Dietary Chitosan Attenuates High-Fat Diet-Induced Oxidative Stress, Apoptosis, and Inflammation in Nile Tilapia (Oreochromis niloticus) through Regulation of Nrf2/Kaep1 and Bcl-2/Bax Pathways.

Fatty liver injury is a prevalent condition in most farmed fish, yet the molecular mechanisms underpinning this pathology remain largely elusive. A comprehensive feeding trial spanning eight weeks was conducted to discern the potential of dietary chitosan in mitigating the deleterious effects of a high-fat diet (HFD) while concurrently exploring the underlying mechanism. Growth performance, haemato-biochemical capacity, antioxidant capacity, apoptotic/anti-apoptotic gene expression, inflammatory gene expression, and histopathological changes in the liver, kidney, and intestine were meticulously assessed in Nile tilapia. Six experimental diets were formulated with varying concentrations of chitosan. The first three groups were administered a diet comprising 6% fat with chitosan concentrations of 0%, 5%, and 10% and were designated as F6Ch0, F6Ch5, and F6Ch10, respectively. Conversely, the fourth, fifth, and sixth groups were fed a diet containing 12% fat with chitosan concentrations of 0%, 5%, and 10%, respectively, for 60 days and were termed F12Ch0, F12Ch5, and F12Ch10. The results showed that fish fed an HFD demonstrated enhanced growth rates and a significant accumulation of fat in the perivisceral tissue, accompanied by markedly elevated serum hepatic injury biomarkers and serum lipid levels, along with upregulation of pro-apoptotic and inflammatory markers. In stark contrast, the expression levels of nrf2, sod, gpx, and bcl-2 were notably decreased when compared with the control normal fat group. These observations were accompanied by marked diffuse hepatic steatosis, diffuse tubular damage, and shortened intestinal villi. Intriguingly, chitosan supplementation effectively mitigated the aforementioned findings and alleviated intestinal injury by upregulating the expression of tight junction-related genes. It could be concluded that dietary chitosan alleviates the adverse impacts of an HFD on the liver, kidney, and intestine by modulating the impaired antioxidant defense system, inflammation, and apoptosis through the variation in nrf2 and cox2 signaling pathways.

Unlocking the critical roles of N, P Co-Doping in MXene for Lithium-Oxygen Batteries: Elevated d-Band center and expanded interlayer spacing.

The design and fabrication of bifunctional catalysts with high electrocatalytic activity and stability are critical for developing highly reversible Li-O2 batteries (LOBs). Herein, the N, P co-doped MXene (NP-MXene) is prepared by one-step annealing method and evaluated as bifunctional catalyst for LOBs. The results suggest that the P doping plays a crucial role in increasing interlayer distance of MXene, thereby effectively providing more active sites, fast mass transfer, and ample space for the deposition/decomposition of Li2O2. Moreover, the N doping can significantly elevate the d-band center of Ti, thereby remarkably improving the adsorption of reaction intermediates and accelerating the deposition/decomposition of Li2O2 films. Consequently, the MXene-based LOBs deliver an ultrahigh specific capacity of 13,995 mAh/g at 500 mA g-1, a discharge/charge voltage gap of 0.89 V, and a cycle life up to 523 cycles with a limited capacity of 1000 mAh/g at 500 mA g-1. Impressively, the as-fabricated flexible LOBs with NP-MXene cathode display excellent cycling stability and ability to continuously power LEDs even after bending. Our findings pave the road of heteroatom doped MXenes as next-generation electrodes for high-performance energy storage and conversion systems.

Palladium(II) Modulation Enhances the Water Stability and Aqueous 99TcO4-/ReO4- Removal Performance of Metal-Organic Frameworks.

Improving the water stability of metal-organic frameworks (MOFs) is essential for their use in water pollution treatment and environmental remediation, though it remains technically challenging. Herein, we report a novel cationic MOF constructed with [Th6O4(OH)4(COO)12] units and [CoN4·Cl2] units possessing a ftw-type topology (denoted as 1-Th-Co). 1-Th-Co itself exhibited poor water stability but excellent stability following a palladium(II) modulation strategy. Experimental studies reveal that Co(II) ions in 1-Th-Co were replaced by Pd(II) ions through cation exchange in N,N-diethylformamide (yielding 1-Th-Pd). The planar PdN4 units in 1-Th-Pd were responsible for improving the water stability of the framework. As a result, 1-Th-Pd offered excellent stability, fast adsorption kinetics, and high removal ratios for 99TcO4- and ReO4- (as a 99TcO4- surrogate) in contaminated water. When used in packed columns, 1-Th-Pd can dynamically capture ReO4- from groundwater. This work provides a new avenue for improving the water stability of MOFs, offering new vistas for the decontamination of aqueous solutions containing 99TcO4- and ReO4-.

The positive impact of introducing public engagement as a self-directed learning strategy in undergraduate nervous system education.

Undergraduate medical education in China has shifted from educator-centered learning to self-directed learning (SDL) over the past few decades. Careful design of public engagement activities can enable SDL and empower medical students to pioneer public health and patient safety education. In this study, we aimed to innovate nervous system education by implementing a public engagement model that empowers students to learn about the nervous system by teaching the public. Our goal was to generate greater interest in the nervous system at the undergraduate stage, inspire students' enthusiasm to pursue a career in neurology, and ultimately, contribute to health promotion. During the nervous system module of the second year of the undergraduate curriculum, students were given the option to participate in the public engagement model. Participants were tasked with the creation of educational videos focusing on knowledge, attitudes, and behaviors associated with the prevention and management of neurological diseases and their complications. The videos were made accessible to the general public through the university's official channel at the end of the semester. A total of 117 students (67.24% of all students) chose to participate in the public engagement model. Female students and those with higher Grade Point Averages in the present semester were more likely to participate. The model received strong positive feedback from participants, as students found the public engagement task helpful in learning about the nervous system module as well as in enhancing their public engagement skills. Despite the time and effort consumption, participating in the public engagement task did not affect students' exam scores. The public engagement task is an innovative model in the nervous system curriculum and has the potential to be integrated into a broader range of undergraduate courses. It empowers medical students to pioneer public health and patient safety education.

The application of innovative ecosystems to build resilient communities in response to major public health events.

In recent years, major public health events have had a significant and far-reaching impact on communities. As a response, there has been an increasing interest in enhancing community resilience through innovative ecosystems that involve diverse stakeholders with varying needs and demands. This study investigates the application of innovative ecosystems to improve community resilience in the face of major public health events by utilizing a sequential game approach to balance the interests of government, community, and residents. Subsequently, a comprehensive questionnaire survey was conducted among key stakeholders to ascertain their objectives, requirements and concerns for the innovation ecosystem based on the analysis results of the game model. The reliability and effectiveness of the proposed research method were verified through the analysis and verification of the sequence game model and questionnaire survey results. Finally, according to our analysis results, we propose countermeasures for promoting innovative ecosystems to improve community resilience. The research results indicate that the successful implementation of innovative ecosystems requires consideration of the different needs of stakeholders such as government officials, community members, and residents. Combining these perspectives can effectively promote such systems while enhancing the community's resilience to major public health events.

Theoretical Study of the Ternary Compound Monolayer CuP2Se for Photocatalytic Water Splitting with Efficient Optical Absorption.

Utilizing photocatalytic method to produce hydrogen by splitting water is an efficient strategy to solve the hotspot issues of energy crisis and environmental pollution. Herein, we systematically investigate the corresponding properties of the reported Cu-bearing ternary compound monolayer CuP2Se by using the first-principle calculations. The monolayer CuP2Se has quite small cleavage energy of 0.51 J/m2, indicating it can be easily produced by the mechanical exfoliation method experimentally. In addition, it is an indirect bandgap semiconductor material which has a moderate value of 1.91 eV. The conduction band minimum (CBM) and valence band maximum (VBM) can perfectly straddle the redox potentials of water when a biaxial strain of -4% to 4% is applied, unveiling the high photocatalytic thermodynamic stability of monolayer CuP2Se in response to the effect of solvent tension. Remarkably, the monolayer CuP2Se also demonstrates significant sunlight capturing ability in the visible region. The outstanding electronic and optical properties suggest that the monolayer CuP2Se is undoubtedly a viable material for photocatalytic water splitting.

Terminally differentiated cytotoxic CD4+ T cells were clonally expanded in the brain lesion of radiation-induced brain injury.

BACKGROUND: Accumulating evidence supports the involvement of adaptive immunity in the development of radiation-induced brain injury (RIBI). Our previous work has emphasized the cytotoxic function of CD8+ T cells in RIBI. In this study, we aimed to investigate the presence and potential roles of cytotoxic CD4+ T cells (CD4+ CTLs) in RIBI to gain a more comprehensive understanding of adaptive immunity in this context. MAIN TEXT: Utilizing single-cell RNA sequencing (scRNA-seq), we analyzed 3934 CD4+ T cells from the brain lesions of four RIBI patients and identified six subclusters within this population. A notable subset, the cytotoxic CD4+ T cells (CD4+ CTLs), was marked with high expression of cytotoxicity-related genes (NKG7, GZMH, GNLY, FGFBP2, and GZMB) and several chemokine and chemokine receptors (CCL5, CX3CR1, and CCL4L2). Through in-depth pseudotime analysis, which simulates the development of CD4+ T cells, we observed that the CD4+ CTLs exhibited signatures of terminal differentiation. Their functions were enriched in protein serine/threonine kinase activity, GTPase regulator activity, phosphoprotein phosphatase activity, and cysteine-type endopeptidase activity involved in the apoptotic signaling pathway. Correspondingly, mice subjected to gamma knife irradiation on the brain showed a time-dependent infiltration of CD4+ T cells, an increase of MHCII+ cells, and the existence of CD4+ CTLs in lesions, along with an elevation of apoptotic-related proteins. Finally, and most crucially, single-cell T-cell receptor sequencing (scTCR-seq) analysis at the patient level determined a large clonal expansion of CD4+ CTLs in lesion tissues of RIBI. Transcriptional factor-encoding genes TBX21, RORB, and EOMES showed positive correlations with the cytotoxic functions of CD4+ T cells, suggesting their potential to distinguish RIBI-related CD4+ CTLs from other subsets. CONCLUSION: The present study enriches the understanding of the transcriptional landscape of adaptive immune cells in RIBI patients. It provides the first description of a clonally expanded CD4+ CTL subset in RIBI lesions, which may illuminate new mechanisms in the development of RIBI and offer potential biomarkers or therapeutic targets for the disease.

Fundamentally Manipulating the Electronic Structure of Polar Bifunctional Catalysts for Lithium-Sulfur Batteries: Heterojunction Design versus Doping Engineering.

Heterogeneous structures and doping strategies have been intensively used to manipulate the catalytic conversion of polysulfides to enhance reaction kinetics and suppress the shuttle effect in lithium-sulfur (Li-S) batteries. However, understanding how to select suitable strategies for engineering the electronic structure of polar catalysts is lacking. Here, a comparative investigation between heterogeneous structures and doping strategies is conducted to assess their impact on the modulation of the electronic structures and their effectiveness in catalyzing the conversion of polysulfides. These findings reveal that Co0.125Zn0.875Se, with metal-cation dopants, exhibits superior performance compared to CoSe2/ZnSe heterogeneous structures. The incorporation of low Co2+ dopants induces the subtle lattice strain in Co0.125Zn0.875Se, resulting in the increased exposure of active sites. As a result, Co0.125Zn0.875Se demonstrates enhanced electron accumulation on surface Se sites, improved charge carrier mobility, and optimized both p-band and d-band centers. The Li-S cells employing Co0.125Zn0.875Se catalyst demonstrate significantly improved capacity (1261.3 mAh g-1 at 0.5 C) and cycle stability (0.048% capacity delay rate within 1000 cycles at 2 C). This study provides valuable guidance for the modulation of the electronic structure of typical polar catalysts, serving as a design directive to tailor the catalytic activity of advanced Li-S catalysts.

Tanshinone IIA ameliorates energy metabolism dysfunction of pulmonary fibrosis using 13C metabolic flux analysis.

Evidence indicates that metabolic reprogramming characterized by the changes in cellular metabolic patterns contributes to the pathogenesis of pulmonary fibrosis (PF). It is considered as a promising therapeutic target anti-PF. The well-documented against PF properties of Tanshinone IIA (Tan IIA) have been primarily attributed to its antioxidant and anti-inflammatory potency. Emerging evidence suggests that Tan IIA may target energy metabolism pathways, including glycolysis and tricarboxylic acid (TCA) cycle. However, the detailed and advanced mechanisms underlying the anti-PF activities remain obscure. In this study, we applied [U-13C]-glucose metabolic flux analysis (MFA) to examine metabolism flux disruption and modulation nodes of Tan IIA in PF. We identified that Tan IIA inhibited the glycolysis and TCA flux, thereby suppressing the production of transforming growth factor-ß1 (TGF-ß1)-dependent extracellular matrix and the differentiation and proliferation of myofibroblasts in vitro. We further revealed that Tan IIA inhibited the expression of key metabolic enzyme hexokinase 2 (HK2) by inhibiting phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/hypoxia-inducible factor 1α (HIF-1α) pathway activities, which decreased the accumulation of abnormal metabolites. Notably, we demonstrated that Tan IIA inhibited ATP citrate lyase (ACLY) activity, which reduced the collagen synthesis pathway caused by cytosol citrate consumption. Further, these results were validated in a mouse model of bleomycin-induced PF. This study was novel in exploring the mechanism of the occurrence and development of Tan IIA in treating PF using 13C-MFA technology. It provided a novel understanding of the mechanism of Tan IIA against PF from the perspective of metabolic reprogramming.

Engineering the pore environment of antiparallel stacked covalent organic frameworks for capture of iodine pollutants.

Radioiodine capture from nuclear fuel waste and contaminated water sources is of enormous environmental importance, but remains technically challenging. Herein, we demonstrate robust covalent organic frameworks (COFs) with antiparallel stacked structures, excellent radiation resistance, and high binding affinities toward I2, CH3I, and I3- under various conditions. A neutral framework (ACOF-1) achieves a high affinity through the cooperative functions of pyridine-N and hydrazine groups from antiparallel stacking layers, resulting in a high capacity of ~2.16 g/g for I2 and ~0.74 g/g for CH3I at 25 °C under dynamic adsorption conditions. Subsequently, post-synthetic methylation of ACOF-1 converted pyridine-N sites to cationic pyridinium moieties, yielding a cationic framework (namely ACOF-1R) with enhanced capacity for triiodide ion capture from contaminated water. ACOF-1R can rapidly decontaminate iodine polluted groundwater to drinking levels with a high uptake capacity of ~4.46 g/g established through column breakthrough tests. The cooperative functions of specific binding moieties make ACOF-1 and ACOF-1R promising adsorbents for radioiodine pollutants treatment under practical conditions.

COF-based membranes for liquid phase separation: Preparation, mechanism and perspective.

Covalent organic frameworks (COFs) are a new kind of crystalline porous materials composed of organic molecules connected by covalent bonds, processes the characteristics of low density, large specific surface area, adjustable pore size and structure, and easy to functionalize, which have been widely used in the field of membrane separation technology. Recently, there are more and more researches focusing on the preparation methods, separation application, and mechanism of COF membranes, which need to be further summarized and compared. In this review, we primarily summarized several conventional preparation methods, such as two-phase interfacial polymerization, in-situ growth on substrate, unidirectional diffusion method, layer-by-layer assembly method, mixed matrix membranes, and so on. The advantages and disadvantages of each method are briefly summarized. The application potential of COF membrane in liquid separation are introduced from four aspects: dyeing wastewater treatment, heavy metal removal, seawater desalination and oil-water separation. Then, the mechanisms including pore structure, hydrophilic/hydrophobic, electrostatic repulsion/attraction and Donnan effect are introduced. For the efficient removal of different kind of pollutions, researchers can select different ligands to construct membranes with specific pore size, hydrophily, salt or organic rejection ability and functional group. The ideas for the design and preparation of COF membranes are introduced. Finally, the future direction and challenges of the next generation of COF membranes in the field of separation are prospected.

Preparation of sea buckthorn (Hippophae rhamnoides L.) seed meal peptide by mixed fermentation and its effect on volatile compounds and hypoglycemia.

This study employed mixed bacterial strains to ferment seabuckthorn seed meal into peptides, and conducted a comprehensive evaluation of the growth adaptive conditions, molecular weight distribution, volatile compounds, and in vitro hypoglycemic activity required for fermentation. Results showed that when the amount of maltose was 1.1% and MgSO4·7H2O was added at 0.15 g/L, the peptide yield reached 43.85% with a mixed fermentation of Lactobacillus fermentum, Bacillus subtilis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Components with a molecular weight below 1 kDa were found to be more effective in inhibiting the activity of α-amylase and α-glucosidase, with the identified sequence being FYLPKM. Finally, SPME/GC-MS results showed that 86 volatile components were detected during the fermentation of seabuckthorn seed meal, including 22 alcohols, 9 acids, 7 ketones, 14 alkanes, 20 esters, and 14 other compounds. With prolonged fermentation time, the content of acids and esters increased significantly.

Research on the construction of a "full-chain" rapid response system for power emergencies.

A crucial industry for improving society's sustainable development is the power sector. To address issues with the ineffectiveness of electric power emergency response during emergencies and the unclear division of duty among emergency subjects. A prefecture-level city power supply company to respond to the "In-Fa" typhoon, for example, to build a "1 + N" two-level emergency rapid response unit. Furthermore, it is proposed from the emergency response, emergency coordination, emergency material reserves, etc., to build a "full-chain" type of power emergency quick reaction system. Case studies have revealed that the quick response system's emergency combat capability, catastrophe preventive and mitigation capability, and emergency security capability have all improved. The construction of a "full-chain" type of power emergency rapid response system specialized and standardized the power emergency response system and provided a reference basis for the power industry's emergency response.

CM-GAN: A Cross-Modal Generative Adversarial Network for Imputing Completely Missing Data in Digital Industry.

Multimodal data fusion analysis is essential to model the uncertainty of environment awareness in digital industry. However, due to communication failure and cyberattack, the sampled time-series data often have the issue of data missing. In some extreme cases, part of units are unobservable for a long time, which results in complete data missing (CDM). To impute missing data, many models have been proposed. However, they cannot address the CDM issue, because no observation data of the unobservable units can be obtained in this case. Thus, to address the CDM issue, a novel cross-modal generative adversarial network (CM-GAN) is proposed in this article. It combines the cross-modal data fusion technique and the deep adversarial generation technique to construct a cross-modal data generator. This generator can generate long-term time-series data from widely existing spatio-temporal modal data in modern industrial system, and then impute missing value by replacing them with generated data. To test the performance of CM-GAN, extensive experiments are conducted on photovoltaic (PV) power output dataset. Compared with other baseline models, the performance of CM-GAN is generally better and reaches the state-of-the-art level. Moreover, sufficient ablation studies are conducted to present the contribution of the cross-modal data fusion technique and show the reasonability of parameter settings of CM-GAN. Apart from this, some prediction experiments are also conducted. The results show that the PV data recovered by CM-GAN can provide more predictability information for improving the prediction accuracy of deep learning model.

Visualizing intracellular membrane interactions and cell type-specific differentiation in ferroptosis and apoptosis with Boranil-Carbazole derivative.

Intracellular lipid systems play essential roles in various physiological functions and cell growth processes. However, our understanding of the intricate interactions within this system, especially between mitochondria and lipid droplets, is limited, particularly in the context of cancer cells' altered lipid metabolism. To address this, our study introduces an N-B-O BODIPY-hexylcarbazole derivative, named Cz-Boranil, that sets a new benchmark in visualizing these critical interactions. Cz-Boranil's unique capability lies in its ability to display distinct intracellular distribution patterns in both normal and cancer cells, offering nuanced cell type-specific differentiation. More impressively, this probe tracks the coordinated interactions of lipid droplets and mitochondria during the critical processes of ferroptosis and apoptosis. We believe that the innovative capabilities of Cz-Boranil will revolutionize our understanding of intracellular lipid interactions and prove pivotal in identifying and studying cancerous cells.

Pore Space Partition Synthetic Strategy in Imine-linked Multivariate Covalent Organic Frameworks.

Partitioning the pores of covalent organic frameworks (COFs) is an attractive strategy for introducing microporosity and achieving new functionality, but it is technically challenging to achieve. Herein, we report a simple strategy for partitioning the micropores/mesopores of multivariate COFs. Our approach relies on the predesign and synthesis of multicomponent COFs through imine condensation reactions with aldehyde groups anchored in the COF pores, followed by inserting additional symmetric building blocks (with C2 or C3 symmetries) as pore partition agents. This approach allowed tetragonal or hexagonal pores to be partitioned into two or three smaller micropores, respectively. The synthesized library of pore-partitioned COFs was then applied for the capture of iodine pollutants (i.e., I2 and CH3I). This rich inventory allowed deep exploration of the relationships between the COF adsorbent composition, pore architecture, and adsorption capacity for I2 and CH3I capture under wide-ranging conditions. Notably, one of our developed pore-partitioned COFs (COF 3-2P) exhibited greatly enhanced dynamic I2 and CH3I adsorption performances compared to its parent COF (COF 3) in breakthrough tests, setting a new benchmark for COF-based adsorbents. Results present an effective design strategy toward functional COFs with tunable pore environments, functions, and properties.

Degradation and potential metabolism pathway of polystyrene by bacteria from landfill site.

Microplastics pollution has garnered significant attention in recent years. The unique cross-linked structure of polystyrene microplastics makes them difficult to biodegrade. In this study, we investigated the microbial community in landfill soil that has the ability to degrade polystyrene, as well as two isolated strains, named Lysinibacillus sp. PS-L and Pseudomonas sp. PS-P. The maximum weight loss of polystyrene film and microplastic in 30 days is 2.25% and 6.99% respectively. The water contact angle of polystyrene film decreased by a maximum of 35.70% during biodegradation. The increase in hydrophilicity is attributed to the oxidation reaction and formation of hydroxyl groups during the degradation of polystyrene. The carbon and oxygen element contents of polystyrene decreased and increased by a maximum of 3.81% and 0.79% respectively. The peak intensity changes at wavelengths of 3285-3648 cm-1 and 1652 cm-1 in Fourier transform infrared spectroscopy confirmed the formation of hydroxyl and carbonyl groups. Furthermore, quantitative PCR revealed the gene expression levels of alkane monooxygenase and alcohol dehydrogenase were upregulated by 8.8-fold and 8.5-fold respectively in PS biodegradation. Additionally, genome annotation of Pseudomonas sp. PS-P identified nine genes associated with polystyrene metabolism. These findings highlight Pseudomonas sp. PS-P as a potential candidate strain for polystyrene degradation enzymes or genes. Thus, they lay the groundwork for understanding the potential metabolic mechanisms and pathways involved in polystyrene degradation.

Highly Selective Extraction of U(VI) from Solutions by Metal Organic Framework-Based Nanomaterials through Sorption, Photochemistry, and Electrochemistry Strategies.

With the rapid development of nuclear technology and peaceful utilization of nuclear energy, plentiful U(VI) not only is required to be extracted from solutions for a sustainable nuclear fuel supply but also is inevitably released into the surrounding environment to result in pollution and threaten human health. Thereby, realizing selective extraction of U(VI) from aqueous solutions is crucial for U(VI) pollution control and a sustainable nuclear industry. Metal organic frameworks (MOFs) have gained multidisciplinary attention due to their excellent properties including large specific surface areas, tunable pore structures, easy functionalization, etc. This Review comprehensively summarizes the research progress of MOFs and MOF-based materials on U(VI) removal from aqueous solutions by sorption, photocatalysis, electrocatalysis, membrane separation, etc. The efficient high extraction ability is dependent on the intrinsic properties of MOFs and the techniques used. The removal properties of MOF-based materials as adsorbents, photocatalysts, and electrocatalysts for U(VI) are discussed. Information about the interaction mechanisms between U(VI) and MOF-based materials are analyzed in-depth, including experiments, theoretical calculations, and advanced spectroscopy analysis. The removal properties for U(VI) of various MOF-based materials are assessed through different techniques. Finally, a summary and perspective on the direction and challenges of MOF-based materials and various pollutant removal technologies are proposed to provide some significant information on designing and fabricating MOF-based materials for environmental pollution management.

Recent progress of covalent organic frameworks membranes: Design, synthesis, and application in water treatment.

To date, significant efforts have been devoted to eliminating hazardous components to purify wastewater through the development of various nanomaterials. Covalent organic frameworks (COFs), an important branch of the porous crystalline family, possess the peculiarity of ultrahigh surface area, adjustable pore size, and facile functionality. Exciting studies from design fabrication to potential applications in water treatment by COF-based membranes (COMs) have emerged. This review summarizes various preparation strategies and synthesis mechanisms for COMs, including layer-by-layer stacking, in situ growth, interfacial polymerization, and electrochemical synthesis, and briefly describes the advanced characterization techniques for COMs. Moreover, the application of COMs in heavy metal removal, dye separation, purification of radionuclides, pollutant detection, sea water desalination, and so on, is described and discussed. Finally, the perspectives on future opportunities for designing COMs in water purification have been proposed.

Effects of adding milk to fermented black mulberry (Morus nigra L.) juice on its antioxidant activity in C2C12 cells and changes in volatile flavor compounds during storage.

This study assessed the impact of milk on the bioactive compounds, physicochemical properties, antioxidant activity, ROS inhibition, and volatile flavor compounds of fermented black mulberry juice (FBMJ). Firstly, the results showed that 25% concentration of milk was the most suitable for preparing FBMJ-Milk. Compared to the control group, the addition of milk significantly increased the SOD activity and antioxidant capacity, as well as enhanced the total phenolic content (TPC) and SOD storage stability. Secondly, HS-SPME-GC-MS combined with OPLS-DA analysis identified 49 compounds in FBMJM, including 12 esters, 6 acids, 1 ketone, 2 aldehydes, 19 alcohols and 9 other compounds. During the storage, the levels of ethyl ester compounds decreased significantly, while the degradation of ester produced some acid and alcohol compounds. The findings revealed that the addition of milk was beneficial for maintaining the antioxidant stability of FBMJM during storage and enhancing the richness of product flavor.