Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia.

Leishmaniasis is a significant public health concern, with dogs as the primary reservoir in urban scenarios and facilitating transmission. Diagnosing infected dogs is a crucial step for public health interventions, and the development of new diagnostic platforms can significantly enhance efforts in various regions worldwide. Given the limited availability of diagnostic methods in Colombia, this study evaluates the effectiveness of an Indirect Enzyme-Linked Immunosorbent Assay (ELISA) based on the recombinant protein rLicNTPDase-2 to detect Leishmania in infected dogs. Serum samples were collected from dogs in both endemic and non-endemic areas and classified as natural standards based on prior parasitological diagnoses. The results revealed 24 true positives (TP) and 9 true negatives (TN). Subsequently, the test was then validated with samples from symptomatic and asymptomatic animals, alongside the standards, yielding a specificity of 96 %, a sensitivity of 81 %, efficiency of 90.6 %, a positive predictive value of 92.8 %, and a negative predictive value of 89.6 %. The positive likelihood ratio (RV+) was 20, while the negative likelihood ratio (RV-) was 0.19, indicating high relevance and a robust clinical utility. The area under the curve (AUC) was 1.00, suggesting that the test has excellent discriminatory ability, significantly deviating from the reference diagonal. This is further supported by the significant difference(p < 0.0001) between TN and TP results determined by Fisher's exact test. Involving 163 animals showed 47 % positive and 46 % negative results with a significant difference (p < 0.05) in the mean optical density (OD) values between positive and negative samples. These findings indicate that the ELISA test effectively differentiates between positive and negative samples based on OD values. This study suggests that ELISA based on the recombinant antigen rLicNTPDase-2 could serve as a viable alternative for the serodiagnosis of leishmaniasis in canines in Colombia.

Austrian Syndrome: Community-Acquired Pneumonia in an Unusual Triad.

Austrian syndrome corresponds to the triad of meningitis, pneumonia, and endocarditis caused by Streptococcus pneumoniae, there is no global or local incidence given the infrequency of entity. Scarce cases are published in Latin America, with none of them in Colombia. A case of Austrian syndrome by penicillin-resistant S. pneumoniae in an immunocompetent patient is presented. Aortic valve is the most frequent site involved in Austrian syndrome; this patient had an unusual localization of the vegetation on the right coronary artery ostium. The prognosis is poor with a mortality rate of 30% or higher, this patient survived despite systemic complications. Vaccination status impacts in prevention and severity of cases because responsible serotypes are often included in available vaccines. The patient had a serotype covered by available vaccines; however, her vaccination status was unknown. Thus, we present the first case reported in Colombia of Austrian syndrome by a penicillin-resistant S. pneumoniae, in a patient with no identified comorbidities or toxicological history, with a successful evolution.

Why don't we share data and code? Perceived barriers and benefits to public archiving practices.

The biological sciences community is increasingly recognizing the value of open, reproducible and transparent research practices for science and society at large. Despite this recognition, many researchers fail to share their data and code publicly. This pattern may arise from knowledge barriers about how to archive data and code, concerns about its reuse, and misaligned career incentives. Here, we define, categorize and discuss barriers to data and code sharing that are relevant to many research fields. We explore how real and perceived barriers might be overcome or reframed in the light of the benefits relative to costs. By elucidating these barriers and the contexts in which they arise, we can take steps to mitigate them and align our actions with the goals of open science, both as individual scientists and as a scientific community.

Introducing the FAIR Principles for research software.

Research software is a fundamental and vital part of research, yet significant challenges to discoverability, productivity, quality, reproducibility, and sustainability exist. Improving the practice of scholarship is a common goal of the open science, open source, and FAIR (Findable, Accessible, Interoperable and Reusable) communities and research software is now being understood as a type of digital object to which FAIR should be applied. This emergence reflects a maturation of the research community to better understand the crucial role of FAIR research software in maximising research value. The FAIR for Research Software (FAIR4RS) Working Group has adapted the FAIR Guiding Principles to create the FAIR Principles for Research Software (FAIR4RS Principles). The contents and context of the FAIR4RS Principles are summarised here to provide the basis for discussion of their adoption. Examples of implementation by organisations are provided to share information on how to maximise the value of research outputs, and to encourage others to amplify the importance and impact of this work.

Post-COVID 19 Neurological Syndrome: a fresh challenge in neurological management.

Not applicable.

Práctica del psicoanalista en la institución: ¿análisis o sugestión? / Practice of the psychoanalyst at the institutional level: analysis or suggestion?

Resumen En el marco de la investigación titulada: Práctica de los psicoanalistas en las instituciones de salud mental en el contexto latinoamericano. Un estado del arte (2000-2013), se mantuvo como eje la pregunta por la presencia del psicoanalista en contextos diferentes al dispositivo analítico clásico, el cual se enmarca en el ámbito de lo privado. Una referencia obligada en esta vía es aquella frase freudiana que evoca la aleación entre el oro puro del análisis y el cobre de la sugestión directa. El presente texto es una elaboración al respecto y sus consecuencias para una praxis psicoanalítica en el ámbito institucional. Para ello se revisaron distintas fuentes en la obra de Freud y en la enseñanza de Lacan, que, junto con otros autores, intentan dar cuenta de las dificultades y posibilidades para que ello sea viable. Se evidencia que los detractores de esta opción se concentran en lo imaginario del dispositivo, mientras que otros más orientados por la estructura del mismo y la función deseo del analista señalan la pertinencia de esta praxis por fuera del dispositivo clásico.

Abstract Within the framework of the research entitled: "Practice of psychoanalysts in mental health institutions in the Latin American context. A state of art (2000-2013) ", the question of the presence of psychoanalysts in contexts different from the classic analytical device, which is framed in the private sphere, was kept as the axis. An obligatory reference in this way is the Freudian phrase that evokes the alloy between the pure gold of the analysis and the copper of the direct suggestion. The present text is an elaboration on this and its consequences for a psychoanalytic praxis at the institutional level. For this, different sources were reviewed in the work of Freud and the lessons of Lacan, which together with other authors attempt to account for the difficulties and possibilities for this to be viable. It is evident that the detractors of this option concentrate on the imaginary of the device, while others are more oriented by the structure of the device and the desired function that the analyst points out.