Microbial community profiling in intestinal tract of indigenous chickens from different villages.

Village chickens (Gallus gallus domesticus) are commonly reared in rural households of South Africa and other developing countries. They play a vital role as a primary source of protein through the provision of meat and eggs. The chicken gut microbiota plays an important role in chicken's immune system, its health, physiological development of the gut, digestion of food, nutrient absorption and productivity. Thus, it is imperative to critically investigate the chicken microbial composition in order to develop effective disease control measures and increase production. In this present study, microbial DNA was isolated from 34 non-descript mixed gender matured village chickens' intestinal contents followed by high throughput Illumina sequencing targeting 16S rRNA gene. Senwamokgope village had the largest microbiota composition as compared to Itieleni and Thakgalang villages. Overall, Firmicutes (74%) was the most abundant phylum observed, followed by Proteobacteria (8%), Actinobateria (5%), and Bacteroidota (3%). At the genus level, Lactobacillus was the dominant bacteria. Other genera found included Sphingomonas (7%), Cutibacterium (4%), and Clostridium_sensu_stricto_1 (2%). The richness of female intestinal microbiota was higher compared to the male microbiota. The findings of this study provide baseline information that can assist to better understand the chicken gut microbiota and its interaction with diseases and parasites.

A systematic review of ticks and tick-borne pathogens of cattle reared by smallholder farmers in South Africa.

Ticks are important ectoparasites of domestic animals, wild animals and humans. They spread a variety of infective agents such as protozoans, viruses, and bacteria. Cattle reared by smallholder farmers are susceptible to ticks and tick-borne pathogens due to the type of production system practiced by the farmers. Hence, this review was focused on the occurrence of ticks and tick-borne pathogens in cattle reared by smallholder farmers in South Africa. The systematic search produced a total of 13,408 articles from four databases, and after screening processes, the review utilized 23 articles published between 1983 and 2023. A total of 26 tick species belonging to seven genera were identified in the reviewed articles, with Rhipicephalus (Boophilus) decoloratus and Rhipicephalus evertsi evertsi being the most frequently reported tick species in South Africa followed by Amblyomma hebreum, Rhipicephalus appendiculatus, Hyalomma marginatum rufipes, Rhipicephalus microplus, Rhipicephalus follis, Rhipicephalus gertrudae and Hyalomma truncatum. The most frequently reported tick-borne pathogens across the provinces included Babesia bigemina, Babesia bovis, and Anaplasma marginale, with Eastern Cape Province accounting for most of the records followed by KwaZulu-Natal and Mpumalanga Provinces. The findings of this review confirm that cattle reared by smallholder farmers harbour various ticks and tick-borne pathogens of veterinary, public health and economic importance, and regular monitoring of tick infestations in South Africa is recommended to avoid disease outbreaks.

Phenotypic and genotypic characterization of ticks and tick-borne pathogens from cattle in selected villages of Greater Letaba Municipality in Limpopo Province, South Africa.

Ticks are blood ectoparasites that feed on domestic, wild animals and humans. They spread a variety of infections such as protozoa, viruses, and bacteria. Moreover, cattle reared by smallholder farmers are susceptible to ticks and tick-borne pathogens. Therefore, accurate identification of ticks and detection of tick-borne pathogens is crucial. The main aim of this study was to identify and characterize ticks and tick-borne pathogens from selected villages in Greater Letaba Municipality, Limpopo Province, using morphological and molecular techniques. A total of 233 ticks were collected from cattle and identified morphologically using appropriate morphological keys. The following tick species were identified: Amblyomma hebraeum, Hyalomma rufipes, Hyalomma truncatum, Rhipicephalus appendiculatus, Rhipicephalus (Boophilus) decoloratus, Rhipicephalus (Boophilus) microplus, Rhipicephalus evertsi evertsi, and Rhipicephalus sanguineus. Rhipicephalus spp. was the most common species accounting to 73.8% of the identified ticks. The genomic DNA was extracted from the whole tick for tick identification and from midguts of the ticks for the detection of tick-borne pathogens, followed by amplification and sequencing. A total of 27 samples were positive for tick-borne pathogens: 23 samples tested positive for Theileria and four samples tested positive for Ehrlichia. Anaplasma and Rickettsial OmpB could not be detected from any of the samples. There was no obvious grouping of ticks and tick-borne pathogens on the bases of their locality. The findings of this study confirm previous reports that indicated that cattle reared by smallholder farmers harbor various ticks and tick-borne pathogens of veterinary, public health, and economic importance. Regular monitoring of tick infestations in villages around the study areas is recommended to avoid disease outbreaks.

Single nucleotide polymorphisms of growth hormone gene in cattle and their association with growth traits: a systematic review.

Growth traits in livestock animals are quantitative parameters, which are often controlled by many genes including growth hormone (GH) gene. However, the evidence of effect of GH gene on growth traits of cattle is poorly understood. Hence, the objective of the study was to systematically investigate the literature on single nucleotide polymorphisms (SNPs) of GH gene and their association with growth traits in cattle from four databases Google Scholar, PubMed, ScienceDirect, and Web of Science. The results indicated that fifteen (n = 15) articles with 27% of them from Indonesia qualified to be used in this study after screening. The results revealed five SNPs (1047T > C, 1180 C > T, 86,273,136 A/G, 3338 A > G and 4251 C > T) occurred across multiple investigated breeds with no common identified SNPs. Six articles observed a significant difference (p < 0.05) between growth traits and genotypes of identified SNPs. The findings showed that 7 articles (47%) investigated body weight (BW) with 6 (40%) of them found non-significant and 1 (7%) found a significant association with genotypes of the identified SNPs (3338 A > G). While 7 articles (47%) investigated weaning weight (WW) with 5 (33%) of them revealed a non-significant and 2 (13%) found a significant association with genotypes of identified SNPs (3338 A > G and 4251 C > T). This study shows that there is a lack of evidence on effect of growth hormone gene on growth traits in cattle. However, more studies are recommended for further validation of the identified SNPs and effect of growth hormone gene on growth traits in cattle.

Morphological characterization of Sussex cattle at Huntersvlei farm, Free State Province, South Africa.

Sussex cattle breed is characterized by their distinctive solid red coat colour and white tail switch. Sussex cattle are known for being easy to handle and manage, making them an ideal choice for cattle farmers. The phenotypic characterization of this cattle breed in South Africa is unknown. Hence, the objective of this study was to characterize the morphological structure, phenotypic and body indices traits of Sussex cattle in South Africa at Huntersvlei farm, Frere State province of South Africa. One hundred and one weaners (n = 101) between 6 and 8 months old (female = 57 and male = 44) and fifty yearlings between 12 and 15 months old (female = 15 and male = 35) were used in this study. Body weight at weaning, yearling and linear body measurements such as head length (HL), head width (HW), ear length (EL), ear width (EW), sternum height (SH), withers height (WH), heart girth (HG), hip height (HH), body length (BL), rump length (RL), and rump width (RW) were measured. Moreover, the animals were assessed for coat colour and horn presence. Descriptive statistics, Pearson's correlation and Principal Component Analysis (PCA) were used to describe the Sussex cattle breed. The results indicated that male Sussex cattle had highly significant (p < 0.01) mean numeric values for the BW and morphometric traits. The results further showed that Sussex cattle had highly significant (p < 0.01) increase for the BW and morphometric traits as age advances in all sexes. Interaction effect of sex and age showed a highly significant (p < 0.01) effect with BW and measured morphometric traits, while moderately significant (p < 0.05) with EW. Male Sussex cattle showed highly significant (p < 0.01) higher mean numeric values for the body index (BI), length index (LI) and compact index (CI) indices. While female Sussex animals showed highly significant (p < 0.01) mean numeric values for area index (AI) and proportionality (PR). Body weight showed a positive highly significant (p < 0.01) correlation with the measured morphometric traits except for the moderate significant (p < 0.05) correlation with EL. Coat colour traits ranged from 15 (9.93%), 103 (68.21%) to 33 (21.85%) for light, moderate and dark colours, respectively. While horn presence traits ranged from 48 (31.79%), 42 (27.81%) to 61 (40.40%) for polled, scur and horned respectively. The PCA results extracted only two components in both sexes of the animals. The morphological variations obtained in this study could be complemented by performance data and molecular markers of single nucleotide polymorphism (SNP) to guide the overall breed characterization, conservation and development of appropriate breeding and selection strategies.