Bethanechol-induced restricted stimulation of pancreatic juice secretion in mice.

Flow and enzymatic output from mouse pancreas were studied "in vivo" under bethanechol stimulation. A biphasic dose response curve was found in both, enzymatic output and juice flow, implicating that exocrine pancreatic juice secretion was also involved in restricted stimulation phenomenon.

Serum isoamylase activities in cystic fibrosis patients, determined by an inhibitory assay.

Serum isoamylase activities were determined in patients with either cystic fibrosis (CF) or celiac disease and in age-matched healthy children by means of an inhibitory assay suitable for routine application. The percentage pancreatic isoamylase (PIA) activity was significantly decreased in CF patients when compared with that of patients with celiac disease. Cystic fibrosis patients with steatorrhea had lower absolute values of PIA activity and higher values of salivary isoenzyme activity than control individuals. There was no overlapping between values obtained from CF patients and either celiac patients or healthy individuals. These results suggest that determination of PIA activity may be a useful adjunct test for the diagnosis of CF in patients with steatorrhea.

[Calcium secretion "in vivo" by mouse exocrine pancreas. Relationship with enzymatic output]. / Estudio in vivo de la secreción de calcio por el pancreas exócrino del ratón. Relación con la secreción enzimática.

Calcium secretion and chymotrypsin (CHT) outputs in pancreatic juice from mice were studied "in vivo". Pancreatic juice was collected after stimulation with secretin 32 mU/g. Secretin 32 mU/g plus cholecystokinin 16 mU/g (CCK) or secretin 32 mU/g plus bethanechol 0.2 ug/g. Calcium and CHT outputs were lower in mice treated with secretin alone than in those treated either with secretin plus CCK or with secretin plus bethanechol. Bethanechol or CCK addition to secretin produced a positive correlation between both parameters. Extrapolation of the regression line correlating calcium and CHT outputs indicates that at zero CHT output pancreatic juice contains calcium. These results suggest that calcium in mice pancreatic juice is produced by two different sources.

Tratamiento de la tuberculosis pulmonar bacilífera del adulto: evaluación de una asociación isoniacida-rifampicida-pirazinamida a dosis fijas en un comprimido / Treatment of the bacillary pulmonary tuberculosis of adults: evaluation of an association of isoniazid-rifampicin-pyrazinamide at fixed doses in a tablet

Se evalúa la efectividad y tolerancia de la asociación de isoniacida, rifampicina y pirazinamida a dosis fijas en comprimidos, p ara el tratamiento de la tuberculosis pulmonar bacilífera en su fase inicial. Los resultados fueron similares a los del empleo de las tres drogas por separado, con la ventaja de su fácil y cómoda administración

An experimental model to perform dynamic studies of exocrine pancreatic secretion in mice.

An experimental model to perform dynamic studies of exocrine pancreatic secretion from mice has been developed. It consists of a microsurgical procedure in anesthetized mice using a stereoscopic microscope. The bile-pancreatic common duct was individualized, isolated and cannulated. A specially calibrated capillary tube was placed in the free end of the cannula and put on a millimetrical rule. Readings of pure pancreatic juice flow rate were performed minute by minute to obtain a flow rate curve. The procedure allowed the discrimination among increasing betanechol doses of 0.1, 0.2, and 0.4 microgram/g body weight.

Development of pancreatic hydrolases in inbred mice.

Pancreatic content of lipase, amylase, trypsinogen, chymotrypsinogen and protein was studied in suckling and weaned mice at different ages. Protein content in pancreatic tissue was low at 15 days when compared with 30, 45 and 60 days of age. Specific activities of lipase and chymotrypsinogen were higher at 15 days of age, but chymotrypsinogen was not different at the studied ages post weaning. Amylase exhibited the highest value at 15 days and a marked depression at 30 days, and trypsinogen specific activity was not different between the studied groups. The present study demonstrates an age dependent enzyme pattern in mouse pancreas and reflects an active biosynthetic mechanism in suckling mice.

An experimental model to study bile and exocrine pancreatic secretion from mice.

An in vivo experimental model to obtain exocrine pancreatic secretion and bile from mice has been developed. It consists of a microsurgical procedure in anesthetized mice. The bile-pancreatic common duct and the duodenum were individualized, isolated, and cannulated using a stereoscopic microscope to obtain pure pancreatic juice, pure bile, bile-pancreatic juice, and duodenal contents. Intravenous injection of secretin as a pancreatic secretion stimulant was used with this experimental model. Fluid color an enzymatic activity were used as indicators of the fluid purity. In order to evaluate the overall procedure, mortality under surgery and volumes of samples obtained (expressed as microliters/30 minutes) before and after administration of secretin was measured. Results from a total of 524 mice of the BALB/c and DBA/2J-cri inbred strains were evaluated.