RESUMO
The beta-adrenergic agonist isoproterenol increased the phosphorylation of elongation factor eEF2 in ventricular cardiomyocytes from adult rats (ARVC). Phosphorylation of eEF2 inhibits its activity, and protein synthesis was inhibited in ARVC concomitantly with increased eEF2 phosphorylation. eEF2 kinase activity in ARVC extracts was completely dependent upon Ca(2+)/calmodulin. In contrast to other cell types, however, treatments designed to raise intracellular cAMP failed to induce Ca(2+)/calmodulin-independent activity. Instead, they increased maximal eEF2 kinase activity. Similar data were obtained when partially purified ARVC eEF2 kinase was treated with cAMP-dependent protein kinase in vitro. These data suggest that ARVC possess a distinct isoform of eEF2 kinase.
Assuntos
Agonistas Adrenérgicos beta/farmacologia , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , AMP Cíclico/análogos & derivados , Ventrículos do Coração/efeitos dos fármacos , Fator 2 de Elongação de Peptídeos/metabolismo , Animais , Cálcio/farmacologia , Calmodulina/farmacologia , Células Cultivadas , AMP Cíclico/metabolismo , AMP Cíclico/farmacologia , Quinase do Fator 2 de Elongação , Ventrículos do Coração/citologia , Ventrículos do Coração/metabolismo , Isoproterenol/farmacologia , Fosforilação/efeitos dos fármacos , Biossíntese de Proteínas , Proteínas/efeitos dos fármacos , Ratos , Tionucleotídeos/farmacologia , Fatores de TempoRESUMO
Family functioning was investigated as a mediator between interparental conflict and adolescent depressed mood among adolescents living in two-parent and divorced families. Data were collected three times, with one year intervals. At the initial interview, adolescents were, on the average, 15.5 years old. Two types of interparental conflict were assessed: interparental conflict involving arguments about the adolescent, and arguments focused on the parents' behaviors. The results confirmed that family functioning mediated the effects of parent issue/interparental conflict, but not adolescent issue/interparental conflict. This was most evident for girls than boys. Implications of the findings for research and practice are discussed.
Assuntos
Conflito Psicológico , Depressão/psicologia , Pais/psicologia , Adolescente , Adulto , Criança , Depressão/diagnóstico , Divórcio/psicologia , Feminino , Seguimentos , Humanos , Masculino , Determinação da PersonalidadeAssuntos
Tireoidite Autoimune , Fatores Etários , Anticorpos/análise , Doenças Autoimunes , Pré-Escolar , Doença Crônica , Feminino , Humanos , Iodo/metabolismo , Radioisótopos do Iodo , Testes de Função Tireóidea , Tireoidite Autoimune/diagnóstico , Tireoidite Autoimune/imunologia , Tireoidite Autoimune/metabolismoRESUMO
Seven patients with chronic renal failure underwent intermittent hemodialysis for five to 37 months (111 patient-months on a twice-weekly basis) employing arteriovenous Teflon-Silastic cannulas and the modified two-layer Kiil hemodialyzer. A single-pass 37 degrees C. dialysate system has been used. One patient died of an indirectly related cause. All other patients have been successfully rehabilitated and now carry on normal activity of moderate sedentary type. Complications included recurring infection and clotting of arteriovenous cannulas. Hypertension and anemia were common complications requiring careful control. Peripheral neuropathy was noted in five of the seven patients but was of clinical significance in only one patient. Metastatic calcification, osteoporosis and urolithiasis also occurred in this patient. Peptic ulcers with hemorrhage developed in two patients. The degree of rehabilitation and psychological adjustment achieved by this group of patients strongly indicates the need for expansion of dialysis facilities and further research into the medical and economic aspects of dialysis.