Polarized spectroscopy and SESAM mode-locking of Tm,Ho:CALGO.

A Tm,Ho:CALGO laser passively mode-locked by a GaSb-based SESAM generated pulses as short as 52 fs at a central wavelength of 2015 nm with a broad spectral bandwidth of 82 nm (full width at half maximum) owing to the combined gain profiles of both dopants for σ-polarized light. The average output power reached 376 mW at a repetition rate of 85.65 MHz. In the continuous-wave regime, the laser was power scaled up to 1.01 W at 2080.6 nm with a slope efficiency of 32.0%, a laser threshold of 155 mW and π-polarized emission. Polarized spectroscopic properties of Ho3+ ions in singly doped and codoped CALGO crystals were revisited to explain the observed laser performance.

Fractal dimension and directional analysis of elastic and collagen fiber arrangement in unsectioned arterial tissues affected by atherosclerosis and aging.

Structural proteins like collagen and elastin are major constituents of the extracellular matrix (ECM). ECM degradation and remodeling in diseases significantly impact the microorganization of these structural proteins. Therefore, tracking the changes of collagen and elastin fiber morphological features within ECM impacted by disease progression could provide valuable insight into pathological processes such as tissue fibrosis and atherosclerosis. Benefiting from its intrinsic high-resolution imaging power and superior biochemical specificity, nonlinear optical microscopy (NLOM) is capable of providing information critical to the understanding of ECM remodeling. In this study, alterations of structural fibrillar proteins such as collagen and elastin in arteries excised from atherosclerotic rabbits were assessed by the combination of NLOM images and textural analysis methods such as fractal dimension (FD) and directional analysis (DA). FD and DA were tested for their performance in tracking the changes of extracellular elastin and fibrillar collagen remodeling resulting from atherosclerosis progression/aging. Although other methods of image analysis to study the organization of elastin and collagen structures have been reported, the simplified calculations of FD and DA presented in this work prove that they are viable strategies for extracting and analyzing fiber-related morphology from disease-impacted tissues. Furthermore, this study also demonstrates the potential utility of FD and DA in studying ECM remodeling caused by other pathological processes such as respiratory diseases, several skin conditions, or even cancer. NEW & NOTEWORTHY Textural analyses such as fractal dimension (FD) and directional analysis (DA) are straightforward and computationally viable strategies to extract fiber-related morphological data from optical images. Therefore, objective, quantitative, and automated characterization of protein fiber morphology in extracellular matrix can be realized by using these methods in combination with digital imaging techniques such as nonlinear optical microscopy (NLOM), a highly effective visualization tool for fibrillar collagen and elastic network. Combining FD and DA with NLOM is an innovative approach to track alterations of structural fibrillar proteins. The results illustrated in this study not only prove the effectiveness of FD and DA methods in extracellular protein characterization but also demonstrate their potential value in clinical and basic biomedical research where protein microstructure characterization is critical.

Diode-pumped 45 fs Yb:CALGO laser oscillator with 1.7 MW of peak power.

A high-power sub-50 fs diode-pumped Yb:CALGO laser oscillator is demonstrated. The peak power achieved for 45 fs pulses directly from the oscillator was 1.7 MW. To the best of our knowledge, this is the highest peak power in the sub-50 fs pulse regime that has ever been produced directly from any diode-pumped Yb-ion-doped laser oscillator to date. The shortest generated pulses were 38 fs long with 187 kW of peak power.

Broadband and efficient adiabatic three-wave-mixing in a temperature-controlled bulk crystal.

Nonlinear interactions are commonly used to access to wavelengths not covered by standard laser systems. In particular, optical parametric amplification (OPA) is a powerful technique to produce broadly tunable light. However, common implementations of OPA suffer from a well-known trade-off, either achieving high efficiency for narrow spectra or inefficient conversion over a broad bandwidth. This shortcoming can be addressed using adiabatic processes. Here, we demonstrate a novel technique towards this direction, based on a temperature-controlled phase mismatch between the interacting waves. Using this approach, we demonstrate, by tailoring the temperature profile, an increase in conversion efficiency by 21%, reaching a maximum of 57%, while simultaneously expanding the bandwidth to over 300 nm. Our technique can readily enhance the performances of current OPA systems.

Femtosecond Alexandrite laser passively mode-locked by an InP/InGaP quantum-dot saturable absorber.

An Alexandrite laser passively mode-locked using an InP/InGaP quantum-dot semiconductor saturable absorber mirror (QD-SESAM) was demonstrated. The laser was pumped at 532 nm and generated pulses as short as 380 fs at 775 nm with an average output power of 295 mW. To the best of our knowledge, this is the first report on a passively mode-locked femtosecond Alexandrite laser using a SESAM in general and a QD-SESAM in particular.

High-power diode-pumped Kerr-lens mode-locked bulk Yb:KGW laser.

A high-power (>1 W) pure Kerr-lens mode-locked bulk Yb:KGW laser with multimode fiber-coupled diode pumping was demonstrated. The laser delivered 240 fs pulses with 2.3 W of average output power at 86.8 MHz, corresponding to 97 kW of peak power and 26 nJ of pulse energy. The shortest generated pulse duration was 120 fs with 1.2 W of output power. The self-starting regime was also observed with a background continuous-wave component. The laser exhibited more than an order of magnitude higher output power when compared to the previously reported bulk Yb-ion-doped lasers with multimode laser diode pumping systems.

High peak power ultrafast Yb:CaF2 oscillator pumped by a single-mode fiber-coupled laser diode.

We demonstrate a high peak power mode-locked Yb:CaF2 oscillator pumped by a single-mode laser diode. The laser operated in hybrid Kerr-lens and SESAM mode-locked regime. Its performance was optimized by varying the output coupler ratio. Pulses as short as 65 fs were generated with 0.4% transmission. Employing 5% output coupler enabled generation of 77 fs pulses with 46 kW of peak power (262 mW of average output power). We believe that such high peak powers can open a way to practical applications of single-mode diode-pumped ultrafast ytterbium lasers.

Discretely selectable multiwavelength operation of a semiconductor saturable absorber mirror mode-locked Nd:YVO4 laser.

We report on a semiconductor saturable absorber mirror mode-locked Nd:YVO4 laser operating at discretely selectable 1064.0, 1073.0, 1085.1 nm wavelengths with the average output powers of more than 2.2 W at each of the three wavelengths. The optical-to-optical efficiencies and the slope efficiencies were larger than 19% and 29% for each of the three wavelengths, and the pulse durations at the highest output powers were 13.1, 10.3, and 8.4 ps, respectively.

Microchip Yb:CaLnAlO4 lasers with up to 91% slope efficiency.

Multi-watt continuous-wave (CW) operation of tetragonal rare-earth calcium aluminate Yb:CaLnAlO4(Ln=Gd,Y)) crystals in plano-plano microchip lasers was demonstrated with an almost quantum-defect-limited slope efficiency. Pumped at 978 nm by an InGaAs laser diode, a 3.4 mm long 8 at. % Yb:CaGdAlO4 laser generated 7.79 W at 1057-1065 nm with a slope efficiency of η=84% (with respect to the absorbed pump power). An even higher η=91% was achieved with a 2.5 mm long 3 at. % Yb:CaYAlO4 laser, from which 5.06 W were extracted at 1048-1056 nm. Both lasers produced linearly polarized output (σ-polarization) with an almost circular diffraction-limited beam (Mx,y2<1.1). The output performance of the developed lasers was modeled, yielding an internal loss coefficient as low as 0.004-0.007 cm-1. In addition, their spectroscopic properties were revisited.

Sellmeier equations, group velocity dispersion, and thermo-optic dispersion formulas for CaLnAlO4 (Ln = Y, Gd) laser host crystals.

We studied the refractive index and dispersive properties of the tetragonal rare-earth calcium aluminates, CaLnAlO4 (Ln=Gd or Y). Sellmeier equations were derived for the spectral range of 0.35-2.1 µm. The group velocity dispersion (GVD) in CaGdAlO4 is positive at ∼1 µm, 95 fs2/mm and negative at ∼2 µm, -40 fs2/mm. The GVD values for CaYAlO4 are similar. In addition, thermo-optic coefficients, dn/dT, and thermal coefficients of the optical path were determined for CaYAlO4. dn/dT is negative at ∼1 µm, dno/dT=-7.8, and dne/dT=-8.7×10-6 K-1. Thermo-optic dispersion formulas were constructed. The obtained data are of key importance to the design of high-power mode-locked oscillators at ∼1 and ∼2 µm based on such laser hosts.

Polarization anisotropy of thermal lens in Yb:KY(WO4)2 laser crystal under high-power diode pumping.

Thermal lensing was studied in an Ng-cut monoclinic Yb:KY(WO4)2 (Yb:KYW) laser crystal under high-power diode pumping at ∼980 nm, for the two principal light polarizations, E||Nm and E||Np. For both polarizations, the thermal lens (TL) was positive. It was found that operation of an Yb:KYW laser with the E||Np polarization corresponds to a weaker TL that results in a better quality of the laser beam and superior power scaling capabilities. The sensitivity factors of the TL in this case were M=0.37 and 0.57 m-1/W for the mg and pg principal meridional planes, respectively, and the astigmatism degree was as low as S/M=35% (for a pump beam radius of wp=175 µm). The photoelastic terms, fractional heat loading, and generalized thermo-optic coefficients were evaluated.

Diode-pumped Nd:YVO4 laser with discrete multi-wavelength tunability and high efficiency.

Selectable and discretely tunable multi-wavelength diode-pumped Nd:YVO4 laser operating at 1064.0, 1073.1, and 1085.2 nm was demonstrated using a single intracavity birefringent plate. Under 11.2 W of absorbed pump power, the maximum output power of more than 3.8 W for any of the three wavelengths was achieved with slope and optical-to-optical efficiencies of more than 45% and 35%, respectively.

Multiwatt continuous wave Nd:KGW laser with hot-band diode pumping.

We have demonstrated what we believe is the first continuous wave neodymium-doped potassium gadolinium tungstate crystal (Nd:KGW) laser with hot-band diode pumping at ∼910 nm. This pumping wavelength reduced the quantum defect by >46% as compared to conventional ∼810 nm pumping and resulted in significantly lower thermal lensing. The laser produced 2.9 W of average output power at 1067 nm in a diffraction-limited beam for an absorbed pump power of 8.3 W. The slope efficiency and optical-to-optical efficiency were found to be 43% and 35%, respectively. Significant reduction of quantum defect offered by this pumping wavelength and availability of suitable high-power laser diodes opens up an attractive way to further power and efficiency scaling of Nd:KGW lasers.

High efficiency passively mode-locked Nd:YVO4 laser with direct in-band pumping at 914 nm.

We report on the performance of a semiconductor saturable absorber mirror passively mode-locked Nd:YVO4 laser with in-band pumping at 914 nm and with the highest slope efficiency to date among the mode-locked Nd-lasers. The laser produced 6.7 W of output power with repetition rate of 87 MHz and pulse duration of 16 ps. The slope efficiency of 77.1% and the optical-to-optical efficiency of 60.7% were achieved.

Femtosecond Kerr-lens mode-locked Alexandrite laser.

The generation of 170 fs pulses at 755 nm from a Kerr-lens mode-locked Alexandrite laser was demonstrated. The laser was pumped at 532 nm and produced 780 mW of average output power with 9.8% of optical-to-optical efficiency. To the best of our knowledge, these are the shortest pulses that have been produced from a mode-locked Alexandrite laser to date.

High-power continuous-wave dual-wavelength operation of a diode-pumped Yb:KGW laser.

High-power dual-wavelength diode-pumped Yb:KGW laser using a single birefringent filter plate was demonstrated. Two oscillating wavelengths maintained the same polarization and stable dual-wavelength operation at 1014.6 and 1041.3 nm (7.57 THz of frequency offset) with 3.4 W of average output power and a diffraction-limited beam profile was obtained. Dual-wavelength laser operation at shorter- or longer-wavelength pairs with lower average output power could also be realized for other output-coupling transmissions.

Dynamic characterization of intracavity losses in broadband quasi-three-level lasers.

We present a simple, fast and accurate technique to characterize intracavity losses in broadband quasi-three-level lasers based on spectroscopic gain analysis. The technique is based on spectral gain measurement and potentially can be used at any laser output power levels, thus allowing a dynamic optimization of laser performance. Successful experimental demonstration was carried out with a diode-pumped Yb:KGW continuous wave oscillator. A comparison with traditional Findlay-Clay analysis and numerical modeling was also made.

Quantitative nonlinear optical assessment of atherosclerosis progression in rabbits.

Quantification of atherosclerosis has been a challenging task owing to its complex pathology. In this study, we validated a quantitative approach for assessing atherosclerosis progression in a rabbit model using a numerical matrix, optical index for plaque burden, derived directly from the nonlinear optical microscopic images captured on the atherosclerosis-affected blood vessel. A positive correlation between this optical index and the severity of atherosclerotic lesions, represented by the age of the rabbits, was established based on data collected from 21 myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits with age ranging between new-born and 27 months old. The same optical index also accurately identified high-risk locations for atherosclerotic plaque formation along the entire aorta, which was validated by immunohistochemical fluorescence imaging.

Megawatt peak power level sub-100 fs Yb:KGW oscillators.

We report on the first demonstration, to the best of our knowledge, of sub-100 fs pulses directly from the diode-pumped mode-locked Yb:KGW bulk oscillators operated at a low repetition rate. The 36 MHz oscillator delivered 78 fs pulses with pulse energy of 50 nJ and peak power of 0.65 MW. The cavity was extended by inserting a 1:1 imaging telescope, allowing 85 fs pulses to be generated at a repetition rate of 18 MHz. The pulse energy up to 83 nJ was reached, corresponding to a peak power as high as 1 MW. Sub-100 fs regime was achieved by dual action of the Kerr-lens and saturable absorber (KLAS) mode locking.

Collagen morphology and texture analysis: from statistics to classification.

In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. Texture analysis based on first-order statistics (FOS) and second-order statistics such as gray level co-occurrence matrix (GLCM) was explored to extract second-harmonic generation (SHG) image features that are associated with the structural and biochemical changes of tissue collagen networks. Based on these extracted quantitative parameters, multi-group classification of SHG images was performed. With combined FOS and GLCM texture values, we achieved reliable classification of SHG collagen images acquired from atherosclerosis arteries with >90% accuracy, sensitivity and specificity. The proposed methodology can be applied to a wide range of conditions involving collagen re-modeling, such as in skin disorders, different types of fibrosis and muscular-skeletal diseases affecting ligaments and cartilage.