Comparative analysis of the use of natural and synthetic antioxidants in chicken meat: an update review.

The search for healthy foods has attracted the industry's attention to developing products that use natural ingredients, including natural antioxidants. Antioxidants act as free radicals or oxygen scavengers, inhibiting lipid oxidation and adversely affecting meat products' sensory and nutritional quality. Several synthetic antioxidants have been used in the meat industry; however, studies point to health risks related to their consumption. Such fact drives research into natural antioxidants extracted from grains, oilseeds, spices, fruits, and vegetables, which may have a health-promoting effect. This manuscript evaluates the effectiveness of several natural antioxidants in improving the quality and shelf life of chicken meat products during processing, storage, and distribution. The potential effects of natural antioxidants widely used in chicken products are also discussed. It can be concluded that these natural antioxidants are possible substitutes for synthetic ones. However, their use can affect the product's characteristics.

LARVICIDE ACTIVITY OF LACTOBACILLUS SPP. AND SACCHAROMYCES BOULARDII SUPERNATANTS ON TOXOCARA CANIS.

Experimental studies have demonstrated the potential of probiotics to control visceral toxocariasis, which is a tissue parasitosis that is difficult to treat. This study evaluated the in vitro activity of probiotics and their supernatants on Toxocara canis larvae. The probiotics Lactobacillus rhamnosus (ATCC 7469), Lactobacillus paracasei (ATCC 335), Saccharomyces boulardii, Saccharomyces cerevisiae, and Bacillus cereus var. toyoi were tested in the following preparations: probiotic (P) 1 × 102 to 1 × 109 colony-forming units (CFUs), inactivated probiotic (IP) 1 × 102 to 1 × 109 CFUs, supernatant probiotic (SUpP), and inactivated probiotic supernatant (SupIP). The probiotics and their respective supernatants were separately incubated with 100 T. canis larvae per well using microculture plates with RPMI-1640 medium for 48 hr at 37 C and 5% CO2. The evaluation of the in vitro tests was based on the viability of T. canis larvae, through morphologic integrity, positive motility, and the absence of trypan blue stain. Only culture supernatants (SUpP and SUpIP) of Lactobacillus spp. resulted in 100% dead larvae, whereas S. boulardii showed larvicidal activity in T. canis >70%. The rest of the tests did not show larvicide activity. Therefore, it is important to investigate the supernatant effects of Lactobacillus spp. and S. boulardii in vivo on T. canis visceral infections, their mechanisms of action, and major metabolites involved.

Supplementation with sunflower seeds in beef cattle did not impact on oocyte and in vitro embryo production.

Supplementation with compounds rich in linoleic acid, including sunflower seed supplementation, promotes increase in conception rates in cows. We aimed to evaluate whether the sunflower seed (linoleic acid source) supplementation in beef donor females alters the plasma concentrations of cholesterol, triglycerides, HDL and LDL, increases the number and quality of oocytes, increases the cleavage rates and determines an improvement in number and quality of in vitro produced blastocysts. Thus, Nelore females were divided into two groups of 15 animals to receive supplementation with or without sunflower seed for 57 days. Females underwent follicular aspiration and the oocytes were subjected to in vitro embryo production. There was no difference (p > .1) between control group and group supplemented with sunflower seed on the number of displayed follicles; number of aspired oocytes; recovery rate; cleavage rate; number of embryos; number of blastocysts; embryos number of grades I and II; plasma concentrations of total cholesterol, triglycerides; HDL and LDL. Therefore, sunflower seed supplementation in oocyte donors did not increase the number and quality of oocytes, cleavage rates and the number and quality of blastocysts produced in vitro.

Risk and other factors associated with toxoplasmosis and toxocariasis in pregnant women from southern Brazil.

Toxoplasmosis causes complications during pregnancy that have serious effects on fetal development. Thus far, toxocariasis has been reported to spread only via vertical transmission. Nonetheless, the population of pregnant women is also exposed to this infection. Co-infection with both Toxoplasma gondii and Toxocara spp. has been reported in children, but there are no reports of co-infection in the population of pregnant women. The aim of this study was to determine the prevalence of co-infection with T. gondii and Toxocara spp. in pregnant women at a university hospital in southern Brazil, and to identify the risk factors associated with infection by both parasites. Two hundred pregnant women were tested for the presence of anti-T. gondii and anti-Toxocara spp. antibodies and were asked to complete an epidemiological questionnaire. In this study, the co-infection rate observed in the total population of pregnant women was 8%. In addition, women with a positive result for a serology test for Toxocara spp. were at increased risk of infection by T. gondii (P = 0.019). Co-infection with both parasites in pregnant women was associated with low birth weights in neonates. The similar modes of transmission of both parasites could explain the co-infection. Only a few previous studies have investigated this phenomenon. The findings of the present study emphasize the importance of serological diagnosis during prenatal care and further research in this area to identify risk factors associated with this co-infection, and the possible implications of this co-infection during pregnancy and on the health of newborns.

Sensitivity of head and cervical spine injury measures to impact factors relevant to rollover crashes.

OBJECTIVE: Serious head and cervical spine injuries have been shown to occur mostly independent of one another in pure rollover crashes. In an attempt to define a dynamic rollover crash test protocol that can replicate serious injuries to the head and cervical spine, it is important to understand the conditions that are likely to produce serious injuries to these 2 body regions. The objective of this research is to analyze the effect that impact factors relevant to a rollover crash have on the injury metrics of the head and cervical spine, with a specific interest in the differentiation between independent injuries and those that are predicted to occur concomitantly. METHODS: A series of head impacts was simulated using a detailed finite element model of the human body, the Total HUman Model for Safety (THUMS), in which the impactor velocity, displacement, and direction were varied. The performance of the model was assessed against available experimental tests performed under comparable conditions. Indirect, kinematic-based, and direct, tissue-level, injury metrics were used to assess the likelihood of serious injuries to the head and cervical spine. RESULTS: The performance of the THUMS head and spine in reconstructed experimental impacts compared well to reported values. All impact factors were significantly associated with injury measures for both the head and cervical spine. Increases in impact velocity and displacement resulted in increases in nearly all injury measures, whereas impactor orientation had opposite effects on brain and cervical spine injury metrics. The greatest cervical spine injury measures were recorded in an impact with a 15° anterior orientation. The greatest brain injury measures occurred when the impactor was at its maximum (45°) angle. CONCLUSIONS: The overall kinetic and kinematic response of the THUMS head and cervical spine in reconstructed experiment conditions compare well with reported values, although the occurrence of fractures was overpredicted. The trends in predicted head and cervical spine injury measures were analyzed for 90 simulated impact conditions. Impactor orientation was the only factor that could potentially explain the isolated nature of serious head and spine injuries under rollover crash conditions. The opposing trends of injury measures for the brain and cervical spine indicate that it is unlikely to reproduce the injuries simultaneously in a dynamic rollover test.

Comparison of novice and full-licenced driver common crash types in New South Wales, Australia, 2001-2011.

OBJECTIVE: To examine the circumstances of passenger vehicle crashes for novice licenced drivers aged 17-25 years and to compare the crash circumstances of the most common crash types for novices to a sample of full-licence drivers aged 40-49 years. METHOD: A retrospective analysis was conducted of passenger vehicle crashes involving novice and full-licenced drivers during 1 January 2001 to 31 December 2011 in New South Wales (NSW), Australia. RESULTS: There were 4113 injurious crashes of novice drivers. Almost half the novice driver crashes involved a single vehicle. Vehicle speed (33.2%), fatigue (15.6%) and alcohol (12.6%) were identified risk factors in novice driver crashes. Correspondence analysis for 4 common crash types for novice drivers revealed that the crash characteristics between novice and full-licenced drivers were similar. CONCLUSIONS: Similarities exist between novice driver and full-licenced driver crash risk for common crash types. Preventive strategies aimed at crash risk reduction for novice drivers may also benefit all drivers.

Head and spine injuries sustained by motorcyclists in head-leading collisions with fixed roadside objects.

OBJECTIVE: Motorcyclist collisions with fixed objects account for a substantial proportion of fatalities in many countries. Biomechanically valid crash test protocols are required to assess the injury potential of different fixed objects to motorcyclists and/or to develop safety devices that ameliorate this injury risk. The aim of the present article is to provide field-observed injury data pertaining to motorcyclist head-leading collisions with fixed objects to assist in the development of crash test protocols. METHOD: The Australian National Coronial Information System was used to identify fatal motorcyclist head-leading collisions with fixed objects. Head and spine injuries were identified from the autopsy reports for these individuals. The head impact locations and injuries were used to infer impact orientations and corresponding injury mechanisms. RESULTS: A sample of 44 motorcyclists estimated to have impacted fixed objects in the head-leading orientation was identified. The analysis of autopsy reports indicated a predominance of basilar skull fractures, intracranial injuries to the frontal cerebrum and inferior aspects of the brain (brainstem and cerebellum), and upper cervical spine injuries. Analysis of head impact locations identified a predominance of impacts to the frontal and/or lateral aspects and when considered in combination with the injury mechanisms, a typical impact orientation of sliding in the prone position with head extension was inferred. CONCLUSIONS: The study results were used to suggest possible crash test protocols for motorcyclists sliding into fixed objects and/or safety devices designed to reduce the injury risk of fixed objects. The predominant orientation of lying prone with head extension led the authors to suggest a crash test using the motorcycle anthropomorphic test device (MATD) sliding prone. However, the occurrence of the supine orientation, albeit less frequent, indicates the utility of a crash test with an anthropomorphic test device (ATD) sliding supine. The 2 options are discussed, particularly with regard to appropriate injury assessment reference values.

Eletroacupuntura para tratamento de hipotensão induzida por isofluorano em cavalos / Electroacupuncture treatment for isoflurane induced hypotension in horses

Objetivou-se avaliar a eficiência do tratamento da hipotensão arterial com eletroacupuntura comparativamente à dobutamina em equinos. Foram avaliados seis cavalos adultos, saudáveis, mantidos em anestesia inalatória, com isofluorano, em ventilação mecânica. Após a estabilização da anestesia, foi induzida hipotensão arterial, através do incremento da concentração do isofluorano, iniciando-se um dos tratamentos: DOB: dobutamina (1,5µg kg-1 min-1, infusão contínua intravenosa); EA: estímulo elétrico no acuponto pericárdio 6 (PC6), bilateralmente; SHAM: estímulo elétrico em ponto falso de acupuntura. Foram mensurados: frequência cardíaca (FC), pressão arterial média (PAM), temperatura retal (T), concentração final expirada de isofluorano (ETiso), variáveis hemogasométricas, concentração sérica de aspartato aminotransferase (AST) e creatina fosfoquinase (CK), tempo e qualidade da recuperação pós-anestésica. Houve incremento na PAM de 50%, 36,6% e 7,5% nos tratamentos DOB, EA e SHAM, respectivamente. Não houve diferença entre os grupos nas variáveis hemogasométricas, FC, T, ETiso, CK, AST, tempo e qualidade de recuperação pós-anestésica. Conclui-se que o tratamento com dobutamina foi mais efetivo para o tratamento da hipotensão em cavalos sob anestesia inalatória quando comparado ao estímulo elétrico do acuponto PC6 ou ponto falso de acupuntura...

This study aimed to evaluate the efficacy of electroacupuncture compared to the dobutamine treatment of hypotension in equines. Six adult horses were maintained in isoflurane anesthesia with mechanical ventilation. After anesthesia was established, the isoflurane concentration was raised until hypotension was achieved. After that the animals were treated with a constant rate of 1.5mg kg -1min-1 intravenous dobutamine (DOB), electroacupunture to pericardium 6 (PC-6) acupoint (EA) and false point treatment (SHAM). Heart rate (HH), median arterial blood pressure (MAP), rectal temperature (T), isoflurane end-tidal concentration, arterial blood gases, creatine kinase (CK), aspartate transaminase (AST), recovery time and quality of recovery were investigated. The MAP increased 50%, 36.5% and 7.5%% in DOB, EA and SHAM treatments, respectively. HH, T, arterial blood gases, CK, AST, recovery time and quality of recovery did not differ among treatments. It was concluded that the dobutamine treatment was more effective than EA and SHAM treatments for the reversion of isoflurane induced hypotension in horses...

Head injuries to restrained occupants in single-vehicle pure rollover crashes.

OBJECTIVE: Studies performed previously of seat-belted occupants in real-world passenger vehicle rollover-only crashes have identified the head as one of the body regions most often seriously injured. However, there have been few studies investigating how these head injuries occur in any detail. This study aims to investigate the characteristics and patterns of head injury to seat-belted occupants in real-world rollover-only crashes and to identify possible biomechanical mechanisms responsible for head injury to aid in the development of a dynamic rollover test protocol. METHODS: National Automotive Sampling System-Crashworthiness Data System (NASS-CDS) data were used to generate summary statistics and perform logistic regression analysis of restrained and contained occupants in U.S. pure trip-over rollover crashes. Specific information from selected CDS cases focused on identifying potential mechanisms and patterns of serious head injury and the rollover conditions under which the injury occurred are also presented. RESULTS: Twenty-one percent of seriously injured occupants in pure trip-over rollovers had a serious head injury. On average, occupants seated on the far side of the rollover sustained serious head injuries more frequently and were more likely to receive injuries to the inboard side of the head than near-side occupants. Serious head injuries appear to be decoupled from serious injuries to other body regions except for a relationship found between basal skull fractures and cervical spine fractures. Serious head injuries were sustained by some occupants who had less than 15 cm of roof crush above their seated position. CONCLUSIONS: Serious brain injuries appear to occur frequently as a result of loading to the periphery of the head from contact with the roof assembly. Two mechanisms of injury for basal skull fractures in rollover crashes were identified. The injury patterns and locations of contact to the head are sensitive to the seated position of the occupant.

Cervical and thoracic spine injury from interactions with vehicle roofs in pure rollover crashes.

Around one third of serious injuries sustained by belted, non-ejected occupants in pure rollover crashes occur to the spine. Dynamic rollover crash test methodologies have been established in Australia and the United States, with the aims of understanding injury potential in rollovers and establishing the basis of an occupant rollover protection crashworthiness test protocol that could be adopted by consumer new car assessment programmes and government regulators internationally. However, for any proposed test protocol to be effective in reducing the high trauma burden resulting from rollover crashes, appropriate anthropomorphic devices that replicate real-world injury mechanisms and biomechanical loads are required. To date, consensus regarding the combination of anthropomorphic device and neck injury criteria for rollover crash tests has not been reached. The aim of the present study is to provide new information pertaining to the nature and mechanisms of spine injury in pure rollover crashes, and to assist in the assessment of spine injury potential in rollover crash tests. Real-world spine injury cases that resulted from pure rollover crashes in the United States between 2000 and 2009 are identified, and compared with cadaver experiments under vertical load by other authors. The analysis is restricted to contained, restrained occupants that were injured from contact with the vehicle roof structure during a pure rollover, and the role of roof intrusion in creating potential for spine injury is assessed. Recommendations for assessing the potential for spine injury in rollover occupant protection crash test protocols are made.

[Partial motor status epilepticus. Primary form provoked by eye closure]. / "Partial motor status epilepticus". Forma primária desencadeada pelo fechamento dos olhos.

We report the case of a young female patient with photosensitive primary epilepsy who presented partial motor status epilepticus provoked by the act of shutting the eyes. Clinical, EEG and neuroimage data are presented and discussed.

Aspectos microbiologicos dos espacos interdigitais dos pes. II. Microbiota aerobia potencialmente patogenica nas lesoes interdigitais dos pes. / Microbiological aspects of the interdigital spaces of toe. II. Aerobic potentially pathogenic microbiota of toe web lesions

Utilizando metodos laboratoriais rotineiros para identificacao de fungos e de bacterias aerobicas, os autores procuraram determinar a incidencia desses microorganismos potencialmente patogenicos nas lesoes intertriginosas dos pes e tambem nos espacos interdigitais normais, porem adjacentes aos lesados. Chamam a atencao para a elevada frequencia com que Staphylococcus aureus e bastonetes Gram-negativos (Pseudomonas aeruginosa e enterobacterias) foram isolados quando comparados com os fungos (dermatofitos e Candida sp) e com os difteroides fluorescentes. Sugerem que cocos Gram-positivos e bastonetes Gram-negativos devam tambem estar envolvidos nos mecanismos etiopatogenicos do "pe-de-atleta"

Aspectos microbiologicos dos espacos interdigitais dos pes. I. Microbiota aerobia dos espacos interdigitais dos pes. / Microbiological aspects of toe interdigital spaces. I. Aerobial microbiota of toe interdigital spaces

Os autores ao atualizarem o tema, investigaram a microbiota dos espacos interdigitais dos pes sadios, apenas observada com as limitacoes dos metodos laboratoriais ajustados a realidade de nossa pratica hospitalar. Para tanto, os espacos interdigitais dos pes de 25 individuos foram examinados do ponto de vista bacteriologico e micologico. Em concordancia com a literatura estrangeira, puderam concluir que basicamente a flora normal desta localizacao esta constituida pelos estafilococos coagulase-negativos (principal constituinte), difteroides nao-fluorescentes (em menor percentual), estafilococos coagulase-positivos (transitorios) e Candida sp (eventualmente)

Aspectos microbiologicos dos espacos interdigitais dos pes.III. Associacao de fungos e bacterias em lesoes intertriginosas dos pes. / Microbiological aspects of toe webs.III.Fungi and bacteria association in intertrigenous lesions of toe webs

Partindo da comprovacao de que a flora microbiana potencialmente patogenica nas lesoes intertriginosas dos pes revelou-se qualitativamente expressiva e da qual fazem parte uma grande variedade de bacterias oportunistas, os autores se propuseram a verificar de que modo esses microorganismos se associam entre si naquelas lesoes, bem como a incidencia das possiveis associacoes microbianas. Pesquisaram, ainda, a associacao microbiana nos espacos interdigitais sadios, adjacentes, porem, aos espacoes lesados

Segmental transplantation of the pancreas with and without the ligation of the duct in dogs.

The autotransplantation of a pancreas segment with ligated duct to cervical vessels, autotransplantation and allotransplantation of a pancreas segment with unligated duct to iliac vessels were carried out in three groups of dogs. The occurrence was followed of the hemorrhagic necrosis and of their complications. When ligating the duct, the mortality and the rate of occurrence of hemorrhagic necrosis were of 54 and 77% respectively. In grafts with an open duct into the abdominal cavity, for the autotransplantation the mortality and occurrence of the hemorrhagic necroses were of 33%, for the allotransplantation the mortality and autodigestion were of 11 and 45%, respectively. The cause of the hemorrhagic necrosis in animals with unligated duct of the graft was most probably represented by a hematoma. The graft rejection was characterized by an asymptotic course without damaging the recipient. The transplantation method with open duct into the abdominal cavity is promising for the clinical application.

Valproato de sodio em epilepsia. / Sodium valproate in epilepsy

Estudou-se a atividade do valproato de sodio nas epilepsias, independentemente de forma, em 30 pacientes virgens de tratamento, ou, em raros casos, com medicacoes anteriores ja suspensas por espaco de tempo suficiente para sua completa eliminacao. Houve remissao completa das crises em 10 casos (33,33%) e o total de melhoras superiores a 75% foi de 18 pacientes (60%). As evolucoes eletroencefalograficas, como de habito, nao acompanharam a evolucao clinica. A dose eficaz media situou-se ao redor de 1200 mg diarias. Efeitos colaterais impedientes do tratamento ocorreram em apenas dois casos com doses inferiores a 1.200 mg diarios. Nos demais casos, surgiram com doses maiores. As conclusoes vem trazer mais uma ampliacao das possibilidades terapeuticas do valproato de sodio

Terapeutica nao-medicamentosa das cefaleias. / Non-medical therapy of cephalalgia

O autor analisa os conceitos fisiopatogenicos das cefaleias frente as mais modernas teorias neuropsiquiatricas, sugerindo a necessidade do emprego de diferentes tecnicas psicologicas, de relaxamento, de ioga e de biofeedback principalmente naque les em que esquemas medicamentosos nao mais surtem efeitos na devida eficacia, geralmente por fatores inerentes aos proprios pacientes e aos medicamentos empregados