Productivity, amino acid profile, and protein bioaccessibility in heterotrophic batch cultivation of Galdieria sulphuraria.

The polyextremophilic Galdieria sulphuraria is emerging as a promising microalgal species for food applications. This work explores the potential of heterotrophically cultivated G. sulphuraria as a protein producer for human consumption. To this end, the performances of four G. sulphuraria strains grown under the same conditions were compared. Amino acid profiles varied among strains and growth phases, but all samples met FAO dietary requirements for adults. The specific growth rates were between 1.01 and 1.48 day-1. After glucose depletion, all strains showed an increase of 38-49 % in nitrogen content within 48 h, reaching 7.8-12.0 % w/w. An opposite trend was observed in protein bioaccessibility, which decreased on average from 69 % during the exponential phase to a minimum of 32 % 48 h after stationary phase, with significant differences among the strains. Therefore, selecting the appropriate strain and harvesting time is crucial for successful single-cell protein production.

Black soldier fly larvae meal and fat as a replacement for soybeans in organic broiler diets: effects on performance, body N retention, carcase and meat quality.

1. Due to the increasing global demand for more sustainably produced animal protein, there is an intensive search for feeds to replace soybeans. Black soldier fly larvae (BSFL) appear to have great potential for replacing soybeans in poultry diets. The main objective of this study was to determine if the nutritional value of BSFL is superior to soybeans when feeding organic broilers, since smaller amounts of BSFL could replace the soybean content in the feed, thus saving even more resources.2. Eighty Hubbard S757, a slow growing organic broiler type, were fattened for 63 d, spending the last 49 d on one of five diets. Two soybean cake- and soybean oil-based diets (SS, SS-) were compared with three diets based on partially defatted BSFL meal and BSFL fat from two origins (AA-, AB-, BB-). Different from diet SS, diets SS-, AA-, AB-and BB- were designed with approximately 20% less lysine and methionine. Growth (n = 16), metabolisability, body nitrogen retention, carcase and meat quality (n = 8) were evaluated.3. Broilers of the insect-based feeding groups, AA- and AB-, grew similarly well compared to those of group SS. They also retained more nitrogen in the body than those fed BB- and SS-. Breast meat yield was higher with AA- and AB- than with BB- and SS-, but still lower than with SS. Dietary variations in physicochemical meat quality were of low practical relevance. Diet BB- resulted in a more yellow skin and meat. The fatty acid profile of the breast meat lipids reflected the high lauric acid proportion of the BSFL lipids, resulting in up to 80 times higher proportions than when feeding the soybean-based diets.4. The results indicate that high-quality BSFL, depending on their origin, may indeed be superior to soybean protein, but that the meat lipids from BSFL-fed broilers can contain significant amounts of lauric acid, which, from a human nutrition perspective, could have a negative impact on meat quality.

Black soldier fly larvae meal and fat can completely replace soybean cake and oil in diets for laying hens.

Currently, there is a great interest in finding alternative protein and energy sources to replace soybean-based feeds in poultry diets. The main objective of the present study was to completely replace soybean in layer diets with defatted meal and fat from black soldier fly larvae without adverse effects. For this purpose, 5 × 10 Lohmann Brown Classic hens were fed either a soybean-based diet or diets based on defatted black soldier fly larvae meal and fat from 2 producers (1 commercial, 1 small-scale) operating with different rearing substrates, temperatures, and larvae processing methods (10 hens/diet). The data obtained included nutrient composition of larvae meals and diets, amino acid digestibility (6 hens/diet), and metabolizability, performance and egg quality (all 10 hens/diet). In addition, the acceptance of the 4 larvae-based diets was tested against the soybean-based diet in a 6-day choice feeding situation (10 hens/treatment). The nutritional value of the larvae-based diets was equivalent to the soybean-based diet in hens with a laying performance of 98%. Although average feed intake was not significantly different over the 7 experimental weeks, the diets based on larvae feeds from the small-scale production appeared to be slightly less accepted in a choice situation than the soy-based diet and those with larvae from commercial origin. This was more likely the effect of the larvae fat rather than that of the larvae protein meal. In addition, the commercial larvae material was superior to that from the small-scale production concerning supply with digestible sulfur-containing amino acids (548 vs. 511 mg/day) and lysine (792 vs. 693 mg/day), egg weight (67 vs. 63 g), daily egg mass (66 vs. 61 g/day) and, in tendency, feed efficiency. The results indicate that soybean-based feeds can be replaced completely by black soldier fly meal and fat in diets of high-performing layers. However, because of nutritional differences between the larvae materials of different origin the quality of the larvae has to be closely monitored before being used.

Rapid detection of canine parvovirus in feces using monoclonal antibodies and enzyme-linked immunosorbent assay.

Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay for the detection of canine parvovirus (CPV) antigen in fecal samples. The assay was specific for the hemagglutinating protein of CPV and detected as little as 1.5 ng of virus within a 15-minute incubation period. The use of monoclonal antibodies against 2 epitopes on the CPV antigen permitted the simultaneous addition of test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation. Clinical studies revealed greater than 95% correlation between enzyme-linked immunosorbent assay results and hemagglutination titers.

Comparison of hemagglutination and competitive enzyme-linked immunosorbent assay procedures for detecting canine parvovirus in feces.

A competitive enzyme-linked immunosorbent assay (ELISA) for detection of canine parvovirus (CPV) antigen in fecal samples was developed. Fecal samples were tested by ELISA and a direct hemagglutination assay, and the results compared. The tests gave the same results in 83% and 88% of the fecal samples, depending on whether the samples were treated with chloroform. The discrepancies were due to the fact that each test detected virus in certain fecal samples that was not detected by the other. The use of a monoclonal anti-CPV conjugate resulted in a highly specific test. The performance of the ELISA as a competitive assay also increased the reproducibility and sensitivity over that which could be obtained from a classic sandwich-type procedure.