RESUMO
Fish samples initially collected by local veterinarians on the common and koi carp farms in Poland between 2013 and 2015 as part of a KHV surveillance programme, when the water temperature was between 16 and 26 °C, and were also tested for CEV by qPCR. A partial 478 nucleotide fragment of the 4a gene was subsequently generated from 17 qPCR-positive common carp Cyprinus carpio samples from 36 farm sites tested during the period. Sequence alignments and analysis revealed the presence of CEV in Poland both in common carp as well as in koi carp farms, and phylogenetic analysis assigned the Polish CEV sequences into three distinct genogroups. A lineage which includes the original sequences obtained from koi carp in Japan (genogroup II) included sequences from both koi carp and common carp, and the second lineage (genogroup I) contained sequences from common carp only. A third lineage (genogroup III) which was more closely related to the genogroup II also consisted of sequences from common carp only. The latter represents a lineage of CEV not previously described in the literature.
Assuntos
Carpas , Doenças dos Peixes/epidemiologia , Infecções por Poxviridae/veterinária , Poxviridae/fisiologia , Animais , Doenças dos Peixes/virologia , Filogenia , Polônia/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Poxviridae/genética , Infecções por Poxviridae/epidemiologia , Infecções por Poxviridae/virologia , Análise de Sequência de DNA/veterinária , Proteínas Virais/genéticaAssuntos
Carpas , Doenças dos Peixes/genética , Genes MHC Classe I , Infecções por Herpesviridae/veterinária , Herpesviridae/fisiologia , Animais , Regulação para Baixo , Doenças dos Peixes/virologia , Infecções por Herpesviridae/genética , Infecções por Herpesviridae/virologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismoAssuntos
Carpas , Doenças dos Peixes/epidemiologia , Variação Genética , Infecções por Rhabdoviridae/veterinária , Rhabdoviridae/genética , Animais , Doenças dos Peixes/virologia , Filogenia , Polônia/epidemiologia , Rhabdoviridae/fisiologia , Infecções por Rhabdoviridae/epidemiologia , Infecções por Rhabdoviridae/virologia , Análise de Sequência de DNA/veterináriaRESUMO
A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance Japan, China and Korea. The full-length sequence of the phosphoprotein gene (P) demonstrated a higher identity of the present isolate with HIRRV from China compared with the Korean isolate. An identical viral sequence was also found in brown trout, Salmo trutta trutta L., affected by mortalities in a second farm in the same region, after a likely contamination from the grayling farm. To our knowledge, this is the first report of HIRRV in Europe, and in two hosts from fresh water that have not been described before as susceptible species.