function in stroke, we generated CAMTA1 knockout in SH-SY5Y cells. RNA seq results in CAMTA1 knockout cells revealed the pathways and gene set enrichments involved in cellular proliferation and cell cycle. Furthermore, a series of experiments demonstrated that in the oxygen-glucose deprivation/re-oxygenation (OGD/R) model system, the expression of cyclin D1, an essential regulator of cell cycle progression, was increased in SH-SY5Y CAMTA1 KO cells. Increasing evidence demonstrated that ischemic stress could inappropriately raise cyclin D1 levels in mature neurons. However, the molecular signals leading to an increased cyclin D1 level are unclear. Our findings demonstrate for the first time that the CAMTA1 gene could regulate cyclin D1 expression and implicate their role in strokes.

Remediation of Pb-Contaminated Soil Using a Novel Magnetic Nanomaterial Immobilization Agent.

The management of heavy metal contaminated soil has received extensive research attention. In this study, a novel immobilization agent (SiO2@Fe3O4@C-COOH) was combined with traditional immobilization agents (TIAs), i.e., CaO, organic matter (OM), and calcium superphosphate (CSP), and used to remediate Pb-contaminated soil. The immobilization effects of Pb in soil was evaluated through pot experiments involving wheat cultivation. The results indicated that SiO2@Fe3O4@C-COOH delivered a higher Pb immobilization efficiency than did TIAs such as CaO, OM, and CSP. The application of SiO2@Fe3O4@C-COOH in combination with TIAs (CaO, OM, and CSP) synergistically enhanced the Pb immobilization efficiency of the soil to 85.10%. Further, joint application in a 54.19% reduction of Pb content in wheat roots, a 65.78% reduction in stems, and a 47.96% in leaves. Thus, the combined application of SiO2@Fe3O4@C-COOH and TIAs significantly reduced the bioavailability of Pb, achieved the purpose of Pb stabilization and soil remediation, and has the potential for wide-spread application in the remediation of Pb-contaminated soils.

Relative contribution of environmental medium and internal organs to lead accumulation of wheat grain.

Lead (Pb) pollution in wheat has received considerable research attention globally due to its persistence and ease of accumulation, posing severe health risks to humans. This study explored the relative contribution of the environmental medium (atmospheric deposition and soil) and wheat internal organs to Pb accumulation in wheat grains, using field experiments by contrasting treatments. The concentration and bioavailability of Pb in the soil were significantly lower than those of atmospherically deposited Pb (P < 0.05). Pb accumulation rate in wheat grains was consistent with the grain filling rate, which first increased and then decreased, reaching the highest level at the middle filling stage. Pb isotope analysis showed that atmospheric deposition was the main source of Pb in the shoots of wheat plants, contributing more than 80.0% of Pb in grains. Although the roots had the highest Pb concentration, the spikes had the greatest relative contribution (58.4%) to Pb accumulation in the wheat grains, followed by that of the leaves (24.5%), whereas the contribution of roots was the lowest (17.1%) among all plant organs. In addition, among all leaves, the contribution of flag leaves to Pb accumulation in the grain was higher than the cumulative contribution of all other leaves, where flag leaves and other leaves contributed 13.8% and 10.7%, respectively. Collectively, the absorption of atmospherically deposited Pb by wheat spikes is the leading cause of Pb pollution in wheat grains. These results may aid in formulating strategies to reduce Pb concentration in grains and ensure food quality and safety.

Preparation and Finite Element Analysis of Fly Ash/HDPE Composites for Large Diameter Bellows.

In recent years, buried bellows have often had safety accidents such as pipeline bursts and ground subsidence due to the lack of adequate mechanical properties and other quality problems. In order to improve the mechanical properties of bellows, fly ash (FA) was used as a reinforced filler in high density polyethylene (HDPE) to develop composites. The FA was surface treated with a silane coupling agent and HDPE-g-maleic anhydride was used as compatibilizer. Dumbbell-shaped samples were prepared via extrusion blending and injection molding. The cross-section morphology, thermal stability and mechanical properties of the composites were studied. It was observed that when 10% modified FA and 5% compatibilizer were added to HDPE, the tensile yield strength and tensile breaking strength of the composites were nearly 30.2% and 40.4% higher than those of pure HDPE, respectively, and the Young's modulus could reach 1451.07 MPa. In addition, the ring stiffness of the bellows was analyzed using finite element analysis. Compared with a same-diameter bellows fabricated from common commercially available materials, the ring stiffness increased by nearly 23%. The preparation method of FA/HDPE is simple, efficient, and low-cost. It is of great significance for the popularization of high-performance bellows and the high value-added utilization of FA.

Contribution of the flag leaf to lead absorption in wheat grain at the grain-filling stage.

Wheat flag leaf (FL) is one of the primary sources of carbohydrates in grains; however, its role in grain lead (Pb) absorption remains unclear. A field experiment was conducted to assess the relative contribution of the FL to Pb accumulation in wheat grain by two contrasting treatments: without (CK) and with FL removal (FLR) at the grain-filling stage. The Pb concentration in leaves was closely related to leaf strata and decreased from FL to the third leaf. FLR treatment significantly reduced the yield and grain Pb concentration by 2.79% and 11.47%, respectively. The contribution of FL to grain Pb accumulation decreased gradually with the filling process, from 35.08% (at early stage) to 13.94% (at maturity stage). After FLR, the contribution proportion of atmospheric fallout to grain Pb decreased from 69.01% (CK) to 62.43% (FLR). Combined isotope analysis with scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM-EDS) revealed that the main contribution of FLs to grain Pb originated from Pb fallout in fine atmospheric particles. Therefore, taking measures to reduce the influence of fine atmospheric particles on wheat may be an effective way to control wheat grain Pb contamination.

The role of personality, social economic and prevention strategy effects on health-related quality of life among people living with HIV/AIDS.

BACKGROUND: HIV/AIDS has transformed into a chronic controllable but not yet curable infectious disease as other chronic diseases to some extent. The additional of so called fourth 90% that included the improved health-related quality of life (HRQoL) for people living with HIV (PLWHIV) required solutions beyond antiretroviral therapy and viral load suppression. This study will explore the role of personality, social economic and prevention strategy effection on HRQoL among people living with HIV/AIDS. METHODS: A cross-sectional study was conducted among PLWHIV aged more than 16 years old in the 10 municipalities in Yunnan Province, China from October 2019 to May 2020, enrolling total 1997 participants. Individual-level HRQoL data were measured by 12-item Short Form Health Survey (SF-12) and EuroQol Five Dimensions Questionnaire (EQ-5D-5L). We assembled municipal-level data about social economic from Yunnan Statistical Yearbook in 2020 and strategy practice information from the self-evaluation system. We used the principal component analysis to build the social economic and strategy effect on each area respectively and one-way ANOVA was used to perform univariate analysis to identify the predictors with significant differences. Finally we used multi-level model (MLM) to explore the personality, social economic and strategy effects in health-related quality of life among PLWHIV. RESULTS: The global score for quality of life measured using EQ-5D-5L had an estimated mean score (standard deviation, SD) of 0.901 ± 0.146. The HRQoL score measured using PCS-12 had an estimated mean score (SD) of 46.62 ± 8.55. The mean MCS-12 score (SD) was estimated to be 47.80 ± 9.71. The area-level predictors explained a proportion of 13.6-17.2% for the between-area variation of the HRQoL scores, regardless of the total HRQoL, physical component and mental component. The impacts of stigma (P < 0.01), social support (P < 0.001), anxiety (P < 0.001), depression (P < 0.05) and social economic status (P < 0.05) on HRQoL at the individual-level were significantly different. The plots visualized the impact of individual-level factors on a respondent's HRQoL was modified by the area-level characteristics. CONCLUSIONS: The study identified the possible strategy determinant of individual HRQoL of PLWHIV and also the area effect on HRQoL. Stigma, social support, anxiety, depression and social economic status were the individual-level determinants on HRQoL. These could be a valuable resource for evaluating the overall health of the areas and help improve local decision making.

Mechanism of Pb absorption in wheat grains.

Atmospheric deposition is the primary source of external environmental media for lead (Pb) influx in wheat grains. However, the mechanisms of Pb grain absorption remains unclear. We explored this mechanism through comparative experiments, involving defoliating leaf blades (TG) and a control group (CK) of field wheat after the anthesis stage. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy analysis displayed that leaves and ears can directly absorb atmospheric deposition Pb through stomata. Compared with CK, the yield, grain Pb content, and grain Pb accumulation of TG wheat were significantly decreased by 13.25%, 22.10%, and 32.58%, respectively. Combined with the Pb isotope analysis, the ear had the highest contribution to grain Pb followed by leaf and root. Simultaneously, the absorption rate of grain Pb demonstrated a dynamic trend of "N" shape. Dominant contribution periods of the root, leaf, and ear organs to grain Pb accumulation were different. Unlike the root system, the contribution of the aboveground to grain Pb increased gradually, and the contribution of leaf and ear to grain Pb were mainly concentrated in the early and late filling stage, respectively. Our findings can provide a theoretical basis for the control of Pb pollution in grains.

A Promoter Polymorphism (Rs57137919) of ABCG1 Gene Influence on Blood Lipoprotein in Chinese Han Population.

BACKGROUND: Adenosine triphosphate-binding cassette subfamily G member 1 (ABCG1) has the function of transporting free intracellular cholesterol to extracellular high-density lipoprotein (HDL) particles, which play a crucial role in atherosclerosis. The goal of this study is to examine the relationship between the polymorphisms of the ABCG1 gene promoter region and ischemic stroke. METHODS: In the present study, a case-control association study was designed to identify 3 single-nucleotide polymorphisms (SNPs; rs5713919, rs1378577, and rs1893590), which were located in the promoter region of ABCG1 gene by kompetitive allele-specific polymerase chain reaction genotyping approach. The in vitro luciferase assay was done to estimate the effect of rs5713919 on gene expression. Finally, the relationships of 3 SNPs of ABCG1 gene with plasma lipids and lipoproteins were investigated in this Chinese cohort. RESULTS: The correlation analysis between lipids and genotypes showed that the rs57137919 locus genotype was significantly associated with HDL cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels (P = 0.021 and P = 0.017, respectively), and the GA and AA genotypes had higher HDL-C levels than the GG genotype. CONCLUSIONS: Our study provides evidence that ABCG1 promoter region polymorphism rs57137919 has an influence on plasma HDL-C and LDL-C levels in Chinese Han population.

A c.544_618del75bp mutation in the splicing factor gene PRPF31 is involved in non-syndromic retinitis pigmentosa by reducing the level of mRNA expression.

PURPOSE: A previous study reported a novel c.544_618del75bp mutation in exon 7 of the PRPF31 gene in a Chinese family with autosomal dominant retinal pigmentosa (ADRP). However, the selected pedigree was a small part of the whole family and the function of the c.544_618del75bp mutation was not explored deeply. The aim of the present study was to validate the previous results and explore the functional significance of the c.544_618del75bp mutation. METHODS: We extended the size of the ADRP pedigree and sequenced DNA and cDNA of the PRPF31 gene for all members of the family and 100 healthy controls. Real-time quantitative polymerase chain reaction (PCR) analysis was performed on the cDNA of patients in the family and cell culture, plasmids transfection and western blot analysis were done to evaluate the functional effect of the mutation in vitro. RESULTS: Sanger sequencing showed that the mutation was present in all patients and absent in all normal individuals, except for participant III-9. Bioinformatics analysis revealed that the c.544_618del75bp mutation caused a 25 amino acid deletion in the PRPF31 protein. In addition, the mRNA expression assay revealed that the mRNA expression level of the PRPF31 and RP9 genes were significantly lower in RP patients than controls (p < 0.05). Finally, the in vitro transfection assay demonstrated that the mRNA expression level of the mutant transfection group was significantly lower than the wild-type transfection group (p < 0.05). CONCLUSIONS: Our study suggested that the c.544_618del75bp mutation in the PRPF31 gene was a causative mutation in this ADRP family and affected the expression of RP9 gene by influencing the formation of U4/U6-U5 tri-snRNP, eventually leading to the occurrence of RP.

Quantitative analysis of lead sources in wheat tissue and grain under different lead atmospheric deposition areas.

Due to rapid growth of industrialization and human activities, such as mining and smelting, lead (Pb) has become a major environmental contaminant. As Pb can pose risks to human health, preventing Pb pollution in wheat is important for food safety, requiring accurate verification of pollution sources. Pb concentrations and isotope ratio levels in soil, in the atmosphere, and wheat tissue (root, stem, leaf, grain) in an area of high-Pb deposition (in the vicinity of a Pb smelter in Jiyuan city) and an area of low deposition (the northwest suburb of Zhengzhou city) were examined. The Pb isotope ratio and the binary mixed model were used to quantify the contribution of soil and atmospheric deposition to Pb content in wheat tissues. Results show that Pb content in soil, atmospheric deposition, and wheat in the high deposition area were significantly higher than those in the low deposition area. Pb content in soil, atmospheric deposition, wheat roots, stems, leaves, and grains in the high-deposition area were 355.32 ± 14.78, 5477.90 ± 187.85, 158.72 ± 9.56, 21.36 ± 1.72, 26.49 ± 1.96, and 0.94 ± 0.02 mg kg-1, respectively. Pb content in the low-deposition area were 6.10 ± 0.75, 78.50 ± 4.35, 2.47 ± 0.23, 1.03 ± 0.07, 2.11 ± 0.13, and 0.08 ± 0.01 mg kg-1, respectively. The Pb isotope ratio recorded obvious differences between soil and atmospheric deposition in the two areas. Combined with the significant correlation between Pb isotopes in various tissues of wheat and environmental media, and analysis of the isotopic composition characteristics of wheat and environmental media, in the high-deposition area, the contribution rate of atmospheric deposition in wheat roots, stems, leaves, and grains was 14%, 66%, 84%, and 77%, respectively. And the soil contribution rate was 86%, 34%, 16%, 23%, respectively. In the low-deposition area, the contribution rate of atmospheric deposition in wheat roots, stems, leaves, and grains was 49%, 73%, 93%, and 83%, respectively. And the soil contribution rates were 51%, 27%, 7%, and 17%, respectively. In the low-Pb deposition area, the contribution rate of atmospheric deposition in wheat was higher than that in the high-deposition area. Atmospheric deposition was the main source of Pb in grains, leaves, and stems of wheat in different depositional areas. Pb in wheat roots mainly derives from soil, and the Pb contribution rate of soil to wheat roots in the high-deposition area was significantly higher than that in the low-deposition area.

Direct evidence of lead contamination in wheat tissues from atmospheric deposition based on atmospheric deposition exposure contrast tests.

A field experiment was conducted to assess the atmospheric deposition effects on lead (Pb) contamination in wheat by two contrasting treatments: wheat exposed or not to atmospheric deposition. Plants were housed in a shed during wheat greening for the non-exposed treatment. The Pb contents of wheat during different growth stages, of soil and of atmospheric deposits were analysed and combined with Pb stable isotope data to quantify the contribution of atmospheric deposition and soil to Pb in wheat tissue. The Pb content in atmospheric deposits was significantly higher than those in soil and wheat tissue, and the Pb content in wheat tissue exposed to atmospheric deposition was significantly higher than the Pb content in non-exposed tissue (p < 0.05). The 206Pb/207Pb of soil was significantly higher than the 206Pb/207Pb of atmospheric deposits (p < 0.05), and soil and atmospheric deposition were the two sources of Pb in wheat tissue. Atmospheric deposition was the main source of wheat tissue Pb in the exposed treatment, and most of the wheat tissue Pb, except for that in the stem, also came from atmospheric deposition in the maturing stage. The proportion of Pb from atmospheric deposition in roots, stems and leaves evidently decreased after the shed was erected, and the contribution of Pb from atmospheric deposition to wheat tissue was significantly higher in the exposed treatment than in the non-exposed treatment (p < 0.05). This contrast test directly confirmed that atmospheric deposition was the main source of Pb in the wheat tissues. Therefore, taking measures to reduce the absorption of Pb by wheat from atmospheric deposition can effectively ensure food safety.