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1.
Prev Vet Med ; 137(Pt B): 151-153, 2017 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-27993402

RESUMO

This short communication summarizes the strengths and weaknesses of current organisational structures in government veterinary services, as well as future technical, financial and societal challenges and related necessary competencies for government veterinary services of the future as discussed by an expert panel at ISVEE2015. First, participating representatives of veterinary services of diverse geographical backgrounds and statements from the audience confirmed that non-technical skills such as ability to work in teams, adaptability to new environments and situations, social and communication skills are increasingly seen as important. The second challenge faced by veterinary services is related to capacity issues, i.e. to have enough manpower to plan and deliver according to legislation in a period of shrinking budgets. New and emerging diseases can result in sudden, massive increases in the workload of veterinary services. Technical complexity has also increased for some hazards. Staff skills in veterinary services therefore need to be continuously updated, and it is essential to establish cross technical collaboration with other sectors including food safety, public health and environment. ISVEE conferences were seen as an opportunity to provide a global platform to develop skills needed by veterinary services, now and in the future.


Assuntos
Medicina Veterinária/métodos , Competência Clínica/normas , Regulamentação Governamental , Saúde Pública , Medicina Veterinária/tendências
2.
Sci Rep ; 6: 36237, 2016 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-27812044

RESUMO

In November 2014, a Eurasian strain H5N8 highly pathogenic avian influenza virus was detected in poultry in Canada. Introduced viruses were soon detected in the United States and within six months had spread to 21 states with more than 48 million poultry affected. In an effort to study potential mechanisms of spread of the Eurasian H5 virus, the United States Department of Agriculture coordinated several epidemiologic investigations at poultry farms. As part of those efforts, we sampled synanthropic birds and mammals at five infected and five uninfected poultry farms in northwest Iowa for exposure to avian influenza viruses. Across all farms, we collected 2,627 samples from 648 individual birds and mammals. House mice were the most common mammal species captured while house sparrows, European starlings, rock pigeons, swallows, and American robins were the most commonly captured birds. A single European starling was positive for Eurasian H5 viral RNA and seropositive for antibodies reactive to the Eurasian H5 virus. Two American robins were also seropositive. No mammal species showed evidence of infection. These results indicate synanthropic species merit further scrutiny to better understand potential biosecurity risks. We propose a set of management practices aimed at reducing wildlife incursions.


Assuntos
Animais Selvagens/virologia , Surtos de Doenças/veterinária , Vírus da Influenza A Subtipo H5N2/isolamento & purificação , Vírus da Influenza A Subtipo H5N2/patogenicidade , Vírus da Influenza A Subtipo H5N8/isolamento & purificação , Vírus da Influenza A Subtipo H5N8/patogenicidade , Influenza Aviária/epidemiologia , Doenças das Aves Domésticas/epidemiologia , Animais , Anticorpos Antivirais/sangue , Aves/virologia , Canadá/epidemiologia , Surtos de Doenças/prevenção & controle , Monitoramento Epidemiológico/veterinária , Feminino , Vírus da Influenza A Subtipo H5N2/genética , Vírus da Influenza A Subtipo H5N8/genética , Influenza Aviária/prevenção & controle , Influenza Aviária/virologia , Masculino , Mamíferos/virologia , Camundongos , Doenças das Aves Domésticas/virologia , Estados Unidos/epidemiologia
4.
Prev Vet Med ; 123: 185-191, 2016 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-26603048

RESUMO

Porcine deltacoronavirus (PDCoV) was first reported in the United States (US) in February 2014. This was the second novel swine enteric coronavirus detected in the US since May 2013. In this study, we conducted retrospective testing of samples submitted to three veterinary diagnostic laboratories where qualifying biological samples were derived from previously submitted diagnostic case submissions from US commercial swine farms with a clinical history of enteric disease or from cases that had been previously tested for transmissible gastroenteritis virus, PEDV, or rotavirus. Overall, 2286 banked samples were tested from 27 States. Samples were collected in 3 separate years and in 17 different months. Test results revealed 4 positive samples, 3 collected in August 2013 and 1 collected in October 2013. In addition, a case series including 42 operations in 10 States was conducted through administration of a survey. Survey data collected included information on characteristics of swine operations that had experienced PDCoV clinical signs. Special emphasis was placed on obtaining descriptive estimates of biosecurity practices and disease status over time of each operation. Clinical signs of PDCoV were reported to be similar to those of PEDV. The average number of animals on each operation exhibiting clinical signs (morbidity) and the average number of case fatalities was greatest for suckling and weaned pigs. Average operation-level weaned pig morbidity was greatest in the first week of the outbreak while average operation-level suckling pig case fatality was greatest in the second week of the outbreak. The survey included questions regarding biosecurity practices for visitors and operation employees; trucks, equipment and drivers; and feed sources. These questions attempted to identify a likely pathway of introduction of PDCoV onto the operations surveyed.


Assuntos
Infecções por Coronavirus/veterinária , Diarreia/veterinária , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Doenças dos Suínos/epidemiologia , Animais , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/mortalidade , Infecções por Coronavirus/virologia , Diarreia/epidemiologia , Diarreia/mortalidade , Diarreia/virologia , Morbidade , Prevalência , Estudos Retrospectivos , Fatores de Risco , Suínos , Doenças dos Suínos/mortalidade , Doenças dos Suínos/virologia , Estados Unidos/epidemiologia
5.
J Vet Diagn Invest ; 25(5): 608-13, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23883666

RESUMO

Vesicular stomatitis is a viral disease primarily affecting horses and cattle when it occurs in the United States. Outbreaks in the southwestern United States occur sporadically, with initial cases typically occurring in Texas, New Mexico, or Arizona and subsequent cases occurring in a northward progression. The viruses causing vesicular stomatitis can be transmitted by direct contact of lesioned animals with other susceptible animals, but transmission is primarily through arthropod vectors. In 2012, an outbreak of vesicular stomatitis in the United States occurred that was caused by Vesicular stomatitis New Jersey virus serotype. Overall, 51 horses on 36 premises in 2 states were confirmed positive. Phylogenetic analysis of the virus indicated that it was most closely related to viruses detected in the state of Veracruz, Mexico, in 2000.


Assuntos
Surtos de Doenças/veterinária , Doenças dos Cavalos/virologia , Filogenia , Estomatite Vesicular/virologia , Vírus da Estomatite Vesicular New Jersey/isolamento & purificação , Animais , Doenças dos Cavalos/epidemiologia , Doenças dos Cavalos/transmissão , Cavalos , Insetos/virologia , RNA Viral/química , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Sudoeste dos Estados Unidos/epidemiologia , Estomatite Vesicular/epidemiologia , Estomatite Vesicular/transmissão , Vírus da Estomatite Vesicular New Jersey/genética
6.
Vet J ; 188(1): 122-4, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20434377

RESUMO

The prevalence of subclinical hypocalcemia in the transition cow is unknown. Cows with subclinical hypocalcemia have no clinical signs of hypocalcemia but may be more susceptible to other diseases. The objective of this study was to determine the prevalence of subclinical hypocalcemia in the US dairy herds. As a part of the United States Department of Agriculture's National Animal Health Monitoring System 2002 Dairy study, serum samples were collected from 1462 cows within 48 h of parturition. The samples were sorted by lactation number: 1st (n=454), 2nd (n=447), 3rd (n=291), 4th (n=166), 5th (n=72), and 6th (n=32). Subclinical hypocalcemia (<2.0 mM) increased with age and was present in 25%, 41%, 49%, 51%, 54%, and 42% of 1st-6th lactation cows, respectively. Cows with serum calcium concentrations >2.0 mM had significantly lower serum non-esterified fatty acids indicating better energy balance than those with subclinical hypocalcemia. Subclinical hypocalcemia may make cows more susceptible to secondary diseases but more research will be required to determine if this is true.


Assuntos
Doenças dos Bovinos/epidemiologia , Hipocalcemia/veterinária , Lactação/metabolismo , Fatores Etários , Animais , Cálcio/sangue , Bovinos , Metabolismo Energético/fisiologia , Ácidos Graxos não Esterificados/sangue , Feminino , Hipocalcemia/epidemiologia , Gravidez , Prevalência
8.
J Vet Diagn Invest ; 18(5): 448-58, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17037612

RESUMO

Milk and serum samples from 35 dairy herds in 17 states were evaluated for cow- and herd-level Mycobacterium avium subspecies paratuberculosis (MAP) antibody test agreement. Evaluation of 6,349 samples suggested moderate agreement between milk and serum enzyme-linked immunosorbent assay (ELISA) results, with a kappa value of 0.50. Cow-level sensitivity (Se) for 18 dairy operations with 1,921 animals was evaluated relative to fecal culture results. At the cow level, the milk ELISA relative Se was not significantly different from that of the serum ELISA (21.2 and 23.5%, respectively). Logistic regression models revealed a positive association between lactation number and milk ELISA status. Non-Holstein cows were more likely to test milk ELISA positive than Holstein cows. Cows in the first 2 weeks of lactation and after week 45 of lactation were more likely to test milk ELISA positive than cows between 3 and 12 weeks of lactation. Milk production > 80% of herd average was negatively associated with testing milk ELISA positive. Animals in the West and Midwest regions were less likely than animals in the Southeast region to test ELISA positive by either test. Estimates for herd-level sensitivity for the milk and serum ELISA, relative to fecal culture results, ranged from 56 to 83%. At the cow and herd levels, milk ELISA performed equivalent to serum ELISA using fecal culture as a reference for MAP infection and has the advantage of decreased labor costs on farms that use Dairy Herd Improvement Association testing.


Assuntos
Doenças dos Bovinos/microbiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Leite/microbiologia , Mycobacterium avium subsp. paratuberculosis/isolamento & purificação , Paratuberculose/diagnóstico , Animais , Anticorpos Antibacterianos/análise , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/diagnóstico , Estudos Transversais , Fezes/microbiologia , Feminino , Modelos Logísticos , Paratuberculose/sangue , Paratuberculose/microbiologia , Sensibilidade e Especificidade , Estatísticas não Paramétricas , Estados Unidos
10.
J Vet Diagn Invest ; 18(3): 296-9, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16789722

RESUMO

The use of sentinel chickens in establishing the negative status of commercial poultry flocks depopulated due to exotic Newcastle disease (END) is considered to be an economically beneficial process. However, the costs and benefits of using sentinel chickens in noncommercial operations are in question. The objective of this study was to use sentinel chickens to evaluate whether adequate cleaning and disinfection coupled with an appropriate time period without susceptible poultry species on the premises would eliminate END virus from a noncommercial poultry operation and preclude the need for placement of sentinels in previously infected operations before declaring them free of virus. Noncommercial poultry operations were selected from the 2002 to 2003 END outbreak database. Operations included in the study had one or more isolations of END virus (ENDV) from cloacal or oropharyngeal swabs of birds on the premises. A total of 546 birds were placed on 53 premises. All sentinel birds sampled after placements were negative by virus detection methods and serologic tests. Results of this study indicate that time and the application of appropriate cleaning and disinfection procedures will adequately mitigate the risk of viable virus persisting in noncommercial poultry operations. In the future, this information may eliminate the need for sentinel bird placement to ensure virus free status of premises before repopulation, thereby decreasing the costs of END eradication.


Assuntos
Galinhas , Surtos de Doenças/veterinária , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/crescimento & desenvolvimento , Doenças das Aves Domésticas/virologia , Vigilância de Evento Sentinela/veterinária , Animais , Anticorpos Antivirais/sangue , California/epidemiologia , Embrião de Galinha , Surtos de Doenças/prevenção & controle , Desinfecção/normas , Testes de Hemaglutinação/veterinária , Doença de Newcastle/economia , Doença de Newcastle/epidemiologia , Doença de Newcastle/prevenção & controle , Vírus da Doença de Newcastle/genética , Doenças das Aves Domésticas/economia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/prevenção & controle , RNA Viral/química , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Organismos Livres de Patógenos Específicos
11.
J Am Vet Med Assoc ; 227(12): 1975-81, 2005 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-16379637

RESUMO

OBJECTIVE: To determine effects on production and risk of removal related to Mycobacterium avium subsp paratuberculosis (MAP) infection at the individual animal level in dairy cattle. DESIGN: Longitudinal study. ANIMALS: 7,879 dairy cows from 38 herds in 16 states. PROCEDURE: A subset of dairy cattle operations that participated in the National Animal Health Monitoring System Dairy 2002 study was evaluated via a serum ELISA for antibodies against MAP and categorized according to ELISA score. Dairy Herd Improvement Association records were obtained to collect current and historical lactation data and removal (ie, culling) information. Production variables were evaluated on the basis of serum ELISA category. RESULTS: Cows with strong positive results had mature equivalent (ME) 305-day milk production, ME 305-day maximum milk production, and total lifetime milk production that were significantly lower than cows in other categories. No differences were observed for ME 305-day fat and protein percentages, age, lactation, and lactation mean linear somatic cell count score between cows with strong positive results and those with negative results. After accounting for lactation number and relative herd-level milk production, cows with strong positive results were significantly more likely to have been removed by 1 year after testing. CONCLUSIONS AND CLINICAL RELEVANCE: Without management changes designed to reduce the farm-level prevalence of MAP infection, paratuberculosis will continue to reduce farm income by decreasing milk production and potentially increasing premature removal from the herd.


Assuntos
Doenças dos Bovinos/fisiopatologia , Ensaio de Imunoadsorção Enzimática/veterinária , Lactação/fisiologia , Leite/metabolismo , Paratuberculose/fisiopatologia , Animais , Anticorpos Antibacterianos/sangue , Bovinos , Contagem de Células/veterinária , Intervalos de Confiança , Feminino , Estudos Longitudinais , Leite/citologia , Mycobacterium avium subsp. paratuberculosis/imunologia , Razão de Chances
12.
J Am Vet Med Assoc ; 226(10): 1700-6, 2005 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-15906572

RESUMO

OBJECTIVE: To determine risk factors associated with hemorrhagic bowel syndrome (HBS) among dairy cattle in the United States and identify characteristics of HBS in individual cows. DESIGN: Cross-sectional, population-based survey. SAMPLE POPULATION: A stratified random sample of 1,013 dairy operations with > or = 30 cows located in 21 states. PROCEDURE: Information on management and animal health-related topics was collected with a questionnaire. RESULTS: HBS was estimated to have been observed on 9.1% of operations during the preceding 5 years and on 5.1% of operations during the preceding 12 months. Factors found in multivariable analysis to be associated with the occurrence of HBS during the preceding 12 months were large herd size, administration of bovine somatotropin, and routine use of milk urea nitrogen concentration to determine ration composition. Use of pasture as part of the lactating cow ration during the growing season was associated with decreased odds of HBS in operations with rolling herd average milk production < or = 20,000 lb, whereas in operations with higher milk production, use of pasture was not associated with occurrence of HBS. For individual cows with signs consistent with HBS, the third lactation was the median of the parity distribution and the median time between parturition and the onset of clinical signs was 104 days. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that management practices implemented to achieve high milk production may increase the risk of developing HBS in dairy cattle. Increased consumption of a high-energy diet seems to be the most plausible common pathway for all of the risk factors that have been described.


Assuntos
Ração Animal , Doenças dos Bovinos/epidemiologia , Indústria de Laticínios/métodos , Hemorragia Gastrointestinal/veterinária , Ração Animal/efeitos adversos , Criação de Animais Domésticos/métodos , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bovinos , Doenças dos Bovinos/etiologia , Estudos Transversais , Feminino , Hemorragia Gastrointestinal/epidemiologia , Hemorragia Gastrointestinal/etiologia , Lactação , Leite/metabolismo , Poaceae , Fatores de Risco , Síndrome , Estados Unidos/epidemiologia
13.
J Vet Diagn Invest ; 16(6): 509-14, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15586565

RESUMO

Five laboratories participated in a study to evaluate sources of variation in results from an enzyme-linked immunosorbent assay (ELISA) for antibodies against Mycobacterium avium subsp. paratuberculosis. Each laboratory repeatedly tested duplicates of a negative, positive (P), and high-positive (HP) serum sample, which were supplied by the United States Department of Agriculture: Animal and Plant Health Inspection Service: Veterinary Services, National Veterinary Services Laboratories, Ames, IA, on all 96-well microtiter plates when routinely testing other samples for M. avium subsp. paratuberculosis antibodies. These 3 sera were aliquoted and sent to the 5 participating laboratories. This study focused on variation in test results because of assay reagents and laboratory techniques and did not account for biologic variability associated with the time course of infection in cattle. Overall, results from 868 microtiter plates were used in the study. For each sample a sample-to-positive (S/P) ratio was calculated according to the manufacturer's directions. The S/ P ratio for the P sample ranged from 0.06 to 1.039 (mean = 0.466 and 0.484 for wells 1 and 2, respectively) and those for the HP sample ranged from 2.446 to 8.727 (mean = 4.027 and 3.980 for wells 1 and 2, respectively). The majority of the variation in S/P ratio for the P sample was attributed to kit lot (37.5%), followed by random (unexplained) error (27.0%), laboratory (18.3%), and kit lot by laboratory (11.9%). By eliminating plates in which the separation between negative and positive control ODs was less than 0.4, the proportion of variation attributed to laboratory was reduced markedly. These results confirm that there is variability in M. avium subsp. paratuberculosis ELISA results and that several sources contribute to the observed variability. The study gives a relative estimate of the contribution of various sources to the overall variability observed in the M. avium subsp. paratuberculosis ELISA results with kit lot being a primary contributor. Similar data for other ELISA tests for antibodies to M. avium subsp. paratuberculosis or other antigens also should be developed.


Assuntos
Anticorpos Antibacterianos/sangue , Doenças dos Bovinos/imunologia , Ensaio de Imunoadsorção Enzimática/veterinária , Mycobacterium avium subsp. paratuberculosis/imunologia , Paratuberculose/imunologia , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Feminino , Paratuberculose/microbiologia , Kit de Reagentes para Diagnóstico , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
14.
Prev Vet Med ; 58(3-4): 199-210, 2003 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-12706058

RESUMO

The occurrence of vesicular stomatitis (VS) was investigated in El Salvador through monthly visits to 12 sentinel cattle operations located in four different departments. Management, environmental, and spatial data were collected. Heifers were enrolled on the operations and were examined and bled monthly for 3 years. Two competitive ELISAs were used to detect antibodies on each sample for each serotype of VS virus (VSV). On 8 of the 12 operations, small terrestrial rodents were trapped, blood samples collected, and antibodies to both VS serotypes evaluated using a serum-neutralization test for each virus serotype. Similar to other studies of VS in Central America, the seroprevalence of the New Jersey serotype was higher than the seroprevalence to the Indiana serotype. An outbreak of VS appeared to occur in the Department of Sonsonate in the summer of 1999. We confirmed that VS is endemic in the four departments investigated in El Salvador.


Assuntos
Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Infecções por Rhabdoviridae/epidemiologia , Infecções por Rhabdoviridae/veterinária , Vigilância de Evento Sentinela/veterinária , Vesiculovirus , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Estudos de Coortes , Surtos de Doenças/veterinária , El Salvador/epidemiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Testes de Neutralização/veterinária , Projetos Piloto , Infecções por Rhabdoviridae/diagnóstico , Infecções por Rhabdoviridae/virologia , Roedores/classificação , Roedores/virologia , Estações do Ano , Estudos Soroepidemiológicos , Sorotipagem/veterinária , Estomatite/veterinária , Estomatite/virologia , Vírus da Estomatite Vesicular Indiana/isolamento & purificação
15.
Ann N Y Acad Sci ; 969: 205-9, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12381592

RESUMO

Approximately 20 sentinel premises in Colorado were visited quarterly during a 3-year prospective study to investigate the persistence of VS viruses in horses. A survey to assess management practices, health events, animal movements and environmental data was completed at each visit. Collection of serum samples and oral swabs along with a clinical examination of sentinel horses were performed at each visit. Serum samples were tested by 2 or more of 4 available serological tests. The data collected for two years (August 1998 to August 2000) are reported here. During this period there was seroconversion in 1 and 8 horses based on capture IgM tests for seroytpes New Jersey and Indiana, respectively. Kaplan-Meier curves were generated for those premises with horses that seroconverted and the mean survival time was 4.17 quarters (range 1.85-7.0). The occurrence of seroconversions during periods when no clinical disease was observed suggests the persistence of vesicular stomatitis viruses in the environment of the sentinel premises.


Assuntos
Doenças dos Cavalos/epidemiologia , Imunoglobulina M/sangue , Infecções por Rhabdoviridae/veterinária , Estomatite/veterinária , Vírus da Estomatite Vesicular Indiana , Vesiculovirus , Animais , Colorado/epidemiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Doenças dos Cavalos/transmissão , Doenças dos Cavalos/virologia , Cavalos , Estudos Longitudinais , Testes de Neutralização/veterinária , Infecções por Rhabdoviridae/epidemiologia , Infecções por Rhabdoviridae/transmissão , Infecções por Rhabdoviridae/virologia , Estudos Soroepidemiológicos , Estomatite/epidemiologia , Estomatite/virologia , Análise de Sobrevida , Vírus da Estomatite Vesicular Indiana/imunologia , Vírus da Estomatite Vesicular Indiana/isolamento & purificação
16.
Vet Clin North Am Food Anim Pract ; 18(1): 99-114, vi-vii, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12064172

RESUMO

Arthropod-borne diseases (ABD) of cattle include those pathogens transmitted mechanically and biologically from one bovine to another or from other species to cattle. This article provides examples of the more common ABD of North America and reviews strategies to prevent entry of ABD onto cattle operations and control transmission of ABD once established on cattle operations using an integrated approach.


Assuntos
Vetores Artrópodes/fisiologia , Doenças dos Bovinos/prevenção & controle , Doenças Transmissíveis/veterinária , Criação de Animais Domésticos/normas , Animais , Vetores Artrópodes/microbiologia , Vetores Artrópodes/parasitologia , Bovinos , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/parasitologia , Controle de Doenças Transmissíveis , Doenças Transmissíveis/transmissão , Reservatórios de Doenças , Exposição Ambiental , Medição de Risco , Fatores de Risco
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