RESUMO
We studied the effect of microvesicles derived from cells of the NK-92 cell line on the formation of tube-like structures by endothelial cells of the ÐÐ.Hy926 cell line. Microvesicles were isolated by differential centrifugation and their size was controlled by granulometric analysis using dynamic light scattering method. The effect of microvesicles produced by NK cells on angiogenesis was evaluated by cultural methods. In the course of the research, a model of co-culturing of microvesicles and endothelial cells on extracellular matrix Matrigel was developed. It was found that microvesicles derived from NK-92 cells promoted elongation of tube-like structures formed by endothelial ÐÐ.Hy926 cells. Microvesicles produced by NK cells can modulate functional activity of endothelial cells by affecting their ability to form blood vessels.
Assuntos
Micropartículas Derivadas de Células/química , Meios de Cultura/química , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Matadoras Naturais/química , Neovascularização Fisiológica , Linhagem Celular , Linhagem Celular Tumoral , Técnicas de Cocultura , Colágeno/química , Meios de Cultura/farmacologia , Combinação de Medicamentos , Células Endoteliais da Veia Umbilical Humana/citologia , Células Endoteliais da Veia Umbilical Humana/fisiologia , Humanos , Células Matadoras Naturais/citologia , Células Matadoras Naturais/metabolismo , Laminina/química , Modelos Biológicos , Proteoglicanas/químicaRESUMO
Morphological properties and the size of microvesicles were assessed using atomic force microscopy, electron microscopy, and granulometric analysis. As these methods require significant numbers of microvesicles, we chose microvesicles derived from cell lines for our research.