RESUMO
Mitochondrial dysfunction and metabolic disorder have been associated to age-related subfertility, however, the precise molecular mechanism controlling the development of fertile oocytes in aging females remains elusive. Leptin plays an important role in the maintenance of energy homeostasis, as both excessive or insufficient levels can affect the body weight and fertility of mice. Here, we report that leptin A deficiency affects growth and shortens reproductive lifespan by reducing fertility in medaka (Oryzias latipes). Targeted disruption of lepa (lepa-/-) females reduced their egg laying and fertility compared to normal 3-month-old females (lepa+/+ sexual maturity), with symptoms worsening progressively at the age of 6 months and beyond. Transcriptomic analysis showed that differentially expressed genes involved in metabolic and mitochondrial pathways were significantly altered in lepa-/- ovaries compared with the normal ovaries at over 6 months old. The expression levels of the autophagy-promoting genes ulk1a, atg7 and atg12 were significantly differentiated between normal and lepa-/- ovaries, which were further confirmed by quantitative polymerase chain reaction analysis, indicating abnormal autophagy activation and mitochondrial dysfunction in oocyte development lacking lepa. Transmission electron microscopy observations further confirmed these mitochondrial disorders in lepa-deficient oocytes. In summary, these research findings provide novel insights into how leptin influences female fertility through mitochondrial-mediated oocyte development.
RESUMO
Investigating the principles of fish fat deposition and conducting related research are current focal points in fish nutrition. This study explores the endocrine regulation of LEAP2 and GHSR1a in zebrafish by constructing mutantmodels andexamining the effects of the endocrine factors LEAP2 and its receptor GHSR1a on zebrafish growth, feeding, and liver fat deposition. Compared to the wild type (WT), the mutation of LEAP2 results in increased feeding and decreased swimming in zebrafish. The impact is more pronounced in adult female zebrafish, characterized by increased weight, length, width, and accumulation of lipid droplets in the liver.Incontrast, deficiency in GHSR1a significantly reduces the growth of male zebrafish and markedly decreases liver fat deposition.These research findings indicate the crucial roles of LEAP2 and GHSR1a in zebrafish feeding, growth, and intracellular fat metabolism. This study, for the first time, investigated the endocrine metabolic regulation functions of LEAP2 and GHSR1a in the model organism zebrafish, providing initial insights into their effects and potential mechanisms on zebrafish fat metabolism.
Assuntos
Peptídeos Catiônicos Antimicrobianos , Metabolismo dos Lipídeos , Receptores de Grelina , Peixe-Zebra , Animais , Feminino , Masculino , Sistemas CRISPR-Cas , Mutação , Receptores de Grelina/genética , Receptores de Grelina/metabolismo , Peixe-Zebra/genética , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo , Peptídeos Catiônicos Antimicrobianos/metabolismoRESUMO
Liver-expressed antimicrobial peptide 2 (LEAP2) is a blood-derived antimicrobial peptide expressed predominantly in the liver. Although LEAP2 has been reported to exert antimicrobial effects in various fish species, its antimicrobial mechanism is not entirely understood. Zebrafish is an intensively developing animal model for studying bacterial diseases. In this study, we used zebrafish to identify the role of LEAP2 in bacterial infection. We found that knockout of LEAP2 in zebrafish led to a higher bacterial burden and mortality. To further investigate the effect of LEAP2 mutation on the immune system, we conducted a comparative transcriptome analysis of zebrafish with a mutant of LEAP2. Based on gene ontologies (GO) enrichment, LEAP2 mutant zebrafish revealed that, compared to wild-type zebrafish, robust responses to bacteria, inflammatory factors, and disrupt immune homeostasis and induct hyperinflammation. Furthermore, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, six immune pathways were identified: Phagosome, NOD-like receptor, ferroptosis, Cytokine-cytokine receptor, Toll-like receptor, and FOXO signalling pathways. Interestingly, besides the liver, muscle, intestine, and eggs are also significantly enriched to the ferroptosis pathway, as revealed using quantitative polymerase chain reaction (qPCR), further confirmed that the effect of LEAP2 mutations on inflammatory factors and ferroptosis-related genes. Most importantly, this is the first report of the zebrafish LEAP2 mutant transcriptome obtained using high-throughput sequencing. Our study employed comparative transcriptome analysis to reveal the inflammatory response and ferroptosis-signalling pathway as a novel potential mechanism of LEAP2 antibacterial activity, laying the foundation for future studies of LEAP2 immune functions.
Assuntos
Aeromonas hydrophila , Peixe-Zebra , Animais , Aeromonas hydrophila/fisiologia , Perfilação da Expressão Gênica/veterinária , Transcriptoma , Antibacterianos , Citocinas/genéticaRESUMO
Liver-expressed antimicrobial peptide 2 (LEAP2) is a small peptide, which is consisted of signal peptide, pro-peptide and the bioactive mature peptide. Mature LEAP2 is an antibacterial peptide with four highly conserved cysteines forming two intramolecular disulfide bonds. Chionodraco hamatus, an Antarctic notothenioid fish that lives in the coldest water, has white blood unlike most fish of the world. In this study, the LEAP2 coding sequence was cloned from C. hamatus, including a 29 amino acids signal peptide and mature peptide of 46 amino acids. High levels of LEAP2 mRNA were detected in the skin and liver. Mature peptide was obtained by chemical synthesis in vitro, displayed selective antimicrobial activities against Escherichia coli, Aeromonas hydrophila, Staphylococcus aureus and Streptococcus agalactiae. Liver-expressed antimicrobial peptide 2 showed bactericidal activity by destroying the cell membrane integrity and robustly combined with bacterial genomic DNA. In addition, overexpression of the Tol-LEAP2-EGFP in zebrafish larva showed stronger antimicrobial activity in C. hamatus than in zebrafish, accompanied by lower bacterial load and expression of pro-inflammatory factors. This is the first demonstration of the antimicrobial activity of LEAP2 from C. hamatus, which is of useful value in improving resistance to pathogens.
Assuntos
Anti-Infecciosos , Doenças dos Peixes , Perciformes , Animais , Peixe-Zebra , Hepcidinas , Perciformes/genética , Peptídeos , Aminoácidos , Sinais Direcionadores de Proteínas , Antibacterianos/farmacologiaRESUMO
Ordinary cement is not environmentally friendly, has high cost and lacks superior performance. Many scholars use various admixtures to adjust the properties of cement slurry, but admixtures are usually not environmentally friendly, and it is difficult to ensure that the properties after deployment meet engineering requirements. In this study, a variety of admixtures were obtained using the environmental protection method, and the optimal mixing ratio was analyzed by combining the entropy weight method and the Taguchi grey relational analysis method. The developed cement slurry was compared with conventional slurry from both macroscopic and microscopic aspects. Aiming at the problem that previous scholars lacked the engineering feasibility verification of the developed slurry, this study combined the constitutive equation regression analysis method, discrete element numerical simulation and other methods to study various actual engineering conditions. The results show that the optimal mix ratio of silica fume cement slurry has good permeability characteristics under the conditions of different roughness, grouting pressure and confining pressure. At the same time, under different geological temperatures and different erosive liquid states, the cement slurry stone body shows good properties of reinforcement materials.