Preliminary findings on the gastrointestinal parasites of the brown bear (Ursus arctos) in the Cantabrian mountains, Spain.

No study is currently available on the parasitofauna of the population of brown bears (Ursus arctos) inhabiting the Cantabrian Mountains in Spain. The aim of the present study was to obtain novel information on diversity and prevalence of gastrointestinal parasites in these individuals. During August 2016 and from May to July 2017, 14 fecal samples were collected from the western Cantabrian bear subpopulation, in the Somiedo Natural Park, in the Spanish province of Asturias. The prevalence of parasites detected was 71% and two genera were identified: Dicrocoelium sp. and Trichuris sp. Since the impact that pathogens such as endoparasites can have on the health of bears, together with other stressors, is still poorly understood, research efforts that include disease surveillance are critical to the successful protection of this emblematic species. Our preliminary findings require further investigations, with a wider sampling effort, and bring awareness for the need of carrying further studies on this area as a part of a proactive species management plan.

Nuclear and mitochondrial marker sequences reveal close relationship between Coronocyclus coronatus and a potential Cylicostephanus calicatus cryptic species complex.

The Cyathostominae (Nematoda, Strongyloidea) parasitising equines represent a diverse group currently including 50 species. However, their taxonomy has been repeatedly revised and occasionally the presence of cryptic genospecies was suggested. Moreover, molecular- and morphology-based phylogenetic analyses give divergent results. For instance, molecular data have suggested close relationship between Coronocyclus coronatus and Cylicostephanus calicatus, although morphology-based taxonomy places them in different genera. Here, nuclear (internal transcribed spacer 2, ITS-2) and mitochondrial (cytochrome oxidase I, COI) sequences were obtained from the same individual, morphologically identified worms. In both morphospecies, two ITS-2 sequences types were observed: In Cor. coronatus, a small PCR product of 278 bp (nuclear haplotype group nHGBco) was always present but often in combination with a larger 369-370 bp fragment (nHGAco). In Cys. calicatus, either a large 370 bp product (nHGAca) or a short 281 bp amplicon (nHGBca) were found, but never both. Sequence identity between morphospecies was up to 100%. The smaller differed from the larger fragments by deletion of the region 110-198 in Cor. coronatus and 112-203 in Cys. calicatus. In COI, three and five mitochondrial haplotype groups (HGs), mtHG1co-mtHG3co and mtHG1ca-mtHG5ca were identified for Cor. coronatus and Cys. calicatus, respectively. In Cor. coronatus, there was no particular association of mtHG with nuclear genotypes (only nHGBco vs. both nHGBco plus nHGAco). In Cys. calicatus the nHGAca was always associated with the mtHG1ca, mtHG2ca or mtHG5ca whereas nHGBca was exclusively associated with mtHG3ca or mtHG4ca. Despite up to 100% identity in the nHGs, no mixing of mtHGs was observed between both species. Clear separation of certain nHGs with particular mtHGs in Cys. calicatus, despite the fact that the same host individuals were infected with both groups simultaneously, suggests presence of two non-interbreeding genospecies within Cys. calicatus, which needs further confirmation using additional samples from diverse geographical origins.

Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania.

Resistance to anthelmintic medication of equid strongyles is a worldwide phenomenon and for this reason systematic investigations of resistant parasite populations are necessary. The purpose of the present study was to investigate the presence and distribution of equid strongyles resistant to the anthelmintics used in Romania, as well as the pre-treatment and post-treatment prevalence of species of strongylid nematodes. The Faecal Egg Count Reduction Test was performed between 2010 and 2013 on a total number of 588 horses and 23 donkeys from 26 locations (subgroups). Animals of the first group (I) consisting of subgroups no. 1-11 were treated with Albendazole (ABZ), those of the second group (II) consisting of subgroups no. 12-23 with Fenbendazole (FBZ), while Ivermectin (IVM) was used on animals of the third group (III) consisting of subgroups no. 24-26. Resistant strongyles have been found in 82% (average lower limit of the 95% confidence interval, LCL95%, was 65) of the total equids from the group treated with ABZ. In the group of horses treated with FBZ, resistant strongyles were identified in 75% of the subgroups (LCL95% = 44). No resistant strongyles have been identified in IVM-treated horse groups (LCL95% = 98). The pre-treatment prevalence of the species of the Strongylinae Müller, 1780 was 22%, whereas that with nematodes of the subfamily Cyathostominae Molin, 1861 78%. Post-treatment reduction of strongyline nematodes was observed (5%), which demonstrates the sensitivity of large strongyles to common anthelmintics. The post-treatment prevalence of cyathostomes was of 95%, which proves their resistance, especially to ABZ- and FBZ-based anthelmintics.

Survey of infectious and parasitic diseases in stray cats at the Lisbon Metropolitan Area, Portugal.

A survey of infectious and parasitic diseases of stray cats was carried out using biological samples collected from animals captured during a catch-neuter-release programme in four counties of the Lisbon Metropolitan Area. The main objective was to investigate the potential threat of stray cats for animal and public health. Samples of blood, stool, hair and auricular swabs were collected from 231 cats in 27 colonies. Anti-Toxoplasma gondii antibodies were detected in 47/194 samples (24.2%); anti-Leishmania infantum antibodies in 1/180 cats (0.6%); intestinal parasites in 23/74 samples (Toxocara cati, Isospora felis, Ancylostoma tubaeforme, Dipylidium caninum, Uncinaria stenocephala, Toxascaris leonina) and Otodectes cynotis in 4/182 cats (2.2%); dermatophyte fungi were isolated in 40/136 samples (29.4%); feline immunodeficiency virus antibodies were detected in 23/226 samples (10.2%); feline leukaemia virus antigen in 14/198 samples (7.1%); and feline coronavirus RNA in 9/127 samples (7.1%). Our results revealed that zoonotic agents, namely dermatophyte fungi and Toxocara cati were present in stray cat colonies in the investigated counties. Overall the low frequency of major pathogens suggests a balanced relationship between host and agents.