RESUMO
The antioxidant balance, photochemical activity of photosystem II (PSII), and photosynthetic pigment content, as well as the expression of genes involved in the light signalling of callus lines of Eutrema salsugineum plants (earlier Thellungiella salsuginea) under different spectral light compositions were studied. Growth of callus in red light (RL, maximum 660 nm), in contrast to blue light (BL, maximum 450 nm), resulted in a lower H2O2 content and thiobarbituric acid reactive substances (TBARS). The BL increased the activities of key antioxidant enzymes in comparison with the white light (WL) and RL and demonstrated the minimum level of PSII photochemical activity. The activities of catalase (CAT) and peroxidase (POD) had the highest values in BL, which, along with the increased H2O2 and TBARS content, indicate a higher level of oxidative stress in the cells. The expression levels of the main chloroplast protein genes of PSII (PSBA and PSBD), the NADPH-dependent oxidase gene of the plasma membrane (RbohD), the protochlorophyllide oxidoreductase genes (POR B, C) involved in the biosynthesis of chlorophyll, and the key photoreceptor signalling genes (CIB1, CRY2, PhyB, PhyA, and PIF3) were determined. Possible mechanisms of light quality effects on the physiological parameters of callus cells are discussed.
Assuntos
Antioxidantes/metabolismo , Brassicaceae/genética , Brassicaceae/metabolismo , Complexo de Proteína do Fotossistema II/metabolismo , Proteínas de Plantas/genética , Brassicaceae/citologia , Carotenoides/metabolismo , Sobrevivência Celular , Clorofila/metabolismo , Clorofila A , Enzimas/metabolismo , Regulação da Expressão Gênica de Plantas , Peróxido de Hidrogênio/metabolismo , Luz , Fotorreceptores de Plantas/genética , Fotorreceptores de Plantas/metabolismo , Complexo de Proteína do Fotossistema II/genética , Proteínas de Plantas/metabolismo , Transdução de Sinais/genética , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Técnicas de Cultura de TecidosRESUMO
Inhibitory effects of polycyclic aromatic hydrocarbons (PAHs) on plants were studied in pea leaves in order to elucidate the mechanisms of action of PAHs such as naphthalene (Naph) and phenanthrene (Phen) on activity of photosystem II (PSII). The changes in different Chl fluorescence parameters were calculated on the basis of Chl fluorescence induction curves. H2 O2 content was measured in leaf homogenates with the luminol-dependent chemiluminescence method. We demonstrated that following PAH treatment, total energy dissipation (DI0 /ABS) and amount of QB -non-reducing complexes of PSII significantly increased. Non-photochemical quenching (NPQ) also increased, when weak oxidative stress after PAH application developed. In leaves, a two-step increase in H2 O2 was found with time of incubation in the presence of PAHs, which may be associated with damage to the lipid bilayer of the plasma membrane and then violation of lipid bilayer membranes of cell organelles. A hypothesis for the mode of action of PAHs is provided that involves the role of ROS, membrane permeability and associated functional changes in PSII.