Design and Validation of Single-Axis 3D-Printed Force Sensor Based on Three Nested Flexible Rings.

Force measurement is crucial in numerous engineering applications, while traditional force sensors often face problems such as elevated expenses or significant measurement errors. To tackle this issue, we propose an innovative force sensor employing three nested flexible rings fabricated through 3D additive manufacturing, which detects external forces through the displacement variations of flexible rings. An analytical model on the basis of the minimal energy method is developed to elucidate the force-displacement correlation with nonlinearity. Both FEM simulations and experiments verify the sensor's effectiveness. This sensor has the advantages of low expenses and easy manufacture, indicating promising prospects in a range of applications, including robotics, the automotive industry, and iatrical equipment.

Activating peroxymonosulfate with Fe-doped biochar for efficient removal of tetracycline: Dual action of reactive oxygen species and electron transfer.

If pharmaceutical wastewater is not managed effectively, the presence of residual antibiotics will result in significant environmental contamination. In addition, inadequate utilization of agricultural waste represents a squandering of resources. The objective of this research was to assess the efficacy of iron-doped biochar (Fe-BC) derived from peanut shells in degrading high concentrations of Tetracycline (TC) wastewater through activated peroxymonosulfate. Fe-BC demonstrated significant efficacy, achieving a removal efficiency of 87.5% for TC within 60 min without the need to adjust the initial pH (20 mg/L TC, 2 mM PMS, 0.5 g/L catalyst). The degradation mechanism of TC in this system involved a dual action, namely Reactive Oxygen Species (ROS) and electron transfer. The primary active sites were the Fe species, which facilitated the generation of SO4•-, •OH, O2•-, and 1O2. The presence of Fe species and the C=C structure in the Fe-BC catalyst support the electron transfer. Degradation pathways were elucidated through the identification of intermediate products and calculation of the Fukui index. The Toxicity Estimator Software Tool (T.E.S.T.) suggested that the intermediates exhibited lower levels of toxicity. Furthermore, the system exhibited exceptional capabilities in real water and circulation experiments, offering significant economic advantages. This investigation provides an efficient strategy for resource recycling and the treatment of high-concentration antibiotic wastewater.

Serotonin receptor 2B induces visceral hyperalgesia in rat model and patients with diarrhea-predominant irritable bowel syndrome.

BACKGROUND: Serotonin receptor 2B (5-HT2B receptor) plays a critical role in many chronic pain conditions. The possible involvement of the 5-HT2B receptor in the altered gut sensation of irritable bowel syndrome with diarrhea (IBS-D) was investigated in the present study. AIM: To investigate the possible involvement of 5-HT2B receptor in the altered gut sensation in rat model and patients with IBS-D. METHODS: Rectosigmoid biopsies were collected from 18 patients with IBS-D and 10 patients with irritable bowel syndrome with constipation who fulfilled the Rome IV criteria and 15 healthy controls. The expression level of the 5-HT2B receptor in colon tissue was measured using an enzyme-linked immunosorbent assay and correlated with abdominal pain scores. The IBS-D rat model was induced by intracolonic instillation of acetic acid and wrap restraint. Alterations in visceral sensitivity and 5-HT2B receptor and transient receptor potential vanilloid type 1 (TRPV1) expression were examined following 5-HT2B receptor antagonist administration. Changes in visceral sensitivity after administration of the TRPV1 antagonist were recorded. RESULTS: Here, we observed greater expression of the 5-HT2B receptor in the colonic mucosa of patients with IBS-D than in that of controls, which was correlated with abdominal pain scores. Intracolonic instillation of acetic acid and wrap restraint induced obvious chronic visceral hypersensitivity and increased fecal weight and fecal water content. Exogenous 5-HT2B receptor agonist administration increased visceral hypersensitivity, which was alleviated by successive administration of a TRPV1 antagonist. IBS-D rats receiving the 5-HT2B receptor antagonist exhibited inhibited visceral hyperalgesia.Moreover, the percentage of 5-HT2B receptor-immunoreactive (IR) cells surrounded by TRPV1-positive cells (5-HT2B receptor I+) and total 5-HT2B receptor IR cells (5-HT2B receptor IT) in IBS-D rats was significantly reduced by the administration of a 5-HT2B receptor antagonist. CONCLUSION: Our finding that increased expression of the 5-HT2B receptor contributes to visceral hyperalgesia by inducing TRPV1 expression in IBS-D patients provides important insights into the potential mechanisms underlying IBS-D-associated visceral hyperalgesia.

Biological characteristics of molecular subtypes of ulcerative colitis characterized by ferroptosis and neutrophil infiltration.

Clinical ulcerative colitis (UC) is a heterogeneous condition. Moreover, medical interventions are nonspecific, and thus, treatment responses are inconsistent. The aim of this study was to explore the molecular subtypes and biological characteristics of UC based on ferroptosis and neutrophil gene sets. Multiple intestinal mucosa gene expression profiles of UC patients in the Gene Expression Omnibus (GEO) database were downloaded. Unsupervised clustering methods were used to identify potential molecular subtypes based on ferroptosis and neutrophil gene sets. Multiple immune infiltration algorithms were used to evaluate the biological characteristics of the molecular subtypes. Machine learning identifies hub genes for molecular subtypes and analyses their diagnostic efficacy for UC and predictive performance for drug therapy. The relevant conclusions were verified by clinical samples and animal experiments. Four molecular subtypes were identified according to the ferroptosis and neutrophil gene sets: neutrophil, ferroptosis, mixed and quiescent. The subtypes have different biological characteristics and immune infiltration levels. Multiple machine learning methods jointly identified four hub genes (FTH1, AQP9, STEAP3 and STEAP4). Receiver operating characteristic (ROC) curve analysis revealed that the four hub genes could be used as diagnostic markers for UC. The clinical response profile data of infliximab treatment patients showed that AQP9 and STEPA4 were reliable predictors of infliximab treatment response. In human samples the AQP9 and STEAP4 protein were shown to be increased in UC intestinal samples. In animal experiments, the ferroptosis and neutrophil phenotype were confirmed. Dual analysis of ferroptosis and neutrophil gene expression revealed four subgroups of UC patients. The molecular subtype-associated hub genes can be used as diagnostic markers for UC and predict infliximab treatment response.

Combined transcriptomics and metabolomics to analyse the response of Cuminum cyminum L. under Pb stress.

Lead (Pb) can disrupt plant gene expression, modify metabolite contents, and influence the growth of plants. Cuminum cyminum L. is highly adaptable to adversity, but molecular mechanism by which it responds to Pb stress is unknown. For this study, transcriptomic and metabolomic sequencing was performed on root tissues of C. cyminum under Pb stress. Our results showed that high Pb stress increased the activity of peroxidase (POD), the contents of malondialdehyde (MDA) and proline by 80.03 %, 174.46 % and 71.24 %, respectively. Meanwhile, Pb stress decreased the activities of superoxide dismutase (SOD) and catalase (CAT) as well as contents of soluble sugars and GSH, which thus affected the growth of C. cyminum. In addition, Pb stress influenced the accumulation and transport of Pb in C. cyminum. Metabolomic results showed that Pb stress affected eight metabolic pathways involving 108 differentially expressed metabolites, primarily amino acids, organic acids, and carbohydrates. The differentially expressed genes identified through transcriptome analysis were mainly involved the oxidation reductase activity, transmembrane transport, phytohormone signaling, and MAPK signaling pathway. The results of this study will help to understand the molecular mechanisms of C. cyminum response to Pb stress, and provide a basis for screening seeds with strong resistance to heavy metals.

Building Environmental and Sociological Predictive Intelligence to Understand the Seasonal Threat of SARS-CoV-2 in Human Populations.

Current modeling practices for environmental and sociological modulated infectious diseases remain inadequate to forecast the risk of outbreak(s) in human populations, partly due to a lack of integration of disciplinary knowledge, limited availability of disease surveillance datasets, and overreliance on compartmental epidemiological modeling methods. Harvesting data knowledge from virus transmission (aerosols) and detection (wastewater) of SARS-CoV-2, a heuristic score-based environmental predictive intelligence system was developed that calculates the risk of COVID-19 in the human population. Seasonal validation of the algorithm was uniquely associated with wastewater surveillance of the virus, providing a lead time of 7-14 days before a county-level outbreak. Using county-scale disease prevalence data from the United States, the algorithm could predict COVID-19 risk with an overall accuracy ranging between 81% and 98%. Similarly, using wastewater surveillance data from Illinois and Maryland, the SARS-CoV-2 detection rate was greater than 80% for 75% of the locations during the same time the risk was predicted to be high. Results suggest the importance of a holistic approach across disciplinary boundaries that can potentially allow anticipatory decision-making policies of saving lives and maximizing the use of available capacity and resources.

Endogenously Gated DNA Walking Machine for Prescreened MicroRNA Detection in Extracellular Vesicles.

Tumor-derived extracellular vesicle (T-EV) microRNAs have been investigated as promising biomarkers in clinical diagnosis as well as disease progression monitoring. However, the expression profiles of microRNA in different tissues vary widely, the precise monitoring of microRNA levels in EVs originating from diseased tissues is susceptible to background interference, thus remains a challenge. Conventional assays require extensive processing, such as EV isolation and even sample lysis, which is both slow and laborious, and the cumbersome pretreatment could spoil the downstream analysis. To address this issue, we developed a generalizable strategy for T-EVs-selective activation and therefore specific amplified microRNA imaging. The conditional signal amplification is established by integrating a traditional DNA walker system with endogenously activated motif to achieve sensitized microRNA imaging in T-EVs. The preorganized endogenous activation with additional sensing criteria narrowed the scope against the complex specimens, and the amplified sensing with reduced off-target signals was supposed to be sensitive to monitor the tiny changes of microRNA expression during the disease course, which holds great potential for accurate diagnosis and prognosis.

Combining brushing cytology with simultaneous vacuum aspiration in suspicious biliopancreatic malignancies: A multicenter propensity score-matched study (with video).

BACKGROUND: Brush cytology during endoscopic retrograde cholangiopancreatography (ERCP) is a standard approach in diagnosing biliopancreatic strictures, with yet unsatisfying sensitivity. AIMS: We brought additional simultaneous vacuum aspiration to brushing process and re-evaluate the diagnostic performance. METHODS: This multi-centered retrospective study was conducted in three tertiary centers. Consecutive patients with biliopancreatic strictures were identified. The patients were divided into two arms: the conventional arm (CA) receiving general brushing approach, and the modified arm (MA) being treated with additional vacuum aspiration when performing bushing. The 1:1 propensity-score matching was implemented to tackle the selective biases. RESULTS: A total of 555 patients were identified and 200 patient pairs (193 males, 207 females, with a mean age of 68.1 ± 13.1 years.) fell into the ultimate evaluation. A final diagnosis of malignant stricture was established in 243 patients. The diagnostic yield of the MA group was substantially better than that of the CA group, whether "suspicious malignancies" were considered malignancies or not. The rates of sensitivity, specificity and accuracy were 46.2%, 100%, 68.0% in the MA group, and 15.3%, 98.7%, and 47.0% in the CA group respectively. CONCLUSIONS: Brushing accompanied by simultaneous vacuum aspiration at ERCP improves the diagnostic yield in suspicious biliopancreatic malignancies.

Local and Environmental Reservoirs of Salmonella enterica After Hurricane Florence Flooding.

In many regions of the world, including the United States, human and animal fecal genetic markers have been found in flood waters. In this study, we use high-resolution whole genomic sequencing to examine the origin and distribution of Salmonella enterica after the 2018 Hurricane Florence flooding. We specifically asked whether S. enterica isolated from water samples collected near swine farms in North Carolina shortly after Hurricane Florence had evidence of swine origin. To investigate this, we isolated and fully sequenced 18 independent S. enterica strains from 10 locations (five flooded and five unflooded). We found that all strains have extremely similar chromosomes with only five single nucleotide polymorphisms (SNPs) and possessed two plasmids assigned bioinformatically to the incompatibility groups IncFIB and IncFII. The chromosomal core genome and the IncFIB plasmid are most closely related to environmental Salmonella strains isolated previously from the southeastern US. In contrast, the IncFII plasmid was found in environmental S. enterica strains whose genomes were more divergent, suggesting the IncFII plasmid is more promiscuous than the IncFIB type. We identified 65 antibiotic resistance genes (ARGs) in each of our 18 S. enterica isolates. All ARGs were located on the Salmonella chromosome, similar to other previously characterized environmental isolates. All isolates with different SNPs were resistant to a panel of commonly used antibiotics. These results highlight the importance of environmental sources of antibiotic-resistant S. enterica after extreme flood events.

Clinical Efficiency of Fixed-Bearing Unicompartmental Knee Arthroplasty Versus Total Knee Arthroplasty For Lateral Compartment Knee Osteoarthritis and the Effect on Recovery of Motor Function.

Objective: To evaluate the clinical efficiency of fixed-bearing unicompartmental knee arthroplasty (UKA) versus total knee arthroplasty (TKA) for lateral compartment knee osteoarthritis and the effect on the recovery of motor function. Methods: A total of 54 patients who underwent surgery for lateral compartment knee osteoarthritis and satisfied the inclusion criteria from September 2018 to February 2021 at our hospital were recruited and assigned to receive either UKA (UKA group, n=30) or TKA (TKA group, n=24) via random number table method. Among them, the randomization was carried out using an online web-based randomization tool (freely available at http://www.randomizer.org/). Inclusion criteria: 1) patients with lateral compartment knee osteoarthritis diagnosed by clinically relevant tests; 2) patients with structural and functional integrity of the knee ligaments; 3) all with a single knee lesion. Outcome measures included operative time, the reduction ratio of Hb 1d postoperatively, visual analog scale (VAS) score 7d postoperatively, length of hospital stay, postoperative Keen society score (KSS), Oxford knee score (OKS), range of motion (ROM), forgotten joint score (FJS), motor function recovery, and adverse events. Results: All patients were followed up postoperatively for 12-33 (21.71±7.45) months. Patients in the UKA group showed significantly shorter operative indices, a lower reduction ratio of Hb 1d postoperatively, and VAS scores of 7d postoperatively (P < .05). At 1 month and 6 months postoperatively, UKA resulted in significantly better KSS scores, OKS scores, ROM, and motor function recovery versus TKA (P < .05), while the difference of the above indices did not come up to the statistical standard at 1 year postoperatively (P < .05). At 1 year postoperatively, patients receiving UKA were associated with significantly higher FJS scores versus those given TKA. No documented thrombosis, knee, or prosthesis-related adverse events were observed during hospitalization and follow-up. Conclusion: In comparison to TKA, UKA resulted in smaller surgical incisions, improved postoperative healing, and greater restoration of knee function. Both arthroplasties are successful in alleviating pain and increasing knee function, although they are less effective in recovering patients' motor capabilities.

Assessment of pathogens in flood waters in coastal rural regions: Case study after Hurricane Michael and Florence.

The severity of hurricanes, and thus the associated impacts, is changing over time. One of the understudied threats from damage caused by hurricanes is the potential for cross-contamination of water bodies with pathogens in coastal agricultural regions. Using microbiological data collected after hurricanes Florence and Michael, this study shows a dichotomy in the presence of pathogens in coastal North Carolina and Florida. Salmonella typhimurium was abundant in water samples collected in the regions dominated by swine farms. A drastic decrease in Enterococcus spp. in Carolinas is indicative of pathogen removal with flooding waters. Except for the abundance presence of Salmonella typhimurium, no significant changes in pathogens were observed after Hurricane Michael in the Florida panhandle. We argue that a comprehensive assessment of pathogens must be included in decision-making activities in the immediate aftermath of hurricanes to build resilience against risks of pathogenic exposure in rural agricultural and human populations in vulnerable locations.

Two complementary AI approaches for predicting UMLS semantic group assignment: heuristic reasoning and deep learning.

OBJECTIVE: Use heuristic, deep learning (DL), and hybrid AI methods to predict semantic group (SG) assignments for new UMLS Metathesaurus atoms, with target accuracy ≥95%. MATERIALS AND METHODS: We used train-test datasets from successive 2020AA-2022AB UMLS Metathesaurus releases. Our heuristic "waterfall" approach employed a sequence of 7 different SG prediction methods. Atoms not qualifying for a method were passed on to the next method. The DL approach generated BioWordVec and SapBERT embeddings for atom names, BioWordVec embeddings for source vocabulary names, and BioWordVec embeddings for atom names of the second-to-top nodes of an atom's source hierarchy. We fed a concatenation of the 4 embeddings into a fully connected multilayer neural network with an output layer of 15 nodes (one for each SG). For both approaches, we developed methods to estimate the probability that their predicted SG for an atom would be correct. Based on these estimations, we developed 2 hybrid SG prediction methods combining the strengths of heuristic and DL methods. RESULTS: The heuristic waterfall approach accurately predicted 94.3% of SGs for 1 563 692 new unseen atoms. The DL accuracy on the same dataset was also 94.3%. The hybrid approaches achieved an average accuracy of 96.5%. CONCLUSION: Our study demonstrated that AI methods can predict SG assignments for new UMLS atoms with sufficient accuracy to be potentially useful as an intermediate step in the time-consuming task of assigning new atoms to UMLS concepts. We showed that for SG prediction, combining heuristic methods and DL methods can produce better results than either alone.

The microalgae-based wastewater treatment system coupled with Cerium: A potential way for energy saving and microalgae boost.

The microalgae-based system attracts more attention in wastewater treatment for high quality effluent, low carbon emission, and resource utilization. Light is the key factor for algae growth, but the light masking in sewage will cause low efficiency of the system. This study designed laboratory scale experiments with Chlorella to investigate the influence of cerium on the nutrient removal by algae wastewater treatment system under different light intensities. The best removal rates of NH4-N, TP, and COD were 72.43%, 88.87%, and 68.08% under 50 µmol/(m 2·s) light intensity and 1 mg/L Ce. Low concentration of Ce could activate protein synthesis, electron transfer, and antioxidase, while excessive Ce might cause toxicity which could be relieved by strong light for energy supply and further activating superoxide dismutase (SOD) and catalase (CAT). Comparing to other similar experiences, this system reached an equal or greater performance on nutrients removal with better efficiency in light utilization. It might provide a new idea for microalgae-based system development.

Syntrichia caninervis adapt to mercury stress by altering submicrostructure and physiological properties in the Gurbantünggüt Desert.

Sewage and industrial waste discharges have been found to have a deleterious effect on plant growth and environmental safety through the accumulation of trace metal mercury (Hg) in soils. Although the effects of Hg on vascular plants have been reported in terms of enzyme activity, oxidative damage and physiology, few studies have been done on non-vascular plants. A simulation experiment including 7 Hg concentrations (0, 10, 20, 30, 40, 50, 75 µM) was conducted to investigate the influence of Hg stress on ultrastructure and physiological properties of biocrust moss Syntrichia. caninervis across 7 consecutive days. The results showed that the lowest lethal concentration of S. caninervis was 30 µM Hg. The mortality rate of the plants increased significantly with Hg concentrations. The ultrastructure did not change significantly at Hg concentration ≤ 20 µM, while exceeding which, cell walls began to separate, nuclei began to blur, and chloroplasts began to expand. The soluble sugars (SS), peroxidase (POD), and superoxide dismutase (SOD) activities increased initially and then decreased with the increase of concentration in the time gradient, with the largest values at 20 µM. The contents of malondialdehyde (MDA) and proline (Pro) increased with the increase of Hg concentration, both reached peak value at 50 µM. However, chlorophyll (Chl) contents continued to decrease along both the concentration and time gradients. Pearson correlation and principal component analysis showed that two principal components (PC1 and PC2) explained 73.9% of the variance in plant adaptation to Hg stress. SOD, POD, Chl, SS, and Pro all responded well to Hg in S. caninervis. Our study showed that Hg stress caused changes in ultrastructure and physiological metabolism of S. caninervis. 20 µM was the maximum concentration of Hg that biocrust moss S. caninervis can tolerate. S. caninervis mainly adopted two adaptation strategies related to exclusion and accumulation to reduce Hg stress.

Biochar Addition Alters C: N: P Stoichiometry in Moss Crust-Soil Continuum in Gurbantünggüt Desert.

The biogeochemical cycling of soil elements in ecosystems has changed under global changes, including nutrients essential for plant growth. The application of biochar can improve the utilization of soil nutrients by plants and change the stoichiometry of carbon (C), nitrogen (N), and phosphorus (P) in plants and soil. However, the response of ecological stoichiometry in a moss crust-soil continuum to local plant biochar addition in a desert ecosystem has not been comprehensively explored. Here, we conducted a four-level Seriphidium terrae-albae biochar addition experiment (CK, 0 t ha−1; T1, 3.185 t ha−1; T2, 6.37 t ha−1; T3, 12.74 t ha−1) to elucidate the influence of biochar input on C: N: P stoichiometry in moss crusts (surface) and their underlying soil (subsurface). The results showed that biochar addition significantly affected the C, N, and P both of moss crusts and their underlying soil (p < 0.001). Biochar addition increased soil C, N, and P concentrations, and the soil N content showed a monthly trend in T3. The C, N, and P concentrations of moss crusts increased with the addition levels of biochar, and the moss crust P concentrations showed an overall increasing trend by the month. Moreover, the soil and moss crust C: P and N: P ratios both increased. There was a significant correlation between moss crust C, N, and P and soil C and N. Additionally, nitrate nitrogen (NO3−N), N: P, C: P, EC, pH, soil moisture content (SMC), and N have significant effects on the C, N, and P of moss crusts in turn. This study revealed the contribution of biochar to the nutrient cycle of desert system plants and their underlying soil from the perspective of stoichiometric characteristics, which is a supplement to the theory of plant soil nutrition in desert ecosystems.

Effect of Welding Speed on Microstructure Evolution and Mechanical Properties of Friction Stir Welded 2198 Al-Cu-Li Alloy Joints.

In the present study, 2198 Al-Cu-Li alloys were successfully friction stir welded by using various welding speed ranges of 90~180 mm/min with an invariable rotation speed of 950 r/min. The effect of welding speed on microstructure evolution and mechanical properties of the joints was investigated. The results show that, with the welding speed decreasing, the size of the nugget zone (NZ) first increases and then decreases due to different welding temperatures. At a welding speed of 150 mm/min, the size of the NZ in all joints is the biggest and the "S" curve disappears. The equiaxed grains are finer, attributed to a higher degree of dynamic recrystallization, and a larger number of fine reprecipitated phase (δ', ß' phases) particles are dispersively distributed in the NZ. Correspondingly, the joints have the highest tensile properties, and the tensile strength, yield strength and elongation are, respectively, 406 MPa, 289 MPa and 7.2%. However, compared to the base material, the tensile properties of all joints are reduced because a greater amount of δ' and ß' phases particles are dissolved in the NZ. Only the joints produced at 150 mm/min are fractured in the TMAZ with detected deep dimples and tearing ridges, and a significant necking phenomenon is observed, which indicates a complete ductile fracture mode.