Assessing indicators of arsenic toxicity using variable fluorescence in a commercially valuable microalgae: Physiological and toxicological aspects.

Indicators signaling Arsenic (As) stress through physiology of microalgae using non-destructive methods like variable fluorescence are rare but requisite. This study reports stress markers indicating arsenic (As) toxicity (in two concentrations 11.25 µg/L and 22.5 µg/L compared to a control) exposed to a microalga (Diacronema lutheri), using fast repetition rate fluorometry (FRRf). Growth and physiological parameters such as cell density, chl a and the maximum quantum yield Fv/Fm showed coherence and impeded after the exponential phase (day 9 - day 12) in As treatments compared to the control (p < 0.05). On contrary photo-physiological constants were elevated showing higher optical (aLHII) and functional [Sigma (σPSII)] absorption cross-section for the As treatments (p < 0.05) further implying the lack of biomass production yet an increase in light absorption. In addition, As exposure increased the energy dissipation by heat (NPQ-NSV) showing a strong relationship with the de-epoxidation ratio (DR) involving photoprotective pigments. Total As bioaccumulation by D. lutheri showed a strong affinity with Fe adsorption throughout the algal growth curve. This study suggests some prompt photo-physiological proxies signaling As contamination and endorsing its usefulness in risk assessments, given the high toxicity and ubiquitous presence of As in the ecosystem.

Underwater light climate and wavelength dependence of microalgae photosynthetic parameters in a temperate sea.

Studying how natural phytoplankton adjust their photosynthetic properties to the quantity and quality of underwater light (i.e. light climate) is essential to understand primary production. A wavelength-dependent photoacclimation strategy was assessed using a multi-color pulse-amplitude-modulation chlorophyll fluorometer for phytoplankton samples collected in the spring at 19 locations across the English Channel. The functional absorption cross section of photosystem II, photosynthetic electron transport (PETλ) parameters and non-photochemical quenching were analyzed using an original approach with a sequence of three statistical analyses. Linear mixed-effects models using wavelength as a longitudinal variable were first applied to distinguish the fixed effect of the population from the random effect of individuals. Population and individual trends of wavelength-dependent PETλ parameters were consistent with photosynthesis and photoacclimation theories. The natural phytoplankton communities studied were in a photoprotective state for blue wavelengths (440 and 480 nm), but not for other wavelengths (green (540 nm), amber (590 nm) and light red (625 nm)). Population-detrended PETλ values were then used in multivariate analyses (partial triadic analysis and redundancy analysis) to study ecological implications of PETλ dynamics among water masses. Two wavelength ratios based on the microalgae saturation parameter Ek (in relative and absolute units), related to the hydrodynamic regime and underwater light climate, clearly confirmed the physiological state of microalgae. They also illustrate more accurately that natural phytoplankton communities can implement photoacclimation processes that are influenced by in situ light quality during the daylight cycle in temporarily and weakly stratified water. Ecological implications and consequences of PETλ are discussed in the context of turbulent coastal ecosystems.