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1.
Vet J ; 216: 207-9, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27687954

RESUMO

Senecavirus A (SV-A) may cause vesicular disease and neonatal mortality in pigs, and was first detected in Brazil in 2015. Samples including tissues and serum from pigs with suspected vesicular diseases were collected from January to August in 2015 from farms in the states of Minas Gerais, Santa Catarina, Goiás and Rio Grande do Sul, Brazil, and tested for the presence of SV-A by reverse transcriptase PCR. All samples were negative for foot and mouth disease virus, as well as 13 other infectious agents associated with vesicular diseases in pigs. SV-A was detected by PCR in 65/265 (24.5%) specimens. A 530 base pair fragment sequenced from the VP1 protein coding region indicated a high genetic distance from SV-A in other countries, but a common origin among the Brazilian isolates.


Assuntos
Infecções por Picornaviridae/veterinária , Picornaviridae/fisiologia , Doenças dos Suínos/epidemiologia , Proteínas Virais de Fusão/genética , Sequência de Aminoácidos , Animais , Brasil/epidemiologia , Filogenia , Picornaviridae/genética , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Análise de Sequência de RNA/veterinária , Suínos , Doenças dos Suínos/virologia , Doença Vesicular Suína/virologia
2.
J Vet Diagn Invest ; 24(2): 355-8, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22379052

RESUMO

Vaccinia virus (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were -1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine.


Assuntos
Anticorpos Antivirais/sangue , Doenças dos Bovinos/virologia , Técnicas Imunoenzimáticas/veterinária , Vaccinia virus/isolamento & purificação , Vacínia/veterinária , Animais , Brasil , Bovinos , Feminino , Técnicas Imunoenzimáticas/métodos , Testes de Neutralização/veterinária , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Vacínia/sangue , Vacínia/virologia
3.
J Clin Virol ; 48(1): 69-72, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20207192

RESUMO

BACKGROUND: Occupational exanthematic diseases represent an important cause of public health impact and economical losses. Among the viral exanthematic diseases, two caused by poxviruses are noteworthy: the bovine vaccinia (BV), caused by the Vaccinia virus (VACV); and the milker's nodule, in which the agent is the Pseudocowpox virus (PCPV). Both agents are zoonotic and have been associated with several cases of bovine infection. In Brazilian rural areas BV has been highly prevalent, particularly in milk herds. Farmers, milkers and their close contacts developed lesions on the hands, forearms, legs and face accompanied by several systemic symptoms. Although VACV and PCPV present with similar epidemiological and transmission patterns, no VACV and PCPV co-infection cases have to date been described. OBJECTIVES: To describe the first case of zoonotic VACV and PCVP co-infection, based on serological and molecular methods. STUDY DESIGN AND RESULTS: In this work we report a case of a Brazilian rural worker who presented with a large severely ulcerated-pustule skin lesion, associated with fever, headache, malaise, myalgia and axillary, inguinal and cervical limphadenopathy. The worker declared occupational contact with cattle that had notable injuries on their teats. Human and bovine clinical samples were collected and submitted to serological and molecular tests. PCR and phylogenetic analysis revealed the presence of VACV DNA and PCPV DNA in the patient's lesion. Serological tests indicated anti-VACV neutralizing antibodies and molecular assays showed the presence of VACV and PCPV DNA in the patient sera. VACV and PCPV also were detected in dairy cattle. CONCLUSION: Together, these results indicate a case of zoonotic VACV/PCPV co-infection. Epidemiological surveillance and appropriate medical treatment are essential for the control of both diseases, especially in the most severe cases, as described in the present study.


Assuntos
Infecções por Poxviridae/virologia , Vírus da Pseudovaríola das Vacas/genética , Vaccinia virus/genética , Vacínia/virologia , Zoonoses/virologia , Animais , Brasil , Bovinos , Dedos/patologia , Dedos/virologia , Humanos , Masculino , Filogenia , Infecções por Poxviridae/diagnóstico , Pele/patologia , Pele/virologia , Adulto Jovem
4.
Foodborne Pathog Dis ; 6(9): 1141-6, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19888798

RESUMO

The vaccinia virus (VACV), which causes exanthemous lesions in dairy cattle and humans, has been associated with several bovine vaccinia outbreaks in Brazil. Currently, no data are available about the safety of milk produced in VACV-affected areas. In this study, 47 milk samples were collected during bovine vaccinia outbreaks and submitted to viral isolation, DNA detection, and nucleotide sequencing of the conserved tk gene. The appearance of characteristic white pocks on the chorioallantoic membranes of chicken eggs, in association with viral cytopathic effects in chicken embryo fibroblasts and phylogenetic data, strongly suggest milk contamination by VACV. This is the first report of VACV detection in and isolation from milk.


Assuntos
Doenças dos Bovinos/epidemiologia , Surtos de Doenças/veterinária , Leite/virologia , Vaccinia virus/isolamento & purificação , Vacínia/veterinária , Animais , Bioensaio , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/transmissão , Doenças dos Bovinos/virologia , Embrião de Galinha , DNA Viral/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular , Peptídeos/genética , Peptídeos/metabolismo , Filogenia , Reação em Cadeia da Polimerase , Timidina Quinase/genética , Timidina Quinase/metabolismo , Vacínia/epidemiologia , Vacínia/transmissão , Vacínia/virologia , Vaccinia virus/metabolismo , Vírion/isolamento & purificação
5.
Emerg Infect Dis ; 11(12): 1935-8, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16485483

RESUMO

Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.


Assuntos
Filogenia , Vaccinia virus/classificação , Vaccinia virus/isolamento & purificação , Vacínia/epidemiologia , Vacínia/virologia , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/patologia , Doenças dos Bovinos/virologia , Surtos de Doenças , Humanos , Exposição Ocupacional , Vacínia/veterinária , Vaccinia virus/genética , Vaccinia virus/patogenicidade , Zoonoses
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