Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Elife ; 4: e08149, 2015 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-26633812

RESUMO

In the inner plexiform layer (IPL) of the mouse retina, ~70 neuronal subtypes organize their neurites into an intricate laminar structure that underlies visual processing. To find recognition proteins involved in lamination, we utilized microarray data from 13 subtypes to identify differentially-expressed extracellular proteins and performed a high-throughput biochemical screen. We identified ~50 previously-unknown receptor-ligand pairs, including new interactions among members of the FLRT and Unc5 families. These proteins show laminar-restricted IPL localization and induce attraction and/or repulsion of retinal neurites in culture, placing them in an ideal position to mediate laminar targeting. Consistent with a repulsive role in arbor lamination, we observed complementary expression patterns for one interaction pair, FLRT2-Unc5C, in vivo. Starburst amacrine cells and their synaptic partners, ON-OFF direction-selective ganglion cells, express FLRT2 and are repelled by Unc5C. These data suggest a single molecular mechanism may have been co-opted by synaptic partners to ensure joint laminar restriction.


Assuntos
Comunicação Celular , Glicoproteínas de Membrana/metabolismo , Neurônios/fisiologia , Receptores de Superfície Celular/metabolismo , Retina/anatomia & histologia , Retina/fisiologia , Animais , Bioquímica/métodos , Perfilação da Expressão Gênica , Camundongos , Análise em Microsséries , Receptores de Netrina , Ligação Proteica , Mapeamento de Interação de Proteínas , Proteoma/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA