Reduction of aerosol dissemination in a dental area generated by high-speed and scaler ultrasonic devices employing the "Prime Protector".

The use of an external dome aerosol containment device (Prime Protector) is proposed to reduce the spread of particles within the dental office. Hence, the aim of our study was to compare the spread of bioaerosols generated by a High-speed Handpiece (HH) and an Ultrasonic Prophylaxis Device (UPD), with and without the Prime Protector dome (PP) by counting Colony Forming Units (CFU) of Lactobacillus casei Shirota, at different distances on the x and y axis. The PP was located considering the parallelism between the base of the dome and the frontal plane of the simulator, aligning the center of the mouth with the center of the dome. The PP dome measurements are 560.0mm x 255.0mm x 5mm. Petri dishes were placed at 0.5 m, 1 m and 1.5 m respectively. Aerosol generation in the laboratory environment was done three times with the following experimental groups 1) HH, 2) HH-PP, 3) UPD, 4) UPD-PP. Each dental device activation (HH and UPD) had a time frame of 2 minutes on the upper anterior teeth of the dental phantom with a liquid suspension containing Lactobacillus casei Shirota (YAKULT 0836A 0123; 1027F 0407). Air pressure and ventilation were parameterized. No separate high-volume evacuation used, nor was there any air removal attached to the dome. Results showed no significant difference between distance and axis in the CFU count. When means for devices and distances were compared between each of them all showed significant differences except for UPD and UPD-PP (p <0,004). In conclusion, external devices like Prime Protector could help decrease aerosol diffusion during high-speed handpiece activation. However, this dome does not replace the use of PPE inside dental clinics.

Analysis of hippocampus in rats with acute brain ischemia-reperfusion injury treated with leuprolide acetate, an agonist of GnRH.

BACKGROUND: The hippocampus is highly vulnerable to damage in the brain ischemia-reperfusion injury model. Leuprolide acetate has been shown to promote neurological recovery after injury in various regions of the central nervous system. OBJECTIVE: The objective of this study was to assess the histology of the hippocampus and the expression of neuronal recovery markers, specifically the 200 kDa neurofilaments and the myelin basic protein, in rats with brain ischemia-reperfusion injury treated with leuprolide acetate. METHODS: The rats were divided into three groups: Sham, ischemia-reperfusion with saline solution, and ischemia-reperfusion treated with leuprolide acetate. Coronal brain slices were obtained and stained with hematoxylin-eosin. The histological analysis involved quantifying the number of neurons in the hippocampal regions CA1, CA3 and DG. The myelin basic protein and neurofilaments were quantified using western blot. RESULTS: The number of neurons in CA1 and DG was significantly higher in the leuprolide acetate group compared to the untreated group. Additionally, the expression of neurofilament and myelin basic protein markers was significantly increased in rats treated with leuprolide acetate compared to the untreated rats. CONCLUSIONS: Leuprolide acetate promotes the recovery of hippocampal neurons in an acute brain ischemia-reperfusion injury model. These findings suggest that leuprolide acetate could be a potential therapeutic intervention for reversing damage in hippocampal ischemic lesions.

[Family functioning, parental rearing and its relationship to nutritional status in preschoolers]. / Funcionalidad familiar, crianza parental y su relación con el estado nutricional en preescolares.

AIM: To analyze association among family functioning, parental rearing and nutritional status in preschoolers. DESIGN: Quantitative, descriptive and correlational cross-sectional study. LOCATION: Preschool public institutions in Nuevo León, Mexico. Primary Care. PARTICIPANTS: Sample of 228 dyads (mother or father and preschooler); sole inclusion criterion was to be main responsible for the upbringing of the preschooler. MAIN MEASUREMENTS: Sociodemographic data questionnaire was used, nutritional status was determined based on the recommendations of the World Health Organization, Family Adaptability and Cohesion Evaluation Scale and Parental Rearing Style Questionnaire were used. Descriptive and Spearman's correlation coefficient were obtained. RESULTS: Mean age of parents was 32.09 years, 60.5% of mothers were housewives and 100% of fathers had a job; mean years of schooling were 12.16, 68.9% of participants were married; 29.4% of preschoolers were overweight or obese. Regarding family functioning it was identified that 44.3% is semi-related in cohesion dimension. The parental rearing style that prevailed was the permissive with 62.3%. CONCLUSIONS: Most parents presented an imbalance in family functioning. No statistical significance was found among family functioning and parental rearing with nutritional status in preschoolers (P<.05).