Assuntos
Portador Sadio/transmissão , Infecção Hospitalar/microbiologia , Infecção Hospitalar/transmissão , Pessoal de Saúde , Resistência a Meticilina , Infecções Estafilocócicas/transmissão , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificação , Portador Sadio/microbiologia , Impressões Digitais de DNA , DNA Bacteriano/genética , Surtos de Doenças , Eletroforese em Gel de Campo Pulsado , Genótipo , Humanos , Recém-Nascido , Unidades de Terapia Intensiva Neonatal , Itália/epidemiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificaçãoRESUMO
A point-prevalence study of nosocomial infections was conducted in 10 general hospitals in northwestern Italy in June and July 2000. Infection rates were compared by type and site among the different hospitals. Urinary tract infections were most frequent, accounting for 57.8% of 128 nosocomial infections.
Assuntos
Infecção Hospitalar/epidemiologia , Infecção Hospitalar/prevenção & controle , Estudos Transversais , Humanos , Incidência , Itália/epidemiologia , Prevalência , Fatores de RiscoRESUMO
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
Assuntos
Hidrolases de Éster Carboxílico/metabolismo , Saccharomyces cerevisiae/enzimologia , Carboxilesterase , Eletroforese em Gel de Poliacrilamida/métodos , Fluoresceínas , Dodecilsulfato de Sódio , Especificidade por SubstratoRESUMO
The A.A. studied the effectiveness of colliriums containing fibronectins aprotinin, and fibronectin associated with aprotinin compared to a placebo using 12 rabbits in which a deep corneal ulcer was induced by alkal. The results showed the same effectiveness of the three collyrium versus the placebo there was clinical recovery in all cases and restoration of corneal trasparency for 13 out of 15 treated eyes. Cellular cultures obtained from primary explants of treated corneal cells, compared with non treated groups, gave the same outcome of the clinical's ones.