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1.
Mikrobiol Zh (1978) ; 51(5): 77-81, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2622400

RESUMO

Representatives of the normal microflora from genus Aerococcus, in particular strain Aerococcus viridans 167 isolated from breast milk are studied for their effect on biological properties of Staphylococcus aureus in vitro and in vivo. It is established that the number of viable cells of the staphylococcus cultivated in the presence of antagonists in the beef-extract agar decreases progressively with each following passage, the population dying after the seventh-eight passage. Electronograms fix deep changes in the cell ultrastructure. A degree of changes in biological properties depends on the duration of the antagonist action. The results obtained reveal one of the mechanisms of the antagonistic action of aerococci-antagonists producing hydrogen peroxide.


Assuntos
Vacinas Bacterianas/uso terapêutico , Produtos Biológicos/uso terapêutico , Staphylococcus aureus/fisiologia , Streptococcaceae/fisiologia , Animais , Antibiose/fisiologia , Contagem de Colônia Microbiana , Humanos , Camundongos , Leite Humano/microbiologia , Inoculações Seriadas , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/terapia , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/ultraestrutura , Streptococcaceae/isolamento & purificação , Streptococcaceae/ultraestrutura
2.
Lab Delo ; (10): 64-5, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2481080

RESUMO

The rates of Yersinia isolation from 156 samples of pathologic material cultivated by two methods, the routine one, with the selection of Yersinia from a microbial association by prolonged cooling of the examined material in slightly alkaline buffer and multiple inoculations into Endo's medium, and the technique suggested by the authors--a rapid method based on the treatment of the biologic material with a concentrated alkaline-salt mixture at 4 degrees C for 2 days and a single inoculation into inhibiting selective medium. Use of the rapid method has increased the isolation rate of Yersinia 4-fold, cut down the time of the investigation 2-3-fold, reduced the cost and simplified the technique of analysis.


Assuntos
Compostos de Potássio , Yersiniose/microbiologia , Yersinia enterocolitica/isolamento & purificação , Doença Aguda , Meios de Cultura , Humanos , Sulfato de Magnésio/farmacologia , Fosfatos/farmacologia , Potássio/farmacologia , Cloreto de Sódio/farmacologia , Yersiniose/diagnóstico , Yersinia enterocolitica/efeitos dos fármacos , Yersinia enterocolitica/crescimento & desenvolvimento
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