Role of the molecular structure of humified organic matter in rice plant response to environmental lead pollution.

Humified organic matter has been shown to decrease Pb toxicity in plants. However, there are still gaps in our understanding of the mechanism by which this phenomenon occurs. In this study, we aimed to assess the ability of humic substances (HSs), humic acids (HAs), and fulvic acids (FAs) to enhance defense mechanisms in rice plants under lead (Pb)-stressed conditions. HS fractions were isolated from vermicompost using the chemical fractionation methodology established by the International Humic Substances Society. These fractions were characterized by solid-state NMR and FTIR. Chemometric analysis was used to compare humic structures and correlate them with bioactivity. Three treatments were tested to evaluate the protective effect of humic fractions on rice plants. The first experiment involved the application of humic fractions along with Pb. The second comprised pretreatment with humic fractions followed by subsequent exposure to Pb stress. The third experiment involved Pb stress and subsequent treatment with humic fractions. The root morphology and components of the antioxidative defense system were evaluated and quantified. The results showed that HS + Pb, HA + Pb, and FA + Pb treatment preserved root growth and reduced the levels of O2- and malondialdehyde (MDA) in the roots by up to 5% and 2%, respectively. Pretreatment of the plants with humic fractions promoted the maintenance of root growth and reduced the contents of O2-, H2O2, and MDA by up to 48%, 22%, and 20%, respectively. Combined application of humic fractions and Pb reduced the Pb content in plant tissues by up to 60%, while pretreatment reduced it by up to 80%. The protective capacity of humic fractions is related to the presence of peptides, lignin, and carbohydrate fragments in their molecular structures. These results suggest that products could be developed that can mitigate the adverse effects of heavy metals on agricultural crops.

Green Synthesis of Er-Doped ZnO Nanoparticles: An Investigation on the Methylene Blue, Eosin, and Ibuprofen Removal by Photodegradation.

We present a study on the green synthesis of undoped and Er-doped ZnO compounds using Mangifera indica gum (MI). A set of tests were conducted to assess the structure of the material. The tests included X-ray diffraction, Raman, and Fourier-transform infrared spectroscopy. Optical properties were studied using diffuse reflectance and photoluminescence. Morphological and textural investigations were done using SEM images and N2 adsorption/desorption. Furthermore, photocatalytic tests were performed with methylene blue (MB), yellow eosin (EY), and the pharmaceutical drug ibuprofen (IBU) under UV irradiation. The study demonstrated that replacing the stabilizing agent with Mangifera indica gum is an effective method for obtaining ZnO nanoparticles. Additionally, the energy gap of the nanoparticles exhibits a slight reduction in value. Photoluminescence studies showed the presence of zinc vacancies and other defects in both samples. In the photocatalytic test, the sample containing Er3+ exhibited a degradation of 99.7% for methylene blue, 81.2% for yellow eosin, and 52.3% for ibuprofen over 120 min. In the presence of methyl alcohol, the degradation of MB and EY dyes is 16.7% and 55.7%, respectively. This suggests that hydroxyl radicals are responsible for the direct degradation of both dyes. In addition, after the second reuse, the degradation rate for MB was 94.08%, and for EY, it was 82.35%. For the third reuse, the degradation rate for MB was 97.15%, and for EY, it was 17%. These results indicate the significant potential of the new semiconductor in environmental remediation applications from an ecological synthesis.

Gut Microbiota Modulation by Bioactive Compounds from Ilex paraguariensis: an In Vivo Study.

Yerba-mate (Ilex paraguariensis) is recognized for its biocompounds and bioactive properties. This study aimed to assess the potential of yerba-mate extract to modulate the intestinal microbiota in rats. After the ethical committee approval (CEUA - UPF, number 025/2018), the Wistar rats were given a daily dose of 3.29 mg of phenolic compounds per animal for 45 days. The antioxidant activity of the extract was assessed by ABTS and FRAP assays and the total phenolic compounds was measured at different pH levels. Identification and quantification of chlorogenic acid isomers were carried out using high-performance liquid chromatography (HPLC). Intestinal microbiota modulation was evaluated by administering the yerba-mate extract or water (control) to Wistar rats via intragastric gavage and its efficiency was measured through PCR. The antioxidant capacity of the yerba-mate extract was 64.53 ± 0.26 µmol Trolox/mL (ABTS) and 52.96 ± 0.86 µmol Trolox/mL (FRAP). The total phenolic compounds showed higher levels at pH 7.5 compared to pH 2.0. Chlorogenic acid isomers were found in greater abundance, with a concentration of 14.22 g/100 g. The administration of the extract resulted in positive modulation of the intestinal microbiota, specifically for the genera Lactobacillus sp. and Prevotella sp. The increase of these genera is related to the promotion of homeostasis of the gut microbiota. Therefore, these findings indicate that yerba-mate extract possesses significant antioxidant activity and can effectively modulate the intestinal microbiota in rats. These results support the potential use of yerba-mate as an alternative for controlling and preventing diseases associated with intestinal dysbiosis.

Endoscopic treatment of gastroesophageal reflux: a narrative review.

Gastroesophageal reflux disease (GERD) is a common chronic disease that affects one-third of the population worldwide. In recent years, there have been significant advances for diagnostic workup, which leads to better identification of reflux-related complications. Classically, the mainstay of therapy has been proton pump inhibitor and lifestyle and dietary modifications. For refractory GERD the gold-standard therapies are surgical antireflux procedures. Recently, endoscopic procedures have emerged as safe and efficient alternatives to surgery. These could represent a less invasive approach, with scarce morbidity and with a well-tolerated profile. Each of the existing endoscopic techniques for the treatment of GERD are addressed in this report, highlighting their potential advantages, aiming at helping decide the best management of these patients. Future studies, with larger numbers of patients, may allow a definitive role for these techniques in the management of GERD to be established.

Do patients undergoing physical therapy in a rehabilitation center have a high prevalence of pelvic floor dysfunction and psychological disorders? A cross-sectional study.

BACKGROUND: Prevalence of pelvic floor dysfunction (PFD) and its relationship with anxiety in a population undergoing physical therapy treatment in Rehabilitation Centers seems to have been little investigated in the literature. OBJECTIVE: 1) to investigate the prevalence of PFD, anxiety, depression; 2) to assess quality of life (QoL) in patients undergoing physical therapy in a Rehabilitation Center, 3) to compare the results by sex; and 4) to assess the relationship between PFD and anxiety, depression, and QoL. METHODS: This cross-sectional study included participants receiving physical therapy care in a Rehabilitation Center. Validated questionnaires were used to assess PFD, QoL, depression, and anxiety. The Chi-square test, Pearson's correlation coefficient, and a binary logistic regression model were used for data analysis. RESULTS: 253 participants (56.9% female) were included, 45% of them reported at least one PFD symptom. Females had higher prevalence of urinary incontinence (UI) (28% vs 14%); constipation (25% vs 10%); sexual dysfunction (75% vs 9%); anxiety (47% vs 35%); and depression (34% vs 17%) than males. A weak correlation was found between anxiety and depression with UI and sexual dysfunction for females. For all participants, poor QoL was found in physical functioning, physical role, bodily pain and emotional role. Being elderly (OR: 2.58 [1.24, 5.37]), partnered (OR: 1.82 [1.04, 3.17]), female (OR: 3.38 [1.91, 5.99]), and anxious (OR: 2.03 [1.14, 3.62]) were risk factors for reporting PFD. CONCLUSION: This study found a high prevalence of PFD symptoms in patients attending a Rehabilitation Center. All symptoms except fecal incontinence were more prevalent in females than in males. There was a weak correlation between UI with QoL and psychological disorders among females.

Brazilian native fruit pomace as a source of bioactive compounds on starch-based films: Antimicrobial activities and food simulator release.

The bioactive compounds extraction from fruit pomace is an ecological alternative for these abundant and low-added-value by-products. This study aimed to evaluate the antimicrobial potential of pomace extracts from Brazilian native fruits (araçá, uvaia, guabiroba and butiá) and the effect on physicochemical, mechanical properties and the migration of antioxidants and phenolic compounds from starch-based films. The film with butiá extract had the lowest mechanical resistance (1.42 MPa) but the highest elongation (63 %). In comparison, uvaia extract had less impact on film mechanical properties (3.70 MPa and 58 %) compared to the other extracts. The extracts and films showed antimicrobial activity against Listeria monocytogenes, L. inoccua, B. cereus and S. aureu. Approximately 2 cm inhibition halo was noticed for the extracts, while films ranged from 0.33 to 1.46 cm inhibition halo. Films with guabiroba extract had the lowest antimicrobial activity (0.33 to 0.5 cm). The phenolic compounds were released from the film matrix in the first hour at 4 °C with maintenance in the stability. The fatty-food simulator showed a controlled release of antioxidant compounds, which can assist in controlling food oxidation. Brazilian native fruit has shown to be a viable alternative to isolate bioactive compounds and produce film packaging with antimicrobial and antioxidant activities.

Sindrome de burnout, atividade física e fatores ocupacionais que afetam os servidores púbicos de uma universidade federal do sul do Brasil / Burnout syndrome, physical activity and occupational factors that affect public servants at a federal university in southern Brazil / Síndrome de burnout, actividad física y factores ocupacionales que afectan a los servidores públicos de una universidad federal del sur de Brasil

O estudo buscou identificar a prevalência de Síndrome de Burnout (SB), bem como verificar se existe uma associação da SB com fatores ocupacionais e a prática de atividade física (AF) em servidores técnico-administrativos (STA) de uma universidade federal do sul do Brasil. A amostra foi composta por 203 STA. Foram coletados dados sociodemográficos, ocupacionais, nutricional, SB e prática de AF. A prevalência de SB entre os STA foi de 50%, sendo que a mesma esteve associada inversamente com a faixa etária (p=0,02) e com as atividades administrativas ou de escritório (p=0,008), ambas com proteção superior a 30%. Conclui-se que a prevalência de SB é elevada entre os STA e que políticas voltadas a redução dos fatores que levam a SB entre STA devem ser criadas e implementadas pela instituição, auxiliando na redução do adoecimento (AU).

The study sought to identify the prevalence of Burnout Syndrome (BS), as well as to verify if there is an association of BS with occupational factors and the practice of physical activity (PA) in technical-administrative servers (TAS) of a federal university in south of Brazil. The sample was composed by 203 STA. Sociodemographic, occupational, nutritional, SB and PA practice data were collected. The prevalence of BS among the TAS was 50%, being that it was inversely associated with age group (p=0.02) and with administrative or desk activities (p=0.008), both with protection greater than 30 %. It was concluded that the prevalence of BS is high among the TAS and policies aimed at reducing two factors that lead to BS among TAS should be created and implemented by the institution helping in the reduction of the treatment (AU).

El estudio buscó identificar la prevalencia del Síndrome de Burnout (SB), así como verificar si existe asociación del SB con actores ocupacionales y la práctica de actividad física (AF) en servidores técnico-administrativos (STA) de una universidad federal en el sur de Brasil. La muestra fue compuesta por 203 STA. Fueron colectados datos sociodemográficos, ocupacionales, nutricional, SB y práctica de AF. La prevalencia de SB entre os STA foi de 50%, siendo que la misma estuvo asociada inversamente com el grupo de edad (p=0,02) y com las actividades administrativas o- de oficina (p=0,008), ambas con protección superior a 30 % Se concluyo que la prevalencia de SB es elevada entre los STA y políticas específicas de disminución de los factores que llevam a SB entre STA deven ser creadas y aplicadas por la institución auxiliando en la disminución de dolencias (AU).

Sindrome de burnout, atividade física e fatores ocupacionais que afetam os servidores púbicos de uma universidade federal do sul do Brasil / Burnout syndrome, physical activity and occupational factors that affect public servants at a federal university in southern Brazil / Síndrome de burnout, actividad física y factores ocupacionales que afectan a los servidores públicos de una universidad federal del sur de Brasil

O estudo buscou identificar a prevalência de Síndrome de Burnout (SB), bem como verificar se existe uma associação da SB com fatores ocupacionais e a prática de atividade física (AF) em servidores técnico-administrativos (STA) de uma universidade federal do sul do Brasil. A amostra foi composta por 203 STA. Foram coletados dados sociodemográficos, ocupacionais, nutricional, SB e prática de AF. A prevalência de SB entre os STA foi de 50%, sendo que a mesma esteve associada inversamente com a faixa etária (p=0,02) e com as atividades administrativas ou de escritório (p=0,008), ambas com proteção superior a 30%. Conclui-se que a prevalência de SB é elevada entre os STA e que políticas voltadas a redução dos fatores que levam a SB entre STA devem ser criadas e implementadas pela instituição, auxiliando na redução do adoecimento.

The study sought to identify the prevalence of Burnout Syndrome (BS), as well as to verify if there is an association of BS with occupational factors and the practice of physical activity (PA) in technical-administrative servers (TAS) of a federal university in south of Brazil. The sample was composed by 203 STA. Sociodemographic, occupational, nutritional, SB and PA practice data were collected. The prevalence of BS among the TAS was 50%, being that it was inversely associated with age group (p=0.02) and with administrative or desk activities (p=0.008), both with protection greater than 30 %. It was concluded that the prevalence of BS is high among the TAS and policies aimed at reducing two factors that lead to BS among TAS should be created and implemented by the institution helping in the reduction of the treatment.

El estudio buscó identificar la prevalencia del Síndrome de Burnout (SB), así como verificar si existe asociación del SB con factores ocupacionales y la práctica de actividad física (AF) en servidores técnico-administrativos (STA) de una universidad federal en el sur de Brasil. La muestra fue compuesta por 203 STA. Fueron colectados datos sociodemográficos, ocupacionales, nutricional, SB y práctica de AF. La prevalencia de SB entre os STA foi de 50%, siendo que la misma estuvo asociada inversamente com el grupo de edad (p=0,02) y com las actividades administrativas o- de oficina (p=0,008), ambas con protección superior a 30 % Se concluyo que la prevalencia de SB es elevada entre los STA y políticas específicas de disminución de los factores que llevam a SB entre STA deven ser creadas y aplicadas por la institución auxiliando en la disminución de dolencias.

Effects of intranasal oxytocin on pain perception among human subjects: A systematic literature review and meta-analysis.

INTRODUCTION: Oxytocin (OXT) is a peptide hormone produced in the hypothalamus that plays a neuromodulatory role in emotion, stress, and anxiety. Due to its multidimensional role, OXT is a promising target for therapeutic interventions to treat pain. OBJECTIVE: Perform a systematic literature review, followed by a meta-analysis to identify the effects of intranasal OXT on the self-perception of clinical and experimental pain among human subjects. METHOD: A systematic review was conducted in the PubMed, PsycINFO, Scielo, Lilacs, and Web of Science databases, using the keywords Oxytocin, Pain, Analgesia, and Nociception. RESULTS: Fifteen papers were included in the meta-analysis. None of the outcomes presented statistical significance in terms of the interventions' effect size: pain intensity (SMD = -0.02 (CI 95 %: -0.14 to 0.10; p = 0.76)) and pain unpleasantness (SMD = -0.15 (CI 95 %: -0.34 to 0.04; p = 0.12)). No meta-analysis was performed for pain threshold or tolerance because few papers address these outcomes. CONCLUSION: There was no statistically significant effect of intranasal OXT administration on pain perception, considering equivalence limits between (-0.2 and 0.2). However, it must be considered that the study designs may not have been sensitive enough to detect minor analgesic effects of OXT, which, being weak, may also not be perceived at a conscious level. Additionally, OXT effects possibly depend on specific characteristics of the painful condition, such as pain complexity, intensity, and duration, contextual variables like the presence of social and affective support, and individual characteristics.

New amides derived from sclareolide as anticholinesterase agents.

A series of 25 amides (15 new) derived from (3aR)-(+)-sclareolide were prepared and subjected to Ellman's assay to determine their efficacies as inhibitors for AChE or BuChE. Five amides (9, 13, 14, 15 and 17) caused inhibition of one of the enzymes greater than 60%; thereby those that inhibited BuChE were more active than positive control galantamine, and they showed better Ki values (1.07 to 8.49). In general, it was found that molecules holding a meta-substituted phenyl group showed a higher percentage of enzymatic inhibition. Molecular modelling calculations indicated the putative interactions of compounds with the amino acids residues of both enzymes AChE and BuChE. The cytotoxicity of compounds 9, 13, 14, 15 and 17 was evaluated against a non-malignant murine embryonic fibroblast cell line (NIH 3T3). Of special note is compound 15, as it presented the second-best Ki value for BuChE (1.71), was not cytotoxic (EC50 > 30 µM). Compound 15 also does not violate Lipinski rules, and showed permeability in the blood brain barrier, indicating that it can be considered a lead for the development of new drugs to treat Alzheimer's disease.

Counseling to change the lifestyle of sedentary workers on musculoskeletal pain: systematic review / Aconselhamento para mudança do estilo de vida de trabalhadores sedentários sobre a dor musculoesquelética: revisão sistemática

ABSTRACT BACKGROUND AND OBJECTIVES: Musculoskeletal pain (MSP) in sedentary workers is a cause of absenteeism, high health costs and is relate to their lifestyle and work. Systematic reviews of interventions on the condition of MSP in this population are based on work equipment and do not present consensus on the type of intervention and its effectiveness. Terefore, the objective was to analyze the evidence of intervention studies that included education strategies to change the lifestyle of sedentary workers on the reduction of MSP. CONTENTS: This systematic review follows the recommendations of PRISMA 2020. Searches were conducted until April 2021 in the PubMed, BIREME and Scielo databases, in order to identify randomized or non-randomized clinical trials published between January 1999 and April 2021. Indexed search descriptors were used and eligibility criteria were defined according to the PICOS strategy. The risk of bias was assessed using the PEDro scale. Eight randomized clinical trials published between 2004 and 2020, conducted in Europe, Asia, the United States and Australia involving 1,871 people (35 to 52 years old) were included. Interventions ranged from two weeks to 12 months. Five studies showed a higher number of women. In addition to lifestyle counseling, three studies addressed work characteristics (time in sitting posture, body posture) and three others investigated issues related to pain (symptoms, neck/shoulder anatomy and self-management). Six interventions were effective to reduce the intensity and frequency of MSP in the cervical and lumbar regions of the spine, shoulders and thoracic spine, which used counseling to increase the practice of physical activity, stress control, healthy eating, decreased alcohol consumption and smoking. Six studies presented medium/low bias risk in the following items: occult allocation, baseline comparability, blinding (individuals, therapists and evaluators), adequate follow-up and intention to treat analysis; and two studies presented medium/high risk in the same items, except in baseline comparability. CONCLUSION: Workplace interventions that include education strategies and counseling for lifestyle changes are effective for reducing the intensity and frequency of MSP in sedentary workers. PROSPERO registration: CRD42022342636. HIGHLIGHTS Lifestyle education can reduce musculoskeletal pain in workers. Physical activity and stress control contribute to reducing pain intensity. The workplace is a potent environment for decreasing musculoskeletal pain.

RESUMO JUSTIFICATIVA E OBJETIVOS: A dor musculoesquelética (DME) em trabalhadores sedentários é causa de absenteísmo, custos elevados em saúde e está relacionada ao seu estilo de vida e de trabalho. Revisões sistemáticas de intervenções sobre a condição de DME nesta população baseiam-se nos equipamentos de trabalho e não apresentam consenso quanto ao tipo de intervenção e sua efetividade. Portanto, o objetivo foi analisar as evidências dos estudos de intervenção que incluíram estratégias de educação para mudança do estilo de vida de trabalhadores sedentários para a redução da DME. CONTEÚDO: Esta revisão sistemática segue as recomendações do PRISMA 2020. Foram realizadas buscas até abril de 2021 nas bases de dados Pubmed, BIREME e Scielo, visando identificar estudos clínicos randomizados ou não randomizados publicados entre janeiro de 1999 e abril de 2021. Foram utilizados descritores de busca indexados e definidos critérios de elegibilidade segundo a estratégia PICOS. O risco de viés foi avaliado por meio da escala PEDro. Foram incluídos oito estudos clínicos randomizados publicados entre 2004 e 2020, realizados na Europa, Ásia, Estados Unidos e Austrália, que envolveram 1.871 pessoas (35 a 52 anos). As intervenções variaram de duas semanas a 12 meses. Cinco estudos apresentaram maior número de mulheres. Além dos aconselhamentos para estilo de vida, três estudos abordaram características do trabalho (tempo na postura sentada, postura corporal) e outros três investigaram questões relacionadas à dor (sintomas, anatomia pescoço/ombro e autogerenciamento). Seis intervenções foram efetivas para a redução da intensidade e da frequência de DME nas regiões cervical e lombar da coluna, nos ombros e coluna torácica, as quais utilizaram aconselhamentos para aumento da prática de atividade física, controle do estresse, alimentação saudável, diminuição do consumo de álcool e do tabagismo. Seis estudos apresentaram risco de viés médio/baixo nos itens alocação oculta, comparabilidade da linha de base, cegamento (indivíduos, terapeutas e avaliadores), acompanhamento adequado e análise de intenção de tratar; e dois estudos apresentaram risco médio/alto nos mesmos itens, exceto na comparabilidade da linha de base. CONCLUSÃO: Intervenções realizadas no local de trabalho e que incluam estratégias de educação e aconselhamentos para mudanças no estilo de vida podem ser efetivas para redução da intensidade e da frequência de DME em trabalhadores sedentários. Registro PROSPERO: CRD42022342636. DESTAQUES Educação a respeito de estilo de vida pode reduzir a dor musculoesquelética de trabalhadores. Atividade física e controle do estresse contribuem para redução da intensidade de dor. O local de trabalho é um ambiente potente para melhora na dor musculoesquelética.

Fruit pomace as a promising source to obtain biocompounds with antibacterial activity.

The demand for natural compounds to replace synthetic additives has aroused the interest of different sectors of society, especially the scientific community, due to their safety, biocompatibility, biodegradability and low toxicity. Alternative sources for antimicrobial compounds have been explored, such as fruit pomace. These by-products have essential compounds in their composition with different potential for application in food and packaging. In this context, this review systematizes the use of pomace from different fruits as a source of antibacterial compounds. Also, it summarizes the extraction methods and the applications of these compounds. Grape pomace, cranberry, and apple extracts are the most explored for antibacterial control, especially against genus Listeria, Salmonella, Staphylococcus, and Escherichia. In addition, phenolic acids, anthocyanins, flavonoids, and proanthocyanins are the main compounds identified in the studied fruit pomace extracts. In the reviewed articles, the biocompounds recovery is performed by methods with the absence of high temperatures (>80 °C); in some studies, the solid-liquid extraction method at mild temperatures (<30 °C) was well explored, using ethanol and water as solvent. The use of fruit processing by-products for bacterial control highlights the possibility of favoring the three pillars of sustainability (social, economic, and environmental) in the food industry.

[Interventions with physical activity counseling for adults without diagnosed diseases - a systematic review]. / Intervenção com aconselhamento de atividade física para adultos sem doenças diagnosticadas - uma revisão sistemática.

Counseling interventions have been broadly used to modify the level of physical activity in the population. The scope of this study was to conduct a systematic review on physical activity counseling interventions. Original intervention studies with physical activity counseling as an outcome were included and published between January 2010 and April 2020 in the Medline/PubMed, BVS, and Scielo databases, in English and Portuguese, with an adult population without diagnosed diseases. Methodological quality was assessed using the PEDro scale. We found 2,152 articles and after screening, eight manuscripts remained, writtem in seven countries. Regarding the evaluation of the quality of the work, the scores vary between 4/10 and 8/10 on the PEDro scale. The measures with counseling for PA, lasted one month and were effective in seven of the eight selected studies. The studies use the following forms of counseling: calls, SMS, cell phone apps, face-to-face counseling, e-mail, materials posted on websites, handouts, booklets and telegrams. The results of this review showed that counseling for PA practice increases the PA level of healthy adults, irrespective of the heterogeneity of methods.

Intervenções com aconselhamento têm sido amplamente usadas para modificar o nível de atividade física das populações. O presente estudo teve como objetivo realizar uma revisão sistemática sobre intervenções com aconselhamento de atividade física (AF). Foram incluídos estudos originais de intervenção com aconselhamento tendo como desfecho a atividade física, publicados nas bases de dados do Medline/PubMed, BVS e SciELO entre janeiro de 2010 e abril de 2020, nos idiomas inglês e português e com população de adultos sem doenças diagnosticadas. A qualidade metodológica foi avaliada utilizando-se a escala PEDro. Foram encontrados 2.152 artigos, após a triagem restaram oito manuscritos, produzidos em sete países. Em relação à avaliação da qualidade dos trabalhos, as pontuações variaram entre 4/10 e 8/10 na escala PEDro. As intervenções com aconselhamento para AF tiveram duração de um a seis meses e foram efetivas em sete das oito pesquisas selecionadas. Os estudos utilizaram como formas de aconselhamento: ligações, SMS, aplicativo de celular, aconselhamento face a face, e-mail, materiais postados em website, apostila, cartilhas e telegrama. Os resultados desta revisão demonstraram que o aconselhamento à prática de AF aumenta o nível de AF de adultos saudáveis, mesmo com a heterogeneidade de métodos.

Intervenção com aconselhamento de atividade física para adultos sem doenças diagnosticadas - uma revisão sistemática / Interventions with physical activity counseling for adults without diagnosed diseases - a systematic review

Resumo Intervenções com aconselhamento têm sido amplamente usadas para modificar o nível de atividade física das populações. O presente estudo teve como objetivo realizar uma revisão sistemática sobre intervenções com aconselhamento de atividade física (AF). Foram incluídos estudos originais de intervenção com aconselhamento tendo como desfecho a atividade física, publicados nas bases de dados do Medline/PubMed, BVS e SciELO entre janeiro de 2010 e abril de 2020, nos idiomas inglês e português e com população de adultos sem doenças diagnosticadas. A qualidade metodológica foi avaliada utilizando-se a escala PEDro. Foram encontrados 2.152 artigos, após a triagem restaram oito manuscritos, produzidos em sete países. Em relação à avaliação da qualidade dos trabalhos, as pontuações variaram entre 4/10 e 8/10 na escala PEDro. As intervenções com aconselhamento para AF tiveram duração de um a seis meses e foram efetivas em sete das oito pesquisas selecionadas. Os estudos utilizaram como formas de aconselhamento: ligações, SMS, aplicativo de celular, aconselhamento face a face, e-mail, materiais postados em website, apostila, cartilhas e telegrama. Os resultados desta revisão demonstraram que o aconselhamento à prática de AF aumenta o nível de AF de adultos saudáveis, mesmo com a heterogeneidade de métodos.

Abstract Counseling interventions have been broadly used to modify the level of physical activity in the population. The scope of this study was to conduct a systematic review on physical activity counseling interventions. Original intervention studies with physical activity counseling as an outcome were included and published between January 2010 and April 2020 in the Medline/PubMed, BVS, and Scielo databases, in English and Portuguese, with an adult population without diagnosed diseases. Methodological quality was assessed using the PEDro scale. We found 2,152 articles and after screening, eight manuscripts remained, writtem in seven countries. Regarding the evaluation of the quality of the work, the scores vary between 4/10 and 8/10 on the PEDro scale. The measures with counseling for PA, lasted one month and were effective in seven of the eight selected studies. The studies use the following forms of counseling: calls, SMS, cell phone apps, face-to-face counseling, e-mail, materials posted on websites, handouts, booklets and telegrams. The results of this review showed that counseling for PA practice increases the PA level of healthy adults, irrespective of the heterogeneity of methods.

Spectroscopic-chemometric modeling of 80 humic acids confirms the structural pattern identity of humified organic matter despite different formation environments.

The structure of humic substances (HSs) and the humification process are critical topics for understanding the dynamics of carbon on the planet. This study aimed to assess the structural patterns of 80 humic acid (HA) samples isolated from different soils, namely, Histosols, Ferralsols, Cambisols, Mollisols, Planosols and vermicompost, by spectroscopic characterization using solid-state 13C nuclear magnetic resonance cross-polarization/magic angle spinning combined with chemometric techniques. All 80 HAs had a similar structural pattern, regardless of their source of origin, but they had different relative quantities of organic C species. The different structural amounts of the various organic C fractions generated different properties in each of the HAs. This explains why there were similarities in the HS functions but why the intensities of these functions varied among the samples from the different soil types and environments, confirming that HSs are a group of compounds with a structural identity distinct from the molecules that give rise to them. There appears to be no single definition for the humification process; therefore, for the soils from each source of origin, a specific humification process occurs that depends on the characteristics of the local environment. Humification can be understood as a process that is similar to a chemical reaction, where the key factor that determines the formation of the products is the structural characteristics of the reactants (organic substrates deposited in the soil). The degree to which the reaction progresses is governed by the reaction conditions (chemical, physical, and biological properties of the soil). The structural patterns for HSs obtained in this study justify the existence of HSs structured as self-assembled, hydrophilic and hydrophobic domains that, under certain conditions, can undergo transformations, altering the balance of organic carbon in the environment.

Environmental implications of the organic matter structure for white-rot fungus Pleurotus eryngii growth in a tropical climate.

White-rot fungi (Pleurotus eryngii) are decomposers of lignocellulosic substrates. The relationship between the structure of humified organic matter and P. eryngii growth, is poorly understood. This study aimed to evaluate the relationship between the growth and development of white-rot fungi (P. eryngii) in two structurally different sources of humified organic matter. Fungus growth and development (mycelium diameter, fresh and dry mycelium mass, mycelium density, and biological yield) were evaluated in experiments with the application of humic substances (HS) extracted from vermicompost (VC) and peat. Both HS were characterized by CP/MAS 13C NMR spectroscopy associated with chemometrics analysis. The HS present different structural characteristics, with those extracted from VC having a predominance of functionalized C-aliphatics (carbohydrates), low hydrophobicity, and a 90% proportion of cellulose/hemicellulose carbon in the composition. HS extracted from peat have a predominance of C-aromatics (lignin fragments), higher hydrophobicity, and a proportion of lignin carbon of up to 80%. The results showed that P. eryngii growth is dependent on the C-cellulosic and C-lignin balance. HS extracted from lignin-rich peat regulates the fungus growth at initial times and sometimes inhibits the biological performance. The highly cellulosic HS from VC regulate the fungus growth at later times and its biological performance.

"I am not a blue angel": Sexuality from the perspective of autistic adolescentes. / "Eu não sou um anjo azul": a sexualidade na perspectiva de adolescentes autistas.

Despite the difficulties in living their sexualities, the sexual education of autistic people is often neglected. In this sense, this research aims to identify autistic people's demands on their sexualities, in line with the neurodiversity paradigm. This qualitative research was carried out from September 2017 to October 2018, with 14 autistic children aged 15 to 17 enrolled in regular schools. Data were collected by semi-structured interviews and analyzed according to the thematic content analysis. Two analytical categories were identified: "discursive processes and the 'blue angel' imagery"; and "diversity in diversity: the sexuality of autistic people as singular processes". The results show that, while autistic people grow physically and sexually according to the typical development stages, some singularities should not be ignored. However, the construction of false beliefs encourages the denial of autistic people's sexuality. We can conclude that the establishment of effective actions of sexual education and support to the sexuality of the autistic person requires a paradigmatic change anchored in the social model of disability.

Apesar das dificuldades enfrentadas na vivência de suas sexualidades, a educação sexual de pessoas autistas costuma ser negligenciada. Neste sentido, esta pesquisa objetiva identificar as demandas de autistas sobre sua sexualidade, alinhando-se ao paradigma da neurodiversidade. Trata-se de uma pesquisa de natureza qualitativa realizada de setembro de 2017 a outubro de 2018, com 14 autistas oralizados, com idades de 15 a 17 anos, matriculados em escolas regulares. A coleta dos dados foi realizada por entrevistas semiestruturadas e avaliadas segundo a análise temática de conteúdo. Identificaram-se 2 categorias analíticas: "processos discursivos e a imagética do 'anjo azul'" e "diversidade na diversidade: a sexualidade de autistas como processos singulares". Os resultados que embora pessoas autistas se desenvolvam fisicamente e sexualmente de acordo com os estágios típicos de desenvolvimento, existem singularidades que não deveriam ser ignoradas. Todavia, a construção de falsas crenças fomenta a negação da sexualidade de pessoas autistas. Conclui-se que o estabelecimento de ações efetivas de educação sexual e de suporte à sexualidade da pessoa autista requer uma mudança paradigmática, que ancore-se no modelo social de deficiência.

"Eu não sou um anjo azul": a sexualidade na perspectiva de adolescentes autistas / "I am not a blue angel": Sexuality from the perspective of autistic adolescentes

Resumo Apesar das dificuldades enfrentadas na vivência de suas sexualidades, a educação sexual de pessoas autistas costuma ser negligenciada. Neste sentido, esta pesquisa objetiva identificar as demandas de autistas sobre sua sexualidade, alinhando-se ao paradigma da neurodiversidade. Trata-se de uma pesquisa de natureza qualitativa realizada de setembro de 2017 a outubro de 2018, com 14 autistas oralizados, com idades de 15 a 17 anos, matriculados em escolas regulares. A coleta dos dados foi realizada por entrevistas semiestruturadas e avaliadas segundo a análise temática de conteúdo. Identificaram-se 2 categorias analíticas: "processos discursivos e a imagética do 'anjo azul'" e "diversidade na diversidade: a sexualidade de autistas como processos singulares". Os resultados que embora pessoas autistas se desenvolvam fisicamente e sexualmente de acordo com os estágios típicos de desenvolvimento, existem singularidades que não deveriam ser ignoradas. Todavia, a construção de falsas crenças fomenta a negação da sexualidade de pessoas autistas. Conclui-se que o estabelecimento de ações efetivas de educação sexual e de suporte à sexualidade da pessoa autista requer uma mudança paradigmática, que ancore-se no modelo social de deficiência.

Abstract Despite the difficulties in living their sexualities, the sexual education of autistic people is often neglected. In this sense, this research aims to identify autistic people's demands on their sexualities, in line with the neurodiversity paradigm. This qualitative research was carried out from September 2017 to October 2018, with 14 autistic children aged 15 to 17 enrolled in regular schools. Data were collected by semi-structured interviews and analyzed according to the thematic content analysis. Two analytical categories were identified: "discursive processes and the 'blue angel' imagery"; and "diversity in diversity: the sexuality of autistic people as singular processes". The results show that, while autistic people grow physically and sexually according to the typical development stages, some singularities should not be ignored. However, the construction of false beliefs encourages the denial of autistic people's sexuality. We can conclude that the establishment of effective actions of sexual education and support to the sexuality of the autistic person requires a paradigmatic change anchored in the social model of disability.

Adverse childhood experiences and chronic lung diseases in adulthood: a systematic review and meta-analysis.

Background: Adverse childhood experiences (ACE) affect physical and mental health and may appear as risk factors for the development of different conditions in adult life. Objective: To perform a literature review and meta-analysis on risk indicators for the development of chronic lung diseases in adulthood associated with ACE. Method: We conducted a systematic literature review according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines using the online databases PubMed, PsycINFO, and Web of Science. Quantitative studies involving male and female adults were included. Fixed- and random-effect models were used in the estimation of meta-analytical measures. The heterogeneity between studies was assessed using I2 statistics and Cochran's Q test. Results: A total of 19 studies were selected for the meta-analysis. The analyses showed statistically significant associations between ACE and lung diseases in general (OR = 1.41; CI 95%: 1.28-1.54), besides specific associations with asthma (OR = 1.32; CI 95%: 1.13-1.50) and COPD (OR = 1.44; CI 95%: 1.13-1.76). When the mediating effect of smoking was assessed separately we found a significant - although not quite expressive - association (OR = 1.06; CI 95%: 1.02 to 1.10), which weakens the hypothesis that a direct relationship exists between childhood trauma and the occurrence of lung diseases. Conclusions: ACE are an important risk factor for the development of lung diseases in adulthood, whether through direct or indirect contribution to this outcome, which highlights the relevance of increasing the awareness of health staff for the early detection and intervention in situations of vulnerability or risk in childhood as an important preventative measure.

Antecedentes: Experiencias infantiles adversas (ACE) afectan la salud física y mental y pueden aparecer como factores de riesgo para el desarrollo de diferentes afecciones en la vida adulta.Objetivo: realizar una revisión de la literatura y un metanálisis sobre indicadores de riesgo para el desarrollo de enfermedades pulmonares crónicas en la edad adulta asociadas con ACE.Método: Realizamos una revisión sistemática de la literatura de acuerdo con las pautas PRISMA (Elementos de Referencia para Revisiones Sistemáticas y Metaanálisis) utilizando las bases de datos en línea PubMed, PsycINFO y Web of Science. Se incluyeron estudios cuantitativos con hombres y mujeres adultos. Se utilizaron modelos de efectos fijos y aleatorios en la estimación de medidas meta-analíticas. La heterogeneidad entre los estudios se evaluó mediante estadísticas I 2 y la prueba Q de Cochran.Resultados: Se seleccionaron un total de 19 estudios para el metanálisis. Los análisis mostraron asociaciones estadísticamente significativas entre el ACE y las enfermedades pulmonares en general (OR = 1.41; IC 95%: 1.28­1.54), además de asociaciones específicas con el asma (OR = 1.32; IC 95%: 1.13 ­ 1.50) y EPOC (OR = 1,44; IC 95%: 1,13­1,76). Cuando el efecto mediador del tabaquismo se evaluó por separado, encontramos una asociación significativa (aunque no del todo clara) (OR = 1.06; IC 95%: 1.02 a 1.10), lo que debilita la hipótesis de que existe una relación directa entre el trauma infantil y la ocurrencia de enfermedades pulmonares.Conclusiónes: las experiencias ACE son un factor de riesgo importante para el desarrollo de enfermedades pulmonares en la edad adulta, ya sea a través de una contribución directa o indirecta, lo que resalta la relevancia de aumentar la conciencia del personal de salud para la detección temprana y la intervención en situaciones de vulnerabilidad o riesgo en la infancia como una medida preventiva importante.

O espaço da ética e bioética na graduação em medicina / The teaching of ethics and bioethics in Medical Schools / El espacio de la ética y bioética en la graduación de Medicina

OBJETIVO: Verificar como são ofertados os conteúdos sobre ética e bioética nos currículos de formação médica das Universidades do Ceará. MÉTODOS: Trata-se de pesquisa documental, realizada em julho de 2017 abrangendo quatro Universidades que ofertam o curso de Medicina, foram elas: Universidade de Fortaleza (UNIFOR); Universidade Federal do Ceará (UFC); Universidade Estadual do Ceará (UECE) e Universidade Christus (Unichristus). A coleta dos dados se deu por meio de revisão das matrizes curriculares dos cursos, que foram obtidas através de pesquisa nos sites das universidades ou disponibilizadas pelas coordenações dos respectivos cursos. Nesses documentos, foram investigados o caráter da oferta, a carga horária e as disciplinas específicas que abordavam os conteúdos de ética e bioética, assim como se estavam presentes nos programas pedagógicos das diversas disciplinas do curso, para averiguar a transversalidade desses temas. RESULTADOS: Após avaliação das matrizes curriculares das quatro universidades e dos projetos pedagógicos de duas instituições de ensino analisadas, observou-se que as temáticas ética e bioética foram citadas por todas como parte integrante da formação médica em seus currículos, ainda que de maneira não uniforme entre elas. CONCLUSÃO: Os conteúdos de ética e bioética estão presentes nas matrizes curriculares dos quatro cursos de medicina do Ceará, quer seja no formato de disciplinas específicas, quer seja abordando o tema em disciplinas específicas que, dentre outros assuntos, abordam também a temática ética e bioética.

OBJECTIVE: To assess how ethics and bioethics contents are included in the curricula of medical schools of universities in Ceará. METHODS: This documentary research was carried out in July 2017 in four universities that have medical schools: University of Fortaleza (Universidade de Fortaleza ­ Unifor), Federal University of Ceará (Universidade Federal do Ceará ­ UFC), Ceará State University (Universidade Estadual do Ceará ­ UECE) and Christus University (Universidade Christus ­ Unichristus). Data were collected by reviewing the curricula of the schools provided by their coordination offices or found on the websites of the universities. The documents were analyzed in terms of the nature of the content, the number of hours and specific courses that addressed ethics and bioethics. We also checked if the contents were included in the pedagogical planning of the other courses taught in the school to check for cross-cutting themes. RESULTS: After analyzing the curricula of the four universities and the pedagogical projects of two universities, we found that ethics and bioethics were cited in the curricula of all the institutions as an integral part of medical education, although such inclusion did not occur in a uniform manner across the universities. CONCLUSION: Ethics and bioethics contents are present in the curricula of medical schools in Ceará, either in specific courses or in courses that cover, among other themes, ethics and bioethics.

OBJETIVO: Verificar como se ofrecen los contenidos de ética y bioética en los currículums de formación médica de las Universidades de Ceará. MÉTODOS: Se trata de una investigación documental realizada en julio de 2017 en cuatro universidades que tienen el curso de Medicina: Universidad de Fortaleza (UNIFOR); Universidad Federal de Ceará (UFC); Universidad Estadual de Ceará (UECE) y Universidad Christus (Unichristus). La recogida de datos se dio con la revisión de las matrices de los currículums de los cursos a través de una investigación en las páginas web de las universidades o aquellas disponibles en las coordinaciones de los respectivos cursos. Se investigaron en los documentos el carácter de la oferta, la carga horaria y las disciplinas específicas que tenían los contenidos de ética y bioética así como si estaban en los programas pedagógicos de las diversas disciplinas del curso para verificar la transversalidad de eses temas. RESULTADOS: Después de la evaluación de las matrices curriculares de las cuatro universidades y de los proyectos pedagógicos de dos instituciones de educación analizadas se observó que los temas ética y bioética han sido citados por todas como parte integrante de la formación médica en sus currículums aunque de manera no uniforme entre ellas. CONCLUSIÓN: Los contenidos de ética y bioética están en las matrices curriculares de los cuatro cursos de medicina de Ceará en formato de disciplinas específicas o en disciplinas que incluyen también los temas de ética y bioética.