Insecticidal Activity of 25 Essential Oils on the Stored Product Pest, Sitophilus granarius.

The granary weevil Sitophilus granarius is a stored product pest found worldwide. Environmental damages, human health issues and the emergence of resistance are driving scientists to seeks alternatives to synthetic insecticides for its control. With low mammal toxicity and low persistence, essential oils are more and more being considered a potential alternative. In this study, we compare the toxicity of 25 essential oils, representing a large array of chemical compositions, on adult granary weevils. Bioassays indicated that Allium sativum was the most toxic essential oil, with the lowest calculated lethal concentration 90 (LC90) both after 24 h and 7 days. Gaultheria procumbens, Mentha arvensis and Eucalyptus dives oils appeared to have a good potential in terms of toxicity/cost ratio for further development of a plant-derived biocide. Low influence of exposure time was observed for most of essential oils. The methodology developed here offers the possibility to test a large array of essential oils in the same experimental bioassay and in a standardized way. It is a first step to the development of new biocide for alternative management strategies of stored product pests.

Seasonal Effect on the Chemical Composition, Insecticidal Properties and Other Biological Activities of Zanthoxylum leprieurii Guill. & Perr. Essential oils.

This study focused, for the first time, on the evaluation of the seasonal effect on the chemical composition and biological activities of essential oils hydrodistillated from leaves, trunk bark and fruits of Zanthoxylum leprieurii (Z. leprieurii), a traditional medicinal wild plant growing in Côte d'Ivoire. The essential oils were obtained by hydrodistillation from fresh organs of Z. leprieurii growing on the same site over several months using a Clevenger-type apparatus and analyzed by gas chromatography-mass spectrometry (GC/MS). Leaf essential oils were dominated by tridecan-2-one (9.00 ± 0.02-36.80 ± 0.06%), (E)-ß-ocimene (1.30 ± 0.50-23.57 ± 0.47%), ß-caryophyllene (7.00 ± 1.02-19.85 ± 0.48%), dendrolasin (1.79 ± 0.08-16.40 ± 0.85%) and undecan-2-one (1.20 ± 0.03-8.51 ± 0.35%). Fruit essential oils were rich in ß-myrcene (16.40 ± 0.91-48.27 ± 0.26%), citronellol (1.90 ± 0.02-28.24 ± 0.10%) and geranial (5.30 ± 0.53-12.50 ± 0.47%). Tridecan-2-one (45.26 ± 0.96-78.80 ± 0.55%), ß-caryophyllene (1.80 ± 0.23-13.20 ± 0.33%), ?-humulene (4.30 ±1.09-12.73 ± 1.41%) and tridecan-2-ol (2.23 ± 0.17-10.10 ± 0.61%) were identified as major components of trunk bark oils. Statistical analyses of essential oil compositions showed that the variability mainly comes from the organs. Indeed, principal component analysis (PCA) and hierarchical cluster analysis (HCA) allowed us to cluster the samples into three groups, each one consisting of one different Z. leprieurii organ, showing that essential oils hydrodistillated from the different organs do not display the same chemical composition. However, significant differences in essential oil compositions for the same organ were highlighted during the studied period, showing the impact of the seasonal effect on essential oil compositions. Biological activities of the produced essential oils were also investigated. Essential oils exhibited high insecticidal activities against Sitophilus granarius, as well as antioxidant, anti-inflammatory and moderate anti-plasmodial properties.

Composition, Seasonal Variation, and Biological Activities of Lantana camara Essential Oils from Côte d'Ivoire.

This work aims to study the variations in the composition of Lantana camara leaf, flower, and stem essential oils over two years. L. camara organs were harvested in Bregbo (East Côte d'Ivoire) each month from June 2015 to June 2017. The essential oils were obtained by hydrodistillation and characterized by GC-MS and 13C NMR. Eighty-four compounds accounting for 84.4-99.1% of the essential oils have been identified. The essential oils hydrodistillated from L. camara are dominated by sesquiterpenes such as (E)-ß-caryophyllene and α-humulene, which were found in all samples. Some monoterpenes such as thymol, sabinene, and α-pinene were also present. Statistical analysis (principal component analysis and clustering) revealed a high variability in essential oil composition between the different organs and also within the studied periods, as the thymol proportion was higher during flowering and fruiting months. In addition, the stem, flower, and fruit essential oils were more concentrated in thymol than the leaf essential oils. The proportions of (E)-ß-caryophyllene and α-humulene were strictly inverted with the thymol proportion throughout the harvest period or vegetative cycle. The antioxidant, anti-inflammatory and insecticidal activities of leaves and flowers essential oils were also studied. Results showed that L. camara leaf and flower essential oils displayed high antioxidant, anti-inflammatory and insecticidal activities.

[Reconstruction of fractures of the anterior wall and the anterior column of the acetabulum using an ilioinguinal approach]. / Osteosynthese der Frakturen der Vorderwand und des vorderen Pfeilers des Azetabulums über einen ilioinguinalen Zugang.

OBJECTIVE: Restoration of the congruence of the hip joint. Correction of gaps or steps in the articular surface, especially in the main weight-bearing area of the acetabular dome. Correction of femoral head subluxation. Restoration of joint stability in order to enable early postoperative mobilization. INDICATIONS: Fractures of the anterior wall and/or column that are characterized by intraarticular gaps or steps of > 1 mm in the area of the main weight-bearing dome of the acetabulum. Fractures complicated by subluxation or dislocation of the femoral head. CONTRAINDICATIONS: Poor general physical condition and/or dementia. Critical soft-tissue conditions in the area near the surgical approach. Local soft-tissue infection. Preexisiting severe osteoarthritis of the hip joint. SURGICAL TECHNIQUE: Exposure of the fracture through an ilioinguinal approach. Reduction of a subluxated femoral head. Reduction of the anterior column and/or wall. Correction of articular gaps, steps and areas of joint impression. Internal fixation using small-fragment reconstruction plates, if required in combination with additional screws. POSTOPERATIVE MANAGEMENT: Postoperative radiographs for the documentation of the surgical result and implant position (exclusion of intraarticular implants). Postoperative computed tomography, if indicated. Active and passive exercises of the hip joint starting on day 1. Hip joint flexion limited to 90 degrees . Prophylaxis of thrombosis until full weight bearing, starting preoperatively. Mobilization without weight bearing or 15 kg partial weight bearing for 8-12 weeks. Progressive weight bearing over a time period of 4-6 weeks. Radiologic evaluation after 2, 6, and 12 weeks as well as after 6, 12, and 24 months. RESULTS: Excellent and good functional results are observed in 73-85% of the isolated anterior column fractures. The anterior wall fracture is a seldom injury. Functional results are worse in comparison to the other simple fracture types. Good or excellent results can only be observed in two thirds of cases. This observation is related to the fact that anterior wall fractures often occur in elderly patients with osteoporotic bone.

[Patella fracture]. / Patellafraktur.

OBJECTIVE: Reconstruction of the extensor apparatus of the knee joint. Open reduction and stable internal fixation of patella fractures. Exact reconstruction of the articular surface of the patellofemoral joint. INDICATIONS: Open and closed fractures. Fractures with an intraarticular incongruity (> 2 mm). Incapability of extension of the knee also in nondisplaced fractures of the patella. In the context of internal fixations of additional periarticular fractures of the knee joint. CONTRAINDICATIONS: Compromised general health status or associated injuries. Compromised local soft-tissue situation (contamination, poor soft-tissue cover). Relative: nondisplaced transverse fractures (no displacement in 40 degrees of flexion). Relative: nondisplaced longitudinal fractures(3). Relative: nondisplaced radiating fractures(3). Relative: nondisplaced fractures of the distal pole without involvement of the joint surface and intact extensor apparatus(3). SURGICAL TECHNIQUE: Longitudinal parapatellar incision. Tension band wiring. Lag screw osteosynthesis. Combination of tension band wiring and lag screws. Patellectomy (partial/complete). POSTOPERATIVE MANAGEMENT: Thrombosis prophylaxis. Early physiotherapy. Knee brace. RESULTS: 70% good to excellent results. 30% chronic pain and posttraumatic arthritis. Loss of motion (limited extension).

Double or triple interlocking when nailing proximal tibial fractures? A biomechanical investigation.

OBJECTIVES: To determine whether there are differences in stability between double and triple interlocked intramedullary nails used for the fixation of extraarticular proximal tibial fractures. DESIGN: Randomized in vitro biomechanical-experimental laboratory investigation. SETTING: Biomechanics laboratory of the Clinic for Trauma Surgery at the Johannes Gutenberg-University Mainz. INTERVENTION: A 10-mm defect osteotomy was performed on six paired human tibiae, and the proximal and distal ends were potted in polymethylmethacrylate cement (PMMA). Each pair of bones was randomly stabilized with an intramedullary nail (IM-nail) with two interlocking options (PTN 2s) in one tibia, and with an IM-nail with three interlocking options (PTN 3s) in the corresponding contralateral bone. A biomechanical test of the bone implant construct was then performed with an axial force of 900 N. Displacement of bone fragments was measured and depicted as a force-displacement diagram. MAIN OUTCOME MEASUREMENTS: Biomechanical construction stiffness. RESULTS: The stiffness values for PTN 3s were significantly higher than for PTN 2s. In the group of PTN 2s, two out of six implants failed biomechanically with breakage of one proximal interlocking screw. CONCLUSIONS: Given the parameters of this investigation, triple proximal interlocking provides more stability in nailed proximal tibia fractures than double proximal interlocking. Larger series with clinical follow-up after triple proximal interlocking in tibial nailing should be undertaken to further clarify these questions.

Extracorporeal shock wave-mediated changes in proliferation, differentiation, and gene expression of human osteoblasts.

BACKGROUND: The goal of this study was to determine whether cell proliferation, differentiation, and gene expression of primary human osteoblasts (hOB) are influenced by shock wave application (SWA). METHODS: Osteoblast cultures were isolated from cancellous bone fragments and treated with 500 impulses of energy flux densities of 0.06 mJ/mm, 0.18 mJ/mm, 0.36 mJ/mm, and 0.50 mJ/mm. Twenty-four hours and 96 hours after SWA cell proliferation, alkaline phosphatase activity, and mineralization were analyzed. The global gene expression profiling was determined 96 hours after SWA employing Affymetrix HG-U133A microarrays. RESULTS: After 24 hours, hOB showed a dose-dependent increase in cell proliferation from 68.7% (at 0.06 mJ/mm, p = 0.002) up to 81.6% (at 0.5 mJ/mm, p = 0.001), which also persisted after 96 hours. Numbers of alkaline phosphatase-positive hOB increased after SWA treatment with peak levels of response between 0.18 mJ/mm and 0.5 mJ/mm after 24 hours. Mineralization was significantly higher in all groups compared with controls. Microarray analyses revealed SWA-induced differential expression of 94 genes involved in physiologic processes, cell homeostasis, and bone formation. Most intriguing was the up-regulation of multiple genes involved in skeletal development and osteoblast differentiation (e.g., PTHrP, prostaglandin E2-receptor EP3, BMP-2 inducible kinase, chordin, cartilage oligomeric matrix protein, matrillin). CONCLUSION: We showed that shock waves have direct dose-dependent stimulatory effects on proliferation and differentiation of osteoblasts from normal human cancellous bone. We demonstrated that several genes critical for osteoblast differentiation and function are regulated after SWA. Overall, data presented herein will aid further understanding of the osteogenic effect of shock waves and, in addition, will enhance current knowledge of the SWA-mediated gene expression.

[Transosseous repair of patellar tendon ruptures]. / Transossäre Naht der Patellarsehnenruptur.

OBJECTIVE: Restoration of active knee extension. Restoration of active knee stabilization. Avoiding secondary patella alta. INDICATIONS: Acute rupture of the patellar tendon within 3-5 days. Chronic rupture of the patellar tendon. CONTRAINDICATIONS: Compromised general health status or associated injuries. Compromised local soft-tissue situation. SURGICAL TECHNIQUE: Exposure of the ruptured tendon. Coronal drill hole through the distal third of the patella and coronal drill hole through the tibial tuberosity. After anatomic positioning of the patella (adjusting correct height), patellotibial fixation with monofil or woven (Labitzke) cerclage wire or PDS cord. Suture repair of the patellar tendon and repair of the ruptured medial and lateral retinaculum. Drain insertion. Wound closure in layers. POSTOPERATIVE MANAGEMENT: Full load bearing in cylinder cast. Week 0-2: flexion restricted to 30 degrees , quadriceps muscle isometry. Week 2-4: flexion restricted to 60 degrees , strengthening of hip abductors and extensors. Week 4-6: flexion restricted to 90 degrees . After week 6: removal of cylinder cast. After week 12: return to sporting activities, removal of the cerclage wire. RESULTS: Good results after surgical therapy. Low rate of secondary rupture. Low rate of muscle weakness.

Intramedullary stabilization of extraarticular proximal tibial fractures: a biomechanical comparison of intramedullary and extramedullary implants including a new proximal tibia nail (PTN).

OBJECTIVES: To determine in the laboratory whether there are or are not differences between individual geometrical designs of intramedullary and extramedullary devices used for the fixation of extraarticular proximal tibial fractures. METHODS: Five devices were tested: a newly developed Proximal Tibia Nail (PTN), conventional double-plate osteosynthesis (DPO), the Less Invasive Stabilization System (LISS), an augmented Unreamed Tibial Nail with a T-stabilization-plate (UTN + TSP), and an external fixator (ExFix). A 10-mm defect osteotomy was performed on paired human tibiae, and the proximal and distal ends were potted in polymethylmethacrylate cement (PMMA). Each pair of bones was randomly stabilized with the new PTN in 1 tibia (Groups PTN1 through PTN4) and in 1 of the 4 comparative implants in the corresponding contralateral bone. A biomechanical test of the bone implant construct was then performed with a vertical axial force of 350, 600, and 900 N, a bending moment of 6 Nm and a bidirectional rotational strain of 8 Nm. Displacement of bone fragments was measured and depicted as a force-displacement diagram. RESULTS: For axial loading, significant differences were seen between the PTN 2 group compared to the LISS group (P = 0.016) and the PTN 4 group compared to the ExFix group (P = 0.016). No statistically significant differences were seen for the PTN 1 group compared to the DPO group (P = 0.125) and the PTN 3 group compared to the UTN + TSP group (P = 0.453). The bending stiffness of the PTN 1-4 groups was not significantly different from any of the 4 alternative implants. There was comparable torsional stiffness in all implant groups except for the UTN + TSP group, which was less stable and significantly different from the PTN 3 group (P = 0.016). CONCLUSIONS: Given the parameters of this investigation, the new PTN would theoretically provide the same mechanical stability as the DPO in axial loading. Higher stability in axial loading may be present when compared to the LISS or the ExFix. Further clinical investigation of this implant will determine its usefulness among proximal tibial fixation devices.

Locked plate fixation and intramedullary nailing for proximal humerus fractures: a biomechanical evaluation.

BACKGROUND: Internal fixation of proximal humeral fractures is associated with a considerable secondary malalignment rate. Fixed-angle implants have been suggested to increase the stability of fixation. METHODS: The biomechanical properties of four different implants were tested. These included an internal fixator with semi-elastic properties (reference), the Synthes T-plate, a locked plate with rigid properties and a spiral blade locked intramedullary nail (PHN). These implants were assessed in twenty-four osteotomized pairs of human cadaveric humeri. Specimens were subjected to two-hundred cycles of axial loading and torque followed by load to failure. RESULTS: The PHN had greater axial stiffness than the reference and the T-plate. During torque, all implants were stiffer than the reference. During cyclic loading, there were no differences between the T-plate and the reference. Both the rigid internal fixator and the PHN had less irreversible deformation than the reference. Both implants resisted higher loads before failure. CONCLUSION: This study showed that the proximal humeral nail and the rigid internal fixator are stronger than the semi-elastic locked plate and the Synthes T-plate for unstable subcapital proximal humeral fractures.