RESUMO
Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding (H-) proteins. Gel chromatography of the H+ portion yielded four main protein fractions of >150, 45, 30 and 20 kDa, while that of the H- portion resulted in the separation into three main protein fractions of >150, 30 and 20 kDa. HPLC analysis and N-terminal sequencing used to characterize the composition of the protein fractions obtained by gel chromatography revealed that all consisted of low (12-16 kDa) molecular weight components: the H+ fraction consisted of DQH sperm protein, AQN and AWN spermadhesins whereas the H- fraction consisted of PSPI and PSPII spermadhesins. The high molecular weight values of fractions obtained by gel chromatography thus suggest that the proteins are present in boar seminal plasma in the form of aggregates. Interactions of individual boar seminal plasma proteins and their aggregates present in the H+ and H- fractions with acid polysaccharides were estimated.
Assuntos
Heparina/metabolismo , Proteínas/metabolismo , Sêmen/química , Suínos/metabolismo , Animais , Líquidos Corporais/química , Sulfatos de Condroitina/metabolismo , Cromatografia de Afinidade , Cromatografia Líquida de Alta Pressão , Sulfato de Dextrana/metabolismo , Ácido Hialurônico/metabolismo , Substâncias Macromoleculares , Masculino , Peso Molecular , Polissacarídeos/metabolismo , Ligação Proteica , Proteínas/isolamento & purificação , Análise de Sequência de Proteína , Capacitação Espermática , Zona Pelúcida/metabolismoRESUMO
PIP: Since the insertion of an IUD may involve many complications, it is apparent that many women will be unable to work should they occur. Between 1966-1972, 3723 Dana IUDs were inserted in a clinic in Kosice, Czechoslovakia. There were 176 complications (4.7%) requiring hospitalization. The most frequent reasons for inability to work were injuries to the uterus, extrauterine pregnancies, and infections.^ieng