RESUMO
This work is the first report dealing with the identification and characterisation of the secondary metabolites of the ethanolic extract of Hydrocharis Laevigata (Humb. & Bonpl. Ex Willd.) Byng & Christenh. The ethanolic extract of H. laevigata was analysed by LCMS& Direct mass spectral analysis which is allowed to identify and Interpreted 6 & 15 compounds. The main constituents were caffeic acid, rosemary acid, Perilic acid, strychnine, hydroxy stearic acid, respectively. The extract further purified by column chromatography 15 fractions was isolated, out of which Perilic acid and strychnine are in high quantities. The structure determination of Perilic acid and strychnine was analysed by FTIR and NMR, respectively. By the molecular docking studies of Perilic acid and strychnine shows active binding energies for antidiabetic activity, respectively. The binding energy was compared with Metformin.
RESUMO
Materials based on carbohydrate polymers may be used for biomedical application. However, materials based on natural polymers have weak physicochemical properties. Thereby, there is a challenge to improve their properties without initiation of toxicity. The alternative method compared to toxic chemical agents' addition is the use of metal complexation method. In this study, chitosan/tannic acid mixtures modified by Fe(III) complexation are proposed and tested for potential applications as wound dressings. Thereby, surface properties, blood compatibility as well as platelet adhesion was tested. In addition, the periodontal ligament stromal cells compatibility studies were carried out. The results showed that the iron(III) addition to chitosan/tannic acid mixture improves properties due to a decrease in the surface free energy and exhibited a reduction in the hemolysis rate (below 5%). Moreover, cells cultured on the surface of films with Fe(III) showed higher metabolic activity. The current findings allow for the medical application of the proposed materials as wound dressings.