Prevalence and genetic characterization of Toxoplasma gondii strains isolated from 31 wild Passeriformes collected in North-Central Oklahoma.

Passerine birds are widely distributed and adapted to various habitats, therefore they are commonly exposed to, and infected with Toxoplasma gondii. The purpose of our project was to determine the prevalence and genotypes of T. gondii in 31 different species of passerines collected as mortalities due to window collisions in North-Central Oklahoma. DNA was extracted from breast tissue and subjected to PCR with primers that amplify a portion of the T. gondii B1 gene. Genotyping was based on a portion of the infected birds based on a multiplex PCR followed by RFLP of 12 T . gondii markers. Of 103 birds comprising 31 species, the overall prevalence (95% confidence interval) of T. gondii infection was 33.0% (24.1‒42.6%). Significant differences in the proportion of T. gondii in birds according to sex or weight were not observed. However, sample sizes of each species were small and prevented a robust analysis of T. gondii according to those biological variables. Genotyping of T. gondii in a subset of 13 infected individuals of 7 species revealed 4 genotypes, according to the Toxoplasma Data Base: #54, #139, #20, and #220. Our results, while hampered by a small sample size for each bird species, suggest that infection with T. gondii in Oklahoma, is common in both migrant and resident passerines.

Diagnostic accuracy of seven radiographic views, alone and in combination, for diagnosis of pectoral girdle fractures in wild passerines after window collisions.

OBJECTIVE: To determine the prevalence of pectoral girdle fractures in wild passerines found dead following presumed window collision and evaluate the diagnostic accuracy of various radiographic views for diagnosis of pectoral girdle fractures. SAMPLE: Cadavers of 103 wild passerines that presumptively died as a result of window collisions. PROCEDURES: Seven radiographic projections (ventrodorsal, dorsoventral, lateral, and 4 oblique views) were obtained for each cadaver. A necropsy was then performed, and each bone of the pectoral girdle (coracoid, clavicle, and scapula) was evaluated for fractures. Radiographs were evaluated in a randomized order by a blinded observer, and results were compared with results of necropsy. RESULTS: Fifty-six of the 103 (54%) cadavers had ≥ 1 pectoral girdle fracture. Overall accuracy of using individual radiographic projections to diagnose pectoral girdle fractures ranged from 63.1% to 72.8%, sensitivity ranged from 21.3% to 51.1%, and specificity ranged from 85.7% to 100.0%. The sensitivity of using various combinations of radiographic projections to diagnose pectoral girdle fractures ranged from 51.1% to 66.0%; specificity ranged from 76.8% to 96.4%. CLINICAL RELEVANCE: Radiography alone appeared to have limited accuracy for diagnosing fractures of the bones of the pectoral girdle in wild passerines after collision with a window. Both individual radiographic projections and combinations of projections resulted in numerous false negative but few false positive results.

Splenic T-cell lymphoma in a North American River Otter (Lontra canadensis).

Background: Splenic lymphoma is commonly reported in domestic ferrets (Mustela putorious furo), but very rarely reported in wild Mustelidae species, including otters. One report described B-cell splenic lymphoma in an Asian small-clawed otter (Aonyx cinerea) that metastasized and was the primary reasoning for humane euthanasia (Stedman and Mills, 2014). Case Description: The current report describes a case of splenic T-cell lymphoma in a captive North American river otter (Lontra canadensis). The otter died several weeks after splenectomy and no evidence of metastasis was found on gross necropsy or histopathological evaluation. Conclusion: The splenectomy performed on this individual was presumptively curative for its splenic lymphoma. Extensive myocardial fibrosis was found, and suspected to have caused severe cardiac arrhythmia leading to acute death.