Dynamic 3D Fresnel incoherent correlation holography imaging based on single-shot mirrored phase-shifting technology.

Fresnel incoherent correlation holography (FINCH) records coaxial holograms for wide-field 3D imaging with incoherent light, but its temporal phase-shifting strategy makes dynamic imaging challenging. Here, we present a compact, portable single-shot mirrored phase-shifting (SSPMS) module that can be easily integrated into the FINCH system, achieving secondary modulation of self-interference beams to enable the simultaneous acquisition of four phase-shift holograms in a single exposure. Compared with previously reported methods that use diffraction gratings to spatially separate self-interference beams at specific angles, this module duplicates a laterally shifted mirrored beam using a simply modified Michelson interferometer, so the phase-shifting holograms obtained via this module are free from optical aberrations or higher-order diffracted light noises. The feasibility of the proposed method is experimentally demonstrated through imaging dynamic 3D grayscale scenes.

Generation of short microwave pulse trains based on a hybrid mode-locked optoelectronic oscillator.

A novel approach, to the best of our knowledge, for generating short microwave pulse trains based on a hybrid mode-locked optoelectronic oscillator (HML-OEO) is proposed and demonstrated. In the proposed scheme, a saturable absorber (SA) device is inserted into the active mode-locked OEO (AML-OEO) to compress the pulse width of the microwave pulse trains. Numerical simulations and experimental results show that the HML-OEO generates a short microwave pulse train with a repetition rate of 98.994 kHz through fundamental frequency mode locking, and its pulse width is compressed by about 50% compared to the AML-OEO. Additionally, in the experiment, microwave pulse trains with different repetition rates are generated by second-, third-, fourth-, and fifth-order harmonic mode locking, respectively. Compared to the AML-OEO, the HML-OEO achieves pulse compression effects of 49.3%, 49.8%, 49.4%, and 49.9%, respectively. Notably, compared to the AML-OEO, the proposed scheme also exhibits outstanding performance in frequency stability.

Signal-On Detection of Dopamine and Tyrosinase Using Tris(hydroxypropyl)phosphine as a New Lucigenin Chemiluminescence Coreactant.

Dopamine (DA) is a very imperative neurotransmitter in our body, since it contributes to several physiological processes in our body, for example, memory, feeling, cognition, cardiovascular diseases, and hormone secretion. Meanwhile, tyrosinase is a critical biomarker for several dangerous skin diseases, including vitiligo and melanoma cancer. Most of the reported chemiluminescent (CL) methods for monitoring DA and tyrosinase are signal-off biosensors. Herein, we introduce a new chemiluminescent "signal-on" system, lucigenin-tris(hydroxypropyl)phosphine (THPP), for the selective determination of DA and tyrosinase. THPP is well known as a versatile and highly water-soluble sulfhydryl-reducing compound that is more highly stable against air oxidation than common disulfide reductants. By employing THPP for the first time as an efficient lucigenin coreactant, the lucigenin-THPP system has shown a high CL response (approximately 16-fold) compared to the lucigenin-H2O2 classical CL system. Surprisingly, DA can remarkably boost the CL intensity of the lucigenin-THPP CL system. Additionally, tyrosinase can efficiently catalyze the conversion of tyramine to DA. Therefore, lucigenin-THPP was employed as an ultrasensitive and selective signal-on CL system for the quantification of DA, tyrosinase, and THPP. The linear ranges for the quantification of DA, tyrosinase, and THPP were 50-1000 nM, 0.2-50 µg/mL, and 0.1-800 µM, respectively. LODs for DA and tyrosinase were estimated to be 24 nM and 0.18 µg/mL, respectively. Additionally, the CL system has been successfully employed for the detection of tyrosinase in human serum samples and the assay of DA in human serum samples as well as in dopamine injection ampules with excellent obtained recoveries.

Quantitative proteomic analysis reveals hub proteins for high temperature-induced male sterility in bread wheat (Triticum aestivum L.).

High-temperature (HT) stress can induce male sterility in wheat; however, the underlying mechanisms remain poorly understood. This study examined proteomic alterations across three developmental stages between normal and HT-induced male-sterile (HT-ms) anthers in wheat. Utilizing tandem mass tags-based proteomics, we identified 2532 differentially abundant proteins (DAPs): 27 in the tetrad stage, 157 in the binuclear stage, and 2348 in the trinuclear stage. Analyses through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways indicated significant enrichment of these DAPs in seven pathways, namely phenylpropanoid biosynthesis, flavonoid biosynthesis, sphingolipid metabolism, MAPK signaling pathway, starch and sucrose metabolism, response to heat, and response to reactive oxygen species (ROS). Our results indicated the downregulation of DAPs associated with phenylpropanoid biosynthesis and starch and sucrose metabolism, which aligns with anther indehiscence and the lack of starch in HT-ms anthers. By contrast, DAPs in the ROS pathway were upregulated, which aligns with excessive ROS accumulation in HT-ms anthers. Additionally, we conducted protein-protein interaction analysis for the DAPs of these pathways, identifying 15 hub DAPs. The abundance of these hub proteins was confirmed through qRT-PCR, assessing mRNA expression levels of the corresponding transcripts. Collectively, these results offer insights into the molecular mechanisms underlying HT-induced male sterility in wheat at the proteomic level, providing a valuable resource for further research in plant sexual reproduction.

Self-Powered Electrochemiluminescence for Imaging the Corrosion of Protective Coating of Metal and Quantitative Analysis.

In almost all electrochemical systems for electrochemiluminescence (ECL) analysis, electrodes are connected with an external power source, either directly or via wireless energy transfer circuit. That is inconvenient and makes some applications impossible. Herein, we use galvanized iron with two different metals as both power source and electrodes to achieve a self-powered ECL and exploit ECL for the imaging of the corrosion of protective coating of widely used metal (e.g. galvanized iron) for the first time. The self-powered ECL enables the visualization of the deterioration of galvanic coating on iron using a smartphone and the detection of ascorbic acid with a linear range of 0.5-100 µM and a limit of detection of 0.31 µM. The devices based on self-powered approach do not require external power supply, thus effectively reducing their volume and cost. The self-powered ECL holds great promise for metal corrosion imaging and analytical applications.

VS4 Nanodendrites with Narrow Bandgaps in Activating Dissolved Oxygen for Boosted Chemiluminescence and Hemin Detection by Unexpected Quenching.

Chemiluminescence (CL)-based analytical methods utilize luminophores that need to be activated with an oxidizing agent to trigger CL emission. Despite its susceptibility to decomposition when exposed to external light or trace metals, hydrogen peroxide (H2O2) has been widely used to develop chemiluminescent methods due to the limited number of suitable alternatives for activating chemiluminescent luminophores. Also, analytical methods based on the well-known luminol/H2O2 CL system have low sensitivity. Dissolved oxygen (DO) is a naturally abundant and environmentally benign alternative oxidant for luminol and other CL luminophores. However, DO alone is inactive and needs an efficient catalyst or a coreaction accelerator for its activation. Because of the narrow bandgap of VS4 (ca. 1.12 eV), it can facilitate fast electron-transfer kinetics with an acceptor molecule such as DO. Here, we introduce vanadium tetrasulfide (VS4) to boost CL for the first time. Under the optimized conditions, VS4 nanodendrite catalyzes the generation of reactive oxygen species by activating DO which subsequently reacts with luminol to generate intense CL. It enhances the CL intensity of luminol/DO by about 10,000 times. Surprisingly, hemin remarkably quenches the generated CL of luminol/DO/VS4 nanodendrites, which is completely opposite to its typical enhancement of luminol CL. Based on the remarkable concentration-dependent quenching of the luminol/DO/VS4 nanodendrite CL by hemin, we have developed a sensitive CL method that can selectively detect hemin in the linear concentration range of 1-250 nM and achieved a limit of detection of 0.11 nM. The practical utility of the developed method was demonstrated by the determination of hemin in a pharmaceutical drug for the treatment of acute intermittent porphyria and in human serum. This study demonstrates that VS4 holds great promise in analytical method development.

Highly Sensitive Diethylamine Detection at Room Temperature Using g-C3N4 Nanosheets Decorated with CuO Hollow Polyhedral Structures.

Chemiresistive-based metal oxide semiconductor (MOS) gas sensors are widely used in gas sensing due to their advantageous properties. Graphitic carbon nitride (g-C3N4) and metal oxide heterostructure materials can improve charge transport properties, selectivity, and sensitivity in MOS gas sensor materials. Herein, for the first time, CuO hollow polyhedral structures (HPSs) were synthesized via a hydrothermal technique and annealed at different temperatures, with the 400 °C annealed (CuO-400 HPSs) demonstrating remarkable sensing capabilities for diethylamine (DEA) gas at room temperature (RT). The x-g-C3N4 nanosheets were decorated with CuO HPSs in varying amounts (x = 0.8, 1.8, 2.1, and 3.1 wt %) and then annealed at 400 °C for x-g-C3N4-CuO-400 hollow polyhedral heterostructures (HPHSs). Indeed, among the synthesized samples, the 1.8%-g-C3N4-CuO-400 HPHSs have a higher sensitivity to DEA (resistance change in gas (Rg) and air (Ra); Rg/Ra= 65 @ 20 ppm), a low detection limit (Rg/Ra= 6 @ 500 ppb), wide dynamic response (Rg/Ra= 190 @ 80 ppm), strong stability (30 days), and 21.6 times higher sensitivity than pure CuO at RT toward 20 ppm of DEA. The exceptional gas-sensing behavior can be attributed to various factors, including controlled annealing conditions that result in the formation of well-defined structures and greater porosity, efficient charge transfer properties resulting from an optimized ratio of g-C3N4 to CuO in HPHSs, an abundance of defects, unsaturated Cu sites, and synergistic effects. The study presents a universal strategy for generating sensitive and selective g-C3N4-based composite materials for low-temperature gas sensors.

Identification and characterization of the CRK gene family in the wheat genome and analysis of their expression profile in response to high temperature-induced male sterility.

Cysteine-rich receptor-like kinases (CRKs) play many important roles during plant development, including defense responses under both biotic and abiotic stress, reactive oxygen species (ROS) homeostasis, callose deposition and programmed cell death (PCD). However, there are few studies on the involvement of the CRK family in male sterility due to heat stress in wheat (Triticum aestivum L.). In this study, a genome-wide characterization of the CRK family was performed to investigate the structural and functional attributes of the wheat CRKs in anther sterility caused by heat stress. A total of 95 CRK genes were unevenly distributed on 18 chromosomes, with the most genes distributed on chromosome 2B. Paralogous homologous genes with Ka/Ks ratios less than 1 may have undergone strong purifying selection during evolution and are more functionally conserved. The collinearity analysis results of CRK genes showed that wheat and Arabidopsis (A. thaliana), foxtail millet, Brachypodium distachyon (B. distachyon), and rice have three, 12, 15, and 11 pairs of orthologous genes, respectively. In addition, the results of the network interactions of genes and miRNAs showed that five miRNAs were in the hub of the interactions map, namely tae-miR9657b-5p, tae-miR9780, tae-miR9676-5p, tae-miR164, and tae-miR531. Furthermore, qRT-PCR validation of the six TaCRK genes showed that they play key roles in the development of the mononuclear stage anthers, as all six genes were expressed at highly significant levels in heat-stressed male sterile mononuclear stage anthers compared to normal anthers. We hypothesized that the TaCRK gene is significant in the process of high-temperature-induced sterility in wheat based on the combination of anther phenotypes, paraffin sections, and qRT-PCR data. These results improve our understanding of their relationship.

Fresnel incoherent compressive holography toward 3D videography via dual-channel simultaneous phase-shifting interferometry.

Fresnel incoherent correlation holography (FINCH) enables high-resolution 3D imaging of objects from several 2D holograms under incoherent light and has many attractive applications in motionless 3D fluorescence imaging. However, FINCH has difficulty implementing 3D imaging of dynamic scenes since multiple phase-shifting holograms need to be recorded for removing the bias term and twin image in the reconstructed scene, which requires the object to remain static during this progress. Here, we propose a dual-channel Fresnel noncoherent compressive holography method. First, a pair of holograms with π phase shifts obtained in a single shot are used for removing the bias term noise. Then, a physic-driven compressive sensing (CS) algorithm is used to achieve twin-image-free reconstruction. In addition, we analyze the reconstruction effect and suitability of the CS algorithm and two-step phase-shift filtering algorithm for objects with different complexities. The experimental results show that the proposed method can record hologram videos of 3D dynamic objects and scenes without sacrificing the imaging field of view or resolution. Moreover, the system refocuses images at arbitrary depth positions via computation, hence providing a new method for fast high-throughput incoherent 3D imaging.

Singular multi-wavelength and multi-waveband transparencies generated by P T-symmetric dumbbell optical waveguide networks.

In this paper, we investigate the singular multi-wavelength and multi-waveband transparencies generated by P T-symmetric dumbbell optical waveguide networks composed of two materials, and obtain the number regularity for the transparency wavelengths of one-unit-cell system and the general relationships for the transmission and reflection coefficients of multi-unit-cell systems. Consequently, three types of exact transparencies produced by multi-unit-cell systems are found based on the aforementioned formulas: (i)exact multi-wavelength unidirectional or bidirectional transparency as the same as those of one-unit-cell system; (ii)exact multi-wavelength bidirectional transparency at which one-unit-cell system cannot produce exact transparency, generated by adjusting the number of unit cells; (iii)exact multi-wavelength bidirectional transparency at which one-unit-cell system produces exact transparency, also generated by adjusting the number of unit cells. It provides theoretical foundations for developing highly sensitive and multi-wavelength optical filters. On the other hand, we also discover that multi-unit-cell systems can create approximate multi-waveband bidirectional transparencies by adjusting the number of unit cells, which provides scientific support for developing high-performance optical stealth devices.

Much Stronger Chemiluminescence of 9-Mesityl-10-methylacridinium Ion than Lucigenin at Neutral Conditions for Co2+ Detection.

9-Mesityl-10-methylacridinium ion (Acr+-Mes) is a donor-acceptor molecule with a much longer lifetime and a higher energy electron transfer excited state than natural photosynthetic reaction centers. Unlike lucigenin with a coplanar geometry, Acr+-Mes has an orthogonal geometry. There is no π conjugation between Acr+ and Mes. Due to its special electron donor-acceptor structure, it does not rely on strong alkalinity to generate an electron transfer state like lucigenin, which makes it possible to achieve chemiluminescence (CL) under weakly alkaline or neutral conditions. In this study, we report Acr+-Mes CL for the first time. Acr+-Mes generates about 400 times stronger CL intensity than lucigenin under neutral conditions (pH = 7) using KHSO5 as the coreactant. Moreover, Co2+ can enhance Acr+-Mes/KHSO5 CL remarkably. Acr+-Mes/KHSO5 CL enables Co2+ detection with a linear range of 0.5-500 nM and a limit of detection of 28 pM (S/N = 3). This method was tested for the detection of Co2+ in lake water, and the standard recovery rate of 96.8-107% was achieved. This study provides a new way to develop efficient CL systems in neutral solutions.

Corrigendum: Genome-wide identification of genes encoding cystathionine beta synthase domain-containing proteins in wheat and its relationship with anther male sterility under heat stress.

[This corrects the article DOI: 10.3389/fpls.2022.1061472.].

Remarkably Enhanced Luminol/H2O2 Chemiluminescence with Excellent Peroxidase-like Activity of FeCoNi-based Metal-Organic Xerogels for the Sensitive Detection of Dopamine.

Metal-organic gels (MOGs) are a category of metal-organic smart soft materials with large specific surface areas, loose porous structures, and open metal active sites. In this work, trimetallic Fe(III)Co(II)Ni(II)-based MOGs (FeCoNi-MOGs) were synthesized at room temperature via a simple and mild one-step procedure. Fe3+, Co2+, and Ni2+ were the three central metal ions in it, while 1,3,5-benzenetricarboxylic acid (H3BTC) served as the ligand. The solvent enclosed in it was then removed by freeze-drying to get the corresponding metal-organic xerogels (MOXs). The as-prepared FeCoNi-MOXs have excellent peroxidase-like activity and can significantly enhance luminol/H2O2 chemiluminescence (CL) by more than 3000 times, which is very effective compared with other reported MOXs. Based on the inhibitory effect of dopamine on the CL of the FeCoNi-MOXs/luminol/H2O2 system, a simple, rapid, sensitive, and selective CL method for dopamine detection was established with a linear range of 5-1000 nM and a limit of detection of 2.9 nM (LOD, S/N = 3). Furthermore, it has been effectively used for the quantitative measurement of dopamine in dopamine injections and human serum samples, with a recovery rate of 99.5-109.1%. This research brings up prospects for the application of MOXs with peroxidase-like activity in CL.

Three-Channel Near-Field Display and Encryption Based on a Polarization Multiplexed Metasurface.

Multichannel metasurfaces are becoming a significant trend in the field of optical encryption due to their excellent manipulation of optical wavefronts. However, existent multichannel metasurfaces for optical encryption mostly implement only two channels in the near-field, or three channels by combining the near- and far-field. In this paper, we propose and simulate a three-channel metasurface that works entirely in the near-field and uses the polarization state of the incident light, left circularly polarized (LCP) light, right circularly polarized (RCP) light, and linearly polarized (LP) light as the security key. The metasurface consists of two types of nanostructures that work as a polarizer and a quarter-wave plate, providing an additional degree of freedom for encoding that enables independent near-field display at 633 nm wavelength incident light. The proposed three-channel metasurface has the advantages of high information density and high security, which will pave the way for multi-channel applications such as ultracompact displays, optical encryption, and information storage.

Generation and measurement of irregular polygonal perfect vortex optical beam based on all-dielectric geometric metasurface.

The perfect optical vortex (POV) beam carrying orbital angular momentum with topological charge-independent radial intensity distribution possesses ubiquitous applications in optical communication, particle manipulation, and quantum optics. But the mode distribution of conventional POV beam is relatively single, limiting the modulation of the particles. Here, we originally introduce the high-order cross-phase (HOCP) and ellipticity γ into the POV beam and construct all-dielectric geometric metasurfaces to generate irregular polygonal perfect optical vortex (IPPOV) beams following the trend of miniaturization and integration of optical systems. By controlling the order of the HOCP, conversion rate u, and ellipticity factor γ, various shapes of IPPOV beams with different electric field intensity distributions can be realized. In addition, we analyze the propagation characteristics of IPPOV beams in free-space, and the number and rotation direction of bright spots at the focal plane give the magnitude and sign of the topological charge carried by the beam. The method does not require cumbersome devices or complex calculation process, and provides a simple and effective method for simultaneous polygon shaping and topological charge measurement. This work further improves the beam manipulation ability while maintaining the characteristics of the POV beam, enriches the mode distribution of the POV beam, and provides more possibilities for particle manipulation.

Numerical Simulation of Integrated Generation and Shaping of Airy and Bessel Vortex Beams Based on All-Dielectric Metasurface.

Integrating multiple independent functions into a single optical component is one of the most important topics in research on photoelectric systems. In this paper, we propose a multifunctional all-dielectric metasurface that can achieve a variety of non-diffractive beams depending on the polarization state of the incident light. Using the anisotropic TiO2 rectangular column as the unit structure, the three functions of generating polygonal Bessel vortex beams under left-handed circularly polarized incidence, Airy vortex beams under right-handed circularly polarized incidence and polygonal Airy vortex-like beams under linearly polarized incidence are realized. In addition, the number of polygonal beam sides and the position of focal plane can be adjusted. The device could facilitate further developments in scaling complex integrated optical systems and fabricating efficient multifunctional components.

Strong enhancement of Goos-Hänchen shift through the resonant optical tunneling effect.

The resonant optical tunneling effect (ROTE) originates from the frustrated total reflection effect because unique transmission characteristics are used to study high-sensitivity sensors. In this study, we theoretically demonstrated that choosing a suitable transmission gap made it possible for the ROTE structure based on hexagonal boron nitride and graphene to obtain a large Goos-Hänchen shift as high as tens of thousands of times the incident wavelength at a specific incident angle. The amplitude of the Goos-Hänchen shift was found to be sensitive to the central layer thickness but was also modulated by the tunneling gap on both sides. In addition, adjusting the chemical potential and relaxation time of the graphene sheets could alter the Goos-Hänchen shift. Our work provides a new way to explore the Goos-Hänchen effect and opens the possibility for the application of high-precision measurement technology based on the ROTE.

Band-tunable achromatic metalens based on phase change material.

Achromatic metalens have the potential to significantly reduce the size and complexity of broadband imaging systems. A large variety of achromatic metalens has been proposed and most of them have the fixed achromatic band that cannot be actively modified. However, band-tunable is an important function in practical applications such as fluorescence microscopic imaging and optical detection. Here, we propose a bilayer metalens that can switch achromatic bands by taking the advantage of the high refractive index contrast of Sb2S3 between amorphous and crystalline state. By switching the state of Sb2S3, the achromatic band can be reversibly switched between the red region of visible spectrum (650-830 nm) and the near-infrared spectrum (830-1100 nm). This band-tunable design indicates a novel (to our knowledge) method to solve the problem of achromatic focusing in an ultrabroad band. The metalens have an average focusing efficiency of over 35% and 55% in two bands while maintaining diffraction-limited performance. Moreover, through proper design, we can combine different functionalities in two bands such as combining achromatic focusing and diffractive focusing. The proposed metalens have numerous potential applications in tunable displaying, detecting devices and multifunctional devices.

Dual-channel metasurfaces for independent and simultaneous display in near-field and far-field.

The operation of near-field and far-field can be employed to display holographic and nanoprinting images, which significantly improves the information density. Previous studies have proposed some approaches to display the images independently or simultaneously, but cannot satisfy these two characteristics in a single structure under the same incident light. Here, a single layer multifunctional metasurface is proposed to display a nanoprinting image and a holographic image independently and simultaneously. By tailoring the dimensions of each nanobricks and adopting different orientation angle, the amplitude and phase can be artificially designed. Moreover, enabled by the simulated annealing algorithm, we take the impact of both amplitude and phase of each nanobrick into consideration, which eliminates the unnecessary influence of amplitude on holographic image. Compared with previous work, our metasurfaces markedly improve the quality of holographic image with simple structures while not affecting the nanoprinting image. To be exact, it breaks the coupling between the near-field and far-field, achieving independent and simultaneous control of both fields. Our proposed metasurfaces carry characteristics of simple manufacture, little crosstalk, and great compactness, which provides novel applications for image displays, optical storage and information technology.

Extraordinary Characteristics of One-Dimensional PT-Symmetric Ring Optical Waveguide Networks Composed of Adjustable Length Ratio Waveguides.

A novel one-dimensional parity-time-symmetric periodic ring optical waveguide network (1D PTSPROWN) is constructed using magnesium fluoride (MgF2), by adjusting the length ratio of gain and loss materials in PT-symmetric waveguide and ordinary dielectric material, and by optimizing the program to search for the extremum spontaneous PT-symmetric breaking points. The ultra-strong transmission, reflection, and photonic location are noticed in the proposed 1DPTSPROWN as compared with the other PT-symmetric optical waveguide networks. The maximum and minimum reached 1018 and 10-15, respectively, which is more than 6 orders of magnitude greater and 3 orders of magnitude smaller than the best results reported so far. The ultra-strong transmission and reflection peaks, ultra-weak transmission, and reflection valleys generated by electromagnetic waves in this network were found to have interesting resonance and anti-resonance effects. Furthermore, frequency of periodic cycles and violet or redshift laws were discovered in the 1D PTSPROWN of fixed length ratio of gain and loss material in the PT-symmetric waveguide by adjusting the ratio of the upper and lower arm lengths of waveguides. The proposed optical waveguide network might have potential application in the design of CPA lasers, high-efficiency optical accumulators, and several other devices.