Genome-centric metagenomes unveiling the hidden resistome in an anchialine cave.

BACKGROUND: Antibiotic resistance is a critical global concern, posing significant challenges to human health and medical treatments. Studying antibiotic resistance genes (ARGs) is essential not only in clinical settings but also in diverse environmental contexts. However, ARGs in unique environments such as anchialine caves, which connect both fresh and marine water, remain largely unexplored despite their intriguing ecological characteristics. RESULTS: We present the first study that comprehensively explores the occurrence and distribution of ARGs and mobile genetic elements (MGEs) within an anchialine cave. Utilizing metagenomic sequencing we uncovered a wide array of ARGs with the bacitracin resistance gene, bacA and multidrug resistance genes, being the most dominant. The cave's microbial community and associated resistome were significantly influenced by the salinity gradient. The discovery of novel ß-lactamase variants revealed the cave's potential as a reservoir for previously undetected resistance genes. ARGs in the cave demonstrated horizontal transfer potential via plasmids, unveiling ecological implications. CONCLUSIONS: These findings highlight the need for further exploration of the resistome in unique environments like anchialine caves. The interconnected dynamics of ARGs and MGEs within anchialine caves offer valuable insights into potential reservoirs and mechanisms of antibiotic resistance in natural ecosystems. This study not only advances our fundamental understanding but also highlights the need for a comprehensive approach to address antibiotic resistance in diverse ecological settings.

Phytoplankton pigment dynamics in marine lake fluctuating between stratified and holomictic euxinic conditions.

Biomass dynamics in the marine lake are strongly dependent on seasonal variability in vertical stratification, indicating rapid adaptation of phytoplankton to short-term changes in the water column. A small marine lake (Rogoznica Lake, Croatia), which fluctuates between stably stratified and holomictic euxinic conditions, was used as a model to study the phytoplankton responses to environmental perturbations, in particular the anoxic stress, caused by periodic holomixia. The epilimnion showed significant temporal and vertical variability with a chlorophyll a subsurface maximum with the highest biomass near the chemocline. Fucoxanthin-containing biomass (diatoms) dominated in the epilimnion in colder seasons and was first to recover after holomictic euxinic events. The shift towards the smaller groups prevailed during highly stratified water column conditions in warmer seasons. Results for the hypolimnion were more enigmatic, with high concentrations of alloxanthin, zeaxanthin, and violaxanthin indicating the presence of a viable small-size mixotrophic community under extreme conditions.

Dynamics of organic matter in the changing environment of a stratified marine lake over two decades.

The marine lake (Rogoznica Lake), which fluctuates between stratified and holomictic conditions, is a unique environment on the eastern Adriatic coast affected by environmental changes. These changes are reflected in the warming of the water column, the apparent deoxygenation of the epilimnion, and the accumulation of organic matter (OM), toxic sulfide, and ammonium in the anoxic hypolimnion. Since the early 1990s, the volume of anoxic water has increased as the chemocline has moved to the surface water layer. A trend toward enrichment of refractory dissolved organic carbon (DOC) was observed in the anoxic hypolimnion, while a decreasing trend was observed in the oxic epilimnion in the spring DOC. At the same time, the most reactive surface-active fraction of DOC showed the opposite trend. In addition, there is evidence of accumulation of particulate organic carbon (POC) in the water column, followed by an increase in the fraction of POC in total organic carbon (TOC). On a multi-year scale (1996-2020), this work presents a unique time series of the dynamics of OM in the stratified marine system, showing a significant change in its quantity and quality due to climate and environmental variability. DOC-normalized surfactant activity is shown to be a good indicator of environmental change.

Chronoamperometric study of elemental sulphur (S) nanoparticles (NPs) in NaCl water solution: new methodology for S NPs sizing and detection.

BACKGROUND: Elemental sulfur (S) persists in natural aquatic environment in a variety of forms with different size distributions from dissolved to particulate. Determination of S speciation mainly consists of the application of chromatographic and electrochemical techniques while its size determination is limited only to the application of microscopic and light scattering techniques. S biological and geochemical importance together with recent increases of S industrial applications requires the development of different analytical tools for S sizing and quantification. In recent years the use of electrochemical measurements as a direct, fast, and inexpensive technique for the different nanoparticles (NPs) characterization (Ag, Au, Pt) is increasing. In this work, electrochemical i.e. chronoamperometric measurements at the Hg electrode are performed for determination of the size distribution of the S NPs. RESULTS: S NPs were synthesized in aqueous medium by sodium polysulphide acidic hydrolysis. Chronoamperometric measurements reveal the polydisperse nature of the formed suspension of S NPs. The electrochemical results were compared with dynamic light scattering measurements parallel run in the same S NPs suspensions. The two methods show fairly good agreement, both suggesting a log-normal size distribution of the S NPs sizes characterized by similar parameters. CONCLUSIONS: The preliminary results highlight the amperometric measurements as a promising tool for the size determination of the S NPs in the water environment.