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Almost 400 years ago, Rubens copied Titian's The Fall of Man, albeit with important changes. Rubens altered Titian's original composition in numerous ways, including by changing the gaze directions of the depicted characters and adding a striking red parrot to the painting. Here, we quantify the impact of Rubens's choices on the viewer's gaze behavior. We displayed digital copies of Rubens's and Titian's artworks-as well as a version of Rubens's painting with the parrot digitally removed-on a computer screen while recording the eye movements produced by observers during free visual exploration of each image. To assess the effects of Rubens's changes to Titian's composition, we directly compared multiple gaze parameters across the different images. We found that participants gazed at Eve's face more frequently in Rubens's painting than in Titian's. In addition, gaze positions were more tightly focused for the former than for the latter, consistent with different allocations of viewer interest. We also investigated how gaze fixation on Eve's face affected the perceptual visibility of the parrot in Rubens's composition and how the parrot's presence versus its absence impacted gaze dynamics. Taken together, our results demonstrate that Rubens's critical deviations from Titian's painting have powerful effects on viewers' oculomotor behavior.
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Pinturas , Papagaios , Masculino , Animais , Humanos , Movimentos Oculares , Atenção , Fixação OcularRESUMO
A guideline is proposed that comprises the minimum items to be reported in research studies involving an eye tracker and human or non-human primate participant(s). This guideline was developed over a 3-year period using a consensus-based process via an open invitation to the international eye tracking community. This guideline will be reviewed at maximum intervals of 4 years.
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The goal of this protocol is to describe fiber-optic-bundle-coupled pre-clinical confocal laser-scanning endomicroscopy (pCLE) in its specific application to elucidate capillary blood flow effects during seizures, driven by mural cells. In vitro and in vivo cortical imaging have shown that capillary constrictions driven by pericytes can result from functional local neural activity, as well as from drug application, in healthy animals. Here, a protocol is presented on how to use pCLE to determine the role of microvascular dynamics in neural degeneration in epilepsy, at any tissue depth (specifically in the hippocampus). We describe a head restraint technique that has been adapted to record pCLE in awake animals, to address potential side-effects of anesthetics on neural activity. Using these methods, electrophysiological and imaging recordings can be conducted over several hours in deep neural structures of the brain.
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Capilares , Vigília , Camundongos , Animais , Microscopia Confocal/métodos , Endoscopia , LasersRESUMO
In this paper, we present a review of how the various aspects of any study using an eye tracker (such as the instrument, methodology, environment, participant, etc.) affect the quality of the recorded eye-tracking data and the obtained eye-movement and gaze measures. We take this review to represent the empirical foundation for reporting guidelines of any study involving an eye tracker. We compare this empirical foundation to five existing reporting guidelines and to a database of 207 published eye-tracking studies. We find that reporting guidelines vary substantially and do not match with actual reporting practices. We end by deriving a minimal, flexible reporting guideline based on empirical research (Section "An empirically based minimal reporting guideline").
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Movimentos Oculares , Tecnologia de Rastreamento Ocular , Humanos , Pesquisa EmpíricaRESUMO
When we believe misinformation, we have succumbed to an illusion: our perception or interpretation of the world does not match reality. We often trust misinformation for reasons that are unrelated to an objective, critical interpretation of the available data: Key facts go unnoticed or unreported. Overwhelming information prevents the formulation of alternative explanations. Statements become more believable every time they are repeated. Events are reframed or given "spin" to mislead audiences. In magic shows, illusionists apply similar techniques to convince spectators that false and even seemingly impossible events have happened. Yet, many magicians are "honest liars," asking audiences to suspend their disbelief only during the performance, for the sole purpose of entertainment. Magic misdirection has been studied in the lab for over a century. Psychological research has sought to understand magic from a scientific perspective and to apply the tools of magic to the understanding of cognitive and perceptual processes. More recently, neuroscientific investigations have also explored the relationship between magic illusions and their underlying brain mechanisms. We propose that the insights gained from such studies can be applied to understanding the prevalence and success of misinformation. Here, we review some of the common factors in how people experience magic during a performance and are subject to misinformation in their daily lives. Considering these factors will be important in reducing misinformation and encouraging critical thinking in society.
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Medical image interpretation is central to detecting, diagnosing, and staging cancer and many other disorders. At a time when medical imaging is being transformed by digital technologies and artificial intelligence, understanding the basic perceptual and cognitive processes underlying medical image interpretation is vital for increasing diagnosticians' accuracy and performance, improving patient outcomes, and reducing diagnostician burnout. Medical image perception remains substantially understudied. In September 2019, the National Cancer Institute convened a multidisciplinary panel of radiologists and pathologists together with researchers working in medical image perception and adjacent fields of cognition and perception for the "Cognition and Medical Image Perception Think Tank." The Think Tank's key objectives were to identify critical unsolved problems related to visual perception in pathology and radiology from the perspective of diagnosticians, discuss how these clinically relevant questions could be addressed through cognitive and perception research, identify barriers and solutions for transdisciplinary collaborations, define ways to elevate the profile of cognition and perception research within the medical image community, determine the greatest needs to advance medical image perception, and outline future goals and strategies to evaluate progress. The Think Tank emphasized diagnosticians' perspectives as the crucial starting point for medical image perception research, with diagnosticians describing their interpretation process and identifying perceptual and cognitive problems that arise. This article reports the deliberations of the Think Tank participants to address these objectives and highlight opportunities to expand research on medical image perception.
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Inteligência Artificial , Radiologia , Cognição , Diagnóstico por Imagem , Humanos , Radiologia/métodos , Percepção VisualRESUMO
Research has not yet quantified the effects of workload or duty hours on the accuracy of radiologists. With the exception of a brief reduction in imaging studies during the 2020 peak of the COVID-19 pandemic, the workload of radiologists in the United States has seen relentless growth in recent years. One concern is that this increased demand could lead to reduced accuracy. Behavioral studies in species ranging from insects to humans have shown that decision speed is inversely correlated to decision accuracy. A potential solution is to institute workload and duty limits to optimize radiologist performance and patient safety. The concern, however, is that any prescribed mandated limits would be arbitrary and thus no more advantageous than allowing radiologists to self-regulate. Specific studies have been proposed to determine whether limits reduce error, and if so, to provide a principled basis for such limits. This could determine the precise susceptibility of individual radiologists to medical error as a function of speed during image viewing, the maximum number of studies that could be read during a work shift, and the appropriate shift duration as a function of time of day. Before principled recommendations for restrictions are made, however, it is important to understand how radiologists function both optimally and at the margins of adequate performance. This study examines the relationship between interpretation speed and error rates in radiology, the potential influence of artificial intelligence on reading speed and error rates, and the possible outcomes of imposed limits on both caseload and duty hours. This review concludes that the scientific evidence needed to make meaningful rules is lacking and notes that regulating workloads without scientific principles can be more harmful than not regulating at all.
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COVID-19 , Radiologia , Inteligência Artificial , Humanos , Pandemias , Radiologistas , Estados Unidos , Carga de TrabalhoRESUMO
Humans perceive millions of colors along three dimensions of color space: hue, lightness, and chroma. A major gap in knowledge is where the brain represents these specific dimensions in cortex, and how they relate to each other. Previous studies have shown that brain areas V4 and the posterior inferotemporal cortex (PIT) are central to computing color dimensions. To determine the contribution of V1 to setting up these downstream processing mechanisms, we studied cortical color responses in macaques-who share color vision mechanisms with humans. We used two-photon calcium imaging at both meso- and micro-scales and found that hue and lightness are laid out in orthogonal directions on the cortical map, with chroma represented by the strength of neuronal responses, as previously shown in PIT. These findings suggest that the earliest cortical stages of vision determine the three primary dimensions of human color perception.
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Encéfalo , Macaca , Animais , Cálcio , HumanosRESUMO
For millennia, people have used "averted vision" to improve their detection of faint celestial objects, a technique first documented around 325 BCE. Yet, no studies have assessed gaze location during averted vision to determine what pattern best facilitates perception. Here, we characterized averted vision while recording eye-positions of dark-adapted human participants, for the first time. We simulated stars of apparent magnitudes 3.3 and 3.5, matching their brightness to Megrez (the dimmest star in the Big Dipper) and Tau Ceti. Participants indicated whether each star was visible from a series of fixation locations, providing a comprehensive map of detection performance in all directions. Contrary to prior predictions, maximum detection was first achieved at ~8° from the star, much closer to the fovea than expected from rod-cone distributions alone. These findings challenge the assumption of optimal detection at the rod density peak and provide the first systematic assessment of an age-old facet of human vision.
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Visão Ocular , HumanosRESUMO
Errors in radiologic interpretation are largely the result of failures of perception. This remains true despite the increasing use of computer-aided detection and diagnosis. We surveyed the literature on visual illusions during the viewing of radiologic images. Misperception of anatomical structures is a potential cause of error that can lead to patient harm if disease is seen when none is present. However, visual illusions can also help enhance the ability of radiologists to detect and characterize abnormalities. Indeed, radiologists have learned to exploit certain perceptual biases in diagnostic findings and as training tools. We propose that further detailed study of radiologic illusions would help clarify the mechanisms underlying radiologic performance and provide additional heuristics to improve radiologist training and reduce medical error.
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Troxler fading, the perceptual disappearance of stationary images upon sustained fixation, is common for objects with equivalent luminance to that of the background. Previous work showed that variations in microsaccadic rates underlie the perceptual vanishing and intensification of simple stimuli, such as Gabor patches. Here, we demonstrate that microsaccade dynamics also contribute to Troxler fading and intensification during the viewing of representational art. Participants fixated a small spot while viewing either a Gabor patch on a blank background, or Monet's painting "Impression, Sunrise." They continuously reported, via button press/release, whether the Gabor patch, or the sun in Monet's painting, was fading versus intensifying, while their eye movements were recorded with high precision. Microsaccade rates peaked before reports of increased visibility, and dropped before reports of decreased visibility or fading, both when viewing Gabor patches and Monet's sun. These results reveal that the relationship between microsaccade production and the reversal and prevention of Troxler fading applies not only to the viewing of contrived stimuli, but also to the observation of "Impression, Sunrise." Whether or not perceptual fading was consciously intended by Monet, our findings indicate that observers' oculomotor dynamics are a contributor to the cornerstone of Impressionism.
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Movimentos Oculares/fisiologia , Movimentos Sacádicos/fisiologia , Visão Ocular/fisiologia , Percepção Visual/fisiologia , Adolescente , Adulto , Feminino , Fixação Ocular/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estimulação Luminosa , Adulto JovemRESUMO
Supported by guidance from training during residency programs, radiologists learn clinically relevant visual features by viewing thousands of medical images. Yet the precise visual features that expert radiologists use in their clinical practice remain unknown. Identifying such features would allow the development of perceptual learning training methods targeted to the optimization of radiology training and the reduction of medical error. Here we review attempts to bridge current gaps in understanding with a focus on computational saliency models that characterize and predict gaze behavior in radiologists. There have been great strides toward the accurate prediction of relevant medical information within images, thereby facilitating the development of novel computer-aided detection and diagnostic tools. In some cases, computational models have achieved equivalent sensitivity to that of radiologists, suggesting that we may be close to identifying the underlying visual representations that radiologists use. However, because the relevant bottom-up features vary across task context and imaging modalities, it will also be necessary to identify relevant top-down factors before perceptual expertise in radiology can be fully understood. Progress along these dimensions will improve the tools available for educating new generations of radiologists, and aid in the detection of medically relevant information, ultimately improving patient health.
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Fixação Ocular/fisiologia , Aprendizagem/fisiologia , Radiologistas , Percepção Visual/fisiologia , Diagnóstico por Imagem , HumanosRESUMO
In this paper, we outline a method for surgical preparation that allows for the practical planning of a variety of neurosurgeries in NHPs solely using data extracted from magnetic resonance imaging (MRI). This protocol allows for the generation of 3D printed anatomically accurate physical models of the brain and skull, as well as an agarose gel model of the brain modeling some of the mechanical properties of the brain. These models can be extracted from MRI using brain extraction software for the model of the brain, and custom code for the model of the skull. The preparation protocol takes advantage of state-of-the-art 3D printing technology to make interfacing brains, skulls, and molds for gel brain models. The skull and brain models can be used to visualize brain tissue inside the skull with the addition of a craniotomy in the custom code, allowing for better preparation for surgeries directly involving the brain. The applications of these methods are designed for surgeries involved in neurological stimulation and recording as well as injection, but the versatility of the system allows for future expansion of the protocol, extraction techniques, and models to a wider scope of surgeries.
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Imageamento por Ressonância Magnética/métodos , Neurocirurgia/métodos , Animais , PrimatasRESUMO
The integration of synaptic inputs onto dendrites provides the basis for neuronal computation. Whereas recent studies have begun to outline the spatial organization of synaptic inputs on individual neurons, the underlying principles related to the specific neural functions are not well understood. Here we perform two-photon dendritic imaging with a genetically-encoded glutamate sensor in awake monkeys, and map the excitatory synaptic inputs on dendrites of individual V1 superficial layer neurons with high spatial and temporal resolution. We find a functional integration and trade-off between orientation-selective and color-selective inputs in basal dendrites of individual V1 neurons. Synaptic inputs on dendrites are spatially clustered by stimulus feature, but functionally scattered in multidimensional feature space, providing a potential substrate of local feature integration on dendritic branches. Furthermore, apical dendrite inputs have larger receptive fields and longer response latencies than basal dendrite inputs, suggesting a dominant role for apical dendrites in integrating feedback in visual information processing.
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Neurônios/fisiologia , Navegação Espacial , Animais , Dendritos/fisiologia , Potenciais Pós-Sinápticos Excitadores , Macaca , Modelos Neurológicos , Sinapses/fisiologiaRESUMO
Recent technical developments and increased affordability of high-speed eye tracking devices have brought microsaccades to the forefront of research in many areas of sensory, perceptual, and cognitive processes. The present thematic issue on "Microsaccades: Empirical Research and Methodological Advances" invited authors to submit original research and reviews encompassing measurements and data analyses in fundamental, translational, and applied studies. We present the first volume of this special issue, comprising 14 articles by research teams around the world. Contributions include the characterization of fixational eye movements and saccadic intrusions in neurological impairments and in visual disease, methodological developments in microsaccade detection, the measurement of fixational eye movements in applied and ecological scenarios, and advances in the current understanding of the relationship between microsaccades and cognition. When fundamental research on microsaccades experienced a renaissance at the turn of the millennium (c.f. 4), one could hardly have been so bold as to predict the manifold applications of research on fixational eye movements in clinic and practice. Through this great variety of areas of focus, some main topics emerge. One such theme is the applicability of microsaccade measures to neurological and visual disease. Whereas microsaccade quantifications have been largely limited to participants with intact visual and oculomotor systems, recent research has extended this interest into the realm of neural and ophthalmic impairment (see 1 for a review). In this volume, Becker et al analyze " Saccadic intrusions in amyotrophic lateral sclerosis (ALS)" and Kang et al study " Fixational eye movement waveforms in amblyopia", delving into the characteristics of fast and slow eye movements. Two other articles focus on how the degradation of visual information, which is relevant to many ophthalmic pathologies, affects microsaccadic features. Tang et al investigate the " Effects of visual blur on microsaccades on visual exploration" and conclude that the precision of an image on the fovea plays an important role in the calibration of microsaccade amplitudes during visual scanning. Otero-Millan et al use different kinds of visual stimuli and viewing tasks in the presence or absence of simulated scotomas, to determine the contributions of foveal and peripheral visual information to microsaccade production. They conclude that " Microsaccade generation requires a foveal anchor". The link between microsaccadic characteristics and cognitive processes has been a mainstay of microsaccade research for almost two decades, since studies in the early 2000s connected microsaccade directions to the spatial location of covert attentional cues (2 3). In the present volume, Dalmaso et al report that " Anticipation of cognitive conflict is reflected in microsaccades", providing new insights about the top-down modulation of microsaccade dynamics. Ryan et al further examine the relationship between " Microsaccades and covert attention" during the performance of a continuous, divided-attention task, and find preliminary evidence that microsaccades track the ongoing allocation of spatial attention. Krueger et al discover that microsaccade rates modulate with visual attention demands and report that " Microsaccades distinguish looking from seeing". Taking the ecological validity of microsaccade investigations one step further, Barnhart et al evaluate microsaccades during the observation of magic tricks and conclude that " Microsaccades reflect the dynamics of misdirected attention in magic". Two articles examine the role of individual differences and intraindividual variability over time on microsaccadic features. In " Reliability and correlates of intra-individual variability in the oculomotor system" Perquin and Bompas find evidence for intra-individual reliability over different time points, while cautioning that its use to classify self-reported individual differences remains unclear. Stafford et al provide a counterpoint in " Can microsaccade rate predict drug response?" by supporting the use of microsaccade occurrence as both a trait measure of individual differences and as a state measure of response to caffeine administration. Methodological and technical advances are the subjects of three papers in this volume. In " Motion tracking of iris features to detect small eye movements" Chaudhary and Pelz describe a new video-based eye tracking methodology that relies on higher-order iris texture features, rather than on lower-order pupil center and corneal reflection features, to detect microsaccades with high confidence. Munz et al present an open source visual analytics system called " VisME: Visual microsaccades explorer" that allows users to interactively vary microsaccade filter parameters and evaluate the resulting effects on microsaccade behavior, with the goal of promoting reproducibility in data analyses. In " What makes a microsaccade? A review of 70 years research prompts a new detection method" Hauperich et al review the microsaccade properties reported between the 1940s and today, and use the stated range of parameters to develop a novel method of microsaccade detection. Lastly, Alexander et al switch the focus from the past of microsaccade research to its future, by discussing the recent and upcoming applications of fixational eye movements to ecologically-valid and real-world scenarios. Their review " Microsaccades in applied environments: real-world applications of fixational eye movement measurements" covers the possibilities and challenges of taking microsaccade measurements out of the lab and into the field. Microsaccades have engaged the interest of scientists from different backgrounds and disciplines for many decades and will certainly continue to do so. One reason for this fascination might be microsaccades' role as a link between basic sensory processes and high-level cognitive phenomena, making them an attractive focus of interdisciplinary research and transdisciplinary applications. Thus, research on microsaccades will not only endure, but keep evolving as the present knowledge base expands. Part 2 of the special issue on microsaccades is already in progress with articles currently under review and will be published in 2021.
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Visual scene characteristics can affect various aspects of saccade and microsaccade dynamics. For example, blank visual scenes are known to elicit diminished saccade and microsaccade production, compared to natural scenes. Similarly, microsaccades are less frequent in the dark. Yet, the extent to which foveal versus peripheral visual information contribute to microsaccade production remains unclear: because microsaccade directions are biased towards covert attention locations, it follows that peripheral visual stimulation could suffice to produce regular microsaccade dynamics, even without foveal stimulation being present. Here we determined the characteristics of microsaccades as a function of foveal and/or peripheral visual stimulation, while human subjects conducted four types of oculomotor tasks (fixation, free viewing, guided viewing and passive viewing). Foveal information was either available, or made unavailable, by the presentation of simulated scotomas. We found foveal stimulation to be critical for microsaccade production, and peripheral stimulation, by itself, to be insufficient to yield normal microsaccades. In each oculomotor task, microsaccade production decreased when scotomas blocked foveal stimulation. Across comparable foveal stimulation conditions, the type of peripheral stimulation (static versus dynamic) moreover affected microsaccade production, with dynamic backgrounds resulting in lower microsaccadic rates than static backgrounds. These results indicate that a foveal visual anchor is necessary for normal microsaccade generation. Whereas peripheral visual stimulation, on its own, does not suffice for normal microsaccade production, it can nevertheless modulate microsaccadic characteristics. These findings extend our current understanding of the links between visual input and ocular motor control, and may therefore help improve the diagnosis and treatment of ophthalmic conditions that degrade central vision, such as age-related macular degeneration.
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Across a wide variety of research environments, the recording of microsaccades and other fixational eye movements has provided insight and solutions into practical problems. Here we review the literature on fixational eye movements-especially microsaccades-in applied and ecologically-valid scenarios. Recent technical advances allow noninvasive fixational eye movement recordings in real-world contexts, while observers perform a variety of tasks. Thus, fixational eye movement measures have been obtained in a host of real-world scenarios, such as in connection with driver fatigue, vestibular sensory deprivation in astronauts, and elite athletic training, among others. Here we present the state of the art in the practical applications of fixational eye movement research, examine its potential future uses, and discuss the benefits of including microsaccade measures in existing eye movement detection technologies. Current evidence supports the inclusion of fixational eye movement measures in real-world contexts, as part of the development of new or improved oculomotor assessment tools. The real-world applications of fixational eye movement measurements will only grow larger and wider as affordable high-speed and high-spatial resolution eye trackers become increasingly prevalent.