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1.
Opt Express ; 29(20): 32312-32324, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34615305

RESUMO

The ultrafast process by the electron in molecular ions from one site or region to another that has come to be known as charge migration (CM), which is of fundamental importance to photon induced chemical or physical reactions. In this work, we study the electron current and ultrafast magnetic-field generation based on CM process of oriented asymmetric (HeH2+) and symmetric (H2 +) molecular ions. Calculated results show that they are ascribed to quantum interference of electronic states for these molecular ions under intense circularly polarized (CP) laser pulses. The two scenarios of (i) resonance excitation and (ii) direct ionization are considered through appropriately utilizing designed laser pulses. By comparison, the magnetic field induced by the scenario (i) is stronger than that of scenario (ii) for molecular ions. However, the scheme (ii) is very sensitive to the helicity of CP field, which is opposite to the scenario (i). Moreover, the magnetic field generated by H2 + is stronger than that by HeH2+ through scenario (i). Our findings provide a guiding principle for producing ultrafast magnetic fields in molecular systems for future research in ultrafast magneto-optics.

2.
Mol Biol Rep ; 38(3): 1989-94, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20857214

RESUMO

The cDNA code of thioredoxin h, designated as HbTRX1, was isolated from Hevea brasiliensis by rapid amplification of cDNA ends. HbTRX1 contained a 542-bp open reading frame encoding 123 amino acids. The deduced HbTRX1 protein showing high identity to thioredoxin h of other plant species was predicted to possess the conserved catalytic site WCXPC. Semiquantitative reverse transcription-polymerase chain reaction analysis revealed that HbTRX1 was constitutively expressed in all tested tissues. HbTRX1 transcripts accumulated at relatively low levels in the flower, somatic embryo, and leaves, while HbTRX1 transcripts accumulated at relatively high levels in the callus and latex. The HbTRX1 transcript was expressed at different levels, with higher levels in self-rooting juvenile clones than in their donor clones. HbTRX1 was expressed in Escherichia coli, and its activity was demonstrated using the dithiothreitol-dependent insulin assay. This work provides a basis for studying the biological function of thioredoxin h in rubber tree.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Hevea/genética , Látex/metabolismo , Raízes de Plantas/genética , Tiorredoxina h/genética , Células Clonais , Clonagem Molecular , Ensaios Enzimáticos , Escherichia coli , Dados de Sequência Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regiões Promotoras Genéticas/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Tiorredoxina h/isolamento & purificação , Tiorredoxina h/metabolismo , Transcrição Gênica
3.
Int J Syst Evol Microbiol ; 54(Pt 6): 2431-2435, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15545492

RESUMO

In an investigation of the yeast biota involved in silage deterioration, a considerable number of strains belonging to Saccharomyces and related genera were isolated from aerobically deteriorating corn silage in Tochigi, Japan. Analysis of sequences of the internal transcribed spacer and the large-subunit rRNA gene D1/D2 domain and electrophoretic karyotyping indicated that two of the strains, NS 14(T) and NS 26, represent a novel species with close phylogenetic relationships to Kazachstania servazzii and Kazachstania unispora. It is proposed that the novel species be named Kazachstania aerobia sp. nov., with NS 14(T) (=AS 2.2384(T)=CBS 9918(T)) as the type strain.


Assuntos
Saccharomycetales/classificação , Saccharomycetales/isolamento & purificação , Silagem/microbiologia , Zea mays/microbiologia , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Ribossômico/química , DNA Ribossômico/isolamento & purificação , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/isolamento & purificação , Genes de RNAr , Japão , Cariotipagem , Dados de Sequência Molecular , Filogenia , RNA Fúngico/genética , Saccharomycetales/citologia , Saccharomycetales/genética , Análise de Sequência de DNA
4.
Int J Syst Evol Microbiol ; 54(Pt 5): 1877-1882, 2004 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-15388757

RESUMO

Among yeasts isolated from plant leaves collected in different regions of China that form whitish or yellowish colonies and symmetrical ballistoconidia, four strains were shown to represent three novel Bullera species by conventional and molecular taxonomic characterization. The novel species are described as Bullera cylindrica sp. nov. (type strain CB 169T = AS 2.2308T = CBS 9744T), Bullera hubeiensis sp. nov. (type strain HX 19.3T = AS 2.2466T = CBS 9747T) and Bullera nakasei sp. nov. (type strain HX 15.5T = AS 2.2435T = CBS 9746T). These three species, and another eight previously described Bullera species represented by Bullera mrakii, formed a strongly supported distinct clade among the hymenomycetous yeasts in each of the phylogenetic trees drawn from the 26S rDNA D1/D2 domain and the internal transcribed spacer region sequences.


Assuntos
Basidiomycota/classificação , Basidiomycota/isolamento & purificação , Folhas de Planta/microbiologia , Basidiomycota/citologia , Basidiomycota/genética , China , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Ribossômico/química , DNA Ribossômico/isolamento & purificação , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/isolamento & purificação , Genes de RNAr , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , RNA Fúngico/genética , RNA Ribossômico/genética , Análise de Sequência de DNA
5.
Int J Syst Evol Microbiol ; 54(Pt 4): 1409-1414, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15280322

RESUMO

Among ascomycetous yeasts that were isolated from several nature reserve areas in China, three anamorphic strains isolated from soil (QL 5-5T) and fruit (QL 21-2T and SN 15-1T) were revealed, by conventional characterization and molecular phylogenetic analysis based on internal transcribed spacer and large subunit (26S) rRNA gene D1/D2 region sequencing, to represent three novel species in the genus Candida. Candida qinlingensis sp. nov. (type strain, QL 5-5T=AS 2.2524T=CBS 9768T) was related closely to a teleomorphic species, Williopsis pratensis. The close relatives of Candida diospyri sp. nov. (type strain, QL 21-2T=AS 2.2525T=CBS 9769T) are Candida friedrichii and Candida membranifaciens. Candida asparagi sp. nov. (type strain, SN 15-1T=AS 2.2526T=CBS 9770T) forms a clade with Candida fructus.


Assuntos
Candida/classificação , Candida/isolamento & purificação , Diospyros/microbiologia , Microbiologia do Solo , Candida/citologia , Candida/genética , China , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , DNA Ribossômico/química , DNA Espaçador Ribossômico/genética , Microbiologia de Alimentos , Frutas/microbiologia , Genes de RNAr , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , RNA Fúngico/genética , RNA Ribossômico/genética , Análise de Sequência de DNA , Homologia de Sequência
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