Genome-wide identification of the HSP70 genes in Pacific oyster Magallana gigas and their response to heat stress.

Heat shock protein 70 (HSP70), the most prominent and well-characterized stress protein in animals, plays an important role in assisting animals in responding to various adverse conditions. In the present study, a total of 113 HSP70 gene family members were identified in the updated genome of Magallana gigas (designated MgHSP70) (previously known as Crassostrea gigas). There were 75, 12, 11, and 8 HSP70s located in the cytoplasm, nucleus, mitochondria, and endoplasmic reticulum, respectively, and 7 HSP70s were located in both the nucleus and cytoplasm. Among 113 MgHSP70 genes, 107 were unevenly distributed in 8 chromosomes of M. gigas with the greatest number in chromosome 07 (61 genes, 57.01%). The MgHSP70 gene family members were mainly assigned into five clusters, among which the HSPa12 subfamily underwent lineage-specific expansion, consisting of 89 members. A total of 68 MgHSP70 genes (60.18%) were tandemly duplicated and formed 30 gene pairs, among which 14 gene pairs were under strong positive selection. In general, the expression of MgHSP70s was tissue-specific, with the highest expression in labial palp and gill and the lowest expression in adductor muscle and hemocytes. There were 35, 31, and 47 significantly upregulated genes at 6, 12, and 24 h after heat shock treatment (28 °C), respectively. The expression patterns of different tandemly duplicated genes exhibited distinct characteristics after shock treatment, indicating that these genes may have different functions. Nevertheless, genes within the same tandemly duplicated group exhibit similar expression patterns. Most of the tandemly duplicated HSP70 gene pairs showed the highest expression levels at 24 h. This study provides a comprehensive description of the MgHSP70 gene family in M. gigas and offers valuable insights into the functions of HSP70 in the mollusc adaptation of oysters to environmental stress.

Polymeric microlens array formed on a discontinuous wetting surface using a self-assembly technique.

In this paper, we demonstrate a facile way to prepare polymeric microlens arrays (MLAs) based on a discontinuous wetting surface using a self-assembly technique. A patterned hydrophobic-octadecyltrichlorosilane (OTS) surface was prepared by U V/O 3 irradiation through a shadow mask. The area exposed to U V/O 3 irradiation turned highly hydrophilic, whereas the area protected by the mask remained highly hydrophobic, generating the patterned OTS surface. The surface energy of the OTS/glass surface changed from 23 to 72.8 mN/m after 17 min of U V/O 3 treatment. The scribing of the optical glue-NOA 81 onto the microhole array enabled one to obtain the MLAs due to the generation of the NOA 81 droplet array via the surface tension. After UV light curing, the cured NOA 81 droplet array with uniform dimensions within a large area exhibited excellent MLA characteristics. Moreover, the method developed in this study is simple in operation, low-cost, and requires neither a clean room nor expensive equipment.

Dielectric Liquid Microlens Array with Tunable Focal Length Based on Microdroplet Array Created via Dip-Coating Method.

A dielectric liquid microlens array (LMA) with a tunable focal length was fabricated by using a microdroplet array generated through the dip-coating method. The process began with treating the octadecyltrichlorosilane (OTS) layer with selective UV/O3 irradiation for 20 min to establish a hydrophilic-hydrophobic patterning surface. The substrate was subsequently immersed in glycerol and then withdrawn at a constant rate to create a microdroplet array. Upon filling the cell with matching oil (SL5267) and placing it within a square array of a 200 µm diameter glycerol microdroplet array, the LMA was produced. The focal length ranged from approximately -0.96 to -0.3 mm within a voltage range of 0 to 60 Vrms. The glycerol microdroplets, characterized by their shapes, sizes, curvatures, and filling factors, can be precisely controlled by designing an OTS patterning or adjusting the dip-coating speed. This approach offers a rapid and high-throughput method for preparation. Our approach to fabricating tunable LMA offers several advantages, including simplicity of fabrication, uniform structural properties, cost-effectiveness, polarization independence, and excellent optical performance. These focus-tunable LMAs hold significant potential for applications in image processing, 3D displays, medical endoscopy, and military technologies.

Modified Lignin-Based Cement Solidifying Material for Improving Engineering Residual Soil.

Although lignin improves the strength and modulus of soil, it is less active when unmodified, and it exhibits more limited effects on soils in combination with traditional Ca-based curing agents. Lignin-solidified soil also exhibits deficiencies, such as poor durability under dry-wet cycling conditions, and thus, the amelioration effect is limited. This study investigated the enhancement of cement-solidified soil using hydroxylated lignin with sodium silicate and quicklime used as activators to improve the engineering performance and durability of the treated soil. Using respective cement, sodium silicate, quicklime, and lignin contents of 7%, 0.4%, 0.2%, and 0.2% with respect to the dry mass of the slag soil, the strength and cohesion of the composite-solidified soil were 1.5 times those of cement-solidified soil, whereas the internal friction angle increased by 5.1°. At a solidifying age of 14 d, the penetration resistance almost doubled, indicating a significant improvement in the bearing capacity of the soil. The results suggest that modified lignin-based admixtures may significantly enhance the performance of cement-solidified soil. The cement curing admixture used in this study provides theoretical and technological support for curing agent preparation and the utilization of slag.

Electrically controllable broadband reflective linear cross-polarization conversion based on liquid crystals.

This paper presents an electrically controllable reflective broadband linear polarization (LP) converter based on liquid crystals (LCs) for cross-polarization conversion (CPC) in the terahertz frequency range. The proposed structure achieves a high polarization conversion ratio (PCR) exceeding 0.9 within the frequency range of 236.8 - 269.6 GHz. A vital feature of this design is the dynamic control of polarization conversion by re-orienting the nematic liquid crystal molecules through voltage bias switching between 'on' and 'off' states, allowing for precise manipulation of cross-polarized and co-polarized reflected waves. Experimental results validate the simulation outcomes, demonstrating excellent agreement. In contrast to conventional reflective polarization converters with fixed frequency responses, the proposed electrically controllable polarization conversion offers significant potential in imaging and optical communications.

Multi-stable dye-doped dual-frequency twisted nematic liquid crystal smart window.

In this study, a novel, to the best of our knowledge, dye-doped dual-frequency liquid crystal multi-stable smart window is proposed. A chiral dopant with appropriate content was introduced into dye-doped dual-frequency liquid crystals to achieve an initial 180° twisted state. These liquid crystals can be switched to a nematic phase or a 360° twisted state by controlling the magnitude and frequency of the applied voltage. These nematic phase and 360° twisted states can exist stably for a long time because of the backflow effect and the anisotropic nature of the dual-frequency liquid crystal material. Due to the optical waveguide effect of dye-doped liquid crystals in the long-pitch state, the transmittance was different in nematic phase, 180°, and 360° twisted three zero-field stabilized absorption states. Finally, a multi-stable smart window is developed to switch between three zero-field stabilized absorption and scattering states.

Manipulation of sub-terahertz waves using digital coding metasurfaces based on liquid crystals.

This paper presents a novel sub-terahertz liquid crystal (LC) phase shifter based on digital coding metasurfaces. The proposed structure consists of metal gratings and resonant structures. They are both immersed in LC. The metal gratings function as reflective surfaces for electromagnetic waves and electrodes for controlling the LC layer. The proposed structure changes the state of the phase shifter by switching the voltage on every grating. It allows the deflection of LC molecules within a subregion of the metasurface structure. Four switchable coding states of the phase shifter are obtained experimentally. The phase of the reflected wave varies by 0°, 102°, 166°, and 233° at 120 GHz. Due to the presence of the transverse control electric field, modulation speed is approximately doubled compared to the free relaxation state. This work provides a novel idea for wavefront modulation of phase.

Exploring impacts of water-extractable organic matter on pre-ozonation followed by nanofiltration process: Insights from pH variations on DBPs formation.

This study investigated the influence of pH (4-10) on the treatment of water-extractable organic matter (WEOM), and the associated disinfection by-products (DBPs) formation potential (FP), during the pre-ozonation/nanofiltration treatment process. At alkaline pH (9-10), a rapid decline in water flux (> 50 %) and higher membrane rejection was observed, as a consequence of the increased electrostatic repulsion forces between the membrane surface and organic species. Parallel factor analysis (PARAFAC) modeling and size exclusion chromatography (SEC) provides detailed insights into the WEOM compositional behavior at different pH levels. Ozonation at higher pH significantly reduced the apparent molecular weight (MW) of WEOM in the 4000-7000 Da range by transforming the large MW (humic-like) substances into small hydrophilic fractions. Fluorescence components C1 (humic-like) and C2 (fulvic-like) exhibited a predominant increase/decrease in concentration for all pH conditions during pre-ozonation and nanofiltration treatment process, however, the C3 (protein-like) component was found highly associated with the reversible and irreversible membrane foulants. The ratio C1/C2 provided a strong correlation with the formation of total trihalomethanes (THMs) (R2 = 0.9277) and total haloacetic acids (HAAs) (R2 = 0.5796). The formation potential of THMs increased, and HAAs decreased, with the increase of feed water pH. Ozonation markedly reduced the formation of THMs by up to 40 % at higher pH levels, but increased the formation of brominated-HAAs by shifting the formation potential of DBPs towards brominated precursors.

Discovery and characterization of a selective IKZF2 glue degrader for cancer immunotherapy.

Malignant tumors can evade destruction by the immune system by attracting immune-suppressive regulatory T cells (Treg) cells. The IKZF2 (Helios) transcription factor plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice. Here we report the discovery of NVP-DKY709, a selective molecular glue degrader of IKZF2 that spares IKZF1/3. We describe the recruitment-guided medicinal chemistry campaign leading to NVP-DKY709 that redirected the degradation selectivity of cereblon (CRBN) binders from IKZF1 toward IKZF2. Selectivity of NVP-DKY709 for IKZF2 was rationalized by analyzing the DDB1:CRBN:NVP-DKY709:IKZF2(ZF2 or ZF2-3) ternary complex X-ray structures. Exposure to NVP-DKY709 reduced the suppressive activity of human Treg cells and rescued cytokine production in exhausted T-effector cells. In vivo, treatment with NVP-DKY709 delayed tumor growth in mice with a humanized immune system and enhanced immunization responses in cynomolgus monkeys. NVP-DKY709 is being investigated in the clinic as an immune-enhancing agent for cancer immunotherapy.

Large-Area and Rapid Fabrication of a Microlens Array on a Flexible Substrate for an Integral Imaging 3D Display.

A curved integral imaging three-dimensional (3D) display attracts a lot of interest due to its enhanced 3D sense of immersion and wider viewing angle. In this paper, a microlens array (MLA) based on a flexible poly(ethylene terephthalate) (PET) substrate was achieved by a straightforward, rapid, and low-cost technique. The reactive ion etching (RIE) process treated PET/CYTOP covered with a flexible mask to generate a hydrophilic-hydrophobic patterned surface. The well-designed arrays of confined adhesive droplets with a controlled geometry on a hydrophilic-hydrophobic patterned surface were formed using the blade-coating method. A flexible MLA with a diameter of 820 µm, a size of 5.3 cm × 5.1 cm, and a radius of curvature of 25 cm was fabricated and combined with a curved two-dimensional (2D) monitor to realize a lateral viewing range of 6.4 cm at a viewing distance of 25 cm, which is 4 times larger than with flat integral imaging 3D display system. The flexible MLA has the advantages of a controllable lens profile and large pitch, and it can be manufactured on a large scale. In addition, it provides a large viewing angle for the reconstructed 3D image.

Empirical analysis of the impact of China-Japan-South Korea transportation infrastructure investment on environmental degradation and the validity of the environmental Kuznets curve hypothesis.

Building sustainable and affordable transport systems is a key issue for social development and sustainable urban expansion. The study used dynamic ordinary least squares (DOLS) and fully modified ordinary least squares (FMOLS) to examine the impact of transport infrastructure investment on environmental degradation in China, Japan, and South Korea over the period 1995-2020 and the validity of the EKC hypothesis. The results show that GDP has a significant positive effect, and GDP2 and GDP3 have significant adverse effects on environmental degradation, respectively. These results confirm the validity of the inverted U shaped EKC hypothesis in selected Asian countries. Road infrastructure investment has a significant positive effect, while railway infrastructure investment has a significant adverse effect on environmental degradation. Air infrastructure investment and trade opening have a progressive and statistically significant impact on environmental pollution. Modern rail systems that run on electricity are considered less polluting, so the share of rail infrastructure investment in the transport mix can help build sustainable and safe transport systems at the city Centre and intercity levels and reduce emissions in Asian countries. Moreover, strict enforcement of the prevailing environmental conditions of trade agreements should be encouraged to reduce the increasing impact of free trade on environmental pollution.

Liquid crystal-based wide-angle metasurface absorber with large frequency tunability and low voltage.

A tunable metasurface absorber (MA) based on polymer network liquid crystal is introduced in this paper. Despite the well-designed unit cell patterns, the proposed MA can achieve both large frequency tunability and wide-angle stability. Compared with traditional liquid crystal-based metasurfaces, the measured results suggest that the recovery time of the proposed structure was reduced by half. By applying an external voltage on the top electrode of the liquid crystal layer from 0 to a saturation voltage of 10 V, the absorption peak of the MA can be tuned from 112.7 GHz to 102.2 GHz, with a maximum frequency tunability of 9.3%, which is significantly higher than other proposed liquid crystal-based metasurfaces. Moreover, the proposed tunable absorber can maintain absorption greater than 90% with incident angles reaching up to 60° for both transverse electric and transverse magnetic polarizations. This design provides an efficient way for developing low-power consumption terahertz devices with large frequency tunability and wide-angle stability.

Fabrication of a bionic compound eye on a curved surface by using a self-assembly technique.

Microlens arrays on curved surfaces are regarded as critical elements of bionic compound eyes (BCEs), which exhibit the comparative advantages of a wide field of view and tracking fast-moving objects. However, the fabrication of a curved microlens array is still challenging. Along these lines, in this work, a straightforward, rapid, and low-cost technique for the fabrication of curved microlens arrays is reported by using the self-assembly technique. A reactive ion etching process treated the surface of the curved polydimethylsiloxane (PDMS) substrate to generate a hydrophobic-hydrophilic pattern. Then, the curved microlens array can be realized by dewetting a liquid glue onto the substrate using the dip-coating method and followed by crosslinking. The proposed BCE structure consists of 2400 microlenses (400 - µm diameter and 440 - µm center distance) arranged in a hexagonal configuration on a curved PDMS surface (34 - mm diameter and 40.4 - mm curvature radius). A field-of-view of 50° was demonstrated, which has potential applications in various fields including imaging sensors, medical diagnostics, machine vision systems, and photodetectors.

Rapid terahertz wave manipulation in a liquid-crystal-integrated metasurface structure.

A terahertz phase shifter based on liquid-crystal-integrated metasurface is proposed, which contains a three-slotted array structure and comb grating. The orientation of the liquid crystal molecules can be completely controlled by the direction of the electric field. From the acquired experimental results, it was demonstrated that the phase shift exceeds 300° in the range of 378.6 - 390.8 GHz, whereas the maximum phase shift reaches 374.1° at 383.1 GHz. The molecular reorientation transient process induced by the external electric field in the liquid crystal was measured and analyzed. Based on the molecular reorientation mechanism, which can be divided into three processes, a rapid modulation mechanism was demonstrated. From the performance of the proposed device, an actively controllable phase delay and reflectance with a cycle switching time of approximately 0.3 s was achieved, which is remarkably faster than the usual cycle time that exceeds 8 s. Our work provides useful ideas for improving the response speed of LC-based terahertz devices, which is considered of great significance for several applications, in terms of terahertz reconfigurable devices.

Electrically active control of terahertz waves in a hybrid metasurface-grating structure with a liquid crystal.

A reflective terahertz phase shifter for wide-range dynamic and continuous phase modulation is proposed. By injecting a tunable liquid crystal between the slotted metasurface and the grating microstructure, the phases of reflected waves can be modulated with different electrically driven methods. Numerical simulations show that the proposed terahertz phase shifter has a phase difference of more than 360° between unbiased and biased states. Furthermore, an array of 35×35 patch elements was designed and fabricated. The performance of the phase shifter provides more than 360° between 379.6 and 391.8 GHz, where the maximum phase shift reaches 422.4° at 385.9 GHz. Moreover, the fully electrically controlled phase modulation of more than 180° is achieved between 382.0 and 394.1 GHz, with a maximum phase modulation of 248.4° at 383.3 GHz. This work may provide a reflective terahertz phase modulator for beam steering.

Highly efficient and tunable terahertz polarization converter based on double subwavelength metallic gratings infiltrated with liquid crystal.

In this work, a tunable cross-polarized transmission structure at the terahertz frequency was demonstrated, and the polarization state during modulation was investigated. The proposed structure can significantly enhance the polarization conversion performance of nematic liquid crystals by leveraging the formation of a Fabry-Perot-like resonant cavity that consists of two metal gratings. As a result, the incident waves are continuously reflected in the liquid crystal layer to complete the accumulation of polarization angle changes. From the experimental results acquired, it was concluded that the insertion loss of the cross-polarization transmission was less than 3 dB and the extinction ratio was larger than 28 dB in the frequency range of 388-426 GHz. Our work provides useful insights for improving the efficiency of cross-polarization conversion by enhancing the resonance process in a Fabry-Perot-like resonant cavity and, thus, significantly extending the equivalent optical path.

Active continuous control of terahertz wave based on a reflectarray element-liquid crystal-grating electrode hybrid structure.

In this work, a new and efficient terahertz reflective phase shifter is proposed. The phase shifter is composed of a metal-dielectric-metal structure with a double dipole patch array, as well as copper grating electrodes immersed within the nematic liquid crystal. More specifically, the employed copper grating electrodes consist of two sets of cross-distributed comb grids, whereas at each set of comb grids can be applied an external bias voltage separately. On top of that, the electric field in the liquid crystal (LC) layer can be continuously changed by enforcing an innovative technique. Consequently, the orientation of the LC molecules was fully controlled by the applied electric field, since the dielectric constant of the LC is controlled by the biased voltage. The phase of the reflective electromagnetic wave can be continuously manipulated. Under this direction, the experimental results show that the phase shift exceeds the value of 180° in the range of 102.5 GHz-104.3 GHz, where the maximum phase shift is 249° at 103 GHz. The proposed work provides a new regulation concept for the implementation of LC-based terahertz devices and the respective applications in the terahertz reconfigurable antennas field.

Characterization of sabatolimab, a novel immunotherapy with immuno-myeloid activity directed against TIM-3 receptor.

Objectives: Sabatolimab is a humanized monoclonal antibody (hIgG4, S228P) directed against human T-cell immunoglobulin domain and mucin domain-3 (TIM-3). Herein, we describe the development and characterization of sabatolimab. Methods: Sabatolimab was tested for binding to its target TIM-3 and blocking properties. The functional effects of sabatolimab were tested in T-cell killing and myeloid cell cytokine assays. Antibody-mediated cell phagocytosis (ADCP) by sabatolimab was also assessed. Results: Sabatolimab was shown to (i) enhance T-cell killing and inflammatory cytokine production by dendritic cells (DCs); (ii) facilitate the phagocytic uptake of TIM-3-expressing target cells; and (iii) block the interaction between TIM-3 and its ligands PtdSer/galectin-9. Conclusion: Taken together, our results support both direct anti-leukemic effects and immune-mediated modulation by sabatolimab, reinforcing the notion that sabatolimab represents a novel immunotherapy with immuno-myeloid activity, holding promise for the treatment of myeloid cell neoplasms.

Study of the Fluidized Separation Process of Irregular Coarse-Grained Pulverized Coal Agglomerates Based on Modified Computational Fluid Dynamics-Population Balance Method.

In this work, the agglomeration, fragmentation, and separation process of coarse-grained pulverized coal agglomerates (CGPCA) obtained from a power plant were achieved using gas-solid fluidized bed sorting and analyzed through a combination of numerical simulations and actual experiments with CGPCA. To study the polydispersity and irregularity of CGPCA, the CGPCA surface fractal dimension was calculated using fractal dimension combined with scanning electron microscopy. The two-section fractal dimension of the particle size distribution was obtained by fitting the logarithmic particle size distribution of CGPCA. On the basis of the obtained data, the polydisperse particle drag force model, the agglomeration kernel function, and the breakage kernel function were modified. Thus, an irregular polydisperse gas-solid two-phase Eulerian-Eulerian model was constructed to simulate the sorting process of CGPCA in the fluidized bed. The results of the numerical simulation investigation were compared with the experimental results and showed that the simulation data, which considered the two section fractal dimension, was in better agreement with the experimental results. The cumulative logarithmic distribution of CGPCA's size was segmented and fitted. The values of the two section fractals of the agglomerates were determined as D = 1.014 and D = 2.401, respectively. Analysis revealed that the optimal separation efficiency working condition in the simulation process, providing the highest separation efficiency of 54.7%, was generated at air velocity of 1.21 m/s.

Stable and tunable single-mode lasers based on cholesteric liquid crystal microdroplets.

Although many studies on cholesteric liquid crystal (CLC) microdroplet single-mode lasers are available, it has been shown that the stability and tunability of such microdroplets are difficult to achieve simultaneously. In this paper, a new, to the best of our knowledge, method is proposed for the mass and rapid preparation of stable and tunable monodisperse CLC microdroplet single-mode lasers. This is based on the formation of polymer networks on the surface of the microdroplet via interfacial polymerization and a disruption of the orderliness of the polymer networks by increasing the temperature during polymerization, which results in a single pitch inside the microdroplets. This approach enables CLC microdroplet single-mode lasers to achieve improved environmental robustness, while maintaining the same temperature tunability as the unpolymerized sample. Our method has promising future applications in integrated optics, flexible devices, and sensors.