Sublethal and Transgenerational Effects of Reduced-Risk Insecticides on Macrolophus basicornis (Hemiptera: Miridae).

Reduced-risk insecticides and mirid predators have been used to control Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in tomato crops. However, even when causing low mortality to the beneficial insects, these products might cause side effects. This study investigated the sublethal and transgenerational effects of buprofezin, cyantraniliprole, and spiromesifen on Macrolophus basicornis (Stal) (Hemiptera: Miridae). After 72 h of exposure of third-instar nymphs and adults to residues on tomato leaves, adult couples were formed and kept in cages with residue-free tomato leaves. The leaves were changed every 48 h and the offspring were assessed in 6 different periods. Body size was assessed by measuring the hind-tibia length of adults (F0) from exposed nymphs and in three different offspring groups. None of the insecticide residues caused a reduction on offspring populations or affected the body size of adults in generation F0. Regardless, buprofezin and spiromesifen reduced the tibia length of adults (F1) from exposed nymphs assayed in the third mating period. Cyantraniliprole did not affect any parameter and could be recommended for control of B. tabaci in association with M. basicornis releases. This study may contribute to future field assays of the compatibility of these insecticides with M. basicornis.

Effect of Systemic Insecticides Applied via Drench on the Mortality of Diaphorina citri on Curry Leaf.

Huanglongbing (HLB), the most serious disease in citriculture, is caused by the bacteria Candidatus Liberibacter spp., which is transmitted by the Asian citrus psyllid (ACP) Diaphorina citri. HLB is mainly controlled with insecticides, necessitating the development of alternative methods, e.g., the use of trap plants such as curry leaf Bergera koenigii, which is highly attractive to the ACP. We evaluated the effects of the main systemic insecticides used by citrus growers, applied via drench to adults of D. citri on the curry leaf tree. We tested the persistence of three pesticides: thiamethoxam, thiamethoxam + chlorantraniliprole, and imidacloprid in protected cultivation and the field condition at 7, 14, 28, 42, 56, 70, 98, and 154 days after the application. Different concentrations of insecticides containing the active ingredient thiamethoxam were tested on adults to determine the LC10 and LC50. Finally, we assessed the sublethal effects on the oviposition and development of D. citri. The insecticides controlled the adults for long periods. However, in the field experiment, from 42 days after application there was a decrease in mortality caused by pesticides applied via drench, while in the protected cultivation, mortality did not decline until the last day of evaluation. The median lethal concentration (LC50) for thiamethoxam was 0.031 g of active ingredient per plant, and for thiamethoxam in a mixture, the LC50 was 0.028 g a.i. per plant. In the experiment with sublethal doses, D. citri did not oviposit on the treated plants. Our findings suggest that the attract-and-kill system using the curry leaf tree and systemic insecticides is effective for the control of D. citri and contributes to the integrated management of HLB.

Risk Assessment of Insecticides Used in Tomato to Control Whitefly on the Predator Macrolophus basicornis (Hemiptera: Miridae).

The generalist mirid predator Macrolophus basicornis may contribute to Integrated Pest Management (IPM) of Bemisia tabaci in tomato crops. It is important to know the compatibility of the chemicals used to control this pest with this promising biological control agent. Seven insecticides were tested to investigate their toxicity to the predator. For four of the products, the LC50 for adults were determined. Buprofezin, cyantraniliprole and spiromesifen did not cause lethality and were classified as harmless. Acetamiprid, bifenthrin, etofenprox + acetamiprid and pyriproxyfen + acetamiprid caused acute toxicity and were classified as harmful. LT50 for all harmful insecticides were relatively low, ranging from 1.8 to 3.2 days. Moreover, these four insecticides have low LC50, with acetamiprid (0.26 mg a.i. L-1) as the lowest, followed by bifenthrin (0.38 mg a.i. L-1), etofenprox + acetamiprid (4.80 mg a.i. L-1) and pyriproxyfen + acetamiprid (8.71 mg a.i. L-1). However, the calculated risk quotient (RQ) values demonstrated that these insecticides were mostly ecologically safe for this predator, except for acetamiprid, classified as slightly to moderately toxic. The present study can contribute to the use of M. basicornis as a biological control agent on tomato crops and to compatible use with the insecticides tested, according to IPM strategies.