Nutritional and plasmatic antioxidant vitamins status of ultra endurance athletes.

OBJECTIVE: The "Marathon des Sables" (MDS) is a competition known to induce oxidative stress. Antioxidant vitamins prevent exercise-induced oxidative damages. The purpose of this study was to evaluate daily intake and plasma level of the main antioxidant vitamins (alpha-tocopherol, vitamin C, beta-carotene and retinol) in 19 male athletes who participated in this competition. METHODS: Data collected before the beginning of the competition included daily dietary intake using a 7-day food record and plasma biochemical measurements (alpha-tocopherol, vitamin C, beta-carotene and retinol). RESULTS: First, total energy intake was obviously lower than the energetic intake usually observed in well-trained endurance athletes. Second, antioxidant vitamins intake was also insufficient. Indeed, the intake was lower than the French Dietary Reference Intakes (DRI) for this population in 18 subjects for vitamin E and 6 subjects for vitamin C, beta-carotene and Retinol Equivalent. As a significant relationship was found between total energy intake and the intake of vitamin E (r = 0.73; p < 0.001) and vitamin C (r = 0.78; p < 0.001), the low total energy intake contributed partially to the insufficient antioxidant vitamins intake. The dietary questionnaire analysis also revealed a low intake of vegetable oils, fruits and vegetables. However, plasma concentrations of these antioxidant vitamins were similar to the literature data observed in athletes. CONCLUSION: This study evidenced obvious insufficient energy intake in ultra endurance athletes associated with a low antioxidant vitamin intake.

Multivitamin-mineral supplementation prevents lipid peroxidation during "the Marathon des Sables".

OBJECTIVE: We investigated the effect of a moderate mutivitamin and mineral supplementation containing mainly vitamin C (150.0 mg.day(-1)), vitamin E (24.0 mg.day(-1)) and beta-carotene (4.8 mg.day(-1)) prior to and during an extreme running competition -the Marathon des Sables (MDS)- that consisted of six long races in the desert. METHODS: Seventeen athletes participated in our double blind, placebo-controlled study. Blood samples were collected prior to the supplementation i.e. three weeks before the competition (D-21), two days prior to the MDS (D-2), after the third race (D3) and at the end of the competition (D7). Erythrocyte antioxidant enzyme activity (glutathione peroxidase (GPx), superoxide dismutase (SOD)), erythrocyte glutathione level (GSH), plasma non-enzymatic antioxidant status (uric acid, vitamin C, alpha-tocopherol, retinol, beta-carotene), markers of plasma lipid peroxidation (thiobarbituric reactive substances (TBARS)), reactive carbonyl derivatives (RCD) and membrane damage (creatine kinase and lactate dehydrogenase activities) were measured. RESULTS: In both groups, GSH levels, uric acid levels and membrane damage significantly increased during the competition while SOD activity significantly decreased. In Supplemented group, plasma alpha-tocopherol, beta-carotene and retinol levels significantly increased after three weeks of supplementing. In contrast to Placebo group, alpha-tocopherol, vitamin C and retinol levels were significantly affected by the competition in Supplemented group. Moreover, no increase in TBARS was observed in Supplemented group during the competition, whereas TBARS significantly increased at D3 in the placebo group. CONCLUSION: The moderate multivitamin-mineral supplementation prevented the transient increase in TBARS levels during this extreme competition.

Extreme running competition decreases blood antioxidant defense capacity.

OBJECTIVE: We tested whether an extreme running competition ("Marathon of Sands") might alter the blood's enzymatic and non-enzymatic antioxidant status in 6 well-trained athletes. METHODS: The Marathon of Sands is a competition consisting of six long duration races in the desert in which the athletes carry their own food. Blood samples were collected from an antecubital vein while the athletes were at rest before the competition and then again 72 hours after. Erythrocyte antioxidant enzyme activity (glutathione peroxidase, superoxide dismutase), erythrocyte glutathione level, plasma non-enzymatic status (vitamin C, alpha-tocopherol, retinol, beta-carotene and carotenoids) and plasma lipid peroxidation marker (TBARS) were measured. RESULTS: The Marathon of Sands induced a significant alteration of the blood antioxidant defense capacity. Indeed, 72 hours after the race, significant decreases were recorded in erythrocyte superoxide dismutase activity and in plasma concentrations of retinol, beta-carotene and other carotenoids. These changes were associated with a concomitant increase in erythrocyte glutathione and in plasma TBARS levels. CONCLUSION: This study indicated that such extreme competition induced an imbalance between oxidant and antioxidant protection.

Physical fitness and plasma non-enzymatic antioxidant status at rest and after a wingate test.

We tested seven physical education students whether 30-s sprint anaerobic exercise (Wingate test) would result in oxidative stress (evaluated by lipid radical levels) sufficient to alter plasma non-enzymatic antioxidant status (plasma uric acid, ascorbic acid, alpha-tocopherol, beta-carotene). This study demonstrates that 1) Wingate test increases plasma uric and ascorbic acid concentrations (p <.05), and decreases plasma alpha-tocopherol and beta-carotene levels (p <.05); 2) lipid radical levels at rest and sprint performance are negatively correlated with resting plasma uric acid and alpha-tocopherol concentrations (p <.05). In conclusion, this study 1) demonstrates that a 30-s sprint anaerobic exercise is associated with acute changes in plasma non-enzymatic antioxidant status, 2) indicates that the subjects with largest leg peak power are those who exhibit the lowest plasma antioxidant status at rest (uric acid and alpha-tocopherol), 3) and suggests that antioxidant intake by maintaining plasma antioxidant concentration at rest in the normal range might protect athletes against oxidative stress induced by exercise.