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1.
Int J Biol Macromol ; 275(Pt 2): 133251, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38945708

RESUMO

Bioactive hydrogels are currently receiving significant attention. In this study, silk fibroin tyramine-modified gelatin hydrogels (SF-TG) with varying degrees of tyramine root substitution were explored. The physicochemical property and biocompatibility of low degree of substitution tyramine-modified gelatin hydrogel (SF-LTG) and high degree of substitution tyramine-modified gelatin hydrogel (SF-HTG) were compared. The results showed that SF-LTG possessed better mechanical property and higher biocompatibility. Thus, SF-LTG was selected as a bioactive matrix and loaded with basic fibroblast growth factor (bFGF); subsequently, curcumin-coupled chitosan rods (CCCRs-EGF) enriched with epidermal growth factor (EGF) were added to obtain SF-LTG-bFGF@CCCRs-EGF hydrogels. The results showed that SF-LTG-bFGF@CCCRs-EGF retained the basic structural and mechanical properties of the SF-LTG matrix gel material and underwent multiple loading and orderly release with different activities while displaying antioxidant, anti-inflammatory, antimicrobial, and pro-cellular proliferation activities and orderly regulation of activity during wound healing. Therefore, the SF-LTG-bFGF@CCCRs-EGF hydrogel is of great value in healing complex wounds.


Assuntos
Quitosana , Fator de Crescimento Epidérmico , Fibroínas , Hidrogéis , Fibroínas/química , Hidrogéis/química , Hidrogéis/farmacologia , Animais , Fator de Crescimento Epidérmico/química , Quitosana/química , Curcumina/química , Curcumina/farmacologia , Gelatina/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Fator 2 de Crescimento de Fibroblastos/química , Antioxidantes/química , Antioxidantes/farmacologia , Camundongos , Cicatrização/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Liberação Controlada de Fármacos , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Humanos
2.
Anal Methods ; 16(8): 1185-1195, 2024 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-38305686

RESUMO

Silk is a symbol of ancient Chinese civilization that has made an indelible contribution to the development of world civilization. However, because ancient artifacts are often contaminated or degraded, it is difficult to detect the presence of silk therein, and the true origin of silk thus remains a mystery. Therefore, this work presents a flexible pressure immunosensor that was designed based on 3D polypyrrole (PPy) foams for the trace detection of silk fibroin at archaeological sites. Initially, silk fibroin (SF) was conjugated with antibody-functionalized copper oxide nanoparticles (CuO NPs) and carboxylated magnetic beads (MBs) to form a sandwich immune complex. Then, the sandwich immune complex was added to hydrogen peroxide (H2O2) by magnetic separation to catalyse the generation of oxygen (O2), which converted the antigen-antibody specific recognition signal to gas pressure. As the pressure within the device increases, the 3D PPy foam, as the sensing layer resistance was 150 Ω, undergoes extrusion and deformation. This deformation leads to alterations in the foam resistance. The flexible pressure immunosensor can sensitively monitor the change in electrical resistance in the system and quantitatively detect silk fibroin. With optimization, the flexible pressure immunosensor demonstrates a dynamic range of operation spanning from 10 ng mL-1 to 100 µg mL-1, exhibiting a remarkable detection limit of 10.58 ng mL-1 specifically for silk fibroin. Notably, this immunosensor surpasses enzyme-linked immunosorbent assay (ELISA) in terms of superior reproducibility, specificity, and accuracy. Therefore, this application provides a new method and technical support for silk detection.


Assuntos
Técnicas Biossensoriais , Fibroínas , Fibroínas/análise , Polímeros , Imunoensaio/métodos , Técnicas Biossensoriais/métodos , Peróxido de Hidrogênio , Complexo Antígeno-Anticorpo , Reprodutibilidade dos Testes , Pirróis , Seda
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