RESUMO
The Light Ion Detector for ALTEA (LIDAL) is a new instrument designed to measure flux, energy spectra and Time of Flight of ions in a space habitat. It was installed in the International Space Station (Columbus) on January 19, 2020 and it is still operating. This paper presents the results of LIDAL measurements in the first 17 months of operation (01/2020-05/2022). Particle flux, dose rate, Time of Flight and spectra are presented and studied in the three ISS orthogonal directions and in the different geomagnetic regions (high latitude, low latitude, and South Atlantic Anomaly, SAA). The results are consistent with previous measurements. Dose rates range between 1.8 nGy/s and 2.4 nGy/s, flux between 0.21 particles/(sr cm2 s) and 0.32 particles/(sr cm2 s) as measured across time and directions during the full orbit. These data offer insights concerning the radiation measurements in the ISS and demonstrate the capabilities of LIDAL as a unique tool for the measurement of space radiation in space habitats, also providing novel information relevant to assess radiation risks for astronauts.
Assuntos
Radiação Cósmica , Monitoramento de Radiação , Voo Espacial , Astronave , Atividade Solar , Monitoramento de Radiação/métodos , Doses de Radiação , ÍonsRESUMO
The data presented in this article are related to the research paper entitled "Observation of night-time emissions of the Earth in the near UV range from the International Space Station with the Mini-EUSO detector" (Remote Sensing of Environment, Volume 284, January 2023, 113336, https://doi.org/10.1016/j.rse.2022.113336). The data have been acquired with the Mini-EUSO detector, an UV telescope operating in the range 290-430 nm and located inside the International Space Station. The detector was launched in August 2019, and it has started operations from the nadir-facing UV-transparent window in the Russian Zvezda module in October 2019. The data presented here refer to 32 sessions acquired between 2019-11-19 and 2021-05-06. The instrument consists of a Fresnel-lens optical system and a focal surface composed of 36 multi-anode photomultiplier tubes, each with 64 channels, for a total of 2304 channels with single photon counting sensitivity. The telescope, with a square field-of-view of 44°, has a spatial resolution on the Earth surface of 6.3 km and saves triggered transient phenomena with a temporal resolution of 2.5 µs and 320 µs. The telescope also operates in continuous acquisition at a 40.96 ms scale. In this article, large-area night-time UV maps obtained processing the 40.96 ms data, taking averages over regions of some specific geographical areas (e.g., Europe, North America) and over the entire globe, are presented. Data are binned into 0.1° × 0.1° or 0.05° × 0.05° cells (depending on the scale of the map) over the Earth's surface. Raw data are made available in the form of tables (latitude, longitude, counts) and .kmz files (containing the .png images). These are - to the best of our knowledge - the highest sensitivity data in this wavelength range and can be of use to various disciplines.
RESUMO
Recent advances in nanotechnology applied to medicine and regenerative medicine have an enormous and unexploited potential for future space and terrestrial medical applications. The Nanoparticles and Osteoporosis (NATO) project aimed to develop innovative countermeasures for secondary osteoporosis affecting astronauts after prolonged periods in space microgravity. Calcium- and Strontium-containing hydroxyapatite nanoparticles (nCa-HAP and nSr-HAP, respectively) were previously developed and chemically characterized. This study constitutes the first investigation of the effect of the exogenous addition of nCa-HAP and nSr-HAP on bone remodeling in gravity (1 g), Random Positioning Machine (RPM) and onboard International Space Station (ISS) using human bone marrow mesenchymal stem cells (hBMMSCs). In 1 g conditions, nSr-HAP accelerated and improved the commitment of cells to differentiate towards osteoblasts, as shown by the augmented alkaline phosphatase (ALP) activity and the up-regulation of the expression of bone marker genes, supporting the increased extracellular bone matrix deposition and mineralization. The nSr-HAP treatment exerted a protective effect on the microgravity-induced reduction of ALP activity in RPM samples, and a promoting effect on the deposition of hydroxyapatite crystals in either ISS or 1 g samples. The results indicate the exogenous addition of nSr-HAP could be potentially used to deliver Sr to bone tissue and promote its regeneration, as component of bone substitute synthetic materials and additive for pharmaceutical preparation or food supplementary for systemic distribution.
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Nanopartículas/administração & dosagem , Nanopartículas/química , Osteoporose/tratamento farmacológico , Ausência de Peso/efeitos adversos , Fosfatase Alcalina/metabolismo , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Cálcio/metabolismo , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Durapatita/administração & dosagem , Durapatita/química , Humanos , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteogênese/efeitos dos fármacos , Osteoporose/metabolismo , Medicina Regenerativa/métodos , Estrôncio/metabolismo , Alicerces TeciduaisRESUMO
We studied the effects of paralytic strabismus on visual behaviour and binocularity of cortical visual mechanisms by immobilization of one eye in adult cats. Visual discrimination abilities of the immobilized eye were significantly diminished despite extensive training with one or both eyes. The deficits are not caused by the immobilization itself but appear to reflect an adaptive mechanism to deal with double vision. The deficits with the immobilized eye persisted even after section of the optic chiasm, which effectively removes the direct cortical competition of the two eyes. Single-cell electrophysiological recordings showed that cortical cellular responses are modified by the immobilization, with loss of binocularity in some cells and shifting of receptive fields of other cells that continued to respond to both eyes.
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Diplopia/fisiopatologia , Aprendizagem/fisiologia , Plasticidade Neuronal/fisiologia , Estrabismo/fisiopatologia , Visão Binocular/fisiologia , Córtex Visual/fisiologia , Potenciais de Ação/fisiologia , Adaptação Fisiológica/fisiologia , Animais , Gatos , Nervos Cranianos/fisiopatologia , Nervos Cranianos/cirurgia , Denervação , Dominância Ocular/fisiologia , Eletrofisiologia/métodos , Neurônios/fisiologia , Transtornos da Motilidade Ocular/fisiopatologia , Músculos Oculomotores/inervação , Músculos Oculomotores/fisiopatologia , Paralisia/fisiopatologia , Campos Visuais/fisiologiaRESUMO
In this study we investigated the relationship between nuclear and cell surface modifications (i.e. blebbing, phosphatidylserine [PS] and sugar residues exposure) in a monocytic cell line, U937, during apoptosis induced by oxidative stress (1 mM H2O2) or inhibition of protein synthesis (10 microg/ml puromycin). Dying cells were simultaneously observed for nuclear modifications, presence of superficial blebs and plasma membrane alterations. Morphological analysis performed by conventional fluorescence microscopy, or by transmission and scanning electron microscopy showed that the courses of nuclear and membrane alterations occured concomitantly, but the phenotype was dependent on the stage of the apoptotic process and the type of apoptogenic inducer used. The progression of apoptosis in U937 cells beyond early stages resulted in the extensive formation of blebs which concomitantly lost some typical markers of apoptosis, such as PS and sugar residues. Therefore, the modality by which the nucleus condenses, or the amount and the pattern of distribution of PS on the cell surface were, for each cell line, strictly related to the apoptogenic inducer. The morphological data reported in the present paper should lead to a more precise quantification of apoptosis by improving the detection of apoptotic cells in vivo (i.e. in tissue, organs), which is a crucial point in the evaluation of efficiency of antiproliferative drugs, such as antiblastic or immunosuppressive compounds.
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Apoptose/fisiologia , Núcleo Celular/ultraestrutura , Extensões da Superfície Celular/ultraestrutura , Apoptose/efeitos dos fármacos , Núcleo Celular/efeitos dos fármacos , Tamanho Celular/efeitos dos fármacos , Extensões da Superfície Celular/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/farmacologia , Microscopia Eletrônica de Varredura , Puromicina/farmacologia , Fatores de Tempo , Células U937RESUMO
Together with some aquatic mammals, birds exhibit a unique behavioral and electrophysiological state called "unihemispheric sleep," in which one cerebral hemisphere is awake and the other is sleeping. Slow-wave sleep in one hemisphere is associated with closure of the contralateral eye, while the eye contralateral to the awake hemisphere is open; closure of both eyes, in contrast, is associated with bihemispheric slow-wave sleep or with REM sleep. During the last few days of incubation, the chick's embryo is turned in the egg so that it occludes its left eye, whereas light entering through the shell can stimulate the right eye. Here we show that in the first two days after hatching, chicks coming from eggs incubated in the light prevalently slept with their right eye open, whereas those coming from eggs incubated in the dark prevalently slept with their left eye open. Thus, asymmetric light stimulation in the embryo can modulate the left-right direction of eye opening during post-hatching monocular sleep.
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Embrião de Galinha/fisiologia , Luz , Fenômenos Fisiológicos Oculares , Sono/fisiologia , Envelhecimento , Animais , Galinhas/fisiologiaRESUMO
We describe four protocols for evaluating the attentional skills of myopic and control subjects in target stimulus detection tasks; simple reaction times (RT) are recorded. Two protocols are designed to study both automatic and voluntary orienting of attention. Modified implicit orienting paradigms [Q. J. Exp. Psychol. 32A (1980) 2; J. Exp. Psychol. Gen. 109 (1980) 160] are used in which cues elicit a shift of attention but gaze is maintained on a central fixation point. A third paradigm is designed to study the time-course of focusing; two circles (focusing cues) of different diameters are randomly presented on a point of the visual space where attention has been previously shifted. Seven SOAs (stimulus onset asynchrony) are used. The fourth paradigm was designed to evaluate visual search; three circular arrays of four, eight and twelve stimuli are randomly presented around a fixation point and subjects have to detect the target stimulus inside one of the circles (the other circles are distracters). Since some attentional deficits are associated with myopia [Invest. Ophthalmol. Vis. Sci. 38 (1997) 1725; Cogn. Brain Res. 8 (1999) 369], these protocols could be useful both in the detection of deficits in subjects on the verge of becoming myopics and in the development of cognitive training programs to reduce attentional deficits.
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Miopia/psicologia , Percepção Espacial/fisiologia , Percepção Visual/fisiologia , Adolescente , Adulto , Humanos , Orientação/fisiologia , Estimulação LuminosaRESUMO
The control of attentional orienting was studied in children with specific reading disorder (SRD) or dyslexia, and it was compared with that of normal readers. We used the covert orienting paradigm to measure subjects' reaction times for target detection both in valid and invalid cue conditions, either in the left or in the right visual fields. In experiment 1, we investigated exogenous orienting. The cue consisted of a peripheral abrupt onset and the cue-target delay was 350 ms. As compared with normal readers, in dyslexics the cue effect was absent in the right visual field, whereas in the left visual field a greater cue effect was observed. No visual field asymmetry was found in normal readers. In experiment 2, we investigated endogenous orienting. The cue was shown centrally and the cue-target delay was 750 ms. In dyslexics and normal readers, orienting of attention was present in both visual fields. However, in the invalid condition, dyslexic children showed significantly slower reaction times in the left visual field than in the right visual field. These results were interpreted as being due to an asymmetric control of visual spatial attention, possibly related with a posterior attention mechanism deficit in the right parietal cortex and/or an interhemispheric dysfunction and/or an impairment of cerebellar functions.
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Atenção/fisiologia , Dislexia/fisiopatologia , Fixação Ocular/fisiologia , Lateralidade Funcional/fisiologia , Campos Visuais/fisiologia , Adolescente , Criança , Sinais (Psicologia) , Feminino , Humanos , Inteligência , Masculino , Orientação , Tempo de Reação/fisiologia , Leitura , Valores de Referência , Escalas de WechslerRESUMO
BACKGROUND: This study investigated the relationship between chromatin compactness, which is directly related to chromatin condensation, and DAPI uptake. Materials and Methods For the structural characterization of in situ chromatin, we used fluorescence microscopy and differential scanning calorimetry on calf thymocytes. The compactness of nuclear chromatin was altered by permeabilizing native cells with NP40 detergent. A time-dependent analysis of detergent effects was performed by acquiring nuclear images at different time intervals after permeabilization. In order to compare nuclei of different sizes, we implemented a geometrical correction in the calculation of the integrated fluorescence intensity. For a quantitative evaluation of chromatin condensation we introduced two new parameters, "average chromatin packing ratio" and "average dye spatial density." RESULTS: This approach allowed us to estimate the effects of NP40 detergent at the level of in situ chromatin. Detergent effects could be modulated by changing the ionic composition of buffer. Moreover, changes of chromatin condensation induced by detergent were inversely related to modifications of nuclear volume. CONCLUSIONS: The combination of complementary information obtained by fluorescence microscopy, supported by a proper geometrical correction, and differential calorimetry allowed us to interpret the patterns of fluorescence intensities inside the nucleus in terms of chromatin structure.
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Cromatina/fisiologia , Corantes Fluorescentes/farmacocinética , Indóis/farmacocinética , Animais , Bovinos , Coloração e Rotulagem/métodosRESUMO
Despite the recent improvement in understanding the higher-order structure of chromatin fibers, the organization of interphase chromosomes in specific nuclear domains emerged only recently and it is still controversial. This study took advantage of an integrated approach using complementary techniques in order to investigate the structure and organization of chromatin in interphase nucleus. Native CHO-K1 cells were progressively heated from 310 K to 410 K and the effects of increasing temperatures on nuclear chromatin were analyzed in situ by means of cytometric and calorimetric techniques. Distribution and organization of chromatin domains were analyzed by Fluorescence microscopy, while the mean condensation of nuclear chromatin was measured by Differential scanning calorimetry. The results show as changes of nuclear structures (envelope and matrix, namely) affect significantly organization and condensation of in situ chromatin. Moreover when volume is modified by an external force (the temperature gradient in our case) we observe significant alterations of chromatin structure. These data are in accordance with the hypothesis of an inverse relationship between nuclear volume and chromatin condensation.
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Estruturas do Núcleo Celular/química , Cromatina/ultraestrutura , Animais , Células CHO , Varredura Diferencial de Calorimetria , Estruturas do Núcleo Celular/ultraestrutura , Cromatina/química , Dicroísmo Circular , Cricetinae , Corantes Fluorescentes/química , Processamento de Imagem Assistida por Computador , Indóis/química , Microscopia de Fluorescência , TemperaturaRESUMO
We carried out three experiments with the aim of verifying a critical assumption of Kinsbourne's (Acta Psychol., 33 (1970), 193-201; Attention and Performance V, London: Academic press, (1975), pp. 81-96) 'dynamic' attentional hypothesis of hemispheric asymmetries, namely, that asymmetries arise only when subjects know in advance what type of stimulus and/or cognitive mode they are about to be engaged with. We used a paradigm modified from Posner (J. Exp. Psychol., 109 (1980), 160-174) to study the effects of non-spatial 'cognitive' cueing on hemispheric asymmetries using a lexical decision and a visuo-spatial discrimination task (acute vs. obtuse angles). While we did not find significant overall hemispheric asymmetries with the spatial material, we found a consistent advantage of the left hemisphere in the lexical decision task. In Experiment 2 where the cue was presented in central vision and only the stimuli were lateralised and in Experiment 3 where both cue and stimuli were lateralised to the same hemisphere, the left hemisphere advantage did not interact with the effect of cueing. In contrast, in Experiment 4, where only the cue was lateralised and the stimuli were centrally presented, the left hemisphere advantage in the lexical decision task emerged only following invalid cueing. While the results of Experiments 2 and 3 are not in keeping with Kinsbourne's hypothesis, the result of Experiment 4 shows that some pre-exposural mechanisms may indeed affect the emergence of hemispheric asymmetries. A differential susceptibility in 'disengaging' from the processing mode induced by an invalid cue might represent another interesting example of hemispheric difference.
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Encéfalo/fisiologia , Cognição/fisiologia , Lateralidade Funcional/fisiologia , Adulto , Sinais (Psicologia) , Tomada de Decisões/fisiologia , Humanos , Masculino , Tempo de ReaçãoRESUMO
Previous studies of hormonal and neurophysiological changes in response to psychological stress in humans have produced contrasting findings due to differing experimental procedures and consistent individual variability. Habituation effects, which influence physiological coping in response to exposure to repeated stress, need to be investigated more extensively. In the present study, twenty healthy male subjects were each exposed twice to the same psychosocial stressor (Stroop Color Word Interference task, public speaking and mental arithmetic in front of an audience) during a first session (day 1) and a second session (day 8). Plasma concentrations of norepinephrine (NE), epinephrine (EPI), adrenocorticotropic hormone (ACTH), cortisol (CORT) and prolactin (PRL) were measured immediately before the beginning of the tests and at their end, 30 min later, on both experimental days. For the total group, NE, EPI, ACTH, and CORT levels were significantly elevated, and PRL levels were significantly decreased, after stress exposure on day 1. ACTH and CORT levels showed less significant increases after stress on day 8. In contrast, NE and EPI responses to stress were not significantly blunted, and PRL response was unchanged on day 8. Cluster analysis revealed two groups of subjects who showed different habituation patterns for ACTH and CORT. The first group (n=12) of subjects showed a reduction of ACTH and CORT responses to stress on day 8. The subjects of the second group (n=8) displayed a significant increase of ACTH and cortisol in response to stress on day 8, without any habituation effect. These results increase the evidence concerning the involvement of the HPA axis and catecholamines in response to psychological stress, and suggest that possible individual differences in the neuroendocrine coping mechanisms may affect mood regulation and the state of health.
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Sistemas Neurossecretores/fisiopatologia , Estresse Psicológico/sangue , Hormônio Adrenocorticotrópico/sangue , Adulto , Epinefrina/sangue , Humanos , Hidrocortisona/sangue , Masculino , Norepinefrina/sangue , Prolactina/sangueRESUMO
In this study, we investigate whether attentional focusing, like attentional orienting, comprises two independent mechanisms. We provide direct empirical evidence in favor of the existence of two mechanisms--one exogenous, or automatic, and one endogenous, or voluntary--that play a role in adjusting the size of the focus of attention. When a new object suddenly occurs in the visual field, the focus is first automatically fitted to it, and then an endogenous effort has to be exerted to maintain attention in the focused mode. Also, we provide evidence that voluntary focusing needs a perceptual object in order to operate.
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Atenção , Automatismo/psicologia , Reconhecimento Visual de Modelos , Adulto , Feminino , Humanos , Masculino , Orientação , Psicofísica , Tempo de Reação , Percepção de TamanhoRESUMO
Orienting and focusing of visual attention are two processes strictly involved in reading. They were studied in a group of dyslexic children and normal readers. Shifting of attention by both peripheral and central visual cues was studied by means of the covert orienting paradigm. Focusing, consisting in the ability to control the size of the attentional focus, was investigated using simple reaction times in central vision. Results showed that dyslexics had a specific disability in the shifting of attention caused by a peripheral cue at short SOAs, and were also able to maintain attention focused for short periods of time only, presumably not long enough for efficient visual processing. Our results support the suggestion that visual selective attention deficits in disabled readers may be due to a specific difficulty in orienting and focusing.
Assuntos
Dislexia/fisiopatologia , Percepção Espacial/fisiologia , Percepção Visual/fisiologia , Adulto , Análise de Variância , Atenção , Criança , Sinais (Psicologia) , Feminino , Humanos , Masculino , Tempo de Reação/fisiologia , Leitura , Valores de ReferênciaRESUMO
Four experiments were conducted to ascertain whether myopia is associated with deficits of visuospatial attention. In myopic and emmetropic control subjects, we studied: (1) automatic and voluntary orienting of attention, (2) focusing of attention and (3) performance on a visual search task. The results indicated that automatic orienting was defective in myopics and their performance in visual search was less efficient than that of controls. By contrast, myopics showed no deficits in voluntary orienting and in focusing.
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Atenção/fisiologia , Miopia/psicologia , Comportamento Espacial/fisiologia , Percepção Visual/fisiologia , Análise de Variância , Humanos , Orientação/fisiologiaRESUMO
Behavioural sleep during the first 2 weeks of life was investigated in female chicks reared with an imprinting object or in social (visual) isolation. Binocular sleep tended to decrease and monocular sleep to increase with age in both rearing conditions. In chicks reared with an imprinted object. during the first week, monocular sleep with either right or left eye closure occurred with approximately the same frequency, except that on day 5 in which right eye closure dominated; during the second week, however, there was a clear bias towards more monocular sleep with left eye closure. During the second week, the pattern of monocular sleep was similar in both rearing conditions, but during the first week chicks reared with the imprinting object showed relatively more right eye closure compared to chicks reared without the imprinting object, an effect that might tentatively be associated with consolidation of imprinting memories in the left hemisphere. Binocular sleep occurred in all four body postures adopted by chicks during sleep: standing sleep, sleep with bill forward, sleep with bill on the ground, and sleep with head on the ground. Monocular sleep, in contrast, only occurred when chicks adopted the bill forward posture. When the colour of the imprinting object was suddenly changed on day 8, a striking shift towards predominant right eye closure during monocular sleep was observed. The same occurred when the imprinting object was suddenly removed from the home-cage on day 8, but not with other types of changes (i.e., when a novel different object was inserted into the home-cage or when a novel-coloured imprinting object was inserted into the home-cage together with the original one). It is argued that this phenomenon could be associated with right hemisphere involvement in response to novelty.
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Sono/fisiologia , Visão Monocular/fisiologia , Envelhecimento/fisiologia , Animais , Animais Domésticos , Galinhas , Feminino , Lateralidade Funcional , Fixação Psicológica Instintiva , Postura , Isolamento Social , Visão BinocularRESUMO
Modifications of the higher-order chromatin structure induced by polyamines have been quantitatively investigated in situ through a non-invasive biophysical approach using Differential Scanning Calorimetry and Quantitative Fluorescence Microscopy. Calorimetric and intensitometric profiles have been acquired for samples of native thymocytes, alternatively suspended in buffers, with or without natural polyamines (spermine and spermidine). The results here reported show that the structure and distribution of nuclear chromatin in situ considerably change upon the ionic composition of the environment. A quantitative analysis of this data and a comparison with previous results obtained from isolated chromatin fibers was carried out. Finally, an inverse relationship between chromatin condensation and nuclear volume was observed.
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Cromatina/química , Poliaminas/metabolismo , Dobramento de Proteína , Animais , Varredura Diferencial de Calorimetria , Bovinos , Cromatina/metabolismo , Cromatina/ultraestrutura , Microscopia de Fluorescência , Timo/citologiaRESUMO
Twelve adult split-brain cats were tested binocularly in visual tasks which had been previously learned monocularly in a two-choice paradigm. Eight experimental cats learned two opposite tasks with two eyes because contingencies of reinforcement changed with the open eye. Four control cats learned the same tasks but contingencies of reinforcement did not change with the open eye and therefore they learned the same problems with the two eyes. Thereafter, cats were submitted binocularly to the same tasks but in a free-choice paradigm. Experimental cats showed extinction of the discriminative response in 12 out of 16 binocular testings; in four the extinction criterion was not reached. In control cats no extinction behavior was observed in seven out of eight testings. It is suggested that extinction of the discriminative response in experimental cats could be caused by an inhibitory effect build-up because the two hemispheres attempted to control binocular behavior in opposite ways. Alternatively, these cats may develop a response alternative to discrimination in which one hemisphere takes the control of subcortical motor and/or attentional centers. In four testings no extinction was recorded for experimental cats and it is likely that control of those centers shifted from one hemisphere to the other every few trials.
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Corpo Caloso/cirurgia , Reconhecimento Visual de Modelos/fisiologia , Visão Binocular/fisiologia , Visão Monocular/fisiologia , Animais , Gatos , Condicionamento Psicológico/fisiologia , Extinção Psicológica/fisiologia , Lateralidade Funcional/fisiologiaRESUMO
This work investigates the structure of native calf thymus chromatin as a function of fiber length and isolation procedures by using X-ray small angle scattering technique. Two methods of chromatin isolation have been compared in order to better understand the differences reported by various authors in terms of chromatin high order structure. In addition to these experimental results the effects of shearing have also been studied. In order to explain the differences among these chromatin preparations we built several models of chromatin fibers (represented as a chain of spherical subunits) assuming increasing level of condensation at increasing salt concentrations. For all these fiber models the corresponding theoretical X-ray scattering curves have been calculated and these results have been used to explain the influence of fiber length on the scattering profiles of chromatin. The comparison between experimental and theoretical curves confirms that the high molecular weight chromatin-DNA prepared by hypotonic swelling of nuclei (without enzymatic digestion) displays a partially folded structure even at low ionic strength, whereas the low molecular weight chromatin-DNA prepared by a brief nuclease digestion appears very weakly folded at the same ionic conditions.
Assuntos
Cromatina/química , Difração de Raios X/métodos , Animais , Bovinos , Cromatina/isolamento & purificação , Simulação por Computador , Modelos Biológicos , Espalhamento de Radiação , Cloreto de Sódio , TimoRESUMO
New quantitative insights on the native high order chromatin-DNA structure existing within interphase nuclei are obtained by monitoring the effects of two common well-characterized fixatives, glutaraldehyde and ethanol/acetic acid mixture, at the level of the intranuclear DNA distribution and structures. Reproducible distinct levels of DNA fluorescence intensity and their intranuclear distribution are apparent in unfixed and fixed thymocytes by using DAPI and quantitative optical microscopy based on a charge coupled device. The fluorescent histograms correlated with the calorimetric thermograms on the very same thymocytes fixed and unfixed, establish an unequivocal baseline for the different levels of structural organization of the chromatin within the intact nucleus; namely their number, DNA packing ratio and fiber diameter. A systematic comparison among all the numerous models, being so far proposed for the quinternary and quaternary levels of DNA folding, to identifies the rope or ribbon-like and the chromonema as the ones that best fit with the in situ distribution.